Assignment 02
Course Code: BTE 101
Course Name: Introduction to Biotechnology and Genetic Engineering
Section: 05
Submitted To – Sadia Noor Mou
Submitted By- Eusha Mashiat
Student ID: 24126130
Submission Date:27.04.24
Assignment 2
BTE101 (Sec 5)
Faculty: Sadia Noor Mou (SNU)
Part-A 5.0
The following diagram represents a nucleotide that serves as a monomer
for nucleic acids.
1) Would you classify the nitrogenous base in this nucleotide as Purine or
Pyrimidine? 0.5
2) Would you classify this nucleotide as a monomer for DNA or RNA?
Explain. 1.0
3) Assume that this monomer is added to the growing end of a nucleic acid
polymer.
Name the covalent bond that will be formed between the nucleic acid
polymer and
the incoming nucleotide.
0.5
4) If the monomer drawn above comprises 28% of a double-stranded
nucleic acid,
predict the percentages of each of the three remaining nucleotides present
in the
double stranded nucleic acid.
1.0
5) You compare the double-stranded nucleic acid containing 28% of this
monomer
to another double-stranded nucleic acid that is of the same length but
contains 10%
of this monomer. Which double-stranded nucleic acid would denature at a
lower
temperature, the one containing 28% or the one containing 10% of this
monomer?
Explain.
2.0
Part-A
Q.1. Answer: I would classify the nitrogenous base in this nucleotide as Pyrimidine.
Q.2. Answer: I would classify the nucleotide as a monomer for DNA since it has deoxyribose
sugar that lacks the -OH group at the 2’ C position.
Q.3. Answer: The name of the covalent bond that will be formed between the nucleic acid
polymer and the incoming nucleotide is the phosphodiester bond.
Q.4. Answer: The monomer drown above consists of nitrogen base Thymine. As per
Chargaff’s rule we know that the content of adenin equals to that of thymine (A=T) and
guanine equals to the content of cytosine (G=C). Since the monomer is a dTTP =28%, the
remaining percentage of the nucleotides are dATP = 28%, dCTP = 22%, and dGTP = 22%.
Q.5. Answer: T pairs with A forming 2 hydrogen bonds and C pairs with G forming 3
hydrogen bonds. The new double-stranded nucleic acid with 10% dTTP will have 10% dATP,
40% dCTP, and 40% dGTP. And the old double stranded nucleic acid that has 28% dTTP will
have 28% dATP, 22% dCTP and 22% dGTP. Therefore the number of 3 hydrogen bonds in
the new double-stranded nucleic acid is higher than in the old double-stranded nucleic acid.
So, the double-stranded nucleic acid that has 28% dTTP would denature at a lower
temperature compared to the double-stranded nucleic acid that has 10% dTTP.