Hypothesis on Light Intensity and Photosynthesis
Hypothesis on Light Intensity and Photosynthesis
Name: …………………………………………………………
Learning Aim A – Planning an investigation
Writing a hypothesis for investigations – A1
Hypothesise are made as a starting point for an investigation and can be changed as further
investigations are carried out and the subject is taken forward.
A null hypothesis is a prediction (where you might not expect to find a particular trend or
pattern) which states that any recorded/observed differences between two or more data sets
is due to chance. A null hypothesis is tested by analysing results statistically. If the null
hypothesis is rejected, this indicates that there is a statistically significant difference (which
can be explored/explained with science).
Precision – the precision of equipment is inversely proportional to the unit size, i.e. the
smaller the unit, the more precise it is.
(In length measurements, µm units are more precise than mm units – because 1mm =
1,000µm)
Absolute error is an accuracy equal to +/- half of the smallest unit marked on the scale of the
measuring device.
(A ruler measuring only in 1cm intervals is less accurate than a ruler measuring in 1mm
intervals – 1cm = 0.5cm or 5mm is absolute error, 1mm = 0.5mm is absolute error)
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Risk – the level of harm that could be caused by a hazard, AND, the chances of it happening
(this depends on the method).
Variables – A4
Dependent – The variable affected/changed by the independent variable and the variable
measured.
Control – The variables which need to be kept constant as they would others impact on the
dependent variable.
Confounding – The variables which will inevitably have an impact on the dependent variable
and which can’t be easily controlled.
Methods – A5
Methodologies should always be written as step-by-step instructions, in the third person past
tense.
Standard operating procedures (SOP) – these are applicable to most scientific investigations
and comes under Good laboratory practice (GLP). It includes detail on how tests are carried
out, how chemicals are handled and stored, how waste is disposed of, how equipment is used
and maintained and the use of Personal Protective Equipment (PPE).
Accuracy – the accuracy of results is based on their closeness to the true/actual values.
Reliability – the reliability of results is how trustworthy they are. This can be impacted by
their reproducibility (can someone else do the experiment and get the same results),
repeatability (can an individual do the experiment again and get the same results).
Validity – the validity of results and any conclusions drawn depends on whether the control
variables were maintained.
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Reliability and Anomalous results – the reliability of data collection depends on taking repeat
readings for each independent variable interval. If two readings are the same they are
reliable. If they are not the same, a third (repeat) reading is needed. A result which does not
lie consistently with the rest is an anomaly (an anomalous result) which can be
discarded/ignored. The results that are consistent can be used to make a reliable
mean/average.
Qualitative data – Observations are often qualitative (descriptive) and give categorical data
(where there is a set amount of possible outcomes).
Portraying results/data – B2
Tabulating data – tables are used to record:
1. Raw data (both qualitative and quantitative data can be recorded in tables but must be in
separate tables if collected together in one experiment).
2. Processed data (quantitative data that has been mathematically/statistically manipulated
Tip 1: Draw your table BEFORE you collect your raw data as you will need to fill it in as your go along
Note this column has been subdivided into 3 so that Never use mixed units (such as
all 3 repeats can be recorded. minutes & seconds for time).
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Tip 7: The mean time has been worked out by Tip 8: The mean rate has been worked out by
adding the time from the 3 repeats and dividing dividing 30 cm3 by the time taken to collect it.
by 3. Show it to the same number of decimal REMEMBER: rate is always something per unit
places as the data which was used to calculate it. time (so divide by time).
Yeast (enzyme) concentration Mean time taken to collect Mean rate of oxygen
/mg cm-3 30 cm3 of oxygen production
/s / cm3 s-1
5 33 0.91
9 25 1.20
14 18 1.67
18 14 2.14
23 12 2.50
28 12 2.50
Tip 9: In some experiments, all you record is the time taken for something to happen.
5 33 0.91
33 33 32
9 25 1.20
24 26 25
14 18 1.67
17 20 16
18 14 2.14
13 13 15
23 12 2.50
12 11 12
28 12 2.50
11 11 13
Processing results/data – B2
1. Percentage
- (Value/Total) X100
- eg: 37/100 on a test = 37%
2. Percentage change
- (Original-New value)/Original X100
(Homer Simpson! DOH! Difference/Original X Hundred
- eg: 78/100 on next test so (37-78) / 78 X100 = 110.81% increases
3. Mean
- All values added together / number of values
- 37, 42, 45, 35, 40 (added together = 199) (divided by 5 for number of values) = 39.8
4. Standard deviation
- ‘The spread of data about the mean’.
- Gives an indication of how spread out the results are
-
- S = standard deviation
- ∑ = Sum of
- X = one data/result value
- = mean/average
- n = sample size
- eg:
X = 37, 42, 45, 35, 40
= 39.8
Subtract each number by the mean and square the result
(37-39.8)2, (42-39.8)2, (45-39.8)2, (35-39.8)2, (40-39.8)2
Add up all these values
= 62.83
Divide by one less than the sample number
=62.83 / (5-1) = 15.7075
Square root this number to get the final answer of Standard Deviation
= √15.7075
= 3.96
5. Standard error
- Errors bars of standard deviation applied (± plus and minus) to the mean on a graph
- Can be used to determine if there is a scientifically significant difference between the
mean/average of two or more data sets (see more later)…
6. Chi-squared test
- Determines if there is a significant difference between expected/predicted results and
observed/actual recorded results.
