O₂ Lumitrode
6.1116.000
Sensor leaflet
8.0109.8015EN / 2020-03-31
Metrohm AG
Ionenstrasse
CH-9100 Herisau
Switzerland
Phone +41 71 353 85 85
Fax +41 71 353 89 01
info@[Link]
[Link]
O₂ Lumitrode
6.1116.000
Sensor leaflet
8.0109.8015EN /
2020-03-31
Technical Communication
Metrohm AG
CH-9100 Herisau
techcom@[Link]
This documentation is protected by copyright. All rights reserved.
This documentation has been prepared with great care. However, errors