API® System
Fadia Nur A. Tita - Application Specialist (081216075598)
PT Indolakto Plant Ciracas, 14-15 September 2023
Outline
1. API Systems Principle
2. API Strips Guide
3. API 20 E Components and Workflow
4. API 20 NE Components and Workflow
5. Result Interpretation
6. API Strips Workflow
Section 1
API System Principle
Conventional biochemical test
Colorimetry principle: color change after biochemical reaction took place
API 20E test
API 20 E
API® (Analytical Profile Index) 20 E is a qualitative, standardized system for the identification of
Enterobacteriaceae and other non-fastidious Gram-negative rods. It contains 20 tubes with
different dehydrated substrates.
Type of tests performed
Section 2
API Strips Guide
API Strips List
General Bacteria Classification
Strip Selection Guidance
Strip Selection Guidance
Strip Selection Guidance
Section 3
API 20E Components
Section 4
API 20E Workflow
API 20E Workflow
1. Orientation test and
Gram staining/oxidase/catalase
strip selection
2. Culture incubation Culture is incubated accordingly
3. Suspension and strip Colony being suspensed in suspension medium,
inoculation homogenized, and inoculated into the strip’s tubes
Result examination manually, inputting the
4. Reading and
biochemical test to APIWeb, and interpreting the
interpretation
results
Microscopic Examination – Gram Staining
Observation at magnificence x1000, immersion oil
Orientation Test: Catalase
catalase
2 H2O2 2 H2O + O2
Orientation Test: Oxidase
API 20E
Culture age: 18-24 hours
Apply the mineral oil only for the underlined
tube like ADH
Section 5
Result Interpretation
Go to APIWeb [Link]
Confidence Levels
Other examples
Other examples
Other examples
Section 6
API Strips Workflow
API 50 CHB
API 50 CHL
API C AUX
THANK YOU