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Instrumentation Amplifier Lab Guide

The document outlines a laboratory exercise focused on the implementation of an instrumentation amplifier using operational amplifiers, emphasizing its importance in amplifying low-level signals in the presence of noise. It details the components required, the construction process, and the calculations for gain and common-mode rejection ratio (CMRR). Additionally, it includes lab work steps, questions for analysis, and report submission guidelines.

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0% found this document useful (0 votes)
14 views9 pages

Instrumentation Amplifier Lab Guide

The document outlines a laboratory exercise focused on the implementation of an instrumentation amplifier using operational amplifiers, emphasizing its importance in amplifying low-level signals in the presence of noise. It details the components required, the construction process, and the calculations for gain and common-mode rejection ratio (CMRR). Additionally, it includes lab work steps, questions for analysis, and report submission guidelines.

Uploaded by

ahmedsamiul95
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

BME 304: Biomedical Instrumentation and Measurements Lab

Lab 04: Implementation of an Instrumentation Amplifier


using Op Amps

Introduction:

An instrumentation amplifier (IA) provides a large amount of gain for very low-level signals,
often in the presence of high noise levels. The major properties of IAs are high gain, large
common-mode rejection ratio (CMRR), and very high input impedance. It is a device that
amplifies the difference between two input signal voltages while rejecting any signals that are
common to both inputs. Instrumentation amplifiers are essential components in many
electronic systems where precise amplification of small differential signals is necessary,
particularly in applications involving sensors, transducers, and data acquisition systems.

Learning Objectives:

At the end of this lab you should be able to:


• Gain a basic understanding of an instrumentation amplifier
• Design and implement the instrumentation amplifier for the acquisition of different
bio-potential signals

Required Components

ID. Component name Qty.


1 LM741 Operational Amplifier 3
2 1 kΩ Resistor 1
3 3.3 kΩ Resistor 1
4 10 kΩ Resistor 5
5 22 kΩ Resistor 1
6 100 kΩ Resistor 1
7 330 kΩ Resistor 1
8 DC supply 1

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BME 304: Biomedical Instrumentation and Measurements Lab

9 DC supply probes 2
10 Function generator 1
11 Alligator clip cables 2
12 Oscilloscope 1
13 Oscilloscope probe 2
14 Connecting wires
Building the Instrumentation amplifier

Typical bio-potential amplifiers have high input impedance and are designed for safety first.
This is due to the fact that the signal amplified is being drawn from a living organism so
precautions must be taken in order to prevent macro and micro shock. Isolation and protection
circuitry are used to limit current through electrodes to safe levels. The output impedance of
the amplifier should be very low to drive any external load with minimal distortion. Again,
due to the small size of the signal, the gain should be large. Typically, again of over 1000 is
implemented in bio-potential amplifier circuits. The amplifiers should have a high common
mode rejection ratio to eliminate large offset signals. Finally, most biopotential amplifiers are
differential. Differential amplifiers are used to make sure that noise from the inputs are not
amplified thus yielding a higher integrity signal. Differential amplifiers with such
characteristics are difficult to find. Thus, combinations of differential amplifiers are used to
construct what is called an instrumentation amplifier.

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BME 304: Biomedical Instrumentation and Measurements Lab

Fig 1: A basic three Op-Amp instrumentation amplifier.

Gain and output expression of the instrumentation amplifier:

2RF
Gain of input stage, G1 = (1+ )
Rgain
R3
Gain of the differential stage, G2 =
R2
The overall gain of the instrumentation amplifier, G=G1* G2
2RF R3
= (1+ )*
Rgain R2
2RF R3
Output, Vout = (1+ )* * (V2-V1)
Rgain R2

The instrumentation amplifier is a circuit configuration that potentially combines the best
features desirable for bio-potential measurements, namely, high differential gain, low
common mode gain, high common mode rejection ratio (CMRR), and high input resistance.
A basic three-op-amp instrumentation amplifier is shown in Fig. 3. There are two stages for

