Class: _________________
Student name: ___________________________________ Lecturer's signature:
Student ID: ____________________________________
HCM City date____month ____year______
Lab:……………………………..
Questions to prepare before class:
1. Describe 3 reasons why it is necessary to ensure sterile conditions when inoculating.
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2. Describe 2 ways to use an alcohol lamp to ensure sterile conditions when inoculating.
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3. How to avoid contamination from the air when inoculating?
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4. Why is it necessary to write information on the agar slant? What information needs to be
written on the agar slant after inoculation?
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5. Name the disinfectant solution used.
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6. The following disinfection procedures must be performed:
(a) before starting the operation
(b) after finishing the experiment
(c) after finishing each operation
(d) Both b and c
(e) All of the above
7. After sterilizing the inoculation loop, to open the stopper of the culture tube, you need to do the
following:
(a) do not put the inoculation loop on the workbench, use your teeth to open the stopper of the culture
tube
(b) open the stopper of the culture tube and hold it on the top of the inoculation loop handle
(c) open the stopper of the culture tube with the inoculation loop handle and place it on the workbench
and then perform the next operation
(d) open the stopper of the culture tube and hold it on the top of the inoculation loop handle
(e) All of the above methods are applicable
8. To sterilize the inoculating rod with an alcohol lamp, you need to do the following:
(a) heat it over a flame 1 to several times
(b) burn it over a flame for 5 minutes
(c) burn it over a flame until the tip of the inoculation loop is red hot
(d) burn it over a flame until the loop red hot inoculation
(e) burn over flame until tip and shaft of inoculation loop are red hot