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Microscope Usage and Calculations Worksheet

The document provides information on using compound microscopes, detailing the magnifying power of eyepiece and objective lenses. It includes instructions for calculating total magnification and measuring actual sizes of specimens. Additionally, it outlines the parts of the microscope, proper handling techniques, and focusing methods.

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tony774135
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12 views3 pages

Microscope Usage and Calculations Worksheet

The document provides information on using compound microscopes, detailing the magnifying power of eyepiece and objective lenses. It includes instructions for calculating total magnification and measuring actual sizes of specimens. Additionally, it outlines the parts of the microscope, proper handling techniques, and focusing methods.

Uploaded by

tony774135
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

USING MICROSCOPES

Maqnifying Power:
A compound microscope has two sets of lenses. The lens you look through is called the
eyepiece lens. The lens near the specimen being examined is called the objective lens.
The objective lens is one of three or four lenses located on a rotating turret above the
stage, and that vary in magnifying power. The lowest power is called the low power
objective (LP), and the highest power is the high power objective (HP).You can determine
the magnifying power of the combination of the two lenses by multiplying the magnifying
power of the eyepiece lens by the magnifying power of the objective that you are using.

For example, if the magnifying power of the eyepiece is 10 (written and the
magnifying power of an objective is 4 (4)(), the magnifying power of that lens
combination is If an object is magnified 40 times, the image you see is 40 times
larger than the object.
Our school microscopes have the following lens magnifications:
Eyepiece = I OX Low power objective =
4X
Medium power objective = I OX High power objective =

1. Calculate the total magnifying power of the following lens combinations:


Eyepiece and low objective combined =

Eyepiece and medium power objective combined =

Eyepiece and high power objective combined =

2. The following images were photographed Using a compound microscope.


Measure the images and Use the magnification of the lenses to calculate the
actual size:
Actual width = measurement + magnification = mm

mm
urn

1 Millimetre is equal to 1000 Micrometre. To convert from millimetres to micrometres, move


1 mm = 1000 pm the decimal 3 places to the right (x 1000)

USING
MICROSCOPES
l . Label the parts of the microscope using the list below.

Stage Course focus knob Barrel


Arm Base Stage clips
Fine focus knob Light source Eyepiece lens
Iris Adjustment Revolving 4x Objective lens
nosepiece
IOx Objective lens 40x Objective lens
2. YOU should always carry a microscope with two hands. Which parts do you
hold while carrying the microscope?

3. What is the purpose of the stage clips?

4. Which objective lens should you use first and why?


5. How should you focus a specimen under a microscope?

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