EXPERIMENT NO.
15
2 The eyepiece graticule scale in your microscope may be used to measure the actual length of the
layers of tissue or cells, if the scale has been calibrated against a stage micrometer.
However, to help draw the correct shape and proportion of tissues, as in (a), it is not necessary to
calibrate the eyepiece graticule scale.
K1 and K2 are slides showing stained transverse sections through blood vessels.
(a) Draw large plan diagrams of two different types of blood vessel shown in K1 and K2.
On your diagram, use ruled label lines and labels to describe (annotate) one observable
difference in the layer lining the inside of each vessel.
D
Thick Tunica Media
[5]
9700/35/M/J/14
Fig. 2.1 is a photomicrograph of blood cells taken from a person suffering from cancer affecting the
white blood cells.
Fig. 2.1
(b) Make a large drawing of five whole white blood cells in the area shown on Fig. 2.1.
Use ruled label lines and labels to one cell you have drawn to identify three cell structures
that can also be found in plant cells.
①
⑳ &
⑰ [5]
(c) Fig. 2.2 is a photomicrograph showing blood cells from a healthy person.
magnification x500
Fig. 2.2
(i) To diagnose cancer of the white blood cells a sample of blood is taken and a total blood
cell count is carried out on the sample.
State one difference, other than size, between the blood shown in Fig. 2.1 and that in
Fig. 2.2 that indicates that the person is suffering from cancer which affects the white
blood cells.
Explain the reason for this difference.
Greater number
of white blood cells in
Fig
difference ...........................................................................................................................
2 . .
1
Due to uncontrolled cell divison
explanation ........................................................................................................................
.
.......................................................................................................................................[2]
Fig. 2.3 is a photomicrograph of blood cells from a different species of animal.
T P
13mm 15mm
Q
13 mm
R
14mm
13 m m
magnification x500
Fig. 2.3
(ii) A student measured the longest length of five red blood cells P, Q, R, S and T shown in
Fig. 2.3 and found the mean actual length of the red blood cells.
Use the magnification to calculate the mean actual length, in μm, of the red blood cells
using the five labelled red blood cells in Fig. 2.3.
You may lose marks if you do not show your working or if you do not use appropriate
units. Mean
image length
15mm + Bmm + 14mm + 13mm 13 mm +
-
= 13 .
6 mm
5
13600 um
I 13600
"too
um
27 2
mean actual length ......................................................μm
.
[4]
(d) Prepare the space below so that it is suitable for you to record the observable differences
between the red blood cells in Fig. 2.2 and Fig. 2.3.
Record your observations in the space you have prepared.
Feature T.
Fig 22 2 3
Fig
.
. .
nucleus absent present
Oval
shape of cell
round/irregular
of
number cells
few
more
[3]
[Total: 19]