Date 29/09/2023 Srl No.
4 Breed LABRADOR RETRIEVER
Pet Name . ESKA Age 7 Yrs. 5 Mn. 1 Day Sex F
Owner VIDYAUM RATHOR CLINIC Printed on 29/09/2023
Ref. By Dr. SHALLY Contact No.
Species CANINE
VET PCR
R T PCR TICK PANEL
MOLECULAR BIOLOGY
Test Performed: Real-Time PCR
Sample: Blood in EDTA
Method / Technique:
Genomic DNA was isolated using Blood DNA extraction [Link] test was performed using gene specific advanced
based Realtime polymerase chain reaction (PCR) on BIOER This test panel detects specific Babesia canis,
Babesia gibsoni, Hepatozoon canis, Anaplasma platys, Ehrlichia canis Organisms.
RT PCR ADVANCE TICK PANEL
[Link] Test Organism Result Ct Value IC value
1. [Link] NEGATIVE 00 22.3
2. Anaplasma Platys NEGATIVE 00 27.1
3. Babesia canis NEGATIVE 00 21.4
4. Babesia gibsoni NEGATIVE 00 28.3
5. Hepatozoon canis NEGATIVE 00 23.2
6. Babesia Vogeli NEGATIVE 00 26.1
7. Babesia Rossi: NEGATIVE 00 22.1
Not for Vetro - Legal purpose Page 1
Date 29/09/2023 Srl No. 4 Breed LABRADOR RETRIEVER
Pet Name . ESKA Age 7 Yrs. 5 Mn. 1 Day Sex F
Owner VIDYAUM RATHOR CLINIC Printed on 29/09/2023
Ref. By Dr. SHALLY Contact No.
Species CANINE
8. Trypanosoma Evansi NEGATIVE 00 26.3
9. Anaplasma Phagocytophilum NEGATIVE 00 25.2
10. Ehrlichia ewangii NEGATIVE 00 24.9
Impression: NEGATIVE
NOTE:- Result will be negative if CT value comes above 35.
Babesia Canis:
Babesia canis is blood protozoa that parasitize erythrocytes causing anemia in the infected dog. It is one of the most common infection that
is transmitted by the ticks present on the dog skin. Babesia canis is thought to cycle primarily between domestic dogs and Rhipicephalus
sanguineus the brown dog [Link] of canine babesiosis may present with wide variation of severity of clinical signs, ranging from a
hyperacute, shock-associated, hemolytic crisis to an inapparent, subclinical infection.
Babesia Gibsoni:
Babesia Gibsoni is blood protozoa that parasitize erythrocytes causing anemia in the infected dog. It is one of the most common tick born
infection that is transmitted by the ticks present on the dog skin.
Babesia gibsoni hemolytic anemia, thrombocytopenia, lethargy and splenomegaly. In many cases, treatment is frustrating, low levels of
parasites remain and a chronic subclinical carrier state develops.
Babesia Vogeli:
Babesia Vogeli is one of the main cause of Babesiosis in Dog. The protozoa is transmitted by Rhipicephalus Sanguineus ticks which has
a global distribution but is most prevalent in tropical and subtropical regions. Babesia Vogeli is regarded as being only mildly pathogenic
causing subclinical or only mild disease. B. Vogeli infections, severe complications can develop in dogs infected with other species
including systemic inflammatory response syndrome (SIRS), multiple organ dysfunction syndrome (MODS), refractory hypotension and
septic shock.
Babesia Rossi:
Babesia Rossi is blood protozoa that parasitize erythrocytes causing anemia in the infected dog. It is one of the most common ticks born
infection that is transmitted by the ticks present on the dog skin. The protozoa get transmitted by Haemaphysalis elliptica and possibly H.
leachi. Babesia rossi causes peracute and acute disease, the clinical signs including pale mucous membranes, depression, tachycardia,
tachypnoea, anorexia, weakness, splenomegaly and fever. The clinical signs are attributed to tissue hypoxia resulting from anaemia and a
Not for Vetro - Legal purpose Page 2
Date 29/09/2023 Srl No. 4 Breed LABRADOR RETRIEVER
Pet Name . ESKA Age 7 Yrs. 5 Mn. 1 Day Sex F
Owner VIDYAUM RATHOR CLINIC Printed on 29/09/2023
Ref. By Dr. SHALLY Contact No.
Species CANINE
concomitant systemic inflammatory response syndrome caused by marked cytokine release. The severe form of the disease is characterised
by haemolytic anaemia and severe acid-base derangements with secondary multiple organ failure and complications such as acute renal
failure, hepatopathy with marked icterus, hypoglycaemia acute respiratory distress syndrome, cerebral pathology and additional immune-
mediated erythrocyte destruction. Mortality is around 12%. A feature of the disease is that, in contrast to babesiosis in other domestic
species, pups and immature dogs are also badly affected.
Anaplasma Platys:
Anaplasma species are obligate intracellular tickborne pathogens infecting the leukocytes of many vertebrates. The finding of
Anaplasma Platys in dogs and cats has been described but the epidemiological importance of this rickettsia to the feline population
remains unknown.
Ehrlichia Canis:
Ehrlichia Canis are a group of small, gram negative, pleiomorphic, obligate intracellular cocci that infect different blood cells in various animal
species and in humans. Canine ehrlichiosis presents as a rather no specific, multisystemic disorder with the primary complaints being
depression, lethargy, mild weight loss, vomiting, diarrhea and anorexia with or without hemorrhagic tendencies.
Hepatozoon Canis:
Hepatozoon Canis is a tick borne protozoan parasite, classified in the Phylum Apicomplexa. The parasite is primarily transmitted by the tick,
Rhipicephalus sanguineus. In dogs, the infection results in the development of schizonts within various tissues and gamonts in the peripheral
blood. Hepatozoon canis infection in dogs can be associated with a distinct clinical syndrome that involves chronic myositis, debililation and
death.
Trypanosoma Evansi:
Trypanosoma Evansi is widely distributed monomorphic protozoan which infects pet and large animals such as dogs, camels, equines and
cattle. The organism also has been found infecting cats.
Trypanosoma Evansi is mechanically transmitted by blood sucking insect species. The parasite destroys red blood cells, provoking serious
and often fatal anaemia.
Not for Vetro - Legal purpose Page 3
Date 29/09/2023 Srl No. 4 Breed LABRADOR RETRIEVER
Pet Name . ESKA Age 7 Yrs. 5 Mn. 1 Day Sex F
Owner VIDYAUM RATHOR CLINIC Printed on 29/09/2023
Ref. By Dr. SHALLY Contact No.
Species CANINE
MR. ANUJ KUMAR Mr. NAVEEN LOHIA DR. FAAZIL BASHIR RATHER
LAB TECHNICIAN Lab Technician Super Specialist (Veterinary Pathology)
DMLT(GND-002/1686) BVSc & AH,MVSc
PGDAW
Not for Vetro - Legal purpose Page 4 of 4