Bacterial Metabolism and Sewage Treatment
Bacterial Metabolism and Sewage Treatment
BACTERIAL METABOLISM
All organisms require sources of carbon, nitrogen, sulphur, phosphorous, water and trace elements.
Many organisms require compounds produced by other organisms as their source of the above elements.
These organic compounds must be broken down to their simplest units by the organisms. There are two
digestion processes: outside the cell and inside. Enzymes are biological catalysts which lower the
energy required to break down organic molecules and hence cause (expedite) biodegradation to occur
spontaneously.
Cells and microorganisms (such as bacteria) secrete enzymes which hydrolyse larger molecules to
smaller units which are readily absorbed into the cell. These smaller units are finally broken to CO 2,
water and other elements by enzymes produced within the cell.
There are two steps involved in the process of biodegradation by bacteria: transfer and conversion.
Transfer occurs when the organic food comes in contact with the bacteria. In this step food is
transferred from the water to the bacteria through two simultaneous actions: adsorption and
absorption. The diagram below shows soluble organic matter is absorbed by passing directly through
the cell wall (membrane) into the bacteria. Particulate and colloidal solids suspended in the liquid are
adsorbed onto the cell membrane. Adsorbed food matter must be broken into a simple soluble form
before it can be absorbed into the cell.
Conversion of food matter occurs after the food enters the cell. The food matter is used either for
growth or energy. During synthesis, the food is converted into new growth or bacteria. During
oxidation, the food is broken down to end products such as carbon dioxide, ammonia and water in
order to provide the bacteria with energy. Both actions are termed metabolism and metabolic activity
is the rate at which they convert food matter to growth or energy.
Biodegradation is performed by bacteria that use domestic waste as a source of food. These bacteria can
only digest soluble organic foods and hence must break down any suitable solids to a soluble form.
They do this by secreting enzymes into their environment, which break down solids to suspended solids
and then to soluble components. They then ingest these soluble organics and break them down to their
simplest forms.
Enzymes are catalysts made of protein. A catalyst is a substance, which causes a chemical reaction to
take place. Micro-organisms manufacture thousands of different enzymes. A substrate is the matter acted
upon by the enzyme (such as an insoluble organic molecule). There are many stages in completely
biodegrading a substrate to, say carbon dioxide and water. Each stage of this biodegradation process is
catalysed by a different enzyme. An enzyme acts on the shape of the substrate breaking it down to
products that have a different shape to the parent substrate. In order to completely biodegrade a waste, all
the enzymes required for each reaction must be present. As each compound is broken down by an
enzyme, the micro-organism must produce the different enzyme necessary for the subsequent reaction.
This group of enzymes working together is known as an enzyme process. If one of these enzymes is
missing in the sequence the biodegradation process stops.
While enzymes are responsible for the biodegradation process, it is micro-organisms that produce them.
Different species of micro-organism digest different classes of food and thus manufacture and secrete
different enzyme systems. The efficiency of the biodegradation process is dependent on the right
enzymes being present in adequate concentrations.
The composition of the biomass is dictated by the composition of the waste and if this waste changes its
composition the biomass adapts accordingly. This is of course restricted to within the class of waste
being treated. Each species of microorganism digests a specific range of food and thus secretes the
various complete enzymes systems required to do so.
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Microorganisms like bacteria have nutritional preferences. For example, some bacteria metabolise
protein only, and hence produce only enzymes for this purpose. Some microorganisms have a tendency
to over produce their substrate - specific enzymes. It is these microorganisms which are most useful in
wastewater treatment.
What is bioaugmentation?
Nature employs bacteria as its cleaning staff to rid the environment of wastes. The bacteria consume
these wastes as a food source and so purify the environment. Unfortunately, the modern world places a
larger load of pollution and waste on the environment than it can cope with. Bioaugmentation is the
addition of bacteria that have been specially chosen for their ability to biodegrade these wastes (e.g.
Sewage, fats, and mineral oils).
Minute solids / particles suspended in water. An indication that the solids have not completely
biodegraded within the effluent.
Suspended solids in sewage is a guide to the amount of sludge which will be produced by treatment, and
is one of the important tests by which the efficiencies of settlement tanks and other treatment units are
judged.
A measure of the concentration of organic pollutants in effluent. The COD test involves measuring the
oxygen used by organic compounds present in a effluent. A high COD or BOD effluent can cause
substantial deoxygenating of the receiving water body.
A high BOD reading in the final effluent indicates the presence of a large number of oxygen consuming
micro-organisms in the effluent entering the environment causing an acute shortage of oxygen for
aquatic life which may die from asphyxiation.
