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High-Level Disinfection with Hydrogen Peroxide

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0% found this document useful (0 votes)
137 views3 pages

High-Level Disinfection with Hydrogen Peroxide

Uploaded by

Dawit g/kidan
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Page 1 of 3

Name of the institution Effective


Institution from
Logo STANDARD OPERATING PROCEDURE (SOP) Review
HIGH LEVEL DISINFECTION (HLD) USING date
HYDROGEN PEROXIDE Version

SOP for HLD using Hydrogen Peroxide


1. Purpose
To guide HCWs how to perform high level disinfection using hydrogen peroxide

2. Scope
This SOP relates to all staff working in healthcare facilities who may be involved with
high level disinfection using hydrogen peroxide.

3. Responsibility
Performing high level disinfection using hydrogen peroxide is a responsibility of
trained medical personnel who use or handle the medical devices to ensure that
they are properly disinfected according to established protocols.

4. Equipment requirements:
 Personal protective equipment (gloves/utility gloves, gown/apron, protective
eyewear/goggle, mask, head cover and plastic shoe covers)
 Disinfectant solution – concentrated hydrogen peroxide (30-35%)
 Distilled water for dilution (to make a 3% solution)
 Bucket (4 or as per requirements)
 Cleaning supplies
 Disinfectant immersion tray
 Hazard/warning signs
 Sterile forceps
5. Risks
 Irritation and allergic reaction to skin and mucous membrane
 Chemical burn and other damage to the surface and other material
6. Key Safety Rules:
Page 2 of 3
Name of the institution Effective
Institution from
Logo STANDARD OPERATING PROCEDURE (SOP) Review
HIGH LEVEL DISINFECTION (HLD) USING date
HYDROGEN PEROXIDE Version

 Always wear personal protective equipment (PPE) including gloves, apron or


gown, and goggles or a face shield, to avoid contact with the solution.
 Ensure proper ventilation in the area and do not inhale the vapors produced by
the process.
 Follow the manufacturer's instructions and guidelines for the concentration,
application, and contact time of hydrogen peroxide solution.
 Do not mix hydrogen peroxide with other chemicals or substances, as it can lead
to an explosive reaction.
 Dispose of the used solution by pouring down the drain of a sink or into a toilet.
However, it is important to dilute the hydrogen peroxide solution first. For every
part of hydrogen peroxide solution, you should add 10 parts of water. This will
help to reduce the concentration of hydrogen peroxide and make it less harmful
to the Environment.
 Regularly check the integrity of the equipment and properly maintain and
calibrate the devices used in the process.
7. Procedures
1. Wear personal protective equipment (PPE), including gloves/utility gloves,
gown/apron, protective eyewear/goggle, mask, head cover and plastic shoe
covers)
2. Ensure that the equipment being disinfected is clean and free of any visible
debris or residue.
3. Dilute the hydrogen peroxide product according to the instructions provided by
the manufacturer and use the recommendations in the following table as a
general rule.
Page 3 of 3
Name of the institution Effective
Institution from
Logo STANDARD OPERATING PROCEDURE (SOP) Review
HIGH LEVEL DISINFECTION (HLD) USING date
HYDROGEN PEROXIDE Version

Concentration (w/v %) Water: Hydrogen Peroxide

1 part water: 10 parts hydrogen peroxide


3%
1 part water: 5 parts hydrogen peroxide
6%
1 part water: 3 parts hydrogen peroxide
10%

4. Completely immerse the equipment in the diluted hydrogen peroxide solution.


5. Allow contact of equipment with the solution for the recommended contact time
(typically 20 minutes).
6. Remove items using high-level disinfected sterile forceps or gloves.
7. Rinse the equipment thoroughly with sterile water to remove any residual
hydrogen peroxide.
8. Dry the equipment completely before use.
9. Discard the solution safely after use. (see above)
10. Use promptly or store in a dry, high-level disinfected dark and covered container.

8. Reference
 MoH. (2023). national infection prevention and control reference manual for
healthcare service providers and managers volume 1: general infection
prevention and control.
 Chromeextension://efaidnbmnnnibpcajpcglclefindmkaj/https://
[Link]/wp-content/uploads/sites/46/2023/04/SOP-Template-
for-Hydrogen [Link]
 chromeextension://efaidnbmnnnibpcajpcglclefindmkaj/[Link]
media/documents/decontaminating-with-hydrogen-peroxide-safety [Link]

Common questions

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The procedure document ensures compliance by outlining detailed PPE requirements, establishing strict guidelines for the concentration and application of hydrogen peroxide, and stipulating necessary safety measures like ventilation and monitoring equipment integrity. Adherence to these protocols helps align with safety and effectiveness standards, preventing health hazards and ensuring thorough disinfection .

After disinfection with hydrogen peroxide, equipment should be thoroughly rinsed with sterile water to remove any residual disinfectant. Following this, the equipment needs to be dried completely to prevent any residual moisture that could lead to contamination or rust. Finally, it can be used promptly or stored in a dry, disinfected, dark, and covered container .

The procedure ensures effective disinfection by requiring that equipment is clean and free of visible debris before immersion in hydrogen peroxide solution. The hydrogen peroxide is then diluted according to manufacturer instructions and concentration guidelines, and equipment is completely immersed in the solution for the recommended contact time, typically 20 minutes. Using disinfected sterile forceps or gloves, the equipment is removed and thoroughly rinsed with sterile water to remove residual hydrogen peroxide, then dried completely before use .

Primary safety precautions for HLD with hydrogen peroxide include wearing personal protective equipment (PPE) such as gloves, gown or apron, and goggles or a face shield to avoid contact with the solution . Proper ventilation is important to prevent inhalation of vapors, and the manufacturer's instructions for concentration, application, and contact time must be followed. Additionally, hydrogen peroxide should not be mixed with other chemicals to avoid explosive reactions, and used solutions must be diluted before disposal to minimize environmental harm .

Proper ventilation is crucial during high-level disinfection with hydrogen peroxide to prevent the inhalation of vapors, which can be harmful to health. Hydrogen peroxide vapors can irritate respiratory organs and lead to potential health hazards; thus, ensuring a well-ventilated area mitigates these risks and ensures the safety of personnel involved .

The specific equipment required includes personal protective equipment such as gloves, gown or apron, protective eyewear like goggles, a mask, head cover, and plastic shoe covers. Other necessary items are the concentrated hydrogen peroxide disinfectant solution, distilled water for dilution, buckets, cleaning supplies, a disinfectant immersion tray, hazard/warning signs, and sterile forceps .

To prevent explosive reactions, it's critical not to mix hydrogen peroxide with other chemicals or substances during the disinfection process. Additionally, following the manufacturer's instructions for concentration and application, and ensuring proper handling and storage conditions are key preventive measures .

Used hydrogen peroxide solution should be diluted with water before disposal to reduce its concentration and environmental impact. The recommended dilution is one part hydrogen peroxide solution to ten parts water. Once diluted, it can be safely poured down a sink or toilet .

Risks associated with using concentrated hydrogen peroxide include irritation and allergic reactions to the skin and mucous membranes, chemical burns, and potential damage to surfaces and materials . Proper handling with appropriate personal protective equipment and adherence to safety guidelines are necessary to mitigate these risks .

Using sterile forceps or gloves is crucial to prevent recontamination of sterilized equipment. Since the goal of high-level disinfection is to eliminate microbial threats, handling equipment with non-sterile tools or hands can introduce contaminants, compromising the disinfection process .

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