Your report must include the following:
a)Title Page. Include the title of your lab, your
names, the date you submitted your report, your
teacher’s name (Ms. John), course code (SBI…),
and the total # of pages.
b)Each section should include a proper heading,
bolded and underlined.
--DELETE THIS PAGE IN YOUR FINAL COPY—
Anything in the other pages that are bolded in yel-
low…delete
Resources to help you complete an APA style title
page:
[Link]
format/
Introduction:
In one to two paragraphs, explain the difference between the types of macromolecules you
learned in Chapter 1. Explain why macromolecules and osmosis are important to our physiology.
Purpose:
In 1-2 sentences, explain what would be the purpose of conducting a Biochemistry Identify Nu-
trient Lab experiment?
Method: Complete this section using Gizmo
Introduction: Most food is composed of three types of molecules: carbohydrates, pro-
teins, and lipids.
• Carbohydrates such as starches and sugars are major source of energy. Simple
sugars are found in sweets and fruits. Starches are found in potatoes, cereal,
pasta, flour, and other plant products.
• Proteins are used in body structures such as muscles, skin, and hair. Rich
sources of proteins include meats, dairy products, and beans.
• Lipids (fats and oils) are used for energy, insulation, and as an essential building
block of cells. Meats, dairy products, and oily plants such as olives are rich in
lipids.
Question: How do you test for carbohydrates, proteins, and lipids?
1. Test: Under the Benedict test, click Test. Is the Benedict test positive for sample A?
_____
Recall that orange is a positive test for monosaccharides (and some disaccharides).
2. Test: The Lugol test uses iodine to test for starch, a polysaccharide (complex car-
bohydrate). Iodine turns dark purple in the presence of starch.
Under Lugol test, click Test. Does sample A contain starch? _____
3. Test: The Biuret test uses a solution of potassium hydroxide (KOH) and copper sul-
fate (CuSO4) to test for protein. The Biuret solution turns purple when proteins are
present.
Under Biuret test, click Test. Does sample A contain proteins? _____
4. Test: The Sudan Red test uses a fat-soluble dye, Sudan Red, to indicate the pres-
ence of lipids. When lipids are present, the dye will be absorbed into the lipids, and
will appear as concentrated spots of color in the test tube. (No spots indicates that
lipids are not present.)
Under Sudan Red test, click Test. Does sample A contain lipids? _____
5. What nutrients does sample A contain? _______________________________
6. What kind of food is sample A most likely to be? (Circle your choice)
A. Apple juice B. Baked beans C. Oatmeal D. Scrambled eggs
Collect Data: Complete this section using Gizmo
What nutrients does each food sample contain?
1. Use the four available tests to find the nutritional content of samples E, G, and M.
(Sample A has been done for you as an example.) Record results on the table be-
low.
Carbohydrate Protein Lipids Test results – are these nutrients
Tests Test Test present?
Mono-
Food Benedict Lugol Biuret Sudan
saccha- Starches Proteins Lipids
sample test test test Red test
rides
A + – – – Yes No No No
E
M
Analysis: Answers must be in full sentences (do not start the question with yes, no, and, but,
maybe etc)
Look at the results for samples A, E, G, and M.
A. Is sample E most likely to be steak, bread, or butter? Justify your answer.
B. Is sample G most likely to be table sugar, pasta, or olive oil? Justify your an-
swer.
C. Is sample M most likely to be chicken, rice, a mango, or butter? Justify your
answer.
Discussion Questions: Answers must be in full sentences (do not start the question with yes,
no, and, but, maybe etc)
1. A student takes a sample of protein, adds Biuret Reagent and then heats the test tube. The stu-
dent notices a colour change from violet to blue. Explain why heating caused this colour change.
2. You test the following food samples during a Biochemistry lab for Identifying Nutrients, using
the data chart match the food item with the sample letter. For example, Apple Juice is Sample C.
Here are the food samples:
Salmon Oatmeal Peach
Whole Milk Chocolate Chip Cookie Margarine
3. Physicians often want to know the glucose and lipid levels in a patient’s blood and whether
proteins are present in a patient’s urine. Research and discuss how this information is important
in determining the status of an individual’s health.
4. In general, a balanced diet contains relatively even amount of carbohydrates, proteins, and
lipids. Too much sugar is unhealthy. Fruits and vegetables are important course of vitamins and
minerals.
Do you consider your diet balanced and healthy? Why or why not?