Overview of Volatile Oils and Their Uses
Overview of Volatile Oils and Their Uses
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Content
Contd…
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Introduction
• Volatile oils are the complex mixture of hydrocarbon terpenes, mono &
sesquiterpenes and polyterpenes and their oxygenated derivatives.
• They are extracted from various parts of the medicinal plants and have
medicinally active constituents responsible for therapeutic activities.
• They get evaporated on exposure to air and responsible for the odour or
essence of the plant, hence also called essential oil or aromatics.
• Some contain volatile alkaloids (e.g. Valerotropes) and some are found
linked with resins & gums.
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Introduction
Contd…
• Most of the fragrance component of plant are volatile and isolated by steam
distillation is called as essential oil.
• Due to their pleasant smelling nature the mono and sesquiterpenoids are of
considerable commercial importance particularly in perfumery.
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Introduction
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Introduction
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Occurrence & Distribution
Occurrence of Volatile oils:
The volatile oils are distributed in different parts of the plants as shown in
the table.
S. No. Plant part Distribution
1 Leaves Mint (mīniti)
2 Bark Cinnamon (k’erefa)
3 Fruits Coriander (Dimbelal), Fennel
(Ensilal), Caraway
4 Flower bud Clove (k’irinifudi)
5 Lysogenous and schizogenous Plants belonging to the family
cavities Rutaceae
6 Glandular hairs Plants belonging to the family
Labiatae
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Therapeutic Uses of Volatile Oil
• Volatile oils are used as carminatives e.g. cinnamon (k’erefa).
• They have antiseptic, antibacterial and antifungal activities, e.g. eucalyptus
oil.
• They are used in the treatment of cough and asthma, e.g. clove oil.
• They have analgesic and anti-inflammatory actions.
• They are used in aroma therapy.
• They are used as diuretics, e.g. juniper oil.
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Physical Properties of Volatile Oil
• Volatile oils are colorless liquids.
• They are lighter than water.
• They possess distinct odor.
• They are optically active in nature.
• They are insoluble in water and soluble in organic solvents.
• They can be extracted from entire plant or any part of the plant.
• Volatile oil should be stored in tightly closed amber colored bottles in a
cool, dry place as they become darker in color, especially when exposed to
air and direct sunlight.
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Classification of terpenoids
• Most natural terpenoids hydrocarbon have the general formula (C5H8)n.
• They can be classified on the basis of value of ‘n’ i.e., isoprene unit or
number of carbon atoms present in the structure.
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Chemical properties
Terpenoids are the hydrocarbones of plant origin of the general formula
(C5H8)n as well as their oxygenated, hydrogenated & dehydrogenated
derivatives.
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On thermal decomposition, terpenoid gives isoprene as one of the product
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Biosynthesis of Volatile Oil
• In aromatic plants species, biosynthesis of essential oils occurs through two
complex natural biochemical pathways involving different enzymatic
reactions.
• Essential oils are final terpenoid products and are formed by a huge group
of enzymes known as terpene synthases (TPS).
• Essential oils are important secondary metabolites of plants and have been
used in ethnobotanical medicines.
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Extraction of volatile oils
Volatile oils or essential oils are extracted by various
method:
1. Expression
2. Steam distillation
3. Extraction by use of volatile solvents
4. Enzymatic hydrolysis
5. Adsorption in purified fat
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Extraction of volatile oils
Contd…
1. Expression:
• It is not in use but possess only historical importance.
• Plant material is cut into small pieces then crushed and
screened to get juice.
• The juice is centrifuged at high speed.
• Inferior quality of oil is obtained by distillation.
• Example: Citrus fruits oil and grass oil
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Extraction of volatile oils
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Extraction of volatile oils
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2. Hydro/Steam Distillation:
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Extraction of volatile oils
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Extraction of volatile oils
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Extraction of volatile oils
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Demerits of steam distillation:
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Extraction of volatile oils
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3. Extraction by volatile solvent:
• Delicate flowers such as rose, jasmine and orange blossom are oftentimes
extracted as absolutes to yield a higher percentage of oil without using the
heat that can damage the petals during steam distillation.
