BCH 211 Tutorial Questions
1. The following are criteria required for the selection of buffers for biological research except:
a. They must not be toxic
b. they should form complexes with all cations
c. they should be enzymatically and hydrolytically stable
d. they should be available in high degree of purity
2. Percentage by weight is indicated by
a. x%v/v
b. x%w/v
c. x%w/w
d. x%v/w
e. x%v/w
3. Water is incompletely dissociated because
a. It is polar
b. none of the above listed
c. it is an ion
d. it has a limited tendency to ionize
e. there is continuous dissociation and reassociation
4. The acceptable buffering capacity is within the range
a. pH=pKa more or less by 0.5
b. pH=pKa more or less by 0.2
c. pH=pKa more or less by 1
d. pH=pKa more or less by 2
5. A weak acid is one
a. That dissociates completely in water
b. that partially dissociates in water
c. that is totally undissociated in water
d. all of the above
e. none of the above
6. At very low concentrations
molality of a solution is higher than its molarity
a. correct
b. wrong
molarity of a solution is higher than its molality
a. correct
b. wrong
molarity and molality of a solution tend to be equal
a. correct
b. wrong
7. A worker accidentally spilled 100 cm3 of a solution of lead sugar (Pb (CH3COO)2.3H2O) containing
10 meq Pb per dm3 into a tank containing 5000 dm3 potable water. If the max limit of Lead in potable
water is 0.1 ppm calculate the amount of lead in the contaminated water (Pb=82).
a. 0.164ppm
b. 0.0164ppm
c. 1.64ppm
d. 1ppm
e. 82ppm
8. Convert 25% w/v solution of calcium II hydroxide to concentration in Molarity(Ca=40, H=1,O=16)
a. 3.38M
b. 33.8M
c. 0.338M
d. 0.34M
e. 3.4M
9. The hydrogen ion concentration of a given water is
10-6mol/dm3. What is the pH of the water?
a. 0.6
b. 6.0
c. 5.6
d. 6.6
e. 7.6
10. The triple point of water is 273.17K. at this temperature
a. water exist only in the liquid phase.
b. water exists only in the gaseous phase.
c. water exists only as ice.
d. water exist as liquid, ice, and water vapour.
e. none of the above
11. The shape of water molecule is
a. irregular tetrahedron
b. octahedron
c. pyramidal
d. tetrahedral
e. rhombic
12. The attraction between hydrogen and oxygen atoms of water holding the molecules together is
called
a. Van der Waal Force
b. Electrostatic force
c. Covalent bond
d. Hydrogen bonding
e. ionic bond
13. Which of the following is not a class of solution
a. hypertonic
b. supertonic
c. isotonic
d. hypotonic
e. saturated
14. --------------- is the most abundant component of an organism
a. Water
b. protein
c. carbohydrate
d. Blood
e. sodium ion
15. An acid is a substance ________
capable of accepting an electron
a. true
b. false
capable of donating an electron
a. true
b. false
capable of accepting a proton
a. true
b. false
capable of donating a proton
a. true
b. false
capable of donating an hydroxyl group
a. true
b. false
16. The dielectric constant of an aqueous solution reduces with the introduction of which of the
following substances?
a. ethanol
b. acetone
c. hydrochloric acid
d. sodium hydroxide solution
e. sodium chloride solution
17. Stability of native macromolecules is favoured by
a. hydrophobic interactions, salt bridges and hydrogen bonding.
b. hydrogen bonding only.
c. hydrogen bonding and hydrophobic interactions.
d. hydrophobic interactions.
e. salt bridges.
18. Biomembranes are stabilized by
a. hydrophobic interactions between the hydrocarbon chains of fatty acid molecules.
b. hydrophilic interaction of the non-polar heads of fatty acid molecules and the surrounding.
c. hydrophilic interactions between polar and nonpolar sections of fatty acids.
d. hydrophobic interactions between polar heads of fatty acids and the aqueous environment.
e. none of the above
19. Ka for acids A,B,C,D and E are 5.37 x 10-2 , 7.41 x 10-4, 8.91 x 10-9, 6.17 x 10-8, 8.13 x 10-
7 respectively. Arrange the acids in order of increasing strength.
20. The anomalous expansion of water when it cools from 4o Celsius to zero has biological
importance which include
a. Protection of aquatic life in the temperate regions as denser water at 4o C remains below ice
in frozen ponds and seas.
b. destruction of bacteria cells by ice.
c. elimination of dissolve impurities from sea water.
d. absorption of carbon dioxide from the atmosphere.
e. release of biological wastes in gaseous form from the bodies of frozen water.
