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Essential Laboratory Science Notes

The document provides comprehensive notes on various topics in laboratory and medical science, including water-soluble and fat-soluble vitamins, electrolytes, and minerals like potassium, zinc, and selenium. It also covers diabetes, its types and risk factors, laboratory techniques such as blood collection methods, and the principles of colorimetry and centrifugation. Additionally, it discusses professional ethics for lab technicians and the properties of mixtures.

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0% found this document useful (0 votes)
6 views27 pages

Essential Laboratory Science Notes

The document provides comprehensive notes on various topics in laboratory and medical science, including water-soluble and fat-soluble vitamins, electrolytes, and minerals like potassium, zinc, and selenium. It also covers diabetes, its types and risk factors, laboratory techniques such as blood collection methods, and the principles of colorimetry and centrifugation. Additionally, it discusses professional ethics for lab technicians and the properties of mixtures.

Uploaded by

shkzishan810
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Complete Laboratory and Medical Science Notes

Water-Soluble Vitamins
Water-soluble vitamins are vitamins that dissolve in water and are not stored in the body. They need to be
consumed regularly through diet. These include Vitamin C and the B-complex vitamins (B1, B2, B3, B5, B6,
B7, B9, B12).
Complete Laboratory and Medical Science Notes

Electrolytes
Electrolytes are minerals in body fluids that carry an electric charge. They help regulate nerve and muscle
function, hydrate the body, balance blood acidity and pressure, and rebuild damaged tissue. Examples
include sodium, potassium, calcium, magnesium, chloride, bicarbonate, and phosphate.
Complete Laboratory and Medical Science Notes

Potassium
Potassium is an essential electrolyte that helps regulate fluid balance, muscle contractions, and nerve
signals. It also helps maintain healthy blood pressure levels.
Complete Laboratory and Medical Science Notes

Zinc
Zinc is a trace mineral important for immune function, wound healing, DNA synthesis, and cell division. It also
supports normal growth and development.
Complete Laboratory and Medical Science Notes

Selenium
Selenium is a trace mineral that functions as an antioxidant, helps regulate thyroid function, and supports the
immune system.
Complete Laboratory and Medical Science Notes

Calcium
Calcium is a major mineral necessary for healthy bones and teeth, muscle contraction, nerve transmission,
and blood clotting.
Complete Laboratory and Medical Science Notes

Creatinine
Creatinine is a waste product formed from muscle metabolism and is excreted by the kidneys. Elevated levels
may indicate kidney dysfunction.
Complete Laboratory and Medical Science Notes

Properties of Mixture
Mixtures are physical combinations of two or more substances. They can be homogeneous or
heterogeneous, do not have a fixed composition, and the components retain their individual properties.
Complete Laboratory and Medical Science Notes

Diabetes and Its Types


Diabetes is a chronic condition where the body cannot properly regulate blood sugar levels. Types include
Type 1 (autoimmune), Type 2 (insulin resistance), and Gestational diabetes (during pregnancy).
Complete Laboratory and Medical Science Notes

Causes and Risk Factors of Diabetes


Risk factors include genetics, obesity, physical inactivity, poor diet, and hormonal changes. Causes vary by
type: Type 1 is autoimmune; Type 2 is linked to lifestyle; Gestational is due to hormonal changes in
pregnancy.
Complete Laboratory and Medical Science Notes

Difference Between Fat-Soluble and Water-Soluble Vitamins


Fat-soluble vitamins (A, D, E, K) dissolve in fat and are stored in the body; water-soluble vitamins
(B-complex, C) dissolve in water and are not stored.
Complete Laboratory and Medical Science Notes

Role of Sugar in the Human Body


Sugar provides energy by being converted into glucose. It fuels cells and supports brain and muscle function
but must be consumed in moderation.
Complete Laboratory and Medical Science Notes

Fat-Soluble Vitamins
These include Vitamins A, D, E, and K. They are stored in body fat and the liver and require fat for
absorption.
Complete Laboratory and Medical Science Notes

Classification of Vitamins
Vitamins are classified into fat-soluble (A, D, E, K) and water-soluble (B-complex, C) based on their solubility
and storage in the body.
Complete Laboratory and Medical Science Notes

Uric Acid and Its Effects


Uric acid is a waste product from purine metabolism. High levels can lead to gout and kidney stones.
Complete Laboratory and Medical Science Notes

Creatinine and Its Test


Creatinine is used to assess kidney function. The test measures its level in the blood and can indicate
impaired kidney function.
Complete Laboratory and Medical Science Notes

GGT Test and Procedure


The GGT (Gamma-glutamyl transferase) test measures enzyme levels in the blood to evaluate liver function.
Blood is drawn from a vein and analyzed in the lab.
Complete Laboratory and Medical Science Notes

Gestational Diabetes and Causes


Gestational diabetes occurs during pregnancy due to hormonal changes that affect insulin use. Risk factors
include obesity and family history.
Complete Laboratory and Medical Science Notes

Centrifuge Principle and Care


Centrifuges separate substances by density using rapid spinning. Care includes balancing tubes, cleaning,
and regular maintenance.
Complete Laboratory and Medical Science Notes

Working and Uses of Colorimetry


Colorimetry measures the concentration of substances based on color intensity. It is used in chemical and
biochemical analysis.
Complete Laboratory and Medical Science Notes

Professional Ethics of Lab Technician


Lab technicians must follow ethics such as accuracy, confidentiality, honesty, and respect for patients and
colleagues.
Complete Laboratory and Medical Science Notes

Types of Cleaners
Cleaners include detergents (remove dirt), disinfectants (kill microbes), solvents (dissolve substances), and
abrasives (scrub surfaces).
Complete Laboratory and Medical Science Notes

Why Artery Method is Used to Collect Blood Sample


Arterial blood samples are used to measure gases like oxygen and carbon dioxide, as they reflect lung
function more accurately than venous samples.
Complete Laboratory and Medical Science Notes

Blood Collection Method - Venipuncture


Venipuncture involves drawing blood from a vein using a sterile needle, commonly from the arm. It is the
most common method used in diagnostics.
Complete Laboratory and Medical Science Notes

Vitamins and Their Classification


Vitamins are organic nutrients classified into fat-soluble (A, D, E, K) and water-soluble (B-complex and C)
based on their solubility.
Complete Laboratory and Medical Science Notes

Laboratory Glasswares
Glasswares like beakers, flasks, pipettes, and test tubes are used for storing, measuring, mixing, and heating
chemicals in laboratories.
Complete Laboratory and Medical Science Notes

Antibody, Antigen, Solution and Solvent


Antibody: Protein produced to neutralize antigens.
Antigen: A substance that triggers an immune response.
Solution: A uniform mixture of solute dissolved in solvent.
Solvent: The component that dissolves the solute in a solution.

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