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B.Sc Nursing Microbiology Exam Paper 2023

The document discusses topics related to applied microbiology and infection control including safety. It contains multiple choice questions, essay questions and short note questions. The questions cover topics such as microbiology organisms, modes of transmission of diseases, laboratory diagnosis techniques, infection control practices like biomedical waste management and standard precautions, vaccination schedules, sterilization techniques and hospital infection control committees.

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0% found this document useful (0 votes)
707 views2 pages

B.Sc Nursing Microbiology Exam Paper 2023

The document discusses topics related to applied microbiology and infection control including safety. It contains multiple choice questions, essay questions and short note questions. The questions cover topics such as microbiology organisms, modes of transmission of diseases, laboratory diagnosis techniques, infection control practices like biomedical waste management and standard precautions, vaccination schedules, sterilization techniques and hospital infection control committees.

Uploaded by

Hiba V.A
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Reg. No: …………………...

Third Semester [Link] Nursing Degree Regular Examinations August 2023


Paper I – Applied Microbiology and Infection Control including Safety
Time: 3 Hrs Total Marks: 75
 Answer all questions to the point neatly and legibly • Do not leave any blank pages between answers
 Indicate the question number correctly for the answer in the margin space • Answer all parts of a single
question together
 Leave sufficient space between answers • Draw table/diagrams/flow charts wherever necessary
 Write section A and section B in separate answer books (32 Pages). Do not mix up questions from section
A and section B.
Section A – Applied Microbiology
Q.P. CODE: 321010 Max. Marks: 37

Multiple Choice Questions (6x1=6)


(Choose the most appropriate answer from the options given)
1. The father of antiseptic surgery is
a) Louis Pasteur b) Edward Jenner c) Robert Koch d) Joseph Lister
2. Zeihl-neelsen staining is used to demonstrate
a) gram positive bacteria b) Anaerobic bacteria
c) Candia fungi d) Acid fast bacteria
3. Zoonotic diseases are
a) Diseases spread from animals to man b) Diseases spread by plants
c) Diseases spread by contact d) Diseases spread by air
4. Natural passive immunity is
a) Antibodies transferred to baby by readymade antibody
b) Antibodies transferred through injection
c) Antibodies transferred from mother to baby by breast milk at the time of birth
d) It’s a type of innate immunity
5. Identify correct group of organisms causing dysentery
a) Salmonella and E coli b) Shigella and entamoeba histolytica
c) Vibrio cholera and pseudomonas d) Staphylococcus and shigella
6. Pandemic disease which caused pneumonia and rapid mortality in recent past is
a) Influenza virus b) COVID 19 virus
c) Corona group of virus d) Hepatitis B virus
Essay (1x10=10)
7. Draw a neat labelled diagram of HIV virus. Discuss the modes of transmission and
laboratory diagnosis of HIV infection (3+2+5)
Short Notes (3x5=15)
8. Define and classify immunity and brief innate immunity
9. Discuss urinary tract infections, brief the sample collection
10. Laboratory diagnosis of dermatophyte infection (fungal infection of skin, hair and nail)
Difference between the following (3x2=6)
11. Dengue virus vs HIV virus
12. Diarrhea vs Dysentery
13. IgG vs IgM
(PTO)
Section B – Infection Control Including Safety
Q.P. CODE: 322010 Max. Marks: 38

Multiple Choice Questions (7x1=7)


