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Understanding Scientific Methods and Biology

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Understanding Scientific Methods and Biology

bio notes

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MusicFan101
Copyright
© All Rights Reserved
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Science: using evidence to solve problems that are hard/impossible to solve

#1: science organizes things in a way that makes the world easier to understand
#2: science changes over time, accumulated over 1000’s of years

Biology: study of life and living organisms

Scientific process starts with observations, we uses our senses to gather information
When 1 or more senses are eliminated, the brain focuses on a remaining sense.
The information that is gathered is called data and there are 2 types of data.

#1: quantitative: involves numbers: ex: 7 birds


#2: qualitative: characteristics that aren’t easily measured: ex: color, shape, texture, etc

Observations are followed with inferences. An inference is a theory based on the evidence that’s
given. Inferences aren’t always correct.

Inferences are based on your prior knowledge and experience and they can change with age.
When you are younger, you go along with the values that were instilled in you and as you age
you are exposed to other beliefs and your values change and the same thing can happen with
science.

Scientific method: process that everyone used to solve a problem


#1: problem based on your observation
#2: hypothesis (educated guess)
#3: create an experiment
#4: collect data (tables, graphs, etc)
#5: Conclusion: Did the data support your hypothesis?
Your conclusion may not support your hypothesis and you don’t change your hypothesis to go
with the new data, the work isn’t changed. Science is an ongoing process and based on
judgements. Findings are subject to revision and change.

Rules of the Experiment:


Control group: things that are kept the same: same amount of subjects/materials
Placebo effect: positive effect created by the placebo and the subject thinks that the placebo is
helping them.

The experimental group(s) are tested with 1 variable. Variables are never mixed.

The experimental group is compared to the control group


No experiment is a waste of time, there is always something to be learned from the experiment.
Smallest to largest units
#1: molecules (nonliving)
#2: cells (smallest living thing)
#3: tissues
#4: organ
#5: organ systems (reproductive systems, skeletal systems, etc)
#6: organism (organs come together to make one, think school)
#7: population (only applies to 1 species)
#8: community (made of different populations are species)
#9: ecosystems (includes living and nonliving)
#10: biome (desert, tundra, temperate deciduous forest, etc)
#11: biosphere (earth)

Metric system is based on multiples of ten (10, 100, 1000, etc)

Celsius: standard unit of temperature (0 is freezing, boiling is 100)


Meter: standard unit of length
Liter: standard unit of volume
Gram: standard unit of mass

Tools used by biologists:

Microscopes: enlarge objects


Light Microscope: uses light to bend rays using lenses (can see living subjects)
Electron Microscope: uses beam of electrons and vacuums to see subject (no living subjects, this
will kill them)

TEM: Transmission electron microscope (transmits electrons to obtain image)


SEM: scanning electron microscope (bombards subjects with electrons to create 3D image)
Ultrasound uses sound waves to obtain the image
Chapter 2 Bio Notes:
Scientific Approaches

Holistic: approach that looks at the big picture first before treating a specific are
Reductionist: approach that looks at the individual components of a system first. Reductionists
start at the bottom of the hierarchy of biological structure and holists start at the top.
23 chromosomes from 1 parent and 23 from the other parent.

Matter: has mass and takes up (doesn’t have to be seen)\


Chemical elements make up all mass, 92 naturally occurring, 25 are needed for life, calcium (C),
oxygen (O), hydrogen (H), nitrogen (N), iron, sodium, carbon, etc

H,O,N,C make up 96% of the body Trace elements: needed in small amounts (iron, iodine, etc)
Things like goiter can happen when you don’t get the trace amounts.

Compounds: substances created by combining 2 or more elements in a fixed ratio (H20 (water)
NaCal (sodium chloride) C6 H12 O6 (sugar)
Mass number = atomic weight (protons + neutrons)
Isotopes = atom with the same # of protons but different # of neutrons
Carbon-12 is normal Carbon-13 and 14 have different neutron #s

Radioactive isotopes: can be used to trace biological processes in plants and animals

Electron shells: # in the outermost shell determines the chemical activity of an element (1st shell
holds 2, 2nd shell holds 8)
Unstable elements become stable when they combine with other unstable elements.
Ion: atom/molecule with an electric charge from gain (-) / loss (+) of electrons.
Ionic Bond: (NaCl) attraction that holds elements together. crystals/salt: synonym for ionic bond
+ Tells you it lost electron(s), - tells you it gained electron(s)
Covalent bonds: share electrons, form molecules
Molecular formula: H20, C6 H12 O6 Structural formulas: shows # of atoms and how they are
bonded.
Single Covalent: 2 atoms share 1 electron (c-c)
Double covalent: 2 atoms share 2 electrons (c=c)
Triple covalent bond: 2 atoms share 3 electrons (c = c)

Each element is made of atoms. Atoms are the smallest unit of matter
Made of smaller particles called protons, neutrons, and electrons. Protons and neutrons are in the
nucleus and the electrons are located outside of the nucleus.

Water: polar molecule, H has a slight positive charge, O has a slight negative charge. Atoms'
shared attraction for a shared electron is electronegativity. O has a stronger pull that H.

Water is a great solvent (dissolving agent in a solution). Water breaks down anything over time.
Solution: is a liquid made of a homogeneous mixture of 2+ substances.
Solvent: does the dissolving (water)
Solute: gets dissolved (sugar, salt, etc) Water is the solvent of life (cells, blood, plant sap, etc)
Suspensions: mixtures of water and nondissolved materials (blood)

Water exists as a solid, liquid, and a gas. Water is abundant in all 3 states and life can exist
because of it.

