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Cell Disruption Methods Overview

The document discusses cell disruption methods including scanning electron micrographs of E. coli and diagrams of prokaryote and fungal cell structures. It also discusses the extraction of cyclosporin A from fungi using liquid extraction and permeabilization of E. coli cells using detergent.

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Abhishek Kumar
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5 views69 pages

Cell Disruption Methods Overview

The document discusses cell disruption methods including scanning electron micrographs of E. coli and diagrams of prokaryote and fungal cell structures. It also discusses the extraction of cyclosporin A from fungi using liquid extraction and permeabilization of E. coli cells using detergent.

Uploaded by

Abhishek Kumar
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd

BAT Assignment On Cell Disruption Methods

BY: Abhishek Kumar Section B Roll no.- 251 2010-2014

Scanning Electron Micrograph of E. coli

Schematic Diagram of A Prokaryote Cell


E

Filamentous Structure of Fungi

May Ly and Argyrios Margaritis. Effect of Temperature on the Extraction Kinetics and Diffusivity of Cyclosporin A in the Fungus Tolypocladium inflatum. Biotechnology and Bioengineering, Vol. 96, No. 5, April 1, 2007. l

Immune suppressive Drug for Organ transplant patients


F

May Ly and Argyrios Margaritis. Effect of Temperature on the Extraction Kinetics and Diffusivity of Cyclosporin A in the Fungus Tolypocladium inflatum. Biotechnology and Bioengineering, Vol. 96, No. 5, April 1, 2007.

Proposed Liquid Extraction Mechanism

May Ly and Argyrios Margaritis. Effect of Temperature on the Extraction Kinetics and Diffusivity of Cyclosporin A in the Fungus Tolypocladium inflatum. Biotechnology and Bioengineering, Vol. 96, No. 5, April 1, 2007.

E. Coli Cell permeabilization using Detergent

DNA leakage out of E. coli due to cell permeabilization

Cell disruption summary


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Cell disruption summary


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