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THE SEPTIC TANK
The Septic Tank is a concrete tank that receives waste from toilets, bathrooms and kitchens. The tank has
two chambers, the primary or solids chamber and the secondary or liquid chamber. There is a French
drain or soakaway into which the liquid from the secondary chamber flows. The soakaway is the final
treatment of the effluent and will vary in size depending on the size of the tank and amount of people the
system is designed for.
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growth rate. It can take up to 3 months to re-establish a viable and strong biomass. In the interim solids
build up in the system.
Inlet Outlet
Anaerobic
Aerobic Sludge Buildup Sludge
Buildup
Primary Chamber Secondary Chamber
Buildup of
Fats and Gels French Drain
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A maintenance programme should be implemented once the system is brought under control and the
solids are broken down. This will ensure a continual introduction of new, strong and hungry bacteria that
will maintain a well-balanced biological activity and prevent any further build up.
Manholes
Aerobic Sludge
Inlet Outlet
Anaerobic
Sludge
No Build-up of
Fats and Gels, French Drain
Percolation of liquids
Sufficient biological activity breaks down the incoming effluent
The anaerobic and aerobic sludge build up is broken down and a normal anaerobic sludge and
aerobic scum will be present
The suspended solids and fat particles are reduced therefore they do not build up in the soakaway
The congestion in the soakaway is broken down, allowing for normal percolation
Varying levels of loading can be controlled by maintaining a viable biomass
Sewage from small towns or groups of buildings in remote areas is often treated in oxidation dams. An
oxidation dam system has one or many dams in series. The raw sewage usually passes through some
screens to remove large solid material before entering the first dam. The dams are shallow and the
conditions are usually aerobic so the bacteria are mostly aerobic.
The dam may be old and over time stabilised sludge naturally collects and build’s up on the bottom
of the dam
the system is overloaded, it is receiving more raw sewage/influent than it was designed for.
the system may be receiving high loads of hydrocarbons which lie in suspension on the surface of the
water, this kills off the aerobic bacteria required to biodegrade the effluent. Wastes from industry are
also harmful to the population of bacteria.
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large amounts of fats entering the system will build up on the dam forming a scum, this reduces the
ability of the sun’s UV rays to penetrate through the water preventing it from naturally lowering the
[Link] present in the dam. These fats take longer to biodegrade than sewage and will build up
reducing the surface area required to biodegrade the aerobic effluent that results in the dam becoming
anaerobic. Reducing surface area results in less evaporation of the treated waste.
Algae, Duckweed, reeds and shrubs thrive in sewage dam environments because of high
concentration of nutrients i.e. phosphates and nitrates, in the incoming sewage. These plants have a
“choking” effect on the ponds by reducing the ponds’ volume and capacity to treat the incoming
effluent.
High levels of suspended solids settle out of suspension and create a build up of anaerobic sludge.
within the dam toxic by products such as methane gas and hydrogen sulphide are produced in
anaerobic conditions and kill off the healthy aerobic bacteria, fish and other pond life.
a surface sludge develops which eventually forms a crust.
in these situations the biomass is not large enough or strong enough to biodegrade the sludge build
up, the biomass is ineffective in its ability to completely biodegrade the effluent.
the pond eventually becomes totally congested with solids and the final effluent does not meet
effluent discharge standards.
…. Methane Gas
..
Anaerobic Sludge
by applying our bioaugmentation products the dam is inoculated with a range of aerobic, anaerobic
and facultative anaerobic bacteria and enzymes which are selected for their ability to biodegrade the
old stabilised anaerobic sludge, organic anaerobic sludge, aerobic sludge and solids as well as reduce
the suspended solids present in the liquid effluent.
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if there is a low biological activity in the dam due to toxins killing off the bacteria, Pitking and
Fatking will restore the biomass and maintain high biological activity through further applications.
This will enable the natural population of bacteria to function efficiently.
Pitking will breakdown the sludge from sewage while Fatking will breakdown the fats floating in the
dam as well as any fats trapped in the sludge blanket. This will assist the dam in its ability to
biodegrade effluent aerobically and will allow UV rays to penetrate through the effluent to naturally
sterilise the effluent.
once a dam is returned to its normal capacity and the anaerobic environment is removed, aerobic
biodegradation will once again be restored.
a hydraulically overloaded dam can be controlled by applying a preventative bioaugmentation
maintenance programme, in this way large amounts of bacteria and enzymes can improve the
biodegradation efficiency per unit volume of effluent therefore a shorter retention time would be
required to treat the waste water.