Cohesion: force of attraction between like things (water attracted to water)


Adhesion: attraction between 2 different things (water attracted to glass)
Capillary action: ability of a liquid to flow in narrow space using cohesion
Surface tension: measure of cohesive force on the surface of a liquid
VanderWaals forces: slight attraction that develops between oppositely charged regions of
nearby molecules

Water has the abilty to resist temperature change due to H bonding. Allows life to exist
Water moderates earth’s temperature by

#1: absorbing large amounts of heat


#2: evaporative cooling

Temperature: average amount of kinetic energy in an object (larger masses are warmer than
smaller ones)

Heat: total amount of energy in an object

H-bond: weak bond, attraction of an H atom with its slight positive charge to a negatively charge
atom such as O. Bonds are stable in ice.

pH: goes from 0-14, goes backwards, (0 is high pH, and 14 is low, basic) 7 is neutral
Acid: releases H+ in solution bases: OH- in solution

Common questions

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Water's structure makes it an excellent solvent due to its polarity and ability to form hydrogen bonds. The polar covalent bonds between hydrogen and oxygen create a distribution of charge that allows water to interact with various solutes. Oxygen's slight negative charge attracts positive ions, while hydrogen's slight positive charge attracts negative ions. This interaction facilitates the dissolution process, making water the 'solvent of life.' These properties enable it to dissolve ionic compounds and polar molecules effectively, critical for biological processes such as nutrient transport and waste removal .

The concept of pH is crucial in biological systems because it affects the structure and function of molecules, the rates of chemical reactions, and biological processes like metabolism. The pH scale runs from 0 to 14, with 7 being neutral. Values lower than 7 indicate acidity (release of H+ ions), while values higher than 7 signify basicity (release of OH- ions). Enzyme activity, cellular function, and overall organism health can be significantly affected by changes in pH, highlighting its importance in maintaining life .

Quantitative data is numerical and involves measurements or counts, such as the number of individuals in a population or the height of a plant. Qualitative data, on the other hand, describes characteristics or properties without the use of numbers, like color, texture, and shape. Both types of data are important in scientific research as they provide comprehensive insights; quantitative data offers precision and the ability to apply statistical analysis, while qualitative data provides context and depth, helping to explain patterns or behaviors that numbers alone may not .

The scientific method is foundational to ongoing scientific inquiry and discovery. It provides a structured approach for identifying and exploring problems through observation, hypothesis formulation, experimentation, and conclusion. This cyclical process allows scientists to continuously test hypotheses, refine theories, and adapt to new data and insights. The iterative nature of the scientific method ensures that scientific knowledge remains robust and dynamic, allowing for adaptation and growth as new technologies and understanding of the natural world develop .

Cohesion and adhesion are crucial to water’s function in biological systems. Cohesion, the attraction between water molecules, contributes to phenomena like surface tension, allowing for things like insects to walk on water surfaces. Adhesion, the attraction between water molecules and different substances, facilitates processes such as capillary action, which is essential for water transport in plants as it enables water to climb against gravity through plant vessels. Together, these properties ensure efficient water transport and stability in various biological and ecological processes .

Trace elements are essential in small amounts for various biological functions, including enzyme catalytic activities and structural roles in biomolecules. For instance, iron is crucial for oxygen transport in hemoglobin, while iodine is necessary for thyroid hormone production. Deficiencies in these trace elements can lead to diseases; for example, insufficient iodine can cause goiter, a thyroid gland enlargement. Therefore, trace elements, though required only in minute quantities, play vital roles in maintaining physiological processes and overall health .

Scientific knowledge undergoes revision over time due to its intrinsic nature of being tentative and self-corrective. As new observations and data emerge, existing theories and models are continually tested and refined or even replaced. This iterative process allows science to adapt to new evidence, leading to a deeper and more accurate understanding of the natural world. The willingness to embrace change in scientific explanations fosters progress and innovation; it underscores the fact that science is not just a collection of facts, but a dynamic process driven by inquiry and evidence .

Electron shell configurations influence an element's chemical activity primarily through the number of electrons in the outermost shell, known as valence electrons. These electrons determine how an element will interact and bond with others. Elements with full outer shells are generally inert, while those with incomplete outer shells are reactive as they tend to gain, lose, or share electrons to achieve stability. For example, elements with one electron in their outer shell, like sodium, are highly reactive, whereas noble gases, with full outer shells, are not .

TEM (Transmission Electron Microscope) and SEM (Scanning Electron Microscope) differ in their operational principles and applications. TEM creates images by transmitting electrons through a specimen, providing detailed internal structure images, making it ideal for examining thin, cellular, or molecular slices. SEM, however, bombards the specimen with electrons and records the resultant emitted electrons to produce 3D surface images, suited for detailed topographical studies. Both require non-living specimens due to the high-vacuum environment and electron bombardment .

Holism and reductionism take fundamentally different approaches to understanding biological systems. Holism looks at biological systems by understanding the complete picture first, treating the system as a whole before dissecting specific components. In contrast, reductionism starts with analyzing individual parts of the system and then builds up to understand the whole. Reductionists often start at the bottom of the hierarchy of biological structure, for example, at the molecular or cellular level, while holists begin with the organism or ecosystem level .

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