SYNTHESIS AND ANTIBACTERIAL ACTIVITY OF SOME

NOVEL SUBSTITUTED 1,3-THIAZINE DERIVATIVES

OSMANIA UNIVERSITY
HYDERABAD
The project report submitted to the college as a part of course work requirements in partial

fulfilment for the award of the degree of

BACHELOR OF PHARMACY
BY
Mr. A. Abhinav Reddy [636221881001]

Ms. T. Akhila [636221881009]

Mr. J. Madhu [636221881042]

Mr. Soumaybrata Saha [636221881073]

Ms. B. Sneha [636221881085]

Mr. B. Srinivas [636221881090]

Under the guidance of

Dr. B. RAVINDAR M. Pharm, Ph. D

Associate Professor
Department of Pharmaceutical Chemistry

SRIKRUPA INSTITUTE OF PHARMACEUTICAL SCIENCES

(Affiliated to Osmania University & Approved by AICTE/PCI, New Delhi)
Vill: Velikatta, Mdl: Kondapak, Dist: Siddipet, Telangana – 502277
JUNE- 2025
 SRIKRUPA INSTITUTE OF PHARMACEUTICAL SCIENCES
(Affiliated to Osmania University & Approved by AICTE/PCI, New Delhi)

Vill: Velikatta, Mdl: Kondapak, Siddipet, Telangana. PIN:502277

CERTIFICATE

This is to certify that the Project report entitled "SYNTHESIS AND ANTIBACTERIAL

ACTIVITY OF SUBSTITUTED 1,3-THIAZINE DERIVATIVES" submitted by Mr. A.

Abhinav Reddy (636221881001), Ms. T. Akhila(636221881009), Mr. J. Madhu

(636221881042), Mr. Soumaybrata Saha (636221881073), Ms. B. Sneha (636221881085),

Mr. B. Srinivas (636221881090) for the award of degree of Bachelor of Pharmacy to the

Osmania University, Hyderabad, Telangana for the academic year 2024-2025 under the

guidance of Dr. B. RAVINDAR, M. Pharm., Ph. D, Department of Pharmaceutical Chemistry,

Srikrupa Institute of Pharmaceutical Sciences, Velikatta, Siddipet.

Signature of supervisor
Dr. B. RAVINDAR M. Pharm., Ph. D
Associate Professor
Department of Pharmaceutical Chemistry

Place: Siddipet

Date:

Signature of Principal
 ACKNOWLEDGEMENT

Without the grace of the almighty and sincere hard work, the presentation of this dissertation
would not have been possible.

I would like to express my sincere gratitude to:

My esteemed research guide Dr. B. Ravindar, [Link]., Ph. D Professor & Department of
Pharmaceutical Chemistry, Srikrupa Institute of Pharmaceutical Sciences, His tolerance in
every step, keen interest, regular observations, kind co-operation, boundless enthusiasm, and
affectionate encouragement, simulative and constractive criticism throughout this work has
brought up this project work into this shape.
The Chairman, Prof. V. Malla Reddy, Ph.D., D.H.M.S., FRSC an Emeritus Professor of
AICTE, & to the Management, Srikrupa Instinite of Pharmaceutical Sciences, for providing
support.

Our honored Principal Dr. Manjunath S.Y. [Link]., Ph.D., Professor & Head,
Department of Pharmaceutical Chemistry, Srikrupa Institute of Pharmaceutical Sciences,
for his valuable help and providing me the necessary laboratory facilities to carry out this
work.
All other teaching, non-teaching staff of Srikrupa Institute of Pharmaceutical Sciences for
all that they meant to us during the crucial times of the completion and making this project
possible.
Last but not the least special thanks to the management of the Srikrupa Institute of
Pharmaceutical Sciences, for providing such facilities for making success of my project.

I offer my regards to all of those who supported me in any respect during the completion of
the project & extend my sincere thanks to my well wishers. I am graceful to get this golden
opportunity to express my sincere thanks to all those who have directly or indirectly extended
their helping hands towards me for successful completion of this project. I extend my sincere
thanks to one and all.

