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Equilibrium Calculations for G-6-P Hydrolysis

The document discusses the hydrolysis of glucose-6-phosphate (G-6-P) to glucose (G) and inorganic phosphate (Pi), calculating the equilibrium constant (Keq') based on initial and equilibrium concentrations. It also examines the equilibrium concentrations of fructose-1,6-bisphosphate (F-1,6-BP), dihydroxyacetone phosphate (DHAP), and glyceraldehyde-3-phosphate (G-3-P) when incubated with aldolase. Additionally, it predicts the hydronium ion concentration for a 0.10 M benzoic acid solution using its acid dissociation constant (Ka).

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Rashmi
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7 views4 pages

Equilibrium Calculations for G-6-P Hydrolysis

The document discusses the hydrolysis of glucose-6-phosphate (G-6-P) to glucose (G) and inorganic phosphate (Pi), calculating the equilibrium constant (Keq') based on initial and equilibrium concentrations. It also examines the equilibrium concentrations of fructose-1,6-bisphosphate (F-1,6-BP), dihydroxyacetone phosphate (DHAP), and glyceraldehyde-3-phosphate (G-3-P) when incubated with aldolase. Additionally, it predicts the hydronium ion concentration for a 0.10 M benzoic acid solution using its acid dissociation constant (Ka).

Uploaded by

Rashmi
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Glucose-6-phosphate (G-6-P) is hydrolyzed enzymatically (pH = 7, 25°C) to give glucose

(G) and inorganic phosphate (Pi). The concentration of G-6-P was 0.1 M to start. At
equilibrium, only 0.05% of the original G-6-P remained. Calculate the Keq' for the hydrolysis
reaction

G-6-P G + Pi
I 0.1M or Keg

µ £É%o;%% A
-00005
.

✗ 0.1

.IM/keg=(0.o9qqs)2CG-GPJ--5x,o- s-mX--
@ E [G- 6- B- 0.057×0

0.1-5×10-5
¥-5 ✗ €0.09995m
200
=

This study source was downloaded by 100000870842035 from [Link] on 04-29-2025 09:08:33 GMT -05:00
What are the equilibrium concentrations of fructose-1,6-bisphosphate (F-1,6-BP),
dihydroxyacetone phosphate (DHAP), and glyceraldehyde-3-phosphate (G-3-P) when 1mM
fructose-1,6-bisphosphate is incubated with the enzyme aldolase under standard -

conditions?

F-1,6-BP DHAP + G-3-P Keq = 6.43 x 10-5

I 1×10 'm ② 0

É×¥×××#
from quad 6--0.00022

__Y•¥gm
6-4.3×0-5
④ qAp]=[☒3PJ=0 -00022M

-1×10-4=78×0-4
[Ft6BP]
Ñ4o→=× cheek :k=§%¥¥
-6.2*0-5 -

2-53×10-4
goodennfofxofinquadratic
[Link]?-*ooi--
=
✗

25% use
This study source was downloaded by 100000870842035 from [Link] on 04-29-2025 09:08:33 GMT -05:00
-
nextpage
c
ego

E 1×10-3 -

✗ ✗ ✗

6-4.3×0-5__✗_.
1×10-3 ✗ -

43×10*-6.43×18 I
2
✗
6.
=

0=17+6.43×10-5 6.43×0-58 -

✗
-0-0022
-
a -0*29
Carboxylic acids are organic molecules that undergo a limited dissociation to make
carboxylate salts and hydronium ions.

Please predict the hydronium ion concentration for a 0.10 M benzoic acid solution. The acid
dissociation constant for benzoic acid (Ka) is 6.46 x 10-5 at 25oC.

-Éo¥§Ñ €IÉIg+HHg
'

I 0,1M 14--6.46×0-5
small
? g-
✗

c
-

✗
a +✗ 1- ✗

E
approx
0.1×6.46×10-5=14
Ka=o¥*= ¥ V6.46 ✗ 10-6=55
This study source was downloaded by 100000870842035 from [Link] on 04-29-2025 09:08:33 GMT -05:00
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