21. Water is widely distributed to the following spaces except
a. intracellular
b. intramuscular
c. extracellular
d. intravascular
e. interstitial
22. What is the pH of a solution having hydroxyl ion concentration of 4 x10-4?
a. 6.1
b. 1.6
c. 10.6
d. 1.06
e. 1.1
23. Which of the following has the highest available energy
a. formaldehyde
b. methanol
c. carbon dioxide
d. formic acid
24. Given that the concentrations of H2PO4- and HPO42- in a buffer are 0.04M and 0.06M respectively,
what is the pH of the buffer (pKa =7.21)?
a. 7.8
b. 7.2
c. 7.4
d. 8.0
25. The following do not meet all the criteria for selecting buffers used in biological research except
a. Tris.
b. Hepes.
c. Phosphate.
d. none of the above.
26. All the following reduce ubiquinone to ubiquinol except
? NADH-coenzyme Q reductase
? succinate-coenzyme Q reductase
? co-enzyme Q-cytochrome C reductase
? sn-glycerophosphate dehydrogenase
27. The overall redox potential for the reoxidation of NADH in the electron transport chain is
a. 1.136 V
b. 0.816 V
c. -0.32 V
d. -2.30 V
28. Given that the overall redox potential of a reaction in which two electrons are transferred is
0.06V, calculate the change in Gibb's Free energy
a. -11.58 kJ/mol
b. -12.36 kJ/mol
c. -7.3 kJ/mol
d. -8.39 kJ/mol
29. In the Process of coating a strip with aluminium, a current of 5A was applied for 30 min. What is
the mass of Aluminium deposited?
a. 3.36g
b. 1.56g
c. 0.84g
d. 0.25g
30. Osmosis was first described in ________
a. 1748
b. 1848
c. 1784
d. 1788
e. 1789
31. Which of the following is correct of the Henderson-Hasselbach's equation?:
a. pH=pK+log [A-]
b. pK= pH+ log([A-]/[HA])
c. pH = pK+ log1
d. pH = pK
e. pH = logK
32. The angle between hydrogen atoms of water is approximately ______
a. 103o
b. 105o
c. 100o
d. 125o
e. 115o
33. How many equivalents of Ca2+ is present in 50 ml of 0.5 mole/dm3 solution of calcium chloride?
a. 2.5 equivalents
b. 0.025 equivalents
c. 125 equivalents
d. 0.125 equivalents
e. 2.5 equivalents
34. How many moles of sodium hydroxide will be required to neutralize 100cm3 of 1M H2SO4?
a. 0.4
b. 2
c. 0.2
d. 0.1
e. 1.0
35. The pH of 0.0000025M H2SO4 is
a. 5.3
b. 2.7
c. 5.6
d. 3.5
36. 0.1dm3 of 1M sodium hydroxide is mixed with 0.9 dm3 of 1M acetic acid. what is the pH of the
resultant solution if the pKa of acetic acid is 4.61?
a. 3.10
b. 4.10
c. 3.9
d. 9.1
e. 8.9
37. A strong acid in aqueous solution is……………..
a. fully dissociated.
b. partly dissociated.
c. undissociated.
d. neutral.
e. disintegrated.
38. A buffer is required to
a. increase the pH of a medium
b. decrease the pH of amedium
c. resist change in the pH of a medium
d. all the above
e. none of the above
39. A 2% w/v solution calcium chloride expressed as mol/dm3 is
a. 5 mol/dm3
b. 0.5 mol/dm3
c. 0.08 mol/dm3
d. 0.02 mol/dm3
e. 2.0 mol/dm3
40. The following best define buffers as systems capable of …….
a. absorbing protons and electrons thereby resisting pH changes.
b. absorbing conjugate acids and conjugate bases thereby resisting pH changes.
c. absorbing protons and hydroxyl ions thereby resisting pH changes.
d. recombining hydrogen ion and conjugate base to form conjugate acid.
e. releasing protons only thereby resisting pH changes.
41. Polyprotic acids are capable of dissociating to give
a. more than one mole of hydroxyl groups per mole
b. more than one mole of protons per mole
c. less than one mole of protons per mole
d. less than one mole of conjugate acid per mole
e. none of the above
42. In the presence of a strong base, NH4+ ion behaves as
a. a base
b. an acid
c. a weak base
d. a strong acid
e. none of the above
43. How many equivalents of H+ is present in 0.5M H3PO4?
a. 0.5
b. 1.5
c. 1.0
d. 0.05
e. 15.0
44. which of the following act as a physiological buffer in animal cells?
a. proteins
b. sodium chloride
c. potassium chloride
d. ammonia
e. urea
45. Biomembranes are stabilized by ………………
a. hydrophobic interactions between the hydrocarbon chains of fatty acid molecules.
b. hydrophilic interaction of the non-polar heads of fatty acid molecules and the surrounding.
c. hydrophilic interactions between polar and nonpolar sections of fatty acids.
d. hydrophobic interactions between polar heads of fatty acids and the aqueous environment.
e. none of the above.