• Like essential oils, absolutes come from volatile, aromatic fluids extracted
from plants.
• “Absolutes differ from essential oils in that they contain a higher density of
colorants, waxes and other constituents from the plant, making the aroma
highly concentrated and more true to nature”.
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Extraction of volatile oils
Contd…
• They are solvent extracted, and since a trace amount of solvent may be
present they are not used for therapeutic purposes, but instead, for natural
perfumery.
• The multi-step process includes first extracting the aromatic oil from the
plant material with a solvent such as hexane.
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Extraction of volatile oils
Contd…
• After the hexane is removed what is left behind is a waxy substance called
a concrete.
• The aromatic oils are then extracted from the concrete with ethyl alcohol,
and after the ethyl alcohol is removed, the remaining substance is an
absolute – an oil with an aroma close to the plant from which it came.
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Extraction of volatile oils
Contd…
4. Enzymatic Hydrolysis method:
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Extraction of volatile oils
Contd…
5. Adsorption in purified fat:
Enfleurage:
• Fat is taken in glass plate warmed to about 50ºC.
• Surface is covered with petals and kept for 24 hours.
• Replace the petals until the fat is saturated with essential oil .
• The petals are removed and the fat is digested with ethyl alcohol.
• Fat is soluble in ethyl alcohol.
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Extraction of volatile oils
Contd…
• Fat in essential oil during digestion can be removed by cooling to 20⁰C
where the fat separates out.
• Recently the fat has been replaced by coconut charcoal due to its greater
stability and higher adsorption.
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Extraction of volatile oils
Contd…
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Constituents of Volatile Oil
1. Hydrocarbons:
Terpene Structure Biological Source
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Constituents of Volatile Oil
Contd…
4. Ketones:
Thujone
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Constituents of Volatile Oil
Contd…
5. Esters:
Menthyl acetate
Rosmarinus officinalis
(Rosemary)
Bornyl acetate
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Constituents of Volatile Oil
Contd…
6. Phenols:
Thymol
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Constituents of Volatile Oil
Contd…
7. Phenolic ethers:
Eucalyptus globulus
(Eucalyptus)
Cineol
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Constituents of Volatile Oil
Contd…
8. Peroxides:
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Constituents of Volatile Oil
Contd…
8. Sulfur containing compound:
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Constituents of Volatile Oil
Contd…
8. Nitrogen containing compound:
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Lemon peel
Common Names:
English: Lemon
Amheric: Lomi
Biological Source:
Lemon oil is obtained from fresh ripe fruit peel of
Citrus lemon belonging to the family Rutaceae.
It is a volatile oil obtained by cold expression
method.
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Lemon peel
Contd…
Taxonomy:
Kingdom: Plantae
Order: Sapindales
Family: Rutaceae
Genus: Citrus
Species: C. Limon
Organoleptic Properties:
Color: Pale greenish yellow
Odor: Strong and aromatic
Taste: Aromatic and bitter
Solubility: Soluble in alcohol and insoluble in water
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Lemon peel
Contd…
Chemical Constituents:
Lemon peel contains volatile oil (2.5%), vitamin C, hesperidin and other
flavone glycosides, mucilage, pectin and calcium oxalate.
The important constituents of the volatile oil are limonene (90%), citronellal,
geranyl acetate, α-pinene, camphene, linalool, terpineol, methyl
heptenone, octyl and nonyl aldehydes, γ-terpinene, β-pinene, neral, and
geranial.
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Lemon peel
Contd…
Uses:
Lemon oil is used as carminative and stimulant.
The Juice of the fresh fruit is used as a source of citric acid and Vitamin C
concentrates.