in the initial stages of treatment the inorganic and organic sludge will begin to break down,
transforming much of this to suspended solids, this process releases large amounts of nutrients into
the surrounding environment. The result will be higher levels of Phosphates and Nitrates being
noticed in the final effluent, but as the dam is being rehabilitated these levels will slowly begin to
decrease. By installing aerators ammonia can be reduced. Phosphate removal can be accomplished
through precipitation. Chemicals used in practice for phosphate precipitation from waste waters
include aluminium and iron salts as well as lime. Nitrates can be used as a source of oxygen by the
Facultative Anaerobic bacteria. By lowering the nutrient levels in the dam, the Algae, Duckweed etc.
can be more easily controlled, therefore preventing “choking” of the dam.
as the anaerobic sludge is being broken down in the initial stages of the shock dose, gasses will be
released which will belch up to the surface of the dam, as this happens the anaerobic sludge will
brake free of the sludge blanket and will rise to the surface and this will result in a more concentrated
aerobic sludge layer. Once the sludge has been transformed from anaerobic to aerobic the aerobic
bacteria begin biodegrading the sludge.
in the initial stages high levels of suspended solids will also be noticed as the solid sludge begins to biodegrade. The
bacteria and enzymes break solids into suspended solids and then into soluble organics in order for the bacteria to absorb
this and convert it into final products such as carbon dioxide and water.
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THE SEWAGE TREATMENT PLANT
The purpose of sewage treatment prior to disposal by dilution is to remove from the sewage enough
solids to permit the remainder to be discharged to receiving waters without interfering with its best or
proper use, taking into consideration the capacity of the receiving waters to assimilate the added load.
The solids that are removed are primarily organic but include also inorganic solids. Treatment must be
provided for the solids and liquids that are removed as sludge, and treatment to control odours, to retard
biological activity or destroy pathogenic organisms is also needed.
While the devices used in sewage treatment are numerous they may all be included under five methods.
1. Preliminary treatment
2. Primary treatment
3. Secondary treatment
4. Chlorination
5. Sludge treatment
1. Preliminary Treatment
At most plants preliminary treatment is used to protect pumping equipment and facilitate subsequent
treatment processes. Preliminary devices are designed to remove or cut up the larger suspended and
floating solids: to remove the heavy inorganic solids: and to remove excessive amounts of oils or
greases.
To effect the objective of preliminary treatment, the following devices are commonly used:
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2. Shredding devices – grinders, cutters, shredders
3. Grit chambers
4. Pre-aeration tanks (is accomplished by forcing compressed air into the sewage for a period of
20 to 30 minutes at the design flow).
1. remove or to reduce in size the large suspended or floating organic solids. These solids consist of
pieces of wood, cloth, paper, and garbage, together with some faecal matter.
2. Remove heavy inorganic solids such as sand, gravel and possible metallic objects, all of which
are called, grit.
3. Remove excessive amounts of oils or greases.
2. Primary Treatment
By this treatment most of the settable solids or about 40 to 60 percent of the suspended solids are
separated or removed from the sewage by the physical process of sedimentation in settling tanks.
Approximately 25 to 35 per cent of the BOD is also removed. The purpose of primary devices is to
reduce the velocity of the sewage sufficiently to permit solids to settle. In primary treatment the velocity
of flow is reduced to a fraction of an inch per second in a settling or sedimentation tank for sufficient
time to allow the major portion of the settable solids, which are largely organic, to settle out of the
sewage flow. Principle primary treatment devices are sedimentation tanks, some of which have the
further function of providing for the decomposition of the settled organic solids, known as sludge
digestion. Because of variations in design and operation, settling tanks can be divided into four general
groups.
In many cases, primary treatment is adequate to permit the discharge of the effluent to the receiving
waters.
3. Secondary Treatment
This must be used where sewage after primary treatment still contains more organic solids in suspension
or solution than the receiving waters can assimilate without infringing upon their normal proper use.
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Secondary treatment depends primarily upon biological aerobic organisms for the biochemical
decomposition of organic solids to inorganic or stable organic solids. If the primary treatment is not
sufficient there are two basic methods of secondary treatment available, trickling filters (biofilters) and
activated sludge. Sand filters can be used when a high degree of treatment or a polishing effect is
required. There are several other methods that are used to a limited extent. Theses types of treatment
employ biological growths to effect aerobic decomposition or oxidation of organic material into more
stable compounds and provide for a higher degree of treatment than that accomplished by primary
sedimentation alone.
While trickling filters and activated sludge both depend on aerobic biological organisms to effect
decomposition, there is an operational difference. In filters the organisms are attached to the filter
medium (rocks) and the organic material on which they do their work is brought to them. With activated
sludge, however, the organisms are migrant and are carried to the organic matter in the sewage. In either
case, successful operation involves the maintenance of aerobic environmental conditions favourable for
the life cycle of the organisms, and control over the amount of organic matter which they decompose.
