ESI-MS Triple TOF Requisition Form
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Satyajeet BiswalESI-MS Triple TOF Requisition Form
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Satyajeet BiswalCommon questions
Powered by AIResearchers must acknowledge ATPC in their publications to recognize the facility's contribution and support, as well as to maintain compliance with the terms of service. The specific statement required is: “This research work was carried out in part at the Mass Spectrometry Facility of the Advanced Technology Platform Centre (ATPC) which is managed by the Regional Centre for Biotechnology (RCB), and is funded by the Department of Biotechnology (Grant No. BT.MED-II/ATPC/BSC/01/2010)” .
ATPC recommends users abide by safety rules and sample preparation guidelines to protect personal safety, as well as the safety of the operator and equipment. This includes following proper procedures for handling and preparing samples and acknowledging any liabilities to ensure a safe and controlled laboratory environment .
Following Standard Operating Procedures (SOPs) ensures consistency and reliability in experimental results. In ESI-MS Triple TOF analysis, SOP adherence is crucial for generating high-quality data because it reduces variability and facilitates troubleshooting in case of data discrepancies or unexpected results .
Detailed categorization of sample types and complexity allows for tailored processing and analysis, enabling technicians to prepare the appropriate analytical protocol and resources. It ensures that specific requirements are met depending on whether the sample is a simple molecule, a complex macromolecule, or has undergone specific digestion or fractionation methods, leading to more accurate and meaningful results .
A comprehensive sample information section ensures that detailed data regarding the origin, preparation, and content of samples is available. This supports tailored mass spectrometry protocols, enhances data accuracy, and facilitates troubleshooting. It factors in everything from peptide mass fingerprinting to complex sample digestion methods, ensuring that the analytical framework aligns with the sample's attributes and expected results .
Advance payment is required to secure the analysis services and cover operational costs. The payment must be done through NEFT, with details such as account name (Executive Director, Regional Centre for Biotechnology (ATPC)), account number, bank name, IFSC code, MICR code, GST number, and transaction reference number provided. This process ensures that financial transactions are traceable and organized .
The undertaking section obliges users to follow safety and preparation guidelines, emphasizes their responsibility for correct procedures, and acknowledges that ATPC is not liable for losses or damages beyond its control. This establishes a clear delineation of responsibilities, ensuring users are accountable for their submissions and cooperation with facility protocols .
The key components required in the sample submission form include user name, principal investigator details, contact information, institute/industry name, sample information (such as peptide mass fingerprinting, small synthetic molecule, etc.), complexity of samples, enzyme used, stain used for visualization, and total number of samples. Each component is important to ensure accurate and efficient analysis. User and PI details are necessary for accountability and communication, while sample information specifies the type of analysis and preparation needed. Complexity and enzyme usage indicate the level of processing required, and stain information helps in the visualization of in-gel digestion .
Identifiers for tracking payments include the transaction reference number and total amount paid. These are essential for confirming receipt and correlation of payments with specific services, ensuring financial clarity, and avoiding disputes over unpaid or misallocated amounts .
Providing enzyme details helps in understanding the digestion process used, which is crucial for interpreting mass spectrometry data, while stain information aids in visualization and verification of protein bands during in-gel digestion. This information ensures that the data analyst can adjust parameters accordingly during analysis to account for these factors .