46. A weak acid is one
a. that dissociates completely in water.
b. that partially dissociates in water.
c. that is totally undissociated in water.
d. all the above.
e. none of the above.
47. Calculate the pH of 2.5 x 10-5 M H2SO4 (aq)
a. 3.5
b. 4.7
c. 3.6
d. 5.6
e. 4.3
48. The hydrogen ion concentration of a given water is 10-6mol/dm3. What is the pH of the water?
a. 0.6
b. 6.0
c. 5.6
d. 6.6
e. 7.6
49. Which of the following is not a product of hydrophobic interractions of amphiphiles in an
aqueous environment?
a. liposomes
b. lipid bilayers
c. micelles
d. emulsions
e. homogenous solution
50. The anomalous expansion of water when it cools from 4o Celsius to zero has biological
importance which include …………
a. Protection of aquatic life in the temperate regions as denser water at 4o C remains below ice
in frozen ponds and seas.
b. destruction of bacteria cells by ice.
c. elimination of dissolve impurities from sea water.
d. absorption of carbondioxide from the atmosphere.
e. release of biological wastes in gaseous form from the bodies of frozen water.
51. A worker accidentally spilled 100cm3 of a solution of lead sugar (Pb (CH3COO)2.3H2O) containing
10meq Pb per dm3 into a tank containing 5000dm3 potable water. If the max limit of Lead in potable
water is 0.1ppm calculate the amount of lead in the contaminated water(Pb=82).
a. 0.164 ppm
b. 0.0164 ppm
c. 1.64 ppm
d. 1 ppm
e. 82 ppm
52. Acetone is not likely to be a good solvent for biological solutions because……………
a. it has a very high dielectric constant.
b. it is apolar solvent.
c. it is nonpolar and has a low dielectric constant.
d. it cannot participate in hydrogen bonding.
e. it is an excellent aqueous medium.
53. Urine is----------
a. acidic.
b. alkaline.
c. neutral.
d. amphoteric.
e. acidobasic.
54. The following are functions of water in a living system except …...........
a. it provides a fluid system for the physicochemical processes of life.
b. it provides an opportunity for the beginning of life.
c. it participates as areactant in metabolic conversions.
d. it is an essential nutrient for photosynthesis.
e. it is a good solvent for macromolecules.
55. The property of water as a solvent
a. it is a polar molecule.
b. it is electrically charged.
c. it is asymmetric.
d. it has thermal properties.
e. it is apolar.
56. Which of the following is not a class of solution?
a. hypertonic
b. supertonic
c. isotonic
d. hypotonic
e. saturated
57. The water molecule exhibit polarity because …………….
a. the oxygen part has a net negative charhe and the hydrogen part has a net positive charge.
b. the oxygen and hydrogen components are neutral.
c. the oxygen part has a net positive charge and the hydrogen part has a net negative charge.
d. the oxygen and the hydrogen parts have a net positive charge.
e. none of the above.
58. Which of the following is not a property of water?
a. vaporizing property
b. freezing property
c. surface tension
d. clustering property
e. Thermal property
59. Water is incompletely dissociated because
a. It is polar.
b. none of the above listed.
c. it is an ion.
d. it has a limited tendency to ionize.
e. there is continuous dissociation and reassociation.
60. concentration is a measure of...................
a. quantity of solvent dissolved in a solute.
b. quantity of solute dissolved in a solvent
c. quantity of solute dissolved in a solution.
d. quantity of solution dissolved in a solute.
e. quantity of solvent dissolved in solution.
61. What is the pH of a solution which has [H+] of 3.2 x 10-4M
a. 3.8
b. 3.5
c. 3.6
d. 2.5
e. 4.2
62. What is the pH of a solution having hydroxyl ion concentration of 4 x10-4?
a. 6.1
b. 1.6
c. 10.6
d. 1.06
e. 1.1
63. Given that the concentrationsof H2PO4- and HPO42- in a buffer are 0.04M and 0.06M
respectively, what is the pH of the buffer(pKa=7.21)?
a. 7.8
b. 7.2
c. 7.4
d. 8.0
64. The acceptable buffering capacity is within the range
a. pH=pKa more or less by 0.5
b. pH=pKa more or less by 0.2
c. pH=pKa more or less by 1
d. pH=pKa more or less by 2
65. The following are the molecular species involved in the electron transport chain except
a. iron-sulphur proteins
b. protein bound Cu
c. cytochromes
d. protein-bound Ni
66. At pH=pK of an acid, the buffering capacity of its buffer system is
a. zero
b. moderate
c. highest
d. lowest
67. The following are physiological buffer systems except
a. bicarbonate
b. phosphate
c. Pipes
d. Haemoglobin