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RNA Concentration for cDNA Synthesis

The document contains a detailed table of cDNA calculations for RNA extracted from liver and lung tissues, including RNA concentrations and volumes needed for dilutions. It provides specific measurements for various groups and their respective RNA concentrations in ng/μl and μg/μl. The data is organized to facilitate the preparation of cDNA from the RNA samples.

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10 views1 page

RNA Concentration for cDNA Synthesis

The document contains a detailed table of cDNA calculations for RNA extracted from liver and lung tissues, including RNA concentrations and volumes needed for dilutions. It provides specific measurements for various groups and their respective RNA concentrations in ng/μl and μg/μl. The data is organized to facilitate the preparation of cDNA from the RNA samples.

Uploaded by

bmb21030
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

cDNA CALCULATION

Group Tissue RNA (ng/μl) RNA (μg/μl) 1:10 dilution


μl taken up to 1μg ddH2O
B1 Liver 2409.40 2.41 0.24 4.15 0.85
B2 Liver
C1 Liver 3411.50 3.41 0.34 2.93 2.07
C2 Liver 2888.00 2.89 0.29 3.46 1.54
D1 Liver 1885.00 1.89 0.19 5.31 -0.31
D2 Liver 2261.70 2.26 0.23 4.42 0.58
E1 Liver 2683.00 2.68 0.27 3.73 1.27
E2 Liver 6090.00 6.09 0.61 1.64 3.36
F1 Liver 3305.80 3.31 0.33 3.02 1.98
F2 Liver 9565.00 9.57 0.96 1.05 3.95
Group Tissue RNA (ng/μl) RNA (μg/μl) 1:10 dilution
μl taken up to 1μg ddH2O
B1 Lung 8:10 dilution
B2 Lung 379.00 0.38 0.27 3.77 1.23
C1 Lung 484.00 0.48 0.34 2.95 2.05
C2 Lung 719.50 0.72 0.50 1.99 3.01
D1 Lung 336.80 0.34 0.24 4.24 0.76
D2 Lung 20.00 0.02 0.01 71.43 -66.43
E1 Lung 745.00 0.75 0.52 1.92 3.08
E2 Lung 469.80 0.47 0.33 3.04 1.96
F1 Lung 827.00 0.83 0.58 1.73 3.27
F2 Lung 516.60 0.52 0.36 2.77 2.23

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