A. Abhinav Reddy, T. Akhila, J. Madhu,

Soumaybrata Saha, B. Sneha, [Link].
ABBREVIATIONS

TLC - Thin layer chromatography

UV - Ultra Violet spectroscopy

IR - Infrared Spectroscopy

MS - Mass spectroscopy

NMR - Nuclear Magnetic Resonance

M.P - Melting Point

DMF - Dimethyl formamide

DMSO - Dimethyl sulfoxide

MeOH - Methanol

EtOH - Ethanol

% - Percentage
gm - Gram
mm - Millimeter
ml - Milliliter
mol - Molar ratio
mg - Milligram
Gram (+ve) - Gram positive bacteria

Gram (-ve) - Gram negative bacteria

Rf - Retention factor
MIC - Minimum Inhibitory concentration.
ABSTRACT

The synthesis, spectral analysis and biological evaluation of some novel thiazine derivatives

with thiourea have been carried out from various chalcones . The synthesized compounds

have been characterized by IR and 1 HNMR for structure assignment. The antibacterial

activities of these compounds were studied.

KEYWORDS : chalcones, Thiazines, Antibacterial activity.

 TABLE OF CONTENTS

[Link] TITLE [Link]

01. INTRODUCTION 1

02. REVIEW OF LITERATURE 6

03. AIM AND OBJECTIVES 8

04. EXPERIMENTAL WORK 9

05. SCHEME OF WORK 10

06. PHYSICOCHEMICAL PARAMETERS OF 12

THE DERIVATIVES

07. SPECTRAL DATA 14

08. BIOLOGICAL EVALUATION 21

09. RESULT AND DISCUSSION 29

10. SUMMARY AND CONCLUSION 29

11. REFERENCES 30
Chapter I Introduction

Chapter-I
Introduction

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Chapter I Introduction

INTRODUCTION
CHEMISTRY
cinal chemistry is a discipline at the intersection of chemistry and pharmacology
involved with designing, synthesizing and developing pharmaceutical drugs. Medicinal
chemistry involves the identification, synthesis and development of new chemical entities
suitable for therapeutic use. It also includes the study of existing drugs, their biological
properties, and their quantitative structure-activity relationships (QSAR)1. Pharmaceutical
chemistry is focused on quality aspects of medicines and aims to assure fitness for the
purpose of medicinal products.
Compounds used as medicines are overwhelmingly organic compounds including
small organic molecules and biopolymers. However, inorganic compounds and metal-
containing compounds have been found to be useful as drugs. For example, the cis-platin
series of platinum-containing complexes have found use as anti-cancer agents2.
Medicinal chemistry is a highly interdisciplinary science combining organic chemistry with
biochemistry, computational chemistry, pharmacology, pharmacognosy, molecular biology,
statistics, and physical chemistry.
The development of drug resistance has limited the successful application of many antibiotics
and therefore, this phenomenon has graves consequences to health. The design of potential reversers
of microbial resistance has thus become a desirable goal in the field of medical chemistry.

A large number of medical compounds which have been discovered belong to a major
class of heterocyclics containing nitrogen and sulphur. The versatile synthetic applicability
and biological activity of these heterocyclics has helped the medicinal chemist to plan,
organize and implement new approaches towards the discovery of novel drugs.

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Chapter I Introduction

Chalcones :
Chalcones and their analogues having an α,β- unsaturated carbonyl system are very versatile
substrates for the evaluation of various reactions and physiologically active compounds3

The reaction of thiourea with α,β-unsaturated ketones results in 1,3 thiazine. It has being well
focused that the presence of 4-phenyl substituted moieties is an important structural feature
also 2-substituted imino group present in thiazine ring , and the resulting molecule would
exhibit promising biological activities.
Chalcones are prepared by condensing aryl ketones with aromatic aldehydes in presence of
suitable condensing agents. They undergo a variety of chemical reactions and found useful in
synthesis of variety of heterocyclic compounds.
Chlacones have been used as intermediates for the preparation of compounds having
therapeutic value, Literature review reveals that chalcone derivatives exhibit diverse
pharmacological activities such as potential cytotoxic agents, antimicrobial agents, anti-viral,
anti-inflammatory, anesthetics, mydriatrics 4 etc.
Based on the above observation it is worthwhile to prepare newer compounds for their
antimicrobial and anti-inflammatory activities. In the view of the varied biological and
pharmacological application, we synthesized some new heterocyclic derivatives of
chalcones5.
Thiazines are a group of 6 membered hetero cyclic compounds which contains