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BME 304: Biomedical Instrumentation and Measurements Lab

the instrumentation amplifier that help make it meet the characteristics of an ideal bio-
potential amplifier. The first stage is the input stage of the amplifier followed by the
differential stage. The front end of the amplifier has two op amps, which consist of two non-
inverting amplifiers that have been coupled together by a common resistor Rgain. The gain of
the first stage is (1 + 2RF/Rgain). The input stage ideally supplies no common mode gain thus
eliminating common mode noise. Finally, the outputs of the input stage are the inputs of the
second stage. The second stage is a conventional differential amplifier with a differential gain
of (R3/R2). This stage has low impedance and provides a high common mode rejection ratio.
The overall gain of the instrumentation amplifier is given below:

2RF R3
G = (1+ R )* R
gain 2

This design results in the desired differential gain distributed over two stages of the amplifier.
It also achieves a very high input resistance as a result of the non-inverting amplifier front end.
It exhibits a very high CMRR as a result of the differential first stage followed by a second-
stage differential amplifier. The CMRR is enhanced by adjusting one of the matching resistors
and by selecting high CMRR op-amps. This instrumentation amplifier is a key design
component universal to many biosensor interfaces and almost all bio-potential instruments.

Texas Instruments supplies such an amplifier for use in the medical industry. Fig.2 shows the
schematic of TI’s most common instrumentation amplifier the INA333. This single chip
allows the user flexibility in adjusting the gain while also reducing circuit design errors. In
our lab, we have a similar Instrumentation amplifier IC INA128. However, in this experiment,
we will build our own instrumentation amplifier circuit.

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BME 304: Biomedical Instrumentation and Measurements Lab

Fig 2: Schematic of INA333.

Lab Work 01
Steps:

1. Connect the circuit as instructed in Figure 3. The pin numbers of the LM741 op-amp are
given in Figure 3.

2. Fix the values of the resistors Rf, R2, R3, and R gain according to the pre-defined gain
mentioned in data table 1. You can record its value using the ohmmeter.

3. For biasing the OP-AMPS, use DC supply.

4. To test the Instrumentation amplifier, we shall use our signal generator.

5. We will observe the desired changes by connecting the Oscilloscope Probe to the Vout
pin.

6. Keep the voltage level of the Inputs low as the amplification of a larger signal will be
clipped due to Low biasing voltage.

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BME 304: Biomedical Instrumentation and Measurements Lab

7. Change the Voltage inputs as instructed. Calculate the gain of this instrumentation
amplifier using the formula provided and compare the observed gains.

8. Find out the theoretical gain of the circuit and compare the theoretical gains to the
observed gains. Comment on the discrepancies in your lab report.

Fig. 3: 741 Op-Amp pin configuration.

Lab Work 02
Common mode rejection ratio (CMRR): CMRR of a differential amplifier (or other device)
is a metric used to quantify the ability of the device to reject common-mode signals, i.e. those
that appear simultaneously and in phase on both inputs. An ideal differential amplifier would
have infinite CMRR, however, this is not achievable in practice. A high CMRR is required
when a differential signal must be amplified in the presence of a possibly large common-mode
input, such as strong electromagnetic interference (EMI).

CMRR is defined as the ratio of the powers of the differential gain over the common-mode
gain, measured in positive decibels (thus using the 20 log rule).

Here Ad is the differential gain and Acm is the common mode gain. As differential gain should
exceed common-mode gain, this will be a positive number. The higher the CMRR, the better.

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BME 304: Biomedical Instrumentation and Measurements Lab

Fig. 4: Instrumentation amplifier circuit with three op-amps.

Unity gain bandwidth/ Gain bandwidth product: Unity-gain bandwidth defines the
frequency at which the gain of an amplifier is equal to 1. In other words, it's the frequency at
which the amplifier's output signal has the same magnitude as the input signal. This
parameter is crucial in understanding the frequency response of an amplifier, especially in
applications where maintaining a constant gain at all frequencies is important.

