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Starch and Sugar Testing Methods

The document outlines various food tests including Benedict's reagent for reducing sugars, iodine for starch, biuret for proteins, DCPIP for vitamin C, and ethanol emulsion for fats. Each test includes a procedure and the expected positive result, such as color changes indicating the presence of the respective substances. Safety precautions are also emphasized, including wearing safety goggles and lab coats.

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13 views1 page

Starch and Sugar Testing Methods

The document outlines various food tests including Benedict's reagent for reducing sugars, iodine for starch, biuret for proteins, DCPIP for vitamin C, and ethanol emulsion for fats. Each test includes a procedure and the expected positive result, such as color changes indicating the presence of the respective substances. Safety precautions are also emphasized, including wearing safety goggles and lab coats.

Uploaded by

jsieilu
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Benedict’s Reagent

Revision Notes A test for


reducing sugars
FOOD TESTS
Iodine
A test for
starch Procedure
• Add 2cm3 of benedict’s reagent to the liquid
sample.
• Heat the sample in a hot water bath.
Positive Result
Benedict’s reagent goes through a series of colour
Procedure changes depending on the amount of reducing
sugar present. It turns from blue to green, yellow,
• Add iodine drop-wise to the sample. orange, and a brick red precipitate may form.
Positive Result
Iodine turns from orange to blue/black in the
presence of starch.
DCPIP
A test for
Biuret
vitamin C
A test for
proteins

Procedure
Procedure
• Add the sample drop-wise to DCPIP.
• Add biuret drop-wise to the liquid the sample. • Continue adding drops while shaking the
Positive Result solution.
Biuret turns from blue to violet in the presence of Positive Result
starch. DCPIP changes of from purple/pink to colourless if
vitamin C is present.
Ethanol Emulsion
A test for
fats Safety

!
Procedure Always follow basic safety
• Add 2cm3 of ethanol to the liquid sample and
shake vigorously.
procedures:
• Add 2cm3 of water to the sample. • Wear safety goggles.
Positive Result • Wear a lab coat.
A white emulsion forms if fats are present. • Tie back long hair.
For free flipped learning resources go to [Link]
Copyright A. Nixon. All rights reserved.

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