-
- eg:
-
- eg:
8. Standard form
- You may be asked to demonstrate your answers to mathematical equations in standard
form.
- i.e. For decimals: 0.0005 = 5X10-4
- i.e. For large numbers: 600000 = 6X105
2. (a) For female patients aged 20-39 only, calculate the percentage who had ‘fracture of the arm bone’.
Give your answer to 2 d.p. (2 marks)
(b) For male patients of all ages, calculate the percentage who had ‘unspecified back problems’.
Give your answer to 2 d.p.
(2 marks)
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(c) In data from the previous year, there were 18 women aged 60-79 who had ‘deformed toes’.
Calculate the percentage increase in this year’s data. Give your answer to 2 d.p.
(2 marks)
3. Look at the data and draw some conclusions from it (the first one has been done for you).
a) Fractures of arm bones are most common in the 0-19 age group for males and females
b) ……………………………………………………………………………………………………………………………………..
c) ……………………………………………………………………………………………………………………………………..
d) ……………………………………………………………………………………………………………………………………..
e) …………………………………………………………………………………………………………………………………….. (4 marks)
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1 40 26
2 45 24
3 38 20
4 42 29
1 15 17
2 17 19
3 19 20
4 16 18
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You will be asked to describe trends in your own results and/or the results given to you by
another experiment.
For this you must look at the data in your results table and/or graph and describe any
relationships shown.
When describing relationships with discrete/categorical data, you will need to assess the data
given – try grouping results that are similar against those that are different.
You may be asked to use data (primary or secondary) to predict an untested result.
i.e. Results 2, 5, 8, 11. The next likely result is 14 because there is a trend of +3…
After carrying out statistical tests (standard deviation and standard error, X2 and t-test), you
may be asked to make some valid conclusions about differences between data – i.e.
determine whether any differences in results are statistically significant (which can therefore
be explained scientifically) or whether any differences in results are just due to
chance/random error.
For example:
Pigs require a mixture of fibre and protein in their food. The greater the ratio of fibre to
protein, the less the food costs.
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Scientists took five large groups of pigs. They fed each group a different high-fibre food. Each
of the foods contained fibre from different plant species, but they all had the same energy
content. The scientists fed a control group of pigs a low-fibre food with the same energy
content. After 10 days, the scientists compared the masses of the pigs fed on high-fibre food
to those fed on low-fibre food.
The graph shows the results of the investigation. The bars represent ±2 standard errors of
the mean.
A farmer saw these results and concluded that he should replace his pigs’ usual food with
food B. Evaluate this conclusion.
Template answer:
- Food B produces greater mass than the control / greater than 100%
- But error bars for B mean show that B is not significantly different from control / from 100%
- And A and B error bars overlap
- So B is not significantly better than A
- However the experiment only ran for 10 days
- There is no information about cost.
- Experimental conditions / breed of pig could be different
Answering a null hypothesis with chi-squared (X2) and critical value comparison:
When comparing the values of observed vs. expected results, X 2 can be calculated and
compared to a ‘critical value’ in order to determine if there is significant difference / chance
difference.
Critical values are obtained by looking at a table of critical values and using the one which is
specific to the context – this is based on a p-value (probability value) of 0.01-0.05 or 1-5% in
Science and a degree of freedom = n-1.
The p-value = 0.05 states that our critical value to accept chance occurring in the results is
limited to 5%. So we need to be 95% confident of any significant difference in results.
The degree of freedom is dependent on the sample size (n) – 1.
- Accept the null hypothesis (there is no significant difference / difference is due to chance) if
X2 is less than the critical value.
- Reject the null hypothesis (there is significant difference) if the X 2 is equal to/more than
the critical value.
For example:
16 woodlice were placed in a choice chamber where there are four different environmental
conditions.
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Table of χ2 probabilities
20% 10% 5% 2% 1%
Reject the null hypothesis – the X2 is greater than the Critical value, so the difference
between observed and expected is significant.
There is less than 5% probability that the difference is due to chance.
There is 95% confidence that the difference is significant.
5 4 7 6
10 15
15 12
20 16 28 33
25 35
30 24 51
35 48
A 40 6
B 30 6
C 15 8
D 60 10
2. Work out chi-squared for the data and answer the null hypothesis: “there is no
significant difference between the observed frequency and the expected frequency of
fish”.
A section of river was searched for 4 types of freshwater fish. It was expected that there
would be equal numbers of each type of fish collected – a total of 40 fish were collected.
15 were roach, 15 were perch, 6 were pike and 4 were bream.
The critical value for 3 degrees of freedom (n-1) at a p-value of 0.05 = 7.82.
Evaluations – C2
- Errors:
Mistakes in carrying out method (human error).
Inherent percentage error in the equipment (systemic error).
- Anomalous results:
An explanation of how anomalies occurred.
A description of how future anomalies can be avoided (carrying out repeats and calculating
averages).
- Improvements/Alternatives:
Modifications which would improve the reliability, accuracy or precision of results.
Modifications which would reduce/remove errors and anomalies.
- Extensions:
Additional experiments/steps in method which would give more data.
Could expand on the range and/or intervals of the current independent variable.
Could change the independent variable (swop one of the control variables to make a new
independent variable).