Steps:

1. You have already constructed the circuit in Figure 4. Now fill up data table 01. Set the
values of the resistances accordingly again.

2. Calculate CMRR according to the equation shown above.

3. Comment on the CMRR values on your lab report.

4. Calculate Unity Gain Bandwidth by increasing the input frequency of both inputs

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BME 304: Biomedical Instrumentation and Measurements Lab

Data Table 01: Testing the Instrumentation amplifier by tuning resistance for the
desired gain

No. Expected Input-1: AC Input-2: AC Observed Unity CMRR


Differential Amplitude Amplitude Gain Gain (dB)
Vout
Gain Bandwidth
(Hz)
1 10 [set 5 mV -5 mV
resistance
such that 10 mV -10 mV
differential
gain is 10]
4 15 [set 5 mV -5 mV
resistance
such that 10 mV -10 mV
differential
gain is 15]
7 20 [set 5 mV -5 mV
resistance
10 mV -10 mV
such that

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BME 304: Biomedical Instrumentation and Measurements Lab

differential
gain is 20]

Lab Questions:
1. Explain why open-loop op-amp configurations are not used in linear applications.

2. What are the main differences between an Op-amp and an instrumentation amplifier?

3. Write down some biomedical applications of instrumentation amplifiers and mention the
reasons for their usage.

4. Can we use INA163 (instrumentation amplifier) for EMG signal acquisition? If not
possible, then give reasons for your answer and suggest an instrumentation amplifier for
this application.

5. What is a good CMRR value for an instrumentation amplifier? Mention how to improve
CMRR for an instrumentation amplifier.

6. Suppose, you want to analyze an EMG signal of amplitude 20 mV. To get visible or
detectable, the output must be at least 500mV. Now, design an instrumentation amplifier
for this purpose [Draw a proper diagram mentioning the values of all components].

Report:

Please hand in your report in the next class. The report should be hand-written (A4 pages,
both sides) except for the codes and figures which have to be printed. The report should
contain the following sections and will be marked out of 10:
a. Objective(s) (1)
b. Attach the Datasheets and Graphs from the experiment and discuss your results (2).
c. Proteus Simulation (3)
d. Answer to the Questions (4)
Please cite all sources you may use to write your report in the References
section. Copying/plagiarism will earn you 0 (zero) marks.

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Common questions

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Maintaining high input impedance in an instrumentation amplifier used for biomedical signal acquisition is crucial because it minimizes the loading effect on the signal source. Bio-potential signals, such as ECG or EMG, are weak and can be significantly affected by the impedance of the measuring device. A high input impedance ensures that almost all of the bio-potential signal voltage is applied across the amplifier's input rather than being dissipated across the source impedance. This reduces signal distortion and preserves signal fidelity, which is essential for accurate biosignal interpretation and diagnosis .

The primary differences between an operational amplifier (op-amp) and an instrumentation amplifier include their specific design and functionality. An op-amp is a general-purpose electronic voltage amplifier with a typically high gain that can be configured to perform a variety of linear functions. In contrast, an instrumentation amplifier is a specialized configuration of multiple op-amps optimized for high differential gain, high input impedance, and high common-mode rejection ratio (CMRR). This makes instrumentation amplifiers particularly suitable for precise amplification of low-level differential signals amidst high noise environments, a feature not inherently robust in standard op-amps .

The gain of an instrumentation amplifier is determined primarily through its configuration of resistors. It is calculated in two main stages: the gain of the input stage (G1) and the gain of the differential stage (G2). The gain of the input stage is given by the formula G1 = (1 + 2RF/Rgain), where RF and Rgain are the feedback and gain-setting resistors, respectively. The differential stage gain is given by G2 = R3/R2, where R3 and R2 are the resistors responsible for setting the differential gain. The overall gain of the amplifier is the product of these two stages: G = G1 * G2. Adjusting the gain involves changing these resistor values to achieve the desired amplification level .