nitrogen and sulphur as hetero atoms in the aromatic ring.6

[Link] - C4H5NS

[Link] - 99.15 g mol- State

- light yellow crystals

Melting point - 1830C

Boiling point - 2350C

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Chapter I Introduction

Specific Gravity - 1.34

Solubility - Soluble in EtOH

There are three types of basic thiazines introduced. They are,

1,2-Thiazines

N
1,3-Thiazines
S

1,4-Thiazine

Thiazines are an important class of heterocyclics compounds belong studied in recent

years and reported to possess wide spectrum of biological activities. Moreover thiazine
nucleus is a pharmacophore of cephalosporin which occupies a very prominent place in the
field of antibiotics.7

Therefore the emphasis for future research will be the discovery of novel
antibacterial and anticonvulsant agents.

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Chapter I Introduction

ACTIVITY OF THAIZINES
Thiazines are very useful units in the fields of medicinal and pharmaceutical
chemistry and have been reported to exhibit variety of biological activities.8-12

In view of the observations and in continuation of work on biologically active

thiazines and their increasing importance in pharmaceutical and biological field, it was

considered of interest to synthesize some novel derivatives and to evaluate their

biological activities.

Thiazines known to exhibit various biological activities like

• Ca+2 antagonists,
• Blood platelet aggregation inhibitors
• Antibiotics
• Antipsychotic
• Anti cancer
• Anti inflammatory
• Anti oxidant
• Analgesic
• Antimicrobial and Anti-hypertensive agents.

THIAZINE DERIVATIVES

Thiazine derivatives possess versatile type of applications and biological activities13.

Beginning with the electrochemical adsorption studies on alloys for corrosion inhibition,

involvement as electrophilic asymmetric fluorination agents, stabilizers in rubber

vulcanization, fading prevention agents to improve colour image stability and light-fastness,

these derivatives cover divergent biomedicalaspects, including treatment of

immunodeficiency

conditions like asthmatic therapy, hyaluronidase inhibition11, anthelmintic activity, propanolol

comparable β–sympatholytic activityand serving as cytostatic agents.

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Chapter I Introduction

Various 1, 3-thiazines are an interesting class of heterocyclic compounds being studied

in recent years and they are reported to possess a wide spectrum of biological
[Link] naphthalene containing 1,3-thiazines. And 1,3-thiazines derived from
nitrochalcones’ exhibited anticonvulsant and antibacterial activities.14

N-formyl-2-nitromethylene-1,3- thiazine and 5-teer, butyl-2-[4-ethynylphecl]-4H-

1,3thiazines were reported as insecticides.15

Thiazine derivatives serve as raw material for the syntheses of a wide variety of

molecules of high molecular mass like substituted benzothiazepines, play role during organ

operations and transplantation, reduce gastrointestinal toxicity, activate metabolism, improve

hearing in various formulations, antithrombotic,16 hypothermic and antihypoxic activities, act

as histamine receptor [Link] find their use as matrix metalloprotease inhibitors17 and

prodrugs for the treatment of joint disease, cancer metastasis, inflammation and periodontitis.
18

19.
The 1,4-disubstituted benzo-fused urea derivatives act as cytokine inhibitors

In short a large number of applications and biological activities have been shown by the
thiazine derivatives.

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 Chapter-II
Review of Literature

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Chapter II Review of Literature

Review of Literature
1. V.N. PATOLIA et al, reported antimicrobial activity of 10N-{[(Aryl)-amino]-methyl}-
3methoxy-10,10a-dihyro-4a-H-phenothiazine-9-carboxylic acid.
R
NH
COOH
N

S OCH3

R=ARYL
2. JALAL [Link] et al , reported inhibitory activity against IGROVI(ovarian cancer) cell
line of tetrahydro Pyrido[3’,2’:4,5]thieno[2,3-b][1,4]thiazine-8-carboxylic acids.
O
H
O N COOH

R S S N

3. [Link] et al, reported antibacterial activity of cloro-substituted-1,3-thiazines.

