Scribd
Upload
5 views2 pages

CRISPR Lab Post-Experiment Questions

Uploaded by

Thomas
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
5 views2 pages

CRISPR Lab Post-Experiment Questions

Uploaded by

Thomas
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

CRISPR Post Lab Questions Name: _________________________

3 points/question

CRISPR Lab
1. In your own words, describe the process of CRISPR at the molecular level. How is each
molecular component integral to the process?

2. What does the acronym CRISPR stand for? Why have bacteria evolved to maintain these
repeats in their DNA sequences?

3. What gene editing did we do in this lab? Please be specific (what was actually altered at the
DNA level and how did this affect the resulting protein?). How would we know from our
results if we were successful in the gene editing process?
4. Describe your results from the CRISPR lab. Please give quantitative (expressed in numbers)
and qualitative (expressed as characteristics) information here.

5. Did your results show that gene editing did or did not occur?

6. There was no control in this experiment, which is worrisome. If you could design a control
for the experiment, what would it be? What confounding factors would it eliminate?

7. What are three different steps in the procedure that could have gone wrong, causing no
bacterial growth? What is the purpose of each of these steps?

You might also like