Ajay Kumar
Email: ajay.biotech01@[Link]
Phone No: (+91) 750 075 3689
Want to work in a challenging environment and use my skills and abilities in best possible way to achieve more.
Professional Summary:
● Senior Technical Officer with more than 2 year experience in IVD grade recombinant antigen and
monoclonal antibodies (mAbs).
● Upstream and downstream processes for diagnostic grade recombinant protein (antigen) production.
● Downstream processing of antibodies production.
Skills:
● Cell disruption (using sonication & French ● IP injections in mice
press) ● Ascites collection from mice
● Protein(recombinant antigens) purification ● Antibody purification from ascites
● Affinity chromatography ● BCA Assay
● SDS PAGE ● ELISA
● Agarose Gel Electrophoresis ● Instruments calibration
● Bacterial culture growth ● Buffer Preparation
● Spectrophotometer ● Documentation work
● Mice handling
Work Experience:
Dhiti Life Sciences Pvt LTD October 2017 – Present
Senior Technical Officer
Antigen
● Works on upstream processes of bacterial culture.
● Media preparation and induction of culture with IPTG.
● Downstream processing of bacterial cells to harvest antigens.
● Protein purification using affinity chromatography.
● Several Analysis of protein done using SDS PAGE, BCA, western blotting and dot blot.
● Refolding of protein using sequential dialysis method.
Antibody
● Ascites collection from mice and plasma separation.
● Antibody purification from mice ascites using ammonium sulphate precipitation method.
● BCA estimation of protein (antigen and antibody).
● SDS PAGE analysis of proteins (antigen and antibody).
● Run Agarose Gel electrophoresis for cloning related work.
Other activity
● Assist in Western blotting assay.
● Assist in Dot blot assay.
● All the buffer preparation to be used for any protocol.
● Proper documentation of experiments performed.
● Inventory maintenance of lab reagents and consumables.
● SOP preparation and their implement.
Project
Topic: “Optimization”of Bioelectricity Generation with reference to dissolved Oxygen Concentration in
domestic Waste Water using Microbial Fuel Cell.
Guide:
Location: IFTM University, Moradabad, Uttar Pradesh
Academics
● B. Tech – Biotechnology (2013-17)
IFTM University, Moradabad, Uttar Pradesh
● Intermediate (2012)
State board, Uttar Pradesh
● High School (2010)
State board, Uttar Pradesh
Personal Details
● Languages: English & Hindi
● DOB: 04-Apr-1995
● Marital status: Single
● Address: Sarita Vihar, New Delhi.
I hereby declare the above mention information is true, if anything found incorrect, I’ll be sole responsible for their
consequences.
Ajay Kumar
Dhiti Life Sciences (p) ltd.