C l

C H 3

(C H 2 )5
C l
O H P h N S

N P h

4. WIESLAW MALINKA et al, reported antibacterial activity of pyrido[3,2-e]-1,2-thiazines.

CH3 OH R

O
N
H 3C N S R'
O2

R=ARYLR1=H, CH3

5. SHIKHA GUPTA et al, reported antioxidant activity of 4H-1,4-benzothiazines.

H
H 3 C N R 1

S
C R 2

C H 3
O

R1=CH3R2=CF3

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Chapter II Review of Literature

6. VIJAY [Link] et al, reported anti inflammatory , analgesic and ulcerogenic

activity of 1,3-thiazine derivative.
R 1

N S

N H 2

H N

H N R

2-Substituted guanidine-4-(2’-amino-5’-substituted phenyl) mercapto-6-phenyl-1,3thiazine

7. [Link] et al, reported antifungal, antibacterial, anti-inflammatory activity of

thiazine derivatives19
NH 2

R N

R 2
R'

R=H, OCH3 R=H, Cl , NO2 R1=C6H5

8. NORRIE PEARCE et al , synthesized ascidithiazone

Ascidiathiazone
9. SERGEY V .RYABUKHIN et al, synthesized pyrimido thiazine derivatives

Hexahydro-5Hpyrimido [5,4-e][1,3]thiazin-5-ones

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Chapter II Review of Literature

AIM AND OBJECTIVES

following are the main specific aims and objectives of the present research. (a)
Synthesis of some new 2-imino, amino and isatin containing thiazine
derivatives.
(b) Physical characterization of newly synthesized compounds.
(c) To supervise the purity and progress of the reaction by TLC. (d) To purify the
synthesized compounds by recryatallization technique using suitable solvents.
(e) To characterize the newly synthesized compounds on the basis of
Infrared, proton NMR, 13 C nuclear magnetic resonance and mass spectral
data and elemental analysis.

PLAN OF WORK

 Review of literature on thiazine and its derivatives

 Synthesis of chalcones
 Synthesis of substituted thiazine derivatives
 Physical characterization of synthesized compounds
 Spectral analysis of synthesized compounds using IR and 1HNMR spectroscopy
 Biological screening of synthesized compounds by anti bacterial activity

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 Chapter-III

Materials &Methods

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Chapter III Materials & Methods

EXPERIMENTAL WORK:

SYNTHETIC METHOD GENERAL PROCEDURE:

A) Synthesis of chalcones(1a-1f)

1a) An equimolar quantities of Benzaldehyde(0.01mol) and acetylacetone(0.01mol) were

dissolved in minimum amount of absolute ethanol (15ml).Sodiumhydroxide
solution(0.02mol) was added slowly and the mixture stirred for 2hrs until the entire mixture
becomes very [Link] the mixture was poured slowly in to 250ml of ice cold water with
constant stirring and kept in refregirator for [Link] mass obtained was filtered, washed
and recrystalized from ethanol.

Melting point : 180-1860c

Percentage Yield : 88%

B) Synthesis of Thiazine derivatives(S1-S6):

1a) A mixture of Benzilidine acetyl acetone(0.01mol) and thiourea(0.01mol) were dissolved

in ethanolic potassium hydroxide(15ml) and the mixture was kept for condensation about 14-
[Link] mixture was then poured in to 200ml of ice-cold water and stirring was continued
for about 1hr and kept in refrigerator for [Link] mass obtained was filtered, washed with
water and recrystalized with [Link] completion of reaction was monitored by TLC.

Melting point :120-1220c

Percentage Yield : 74%

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Chapter III Materials & Methods

SCHEME OF WORK
Aromatic aldehyde with an aromatic substituted ketone

R=R1 = Substituted aryl, hetero aryl/ alkyl groups

Table –I

VARIOUS AROMATIC ALDEHYDES USED IN THE SYNTHESIS

[Link]. Name of the Aromatic aldehyde Structure ‘Ar’

1.
Benzaldehyde

2.
P-DimethylaminoBenzaldehyde

3.
4-Chloro Benzaldehyde

4.