The organic matter is the food upon which these organisms live. If they are either over-fed or under–fed
their efficiency is reduced.
The devices used in secondary treatment may be divided into four groups.
1. Tricking filters/Biofilters (consists of three parts, (a) the bed of filter medium, (b) an
underdrainage system, and (c) mechanism for distributing the sewage evenly over the surface of
the filter) – with secondary settling tanks. They occupy large areas and are expensive to
construct. Ponding is always an indication that something is wrong, and if there is no flow
through the underdrainage system in certain areas this is an indication of congestion in the bed of
filter medium. Since these filters do not remove all the suspended solids a secondary settling tank
is required to settle out these suspended solids.
2. Aeration tanks – (a) activated sludge with final settling tanks, and, (b) contact aeration.
Activated Sludge
The development of the activated sludge process marked an important advance in the secondary
treatment of sewage. Like the trickling filter, it is a biological contact process where living
aerobic organisms and the organic solids in the sewage are brought together in an environment
favourable for the aerobic decomposition of the solids. Since the environment is the sewage
itself, efficient operation of the process is dependent on the continual maintenance of dissolved
oxygen at all times throughout the sewage being treated. The environment itself, however,
accomplishes little unless enough living workers inhabit it.
Normal sewage contains some of these biological workers but the number is far too small to do
the work at hand. It is necessary, therefore to add many more organisms and to distribute them
throughout the sewage before the activated sludge process can begin to function with any
efficiency.
The activated sludge process is usually employed following plain sedimentation. The sewage
contains some suspended and colloidal solids and when agitated in the presence of air, the
suspended solids form nuclei on which biological life develop and gradually build up to larger
solids which are known as activated sludge. Activated sludge is a brownish floc-like substance
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consisting largely of organic matter obtained from the sewage, and inhabited by myriads of
bacteria and other forms of biological life. Activated sludge with its living organisms has the
property of absorbing or adsorbing colloidal and dissolved organic matter including ammonia
from sewage so that the suspended solids are reduced. The biologic organisms utilise the
absorbed material as food and convert it into insoluble non-putrescible (not liable to rot) solids.
Much of this conversion is a step-by-step process. Some bacteria attack the original complex
substances to produce simpler compounds as their waste products. Other bacteria use these waste
products to produce still simpler compounds and the process continues until the final waste
products can no longer be used as food for bacteria.
The generation of activated sludge or floc in sewage is a slow process, and the amount formed
from any volume of sewage during its period of treatment is small and inadequate for the rapid
and effective treatment of the sewage, which requires large concentrations of activated sludge.
Such concentrations are built up by collecting the sludge produced from each volume of sewage
treated and re-using it in the treatment of subsequent sewage flows. The sludge so re-used is
known as returned sludge. This is a cumulative process so that eventually more sludge has been
produced than is needed for re-use. The surplus, or excess activated sludge, is then permanently
removed from the treatment process. The activated sludge must be kept in suspension during its
period of contact with the sewage being treated by some method of agitation. The activated
sludge process, therefore, consists of the following steps.
The most common problem encountered in the operation of activated sludge plants is sludge
bulking. A desirable activated sludge is one, which settles rapidly leaving a clear, odourless and
stable supernatant. The floc is granular in appearance, with sharp defined edges, is golden drown
in colour and has a musty odour. When the character of the activated sludge changes so that its
settleability decreases, as measured by a significant rise in the sludge volume index, a condition
known as sludge bulking develops in the final settling tank. As a portion of the sludge does not
settle in the tank, it is carried away in the effluent. This results in a serious impairment in the
quality of the plant effluent and places an added organic load on the receiving waters.
There are a number of causes for sludge bulking, some of which are given below.
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All of these can be pretty well summed up by saying that sludge bulking results from overloading
or the improper balance between the tree variable – BOD loading, suspended solids concentration
of the mixed liquor, and the amount of air used in aeration.
Contact aeration
A typical contact aeration plant consists of five tanks in series to provide for primary settling,
first stage aeration, intermediate settling, second stage aeration and final settling. In addition,
facilities for sludge treatment and disposal must be provided.
3. Intermittent sand filters – bed of sand on which effluent from primary treatment or from
trickling filter or secondary settling tanks may be applied intermittently by using troughs or
perforated pipe distributors. The effluent from the filter is removed by an underdrainage system.
