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Pregnancy Diagnosis Methods in Animals

The document outlines various methods for diagnosing pregnancy in animals, including the Punya Koti Test, PGIMFT, MPA, Protein Binding Test, and Per Rectal Examination. Additional methods mentioned are ultrasonic scanning, vaginal biopsy, and radiography, among others. Each method has specific procedures and indicators for determining pregnancy status.

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Shiva Shivayadav
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15 views1 page

Pregnancy Diagnosis Methods in Animals

The document outlines various methods for diagnosing pregnancy in animals, including the Punya Koti Test, PGIMFT, MPA, Protein Binding Test, and Per Rectal Examination. Additional methods mentioned are ultrasonic scanning, vaginal biopsy, and radiography, among others. Each method has specific procedures and indicators for determining pregnancy status.

Uploaded by

Shiva Shivayadav
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

PREGNANCY DIAGNOSIS METHODS:

PUNYA KOTI TEST:


* Collect 100 ml of urine from the animal to be tested on 19-24 days of AI, add 400 ml of water.
* Sprinkle the diluted urine on food grains (Paddy/Wheat/Greengram etc.) spread over in a pot
for 2-3 days.
* Observe grains after 6 days.
* If grains becomes black and no germination  +ve for Pregnancy.
* If 35-65% grains germinates  -ve for Pregnancy.

PGIMFT (PROSTAGLANDIN INDUCED MILK FLOW TEST):


* Prepare a stock solution by mixing 1 ml Prostaglandin hormone with 19 ml distilled water.
* Inject the stock solution (1 ml for Cows & 2 ml for Buffaloes) into the ear vein.
* If udder is ready for milk let down within 5 minutes +ve for Pregnancy.
* If there is no milk let down  -ve for Pregnancy.
* Care must be taken to remove all the milk from the udder before proceeding for this test.

MPA (MILK PROGESTERONE ASSAY):


* Pregnancy can also be assessed by testing plasma or milk collected from animals after 21-24
days of insemination.
* If the animal is not conceived, the Progesterone hormone will be around 7 mg/ml of milk,
and even lesser in plasma, while the hormone level increases with conception.

PROTEIN BINDING TEST:


* On 24th day of insemination, conception can be known by assessing the progesterone from the
animal’s body hair through protein binding.

PER RECTAL EXAMINATION:


* After 3 months of insemination, the Per rectal examination/palpation of reproductive tract.
(Detailed below)

OTHER METHODS:

ULTRASONIC SCANNING
TRANS-ABDOMINAL ULTRASOUND IN EWES
ASSESSING THE FOETAL ANTIGEN IN THE BLOOD OF PREGNANT ANIMAL
VAGINAL BIOPSY
RADIOGRAPHY (From 70 days of gestation)
ROSETTE INHIBITION TITRE TEST (presence of Early Pregnancy Factor (EPF) in ewes as
early as 24 hours after mating).
PALPATION OF CAUDAL UTERINE ARTERY
PERITONEOSCOPY (17-28th day of insemination – 91% accuracy)

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