Scenario 1A
Automated Testing
Anti-A Anti-B Anti-D A1 Cells B Cells
3+ 0 0 1+ 3+
Forward Interp: Reverse Interp:
Tube Testing
Anti-A Anti-B Anti-A1 A1 Cells A2 Cells B Cells
Lectin
4+ 0 0 1+ 0 3+
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Scenario 1B
Automated Testing
Anti-A Anti-B Anti-D A1 Cells B Cells
0 3+ 3+ 2+ 1+
Forward Interp: Reverse Interp:
Tube Testing
Anti-A Anti-B Anti-A1 A1 Cells A2 Cells B Cells
Lectin
0 4+ NT 2+ 1+ 1+
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Gain of Antibody-Some Causes
• ABO subgroups
• Cold reacting antibodies (anti-M, anti-I)
• Reagent antibodies
• Rouleaux/hypergammaglobulinemia
• Transfusion (plasma, platelets)
• IVIG
• Transplantation
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Scenario 2
Tube Testing
Anti-A Anti-B Anti-D A1 Cells B Cells
3+ 1+ 3+ 0 4+
Forward Interp: Reverse Interp:
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Gain of Antigen-Some Causes
• Transplantation
• Rouleaux
• Acquired B
• B(A) or A(B) phenotype
• Transfusion
• Chimera
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Scenario 3A
Automated Testing
Anti-A Anti-B Anti-D A1 Cells B Cells
4+ 0 0 0 0
Forward Interp: Reverse Interp:
Tube Testing
Anti-A Anti-B Anti-D A1 Cells B Cells
4+ 0 0 0 1+
Forward Interp: Reverse Interp:
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Scenario 3B
Automated Testing
Anti-A Anti-B Anti-D A1 Cells B Cells
0 0 4+ ? 0
Forward Interp: Reverse Interp:
Tube Testing
Anti-A Anti-B Anti-A,B A1 Cells A2 Cells B Cells
0 0 0 0 0 0
Forward Interp: Reverse Interp:
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Loss of Antibody-Some Causes
• Very young or very old
• Immunosuppressed (e.g., congenital)
• Leukemia/lymphoma/myeloma
• ABO subgroup
• Transplantation
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Scenario 4
Automated Testing
Anti-A Anti-B Anti-D A1 Cells B Cells
0 0 4+ 4+ 0
Forward Interp: Reverse Interp:
Tube Testing
Anti-A Anti-B Anti-D A1 Cells B Cells
0 0 4+ 4+ 0
Forward Interp: Reverse Interp:
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Scenario 4
Tube Testing—Washed RBCs
Anti-A Anti-B Anti-D A1 Cells B Cells
0 3+ 0 4+ 0
Forward Interp: Reverse Interp:
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Loss of Antigen-Some Causes
• Transplantation
• Transfusion
• A or B subgroups
• Leukemia/lymphoma
• Excessive soluble blood group substance
(e.g., pancreatic, stomach, ovarian cancer)
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Scenario 5A
Tube Testing
Anti-A Anti-B Anti-A,B Anti-D
2+ mf 0 3+ 4+
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Scenario 5A
Transfusion. 2010 Jul;50(7):1571-80. doi: 10.1111/j.1537-2995
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)
Scenario 5B
0 4+mf 0 4+ 2+ 0
Type O- (95%) and Type B+ (5%)
Mother B, Father O
Pruss et al. Transfusion Vol 43, October 2003
This slide provided to [Link] by Dr. Nicole Draper for educational purposes only (see [Link]/054)