(choose the most appropriate answer from the options given)
1. In biomedical waste management, white colour coded bins used for
a) Contaminated plastic waste b) Human anatomical waste
c) Waste sharps including metals d) chemical liquid waste
2. Spaulding classification is used to
a) Classify level of disinfectant
b) Classify article to keep inside autoclave
c) Its style of hypersensitive classification
d) Its classification of salmonella in the microbiology laboratory
3. All of the following are used to identify HIV except
a) Western blot test b) Immuno-comb test
c) Real time PCR d) P26 antigen
4. MMR vaccine is used for
a) Measles and Mumps b) Mumps and Rubella
c) A and B are correct d) None of the above
5. The following are antibiotic sensitivity techniques, except
a) Kirby Bauer technique b) Stokes technique
c) Epsilometer test d) Agglutination technique
6. BCG vaccine is scheduled at the age of
a) Immediately after birth b) At the age of 9 months
c) At the age of 12 months d) At the age of 5 years
7. Most common organism associated with burns wound infection
a) Entamoeba b) Pseudomonas c) Salmonella d) Rabies

Essay (1x10=10)
8. Describe in detail the various components of standard precautions
Short notes (3x5=15)
9. Antimicrobial Stewardship programme
10. Mention the type of sample to be collected in extra pulmonary tuberculosis
11. Describe the principle, working and items sterilized in an autoclave
List the following (3x2=6)
12. Four measures to prevent DVT (Deep Vein Thrombosis)
13. Steps of Donning
14. Four members of Hospital Infection Control Committee

**********************************

Common questions

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Standard precautions include hand hygiene, use of personal protective equipment (PPE) like gloves and masks, safe injection practices, and proper handling of potentially contaminated equipment. These measures are crucial in preventing the spread of infections in healthcare settings by minimizing the risk of pathogen transmission .

Joseph Lister's antiseptic techniques revolutionized surgical practices by drastically reducing infection rates, which was a leading cause of post-operative mortality. His methods are foundational in current sterile techniques, including rigorous sterilization of equipment and maintaining sterile environments, which are crucial for preventing nosocomial infections in modern healthcare settings .

Diarrhea is characterized by frequent loose or liquid bowel movements and can be caused by various factors including viral, bacterial infections, or food intolerances. Dysentery, on the other hand, specifically involves inflammation of the intestines, often resulting in bloody stools and is commonly caused by Shigella bacteria or Entamoeba histolytica .

Natural passive immunity is acquired through the transfer of antibodies from mother to baby, either through the placenta during pregnancy or via breast milk post-birth. This type of immunity provides immediate, short-term protection to the newborn as their immune system develops .

HIV can be transmitted through unprotected sexual intercourse, sharing of needles, and transfusion of contaminated blood. Vertical transmission from mother to child can occur during childbirth or breastfeeding. Laboratory diagnosis includes tests such as the Western blot, immunoassays, and real-time PCR to detect HIV antibodies and viral RNA .

Antimicrobial stewardship refers to coordinated interventions designed to improve and measure the appropriate use of antibiotics. Its importance lies in combating antibiotic resistance, optimizing patient outcomes, and reducing adverse effects, including Clostridioides difficile infections. Effective stewardship programs involve monitoring and guiding antibiotic usage patterns within healthcare facilities .

IgG antibodies are the most abundant, providing sustained immunity and are involved in secondary immune responses. They can cross the placenta to provide passive immunity to the fetus. IgM is the first antibody produced in response to an antigen and is primarily involved in primary immune responses, forming a pentamer structure, making it effective in agglutination and neutralization of pathogens .

An autoclave operates on the principle of using high-pressure saturated steam to sterilize equipment and other items. The process typically involves heating to 121°C for about 15-30 minutes depending on the volume and contents. It is used to sterilize surgical instruments, laboratory glassware, and other critical items to eliminate all forms of microbial life, including spores .

Joseph Lister is acknowledged as the father of antiseptic surgery. He introduced the use of carbolic acid (phenol) to sterilize surgical instruments and clean wounds, which significantly reduced post-operative infections. This laid the foundation for modern surgical techniques in reducing infection risks .

The Spaulding classification categorizes medical devices based on their risk of infection to the patient. It classifies them as critical, semi-critical, or non-critical, which dictates the level of sterilization or disinfection required. Ensuring adherence to the correct sterilization process according to this classification enhances patient safety by preventing healthcare-associated infections .

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