Aim: To determine the purity percentage in the given seed sample
Materials: Chickpea (Cicer arietinum) seeds were used in our study.
Method
The working sample
The purity analysis was done on the working sample collected from local market. The
analysis was made on one working sample. The seed lot is divided in 3 lots of 50 gm each
and tested.
Purity separation
The working sample after weighing is separated into its components viz., pure seed, other
seed crop, weed seed and inert matter.
Pure seed
It includes all botanical varieties of that kind / species. Immature, undersized, shrivelled,
diseased or germinated seeds are also pure seeds. It also includes broken seeds, if the size
is >1/2 of the original size except in leguminacea, and cruciferae where the seed coat
entirely removed are regarded as inert matter.
Other crop seed
It refers to the seeds of crops other than the kind being examined.
Weed Seed
It includes seeds of those species normally recognized as weeds or specified under Seed
Act as a noxious weed.
Inert matter
It includes seed like structures, stem pieces, leaves, sand particles, stone particle.
Method of purity separation
• Three samples of 50 gm each from the seed lot were taken.
• Sample was placed on the work board after sieving and separated into other crop
seeds and inert matter.
• After separation, each portion was weighed and purity percentage was calculated.
• The type of inert matter present was noted.
Calculation
All the four components must be weighed to the required number of decimal places. The
percentages of the components are determined as follows.
Weight of individual component
% of components = _________________________________ X 100
Total weight of all components.
If there is a gain or loss between the weight of the original samples and the sum of all the
components is in excess of one percent, another analysis should be made.
Observations:
Sl No. Particulars Weight (in gm)
Sample I Sample II Sample III
1 Pure Seed
2 Damaged seeds
3 Small Seeds
4 Shrunken Seeds
5 Inert matter (dry plant parts, soil particles etc)
6 Weed Seeds
7 Total weight
8 Pure Seed = total wt. – inert matter
Purity % in samples A B C
Now calculate the Average purity percentage: (A + B + C) / 3