4. Stabilisation ponds – the process involves two steps in the decomposition of organic matter in
sewage. The carbonaceous matter in sewage is first broken down by the aerobic organisms with
the formation of carbon dioxide. Algae during photosynthesis use the carbon dioxide that is
formed. Photosynthesis is a natural process carried on in green plant tissue under the influence of
light and in the presence of chlorophyll, which is the green colouring substance of plant life. In
this process, the oxygen in the carbon dioxide is liberated and dissolved by the liquid in which
the algae grow. As a result the organic matter in sewage is converted into algae and the sewage is
supplied with oxygen to support further aerobic decomposition. The sewage solids enter the pond
in a highly putrescible state and leave in the form of highly stable algae cells which, within
certain limitations, can be discharged to receiving waters without deleterious effect.
Oxidation ponds can be used as a complete treatment receiving raw sewage, or as a secondary
treatment for settled sewage, or as a further treatment for effluents from secondary processes.
They have been used most commonly as a secondary treatment for primary effluents.
4. Chlorination
This is a method of treatment, which may be employed for many purposes in all stages in sewage
treatment, and even prior to preliminary treatment. It involves the application of chlorine to the
sewage for the following purposes.
Sewage sludge is the mixture of sewage and settled solids. Descriptive of its source, it may be termed
primary, secondary, excess activated, or chemical sludge. From its state, or treatment received, it may be
called raw or fresh, digested, dewatered or dried. These are the most common descriptive terms and may
be used in combination. Other descriptive terms are Imhoff and septic tank sludge.
The sludge removed from sewage in both primary and secondary treatment units, together with the water
removed with them, constitute sewage sludge. While in some few instances satisfactory disposal without
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treatment is practical, it is generally necessary to subject sludge to some treatment to prepare or
condition it for disposal without creating unsatisfactory conditions. Such treatment has two objectives –
the removal of part or all of the water in the sludge to greatly reduce its volume and the decomposition
of the putrescible organic solids to mineral solids or to relatively stable organic solids. This is
accomplished by a combination of two or more of the following methods.
1. Thickening – This process involves the concentration of thin sludges to more dense sludge in
special tanks designed for this purpose. The thickening tank is equipped with slowly moving
vertical paddles built like a picket fence.
2. Digestion with or without heat – the purpose of digestion is to attain both of the objectives of
sludge treatment. Sludge digestion is carried out in the absence of free oxygen by anaerobic
organisms. It is, therefore, anaerobic decomposition. The solid matter in raw sludge is about 70
per cent organic and 30 per cent inorganic or mineral. Much of the water in sewage sludge is
“bound” water which will not separate from the sludge solids. The living organisms breakdown
the complex molecular structure of these solids setting free the “bound” water and obtaining
oxygen and food for their growth. The digestion process is seldom carried to full completion but
only to a degree where the resulting solids, although still organic in composition, have been
sufficiently decomposed to become relatively stable or inert, in the nature of humus material.
Since digestion is accomplished by living organisms, it is desirable to provide an environment in
which they are most active and carry on their work in the shortest time. The environmental
factors involved are moisture, temperature, availability of proper food supply, and alkalinity. To
these might be added the absence of chemicals toxic to the organisms.
3. Drying on sand bed – open or covered – it is a device to remove enough water so that the
remainder can be handled as solid material with moisture content below 70 per cent.
4. Conditioning with chemicals
5. Elutriation (to purify by washing)
6. Vacuum filtration
7. Heat drying
8. Incineration
9. Wet oxidation
10. Floatation with chemicals and air
11. Centrifuging
Sludge disposal
All the sludge produced at a treatment plant must be disposed of ultimately. Treatment processes such as
have been described may reduce its volume or so change its character as to facilitate its disposal, but still
leave a residue which in most cases must be removed from the plant site. Like the liquid effluent from
the treatment plant, there are two broad methods for the disposal of sludge – (1) disposal in water, and
(2) disposal on land. This applies regardless of whether or not the sludge is treated to facilitate or permit
the selected method of disposal.
Disposal in water – this is an economical but not common method because it is contingent on the
availability of bodies of water adequate to permit it. At some seacoast cities sludge either raw or digested
is pumped to barges and carried to sea to be dumped in deep water far enough off shore to provide huge
dilution factors and prevent any ill effects along shore.
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Disposal on land – under land disposal the following may be included:
1. Burial
2. Fill
3. Application as fertiliser or soil conditioner.
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STEP 1:
The Sewer Outfall
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5.3. SEWAGE TREATMENT PLANT
KEY :
STEP 6 : Disinfection
Adding chlorine or bromine disinfects effluent – or a more environmentally friendly product
is the UV (ultra violet) filter.
STEP 7 : Maturation
Effluent is stored for up to 24 hours to allow improvement in the effluent.
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