Improving the common-mode rejection ratio (CMRR) in an instrumentation amplifier can be achieved by precisely matching the resistors in the circuit and choosing operational amplifiers with inherently high CMRR values. Adjusting one of the matching resistors to fine-tune performance helps in correcting any imbalances that may diminish CMRR. Furthermore, ensuring the differential stage layout of the amplifier is designed with high precision and selecting high-quality components minimizes parasitic effects that could otherwise introduce common-mode gain, thereby enhancing the overall CMRR .

Potential discrepancies between theoretical and observed gains in instrumentation amplifiers can arise from resistor tolerances, op-amp non-idealities, and parasitic circuit elements. Resistor tolerances affect the precise gain setting, while non-ideal op-amp parameters such as finite bandwidth or offset voltages introduce further inaccuracies. Parasitic capacitance and inductance in the circuit layout may also alter frequency response. To address these issues, it is advisable to use precision resistors with tight tolerances, choose operational amplifiers with specifications matching application requirements, and meticulously design the PCB layout to minimize parasitic effects. Calibration and compensation techniques may also be applied to align observed gain with theoretical calculations .

Designing instrumentation amplifiers with high CMRR presents challenges such as achieving precise resistor matching, high tolerance components, and effective layout to avoid parasitic capacitance. Achieving high CMRR requires minimizing common-mode gain, which means the resistors in the differential stage must be accurately matched, as any mismatches directly affect CMRR. High tolerance precision resistors and stable operational amplifiers are typically employed to ensure performance. Layout considerations also play a crucial role to minimize parasitic elements that could introduce error. Moreover, manufacturers often provide integrated instrumentation amplifier solutions to simplify achieving high CMRR .

A basic three-op-amp instrumentation amplifier design features two main stages: an input stage and a differential stage. The input stage comprises two non-inverting amplifiers coupled by a common resistor, Rgain, which provides initial amplification with high input impedance and no common mode gain. This configuration helps eliminate common mode noise. The differential stage, following the input stage, is a conventional differential amplifier characterized by its gain, which is the ratio of R3 to R2. This stage provides further amplification and enhances the common-mode rejection ratio. The overall gain of the amplifier is given by the expression G = (1 + 2RF/Rgain) * (R3/R2).

The high common-mode rejection ratio (CMRR) is crucial in instrumentation amplifiers, especially for biomedical applications, as it allows the device to distinguish and suppress common-mode signals or noise that are present at both inputs. This is particularly important in environments with strong electromagnetic interference, such as ones where bio-potential signals like EMG or ECG are measured, as these signals are typically very weak compared to the surrounding noise. A high CMRR ensures that the measured signal remains accurate and intact by rejecting identical signals present at both inputs, thus improving the fidelity of the biosignal being amplified .

Open-loop op-amp configurations are generally unsuitable for linear applications due to their extremely high gain which leads to saturation even with small input voltage differences. Because there is no feedback, the op-amp cannot stabilize its gain to a practical level, resulting in output values that either max out or minimize rapidly, eroding linearity and causing distortion. Linear applications require precise control of amplification, which is obtained by incorporating feedback to regulate gain and enhance stability. Therefore, open-loop configurations are more suitable for comparator functions rather than amplification in linear applications .

The unity-gain bandwidth parameter is pivotal in analyzing the frequency response of an instrumentation amplifier because it indicates the frequency at which the amplifier's gain drops to 1. Understanding this bandwidth allows designers to predict how gain will behave at various frequencies, crucial for applications requiring consistent gain across a range of frequencies. As the frequency of the input signal approaches the unity-gain bandwidth, the gain of the amplifier decreases, impacting the amplifier's ability to accurately reproduce the input signal at high frequencies. This parameter helps in designing circuit applications that must maintain precise performance across wide frequency ranges .

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