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Coriander
Common Names:
English: Coriander
Amheric: Dimbilaala
Botanical Name: Coriandrum sativum
Biological Source:
The drug consists of dried ripe fruits of
Coriandrum sativum Linn. belonging to
family Apiaceae.
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Coriander
Contd…
Taxonomy:
Kingdom: Plantae
Order: Apiales
Family: Apiaceae
Genus: Coriandrum
Species: C. Sativum
Organoleptic Properties:
Color: Yellowish brown to brown
Odor: Aromatic
Taste: Spicy and characteristic
Size: Fruits are 2 to 4 mm in diameter and 4 to 30
mm in length
Shape: Sub-globular cremocarpous fruits
Note: Each mericarp has five wavy primary ridges alternating with
four straight prominent secondary ridges
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Coriander
Contd…
Chemical Constituents:
Coriander consist of about 1% of volatile oil the chief volatile components are
D-(+)-linalool (coriandrol), along with other constituents like, borneol, p-
cymene, camphor, geraniol, limonene, and alpha-pinenes.
The fruits also contain fatty oil and hydroxycoumarins.
The fatty oils include acids of petroselic acid, oleic acid, linolenic acid,
whereas the hydroxycoumarins include the umbelliferone and scopoletine.
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Coriander
Contd…
Uses:
• Coriander fruits are used as aromatic, carminative, stimulant,
antispasmodic, flavouring agent.
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Eucalyptus oil
Common name:
English: Fever tree, Blue gum tree
Amharic: Nech bahir Zaf
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Eucalyptus oil
Contd…
Taxonomy:
Kingdom: Plantae
Order: Myrtales
Family: Myrtaceae
Genus: Eucalyptus
Species: E. globulus
Description:
Color: Colorless or straw colored fluid
Odor: Characteristic
Taste: Characteristic
Solubility: soluble in equal amount of alcohol
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Eucalyptus oil
Contd…
Chemical Constituents:
Eucalyptus oil contains volatile oil of which 70 to 85% is 1,8-cineole also
known as eucalyptol.
The other constituents present are p-cymene, α-pinene; small quantity of
sesquiterpenes like ledol, aromadendrene; aldehydes, ketones, and alcohols.
It also has polyphenolic acids like ferulic acid, caffeic acid, gallic acid;
flavonoids such as eucalyptin, hyperoside and rutin.
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Eucalyptus oil
Contd…
Uses:
The oil is used as stimulant, antiseptic, flavoring agent, aromatic, deodorant,
expectorant, antimicrobial, febrifuge, diuretic, and antispasmodic.
It is also used in the treatment of lung diseases, sore throat, cold, as a vapour
bath for asthma and various respiratory ailments and in bronchitis.
Marketed Preparation:
It is one of the ingredients of the preparations known as Cold Balm, Muscle
and Joint Rub.
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Mint (Peppermint) Oil
Common name:
English: Peppermint, Brandy mint
Amheric: Nanna
Biological source:
It is the oil obtained by the distillation of Mentha
piperita herb, belonging to family Lamiaceae
(Labiatae).
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Mint (Peppermint) Oil
Contd…
Taxonomy:
Kingdom: Plantae
Order: Lamiales
Family: Lamiaceae
Genus: Mentha
Species: M. piperita
Description:
Color: Colorless, yellowish or greenish liquid
Odor: Characteristic and penetrating
Taste: First hot and aromatic afterwards converted to cold sensation in
mouth
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Mint (Peppermint) Oil
Contd…
Chemical constituents:
The chief constituent of Peppermint oil is Menthol, along with other
constituents like menthyl acetate, isovalerate, menthone, cineol, inactive
pinene, limonene, and other less important bodies.
The flavouring properties of the oil are due to both the ester and alcoholic
constituents.
Medicinal value is attributed only due to the alcoholic components.
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Mint (Peppermint) Oil
Contd…
Uses:
It is stimulant, stomachic, carminative, inflatulence, and colic; in some
dyspepsia, sudden pains, for cramp in the abdomen and also in cholera and
diarrhoea.
Peppermint is good to aid in raising internal heat and inducing perspiration.
It is also used in cases of hysteria and nervous disorders.
Adulterants:
Camphor oil
Cedarwood oil
Oil of African Copaiba
Rectified spirit
Rosemary oil
Turpentine oil
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Cinnamon
Common Names:
English: Cortex cinnamoni, Ceylon cinnamon,
Saigon cinnamon, Chinese cassia
Amharic: Karafa or Qarafa
Biological source:
Cinnamon is the dried inner bark of the coppiced
shoots of Cinnamomum zeylanicum Nees.,
belonging to family Lauraceae.
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Cinnamon
Contd…
Taxonomy:
Kingdom: Plantae
Order: Laurales
Family: Lauraceae
Genus: Cinnamomum
Species: C. zeylanicum
Description:
Color: Outer surface has yellowish brown color; inner surface is
darker than the outer
Odor: Fragrant aromatic and characteristic
Taste: Aromatic and sweet
Shape: Quill shape
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Cinnamon
Contd…
Chemical Constituents:
Cinnamon contains about 10% of volatile oil, tannin, mucilage, calcium
oxalate and sugar.
Volatile oil contains 50 to 65% cinnamic aldehyde, along with 5 to 10%
eugenol, terpene hydrocarbons and small quantities of ketones and alcohols.
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Cinnamon
Contd…
Uses:
It is used as an aromatic, carminative, flavouring agent, analgesic, antiseptic,
antirheumatic, antispasmodic, demulcent, digestive, expectorant, stomachic,
diaphoretic, antibacterial, antifungal, etc.
It stops vomiting, relieves flatulence and and as astringents for diarrhoea and
haemorrhage of the womb.
It is also used in the treatment of bronchitis, colds, nausea, congestion, and
liver problems.
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Dill
Common names:
English: Fructus anethi, Anethum, European dill
Amharic: Enselale
Biological source:
Dill consists of the dried ripe fruits of Anethum
graveolens Linn., belonging to family
Umbelliferae.
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Dill
Contd…
Taxonomy:
Kingdom: Plantae
Order: Apiales
Family: Apiaceae
Genus: Anethum
Species: A. graveolens
Description:
Color: Yellowish or slightly brown
Odor: Pleasant aromatic
Taste: Aromatic and characteristic
Shape: Oval, compressed, winged
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Dill
Contd…
Chemical Constituents:
The fruit yields about 3.5% of the essential oil, about 20% of fixed oil and
protein.
The essential oil is an aromatic liquid consisting of a mixture of paraffin
hydrocarbon and 40 to 60% of d-carvone along with D-limonene and other
terpenes.
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Dill
Contd…
Uses:
Dill fruit and oil of Dill possess stimulant, aromatic,
carminative, and stomachic, with considerable medicinal
value.
Oil of Dill is used in mixtures, preparation of Dill Water is
used in the flatulence of infants and also as a vehicle for
children’s medicine.
Oil of Dill is employed for perfuming soaps.
Marketed Products:
It is one of the ingredients of the preparations known as
Woodward’s Gripe water.
Common Names:
English: Clove buds, Clove flowers
Amharic: Qerenefud, k’irinifudi
Biological Source:
Clove consists of the dried flower buds of
Eugenia caryophyllus Thumb., belonging to
family Myrtaceae.
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Clove
Contd…
Taxonomy:
Kingdom: Plantae
Order: Myrtales
Family: Myrtaceae
Genus: Syzygium
Species: S. aromaticum
Description:
Color: Reddish-brown
Odor: Strong spicy and aromatic
Tatse: Pungent and aromatic
Shape: Sub-cylindrical with upper
crown and a hypanthium
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Clove
Contd…
Chemical Constituents:
Clove contains 14–21% of volatile oil.
The other constituents present are the eugenol, acetyl eugenol, gallotannic
acid, and two crystalline principles; α- and β- caryophyllenes, methyl furfural,
gum, resin, and fibre.
Clove oil has 60–90% eugenol, which is the cause of its anesthetic and
antiseptic properties.
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Clove
Contd…
Chemical tests:
To a thick section through hypanthium of clove add 50% potassium hydroxide
solution; it produces needle-shaped crystals of potassium eugenate.
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Clove
Contd…
Uses:
Clove is used as an antiseptic, stimulant, carminative, aromatic, and as a
flavouring agent.
It is also used as antiemetic.
Dentists use clove oil as an oral anesthetic and to disinfect the root canals.
Clove kills intestinal parasites and exhibits broad antimicrobial properties
against fungi and bacteria and so it is used in the treatment of diarrhea,
intestinal worms, and other digestive ailments.
Clove oil can stop toothache.
A few drops of the oil in water will stop vomiting, eating cloves is said to be
aphrodisiac.
Clove is also a stimulating expectorant in bronchial problems.
Eugenol is also used as local anaesthetic in small doses.
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Ajowan
Common Names:
English: Ethiopian cumin seeds
Amharic: Carom, Ajwain or Ajowan
Biological Source:
Ajowan is the dried ripe seeds of
Trachyspermum ammi (L.) Sprague,
belonging to family Apiaceae.
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Ajowan
Contd…
Taxonomy:
Kingdom: Plantae
Order: Apiales
Family: Apiaceae
Genus: Trachyspermum
Species: T. Ammi
Description:
Color: Dirty yellow to yellowish brown
Odor: Agreeable
Taste: Aromatic and warming
Shape: Cremocarps
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Ajowan
Contd…
Chemical Constituents:
Ajowan contains an essential oil (2–3.5%), protein (17.1%), and fat (21.8%).
Ajowan oil is a colourless or brownish yellow liquid possessing a
characteristic odour of thymol and a sharp taste.
The principal constituents of the oil are phenol, mainly thymol (35–60%),
carvacrol, p-cymene, γ-terpinene, α-, and β-pinenes and dipentene.
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Ajowan
Contd…
Uses:
Ajowan is widely used as a spice in curries; in pickles, certain types of
biscuits, confectionery, and in beverages.
It is valued for its antispasmodic, stimulant, tonic, and carminative properties.
It is given in flatulence, atonic dyspepsia, diarrhoea, and cholera.
Ajowan is also effective in relaxed sore throat and in bronchitis, and often
constitutes an ingredient of cough mixture.
The oil is useful as an expectorant in emphysema, bronchial pneumonia and
some other respiratory ailments.
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Chenopodium Oil
Common Names:
English: Herba sancti mariae, Jesuit’s tea,
Mexican tea
Amharic: sīnopodīyemi zeyiti
Biological Source:
Chenopodium oil is the volatile oil obtained by
the distillation from the fresh aerial parts of
Chenopodium ambrosioides Linn, belonging to
family Chenopodiaceae.
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Chenopodium Oil
Contd…
Taxonomy:
Kingdom: Plantae
Order: Caryophyllales
Family: Amaranthaceae
Genus: Chenopodium
Species: C. ambrosioides
Description:
Color: Colorless or yellowish
Odor: Camphoraceous
Taste: Pungent and bitter
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Chenopodium Oil
Contd…
Chemical Constituents:
Ascaridole, a terpene peroxide, to the high percentage of 60 to 70%, an
unstable substance is present in the oil.
It also contains p-cymene, α-perpinene, probably dihydro-p-cymene, and
possibly sylvestrene.
Betzine, choline, glycol, and safrol have also been reported.
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Chenopodium Oil
Contd…
Uses:
Chenopodium oil is used as anthelmintic especially in tapeworm, round
worms, and hook worms.
It is also used as active purgative, in the treatment of malaria, hysteria, and
other nervous diseases.
It is employed in veterinary practice in a worm mixture for dogs, in
combination with oil of turpentine, oil of aniseed, castor oil, and olive oil.
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