Scribd
Upload
61 views2 pages

DMLT Lab Instruments and Quality Control

The document outlines basic and advanced laboratory instruments used in DMLT, including centrifuges, colorimeters, and ELISA readers. It also discusses quality control systems in laboratories, emphasizing internal and external quality control, the use of control materials, and the importance of standard operating procedures and calibration. Additionally, it identifies common errors in laboratory testing, categorizing them as systematic or random.

Uploaded by

ld569553
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
61 views2 pages

DMLT Lab Instruments and Quality Control

The document outlines basic and advanced laboratory instruments used in DMLT, including centrifuges, colorimeters, and ELISA readers. It also discusses quality control systems in laboratories, emphasizing internal and external quality control, the use of control materials, and the importance of standard operating procedures and calibration. Additionally, it identifies common errors in laboratory testing, categorizing them as systematic or random.

Uploaded by

ld569553
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Instrumentation and Quality Control - DMLT 2nd Year

INSTRUMENTATION IN LABORATORY

Basic Lab Instruments:

- Centrifuge: Separates components based on density using centrifugal force.

- Colorimeter: Measures absorbance of specific wavelengths by a solution.

- Spectrophotometer: Advanced version of colorimeter; used for precise absorbance measurement.

- pH Meter: Measures acidity or alkalinity of a solution.

- Incubator: Maintains optimal temperature for microbial growth (usually 37°C).

- Hot Air Oven: Sterilizes glassware by dry heat (160-180°C).

- Autoclave: Sterilizes media, instruments using steam under pressure (121°C at 15 psi).

- Water Bath: Maintains constant temperature for reactions.

Advanced Instruments:

- ELISA Reader: Detects antibodies/antigens using enzyme-linked reactions.

- Electrophoresis Unit: Separates proteins/DNA based on size & charge.

- Blood Cell Counter: Automated device for counting RBC, WBC, platelets.

- Microscope: Used for viewing microscopic organisms/cells (Compound, Phase contrast, Fluorescent types).

QUALITY CONTROL IN LABORATORY

Definition:

A system to ensure accuracy, reliability, and consistency of lab results.

Types of Quality Control:

- Internal QC (IQC): Done daily within the lab to monitor performance.

- External QC (EQC): Done by external agencies to compare lab standards.

Control Materials:

- Known-value samples used to check machine accuracy (High, Normal, Low controls).

Levey-Jennings Chart:
Instrumentation and Quality Control - DMLT 2nd Year

- Graph used to monitor QC results over time.

- Helps detect trends or random errors.

Standard Operating Procedures (SOPs):

- Step-by-step instructions to ensure consistent operation of tests and instruments.

Calibration:

- Adjusting instruments to known standards to maintain accuracy.

Common Errors:

- Systematic Errors: Constant and predictable (e.g. faulty machine).

- Random Errors: Unpredictable (e.g. air bubbles, pipetting errors).

You might also like