3,4-Dimethoxy Benzaldehyde

5.
3,4,5-TrimethoxyBenzaldehyde

6. 4-Flouro Benzaldehyde

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Chapter III Materials & Methods

Table-II
LIST OF COMPOUNDS SYNTHESIZED

[Link].
COMPOUND NAME STRUCTURE OF COMPOUNDS

S1
Benzaldehyde

S2 P-
DimethylaminoBenzaldehyde

S3
4-Chloro Benzaldehyde

S4
3,4-Dimethoxy Benzaldehyde

S5
3,4,5-TrimethoxyBenzaldehyde

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Chapter III Materials & Methods

PHYSICOCHEMICAL PARAMETERS
Thiazine derivatives synthesized were characterized by the following experimental
methods:

 Melting point: of the synthesized compounds were taken in open capillary tubes and was
uncorrected.
 Thin layer chromatography was performed using plates coated silica gel of 0.25 mm
thickness. Eluents used were chloroform:benzene(3:7).Spots were visualized through the iodine
chamber. Rf values of the newly synthesized compounds were calculated by the following
formula.
Rf = Distance travelled by the solute

Distance travelled by the solvent

 Solubility: At room temperature solubility of a newly synthesized compound were determined

by various organic solvents.

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Chapter III Materials & Methods

Table – IV

PHYSICOCHEMICAL PARAMETERS

Percentag Melting
S.N Compou Molecular
Structures (R) e Yield Point Rf value
O nds Formula
(%) (0C)

1. S1 C12H13N2SO 74% 120- 0.54

1220C

2. S2 C12H12N2SFO 61% 180- 0.56

1820C

3. S3 C12H12N2SO 67% 150- 0.81

CL 1520C

64% 87-890C 0.91

4. S4 C15H19N2O4S

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Chapter III Materials & Methods

5. S5 C14H17N2O3S 102-
1040C
70% 0.62

Table-V SOLUBILITY OF SYNTHESIZED COMPOUNDS

[Link]. COMPOUNDS SOLUBILITY

1 S1 EtOH,DMSO,Acetone,DMF

2 S2 EtOH,DMSO,DMF,Acetone

3 S3 MeOH,Acetone,DMF,DMSO,EtOH

4 S4 EtOH,DMSO,DMF

5 S5 EtOH,DMSO,DMF,MeOH

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Chapter III Materials & Methods

SPECTRAL STUDIES

The objective of the spectral studies is to confirm the chemical structures of the synthesized
compounds, and the various functional groups in the final compounds.

The Infrared spectroscopy was performed with KBr on perkin FT-IR [Link] IR
spectrum data of the synthesized compounds were summarized in Table VII.
1
HNMR was performed for the compounds S2 and S4 .The 1HNMR data spectral
data of the synthesized compounds were summarize.

Table-VII
IR Spectral Data (KBr discs)

[Link] Compounds Structures of Compo Vmax(cm-1)

unds
(Imine)3395,(CyclicN-H)3345
S1
1 (C=C)1652,(C-N)1192,(C-H)methyl
2927,(C=N)1552,(C=O)1661

(Imine)3340,(CyclicNH)3411,(C=C)1603,
2
S2 (C-N)1225,(C-H)2927,
(C=N)1571,(C=O)1662,(C-f)1096

(Imine)3398,(Cyclic N-H)2925,
(C=C)1614 , (C-H)2925,
3 S3
(C=N)1563,(C=O)1655,(C-Cl)1013

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Chapter III Materials & Methods

(Imine)3373, (Cyclic N-H)3195,

4 (C=C)1520,(C-N)945,(C=N)1631, (C-
S4
H)2885,(C=O)1636(C-O-C)1164

(Imine)3346, (Cyclic N-H)3204,

5 (C=C)1558,(C-N)1140,(C=N)1631, (C-
S5
H)2934,(C=O)1560,(C-O-C)1259 , (C-
H)2934

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Chapter III Materials & Methods

FTIR Spectrum of S1

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Chapter III Materials & Methods

FTIR Spectrum of S2

FTIR Spectrum of S3

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Chapter III Materials & Methods

Table- VIII
HNMR Spectral Data

[Link] Compounds Structure of Compounds HNMR(DMSO) δ in PPM

1 S2
2. 54(s,1H, CH),
3. 56(s,3H,CH3),
3.58(s,2H,
CH2), 3.74(s,6H, N(CH3)2),
6.51(s,1H, NH),6.69-6.683(m,
5H, Ar H)

2 S4 2.96(s,1H, CH),
2.73(s,3H,CH3), 3.04(s,2H,
CH2), 3.44(s,6H, N(CH3)2),
6. 75(s, 1H, NH), 7.51-
7. 64(m, 4H, Ar H)

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Chapter III Materials & Methods

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Chapter III Materials & Methods

BIOLOGICAL EVALUTION
The compounds were specifically tested for the antibacterial activity by the serial dilution
method (MIC method) and zone of inhibition method, and anticonvulsant activity by any one
off the method, Chemical (Pentaline tetrazolium-PTZ), or by maximal electric shock (MES)
method.23

Antimicrobial Evaluation24

The Various methods have been used to evaluate the antimicrobial activity of the drugs
with their own advantage and limitations. The techniques involved in evaluation are:

 Agar streak dilution method

 Serial dilution method
 Agar diffusion method
Cup plate method
Paper cylinder method
Disc diffusion method
 Turbid metric method
The antibacterial activities of the compounds will be studied by using cup Plate method.
Media: Nutrient agar will be used as the media for the study.

Nutrient agar composition:

Table -IX
Ingredients Gram/Liter

Peptic Digest of Animal Tissue 5.00

Beef Extract 3.00

Sodium Chloride 5.00

Agar 15.00

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Chapter III Materials & Methods

ANTIBACTERIAL ACTIVITY
In present study the following bacteria were used.25-30

A. Escherichia coli (Gram -ve)

B. Pseudomonas aeruginosa (Gram-ve)
C. Klebsiella pneumoniae (Gram+ve)
D. Staphylococcus aureus (Gram+ve)

In present study the cup-plate method were used to evaluate the antimicrobial activity in
vitro of the synthesized compounds. This method were used for determining the selective
effectives of the anti bacterial activity. The standard antibiotic selected for study of the
antibacterial activity is streptomycin.

DISK DIFFUSION METHOD USING NUTRIENT AGAR

Materials Required:
 Nutrient agar, growth culture in 18-24hrs.
 Sterile Petri dishes
 Sterile pipettes
 Sterile cotton swabs
 Sterile watt man filter paper disks
 Sterile test tubes containing the solution of the test compounds in desired concentrations.

Preparation of nutrient agar:

The definite volumes of peptone (0.6%), yeast extract (0.15%), dipotassium
dihydrogen phosphate (0.36%) and potassium dihydrogen phosphate (0.13%) will be
dissolved in distilled water and pH will be adjusted to 7.2. this solution will be sterilized by
autoclaving at 15 p.s.i. for 20 minutes.

Preparation of sub-culture:
One day prior to the testing, inoculation of the above bacterial cultures was made the nutrient agar
and incubated at 370C for 18-24hrs.
Preparation of test solution31-35 :
Each test compound (10mg/ml) was dissolved in dimethyl sulfoxide (10ml)to give a 1000µg/ml

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Chapter III Materials & Methods

Method of testing:
Base layer was obtained by pouring about 10-15ml of the base layer medium in to each
sterilized petri dishes and allowed to attain at room temperature .The overnight grown
subculture was taken in to definite volume of inoculated ,then with the help of cotton swab
the organisms were streaked the entire agar surface horizontally,vertically,and around the
outer edge of the plate to ensure a heavy growth over the entire [Link] all culture
plates to dry for about 5minutes.
Disks are prepared and dipped in to various concentrations of test
solutions for 10min. with the help of forceps, disks are placed on the agar medium in petri
plates. Likewise disks of different concentrations are placed on agar medium .The disks are
heated in Bunsen burner flame to make it sterile. These plates were incubated all the plate
cultures in inverted position for 24hrsat 370C and observed for antimicrobial activity36-40.
Streptomycin was used as standard drug and the solvent control (DMSO/DMF) was kept
separately.
After 24hrs, the diameter of zone of inhibition and minimum inhibitory
concentration (MICs)were measured in mm for each organism. Zone of inhibition were
determined for all the four compounds, the results in the form of percent inhibition will
summarized in the form of tables.

Table-IX Antibacterial activity against Escherichia coli

Concentration Zone of inhibition

in µg/ml in(mm)

S1 S2 S3 S4 S5 S6 std

200 12 14 12 15 16 12 20

300 18 17 13 17 17 13 20

400 19 19 17 19 19 16 20

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Chapter III Materials & Methods

Streptomycin 100µg/ml used as a standard drug

Chart Title
20
18
16
14
12
10
8
6
4
2
0
S1 S2 S3 S4 S5 S6 std

200 300 400

Table- X
Antibacterial activity against staphylococcus aureus

Concentration Zone of inhibition (mm)

in µg/ml

S1 S2 S3 S4 S5 S6 Std

13 12 12 14 10 15 24
200
14 16 13 17 14 18 24
300
17 18 15 19 17 19 24
400

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Chapter III Materials & Methods

Streptomycin 100µg/ml used as standard drug

20
18
16
14
12 200
10 300
8
400
6
4
2
0
1 2 3 4 5 6

Table-XI
Antibacterial activity against Pseudomonas aeruginosa

Concentration Zone of inhibition in (mm)

in µg/ml
S1 S2 S3 S4 S5 S6 Std
13 12 16 16 15 12 26
200
15 14 18 17 16 17 26
300
17 18 19 20 19 19 26
400

Streptomycin 100µg/ml used as standard drug

30

20
200
10 300

0
1 2 3 4 5 6

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Chapter III Materials & Methods

Table-XII
Antibacterial Activity against Bacillus subtilis

Zone of inhibition in (mm)

Concentration
in µg/ml
S1 S2 S3 S4 S5 S6 Std
15 10 14 13 12 17 25
200

17 12 17 14 13 19 25
300

20 16 19 16 15 21 25
400

Streptomycin 100µg/ml used as standard drug

50
45
40
35
30 17
25 20
20
400
15
10
5
0
1 2 3 4 5 6

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Chapter III Materials & Methods

100µg/ml for test compounds

200µg/ml for test compounds

Table-XIII

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Chapter III Materials & Methods

Antibacterial activity data for MIC

Zone of inhibition in (mm)

[Link] Compounds [Link] [Link] [Link] [Link]

1 S1 14 16 14 12

2 S2 17 13 12 15

3 S3 13 12 17 14

4 S4 17 15 13 16

5 S5 14 17 15 12

6 S6 16 13 14 13

7 Std 24 22 25 21

25
S1
20
S2
15 S3
S4
10 S5
S6
5 Std

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 Chapter-IV
Results & Discussion

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Chapter IV Results & Discussion

RESULTS AND DISCUSSION

In the present work 4 different thiazine derivatives were synthesized.

Melting point determination, Thin Layer Chromatography ,and IR spectral analysis was
1
characterized for all synthesized compounds. HNMR was characterized for the compound
S2 and S4.
Melting point of all synthesized compounds were found to be in the range of 87-1820C was
determined by using open capillary tube method and uncorrected.
Thin Layer Chromatography was performed for all the synthesized compounds and R f
values was calculated and tabulated in Table [Link] of the drugs was studied by
various solvents. The infrared spectroscopy was performed with KBr on Perkin FT-IR
instrument. The IR spectrum data of the synthesized compounds were summarized in table
[Link].
1
HNMR spectral data for the compounds S2 and S4 were summarized in Table [Link].
All the synthesized compounds were subjected to biological evaluation for antibacterial
activity. The antibacterial activity showed that all synthesized compounds were moderately
active against the bacterial strains.

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 Chapter-V
Conclusions

Srikrupa Institute of Pharmaceutical Sciences Page 34

Chapter V Conclusions

SUMMERY AND CONCLUSION

In the present study, some novel thiazine derivatives have been synthesized and screened for their

biological activity.

• The synthesized compounds were characterized by IR and 1HNMR spectral data. All the

synthesized compounds showed characterized absorption peaks in IR and 1HNMR.

• The newly synthesized compounds were evaluated for their antibacterial activity. The

activity suggests that novel thiazine derivatives showed moderate antibacterial activity.

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 Chapter-VI
References

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Chapter VI References

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