ir 2 enryrued (Encto + Exo mucrdore “Saul Crecouedbar © ECHNOLOGY : PRINCIPLES PROCESSES Principles of Biotechnology bout ety be coc independenty rom the coding strand of DNA. pee vied pe reee ean, ‘The definition given by EFB is as follows: integration of natural science and ‘ells, parts thereof, and molecular analog andsenices.—> mowenin ooh 11.1 Pamcrtes or BiorecuNoLocyw 4 ; preagnsent Of G Clete ye to inti these Ie ae Sea ee YY en vecrou suactponts gone inte ee ETS To moar cou Peres nau To neta SSlaas hs . ac tena gptse eo sts Crug 7 Bake AEN congue fs x oige- bee Hu0d Hyp nicsican's © ‘Of DNA has become part of a chromo replicate, Ina chromosome there Isa replication, which is res sistance ger EP PIEE frcontering antibiotic resistance. The cutting of DNA Specific’ became possible with the discovery of the s0-cilk its progeny. .2 Toois or Recompmart DNA Tecunotoar © Rose gi we know from the fre = ae A a are ne discussion that genetic engneting ese added_> ale e-cou! DNA pour rm orae ron weanioph iat seu “BooRI comes from | 0%! ed om thenane f 77 ERO NCTE OLE aulges iocernas grosphouialey ondY i sev mmc 0 Mots : a Forerampl * epte same. Forexample, the lowing sequences eads ish ge strands n> 8 dct Se 22 3 5 direction. — © owe — vi | phophowierr om bona Hydrerassor at specie posons}eithin the pee ce mS oR te a nce mci ean a ~y Because OF Bricky ends |e use ganie REV . ‘Action of Restriction eBaing are used in genetic engineering to form : | 40 cunning veo [Link] IA which are composed of DNA frees . EOR(@Qh ONP. — Treennme cutabath DNA the Dia ben ass eat wane CEE a oe : egulant DNA agnts oo 2a anwutoquer icien es ca be Joined ogee, ce enntte ft (veto DN] recon ree vst, ia cot ap gam BEY Gere TEC aed = sree mesaqcephite sp wind mer anacet! eee es = steed Tae nes ‘i 4 cam i eceeree o awuor ot. on orceee j oie aye 5) Mae Be ee (@-9-- --dr-a) = GUE teal 6) Pact 1 eRGCRS ; setets UrGntess source and Recombinant DNA’ VEEFEM OWA OuLs Gur |) ‘Matecule by fait rearatiction | enryme , Reconte: vecr Transcuction canner ee esoauctd Gen cg | NTEEEEIR) = uh nous muenate: { FF eved wastyn- need armani f oy ae ) ening Ho YONA poe @ enter Jenene! eng: GSD iicatine proconorase rentove phorpnare quip euoe! send Is crascsi phat of reoettnane Di ceed \Dteunina ruancy ence cohveurt paint end ro seleny. en by pow, Ap on poty TT: oi To a ‘word iS read in both directions, the gailindromie tn DNA is a/sequent ‘qfbase pairs that reads same on the two strands when orientation!a [sfeh rons apstiens es ice ofDNA when ikedtoihis sequence > Cont moIt/ng aopy This sequenceisalso — NUmben, eet 2020+ @sriice wor s0" ‘ Bice ONE #708™ ~¥ Taentifising and atminating non~ transformant peaaane as feowone cou —_ynind! Mabel! BeoR Cla Hind 11.8 A typal agarose gel 3 Z « S ctont 4 news 119 3 epoca sort 4 Betonfng 9's aires ‘example, you can ligate foreign DNA Figure 11.4 coll clo tetracycline, ‘shot tor pBR322. tite ‘The recombinant plasmids will lose Taree gre ee ee | containing tetracycline. The recombinants will growin ampicilin “OPS res ———— > Non - essential F Pouble Rerandid | Feu euapticarny | = Antibiore easietandeS foay : PRNCIPLS AND PROCESEES by ‘hem Nv Bot the only Way to introduce allen iow that we have lam about the tools fr constructing recombinant pn Jets discuss the processes facitating recombinant DNA technolo @ruans poo :-unle 04 BNR have the ability rich con NOW gront better inderstanding ofthe art ofdelivering genes By path ene ON > anorrex” — | their eukaryotic hosts has generated knowledge to transfors toot of pathogens nt use with other macromolecules such as RNA, proteins, alysaccharides and also lipids, This can be achiewed by treating comprare. done cored ‘he bacterial cels/plant or aiimal tisque with enzymes such as gions Husadan “genence Mantoranimalhost (en ‘guoeyme [Soci cellulase pant ccs} ehtinaseCunsust) ioe sne aenceaL, net BaCtONSS 's Competent Host (For Transformation with ‘You know that genes are located on long molecules of DNA pe pecouit? Recombinant DNA) s Interwined with proteins such as histones, The RNA can be removed by A puaing, acm because ate gouns ait colth ALA Which auc to ret CoMbILTY prt outYo Invi an view ve v 4 ourcide body — Sngidt booty 11.8.2 Cutting of DNA at Specific Locations Restriction enzyme digestions are performed by neu DMA—> annods — molecules with the “ing p Figure 11.6 Polygerase chain reaction (CR): ach cycle has thre steps: i? Senaturaton: ‘Wir Primer annealing, and )-#tension of primers . |\y € % upsrauont proces! eypucssion oF puoduer atest geting puom DF spcrmncinss ANDO PROCESS yoo spownseream Proceess pnour siorsactOis ering Tepe arion. rraveanton | oa Di nerwvaron | y ssicr quontint a rad Goohe Fontstor rearing ee tradi Phe ae os