MICROBIOLOGY 4 ANSWER
SECTION A MULTIPLE CHOICE QUESTIONS
1. Regarding urine recommended for culture
A. Collected in a clean bottle
B. Mid stream urine is preferably used
[Link] be refrigerated for days
[Link] azide is the best preservative
E. Formalin is added to activate bacteria
2. Which disinfectant is effective against viruses and
bacteria
A. Hypochlorites
B. Phenolics
[Link]
[Link]
E. Glutaraldehyde
3. Which equipment is used for sterilization by steam
under pressure
A. Incubator
B. Oven
[Link]
[Link] bath
E. Hot air oven
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4. The selective medium for isolation of Neisseria
gonorrhoea is
A. Neomycin blood agar medium
B. VCNT medium
[Link] agar medium
[Link] Thayer Martin Medium
E. Carry blair medium
5. Direct sensitivity testing can be performed on
which specimens
A. Pus, urine
B. Stool, pus, urine
[Link], positive blood culture, pus
[Link], urine, stool
E. Rectal swab, pus, urine
6. Which among of the following is not Gram positive
coccus?
A. Candida albicans
B. Streptococcus agalactiae
[Link] aureus
[Link]
E. Streptococci pyogenes
7. Staphylococci species and streptococci species
can be differentiated by
A. Culture media used, biochemical test,
susceptibility testing
B. Catalase test, growth condition
[Link] of infections, catalase test
[Link] test, sites of infection
E. Catalase test, susceptibility testing
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8. Which among of the following are the general
characteristics of Enterobacteriaceae
A. Catalase positive, oxidase positive, motile
B. Oxidase negative, motile, catalase negative
[Link] glucose and lactose, oxidase positive,
catalase negative
[Link] nitrate, catalase positive, oxidase
negative, ferment glucose
E. Motile, catalase positive, oxidase negative,
ferment lactose
9. Transport media to transport vibrio species to the
laboratory is
A. Ames transport media
B. Alkaline peptone media
[Link] F
[Link] be empty sterile bottle
E. Stuart transport media
10. The following are notifiable infection except
A. Viral haemorrhagic fever
B. Vibrio cholera
[Link] flexineri
[Link]-19
E. Bacillus anthrax
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11. Which are serological tests used for screening
of syphilis
A. TPHA
B. VDRL and RPR
[Link] test
[Link] slide agglutination
E. Agglutination
12. The following can be variation in gram stain
reactions EXCEPT
A. Cell wall damage due to antibiotic therapy
B. Excessive heat fixation of the smear
[Link] decolourization of the smear
[Link] of smear from young culture
E. Use of expired reagents especially lugol’s iodine
13. Positive result on indole test (tube method)
A. Red colour
B. Blue colour
[Link] colour
[Link] colour
E. Yellow colour
14. Which is the right temperature of the media to
add blood during blood agar preparation
A. 55-600C
B. 45-500C
C.30-450C
D.20-400C
E. 55-800C
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15. Yeast do appear under the microscope as
A. Multicellular mass
B. Microsporidium
[Link] single cells
[Link] letter arrangement
E. Segmented chains
16. The following statement is NOT TRUE
regarding klebsiella
A. Has large capsular cell wall
B. Mucoid growth
[Link] lactose fermenters
[Link] motile
E. Colonies are usually large
17. The test used to identify vibrio species is
A. Catalase
B. Ogawa
[Link]
[Link] test
E. Coagulase
18. A counter stain used in gram stain is
A. Crystal violet
B. Methylene blue
[Link] red
[Link]
E. Giemsa
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19. Which of the following is a common gram-
positive bacterium?
A. Escherichia coli
B. Klebsiella pneumoniae
[Link] marcesa
[Link] vulgaris
E. Staphylococcus aureus
20. The suitable pressure for sterilization during
autoclaving the culture media,
A. 20 PSI
B. 15 PSI
C.30 PSI
D.12 PSI
E. 121 PSI
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SECTION B MULTIPLE TRUE/FALSE QUESTIONS
1. The following are the morphological features of
fungi
a) FALSE They can be grown in cell culture
b) TRUE Most fungi are obligate or facultative
aerobes
c) FALSE They contain DNA and RNA
d) TRUE They are chemotrophic
e) TRUE Some of them are act as food
f) FALSE Proteus, candida and dermatophytic are
example of fungi
2. Kligler Iron Agar
a) FALSE The reaction are based on fermentations
of sucrose and glucose
b) TRUE Is a differential medium
c) TRUE Cracks and bubbles in the medium
indicates gas production
d) TRUE Yellow slope and yellow butt indicate
fermentation of lactose and glucose
e) FALSE A red pink slope and butt indicate
fermentation of lactose
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3. During identification of staphylococci bacteria
a) FALSE All staphylococci are coagulase positive
b) FALSE All staphylococci are coagulase negative
c) TRUE All staphylococci are catalase positive
d) FALSE Only staphylococcus aureus is coagulase
negative
e) TRUE Staphylococci saprophyticus is coagulase
negative
4. Regarding interpretation of susceptibility testing
a) TRUE Resistant: No zone of inhibition or zone
radius measures 2mm or less
b) FALSE Sensitive: zone radius wider than, equal
to or more than 3mm smaller than control
c) FALSE Intermediate: zone radius is more than
3mm smaller than the control and less than
3mm
d) TRUE Sensitive: zone radius wider than, equal to
or not more than 3mm smaller than control
e) TRUE Intermediate: zone radius is more than
3mm smaller than the control but not less than
3mm
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5. Regarding chocolate Agar
a) TRUE It contains sheep blood
b) TRUE It is used for growth of bacteria like
haemophilus
c) FALSE It shows haemolytic properties of
different bacteria
d) FALSE It is a differential media
e) TRUE It is an enriched media
SECTION C CROSS MATCHING QUESTIONS
S/N ANSWER COLUMN A COLUMN B
1 B Gram negative A. Vibrio cholerae
diplococci
2 E Gram positive B. Neisseria
cocci in chain meningitidis
3 D Gram positive [Link] subtilis
cocci in
clusters
4 H Gram negative [Link]
coccobacilli aureus
5 G Gram negative E. Streptococcus
rods pneumoniae
F. Streptococcus
pyogens
[Link] coli
[Link]
influenza
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SECTION D SHORT ANSWER QUESTIONS
1. List down methods for culture media sterilization
i. Autoclaving
ii. Inspissation
iii. Steaming at 1000C
iv. Filtration
2. Mention five causes of false positive when
performing sputum smear
i. Red staining of scratches on the glass slide
ii. Contamination of the slide or smear by
environmental mycobacterium
iii. Contamination of the slide with food particles
that are acid fast and stain red
iv. Mislabelling of slides and recording errors
v. Mix up of specimens at the laboratory
3. Highlight steps for culture and sensitivity
i. Inoculation
ii. Incubation
iii. Isolation
iv. Identification
v. Susceptibility test
4. List five colony characteristics to observe when
studying the culture growth
i. Size
ii. Shape
iii. Colour
iv. Texture
v. Growth pattern
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5. Mention five samples in microbiology section
which has to be sterile on collection
i. CSF
ii. Pus swab
iii. Stool
iv. Blood
v. Urine
IMEANDALIWA NA LESENI MBEYA Hub GROUP 31/08/2024
EDITED BY GROUP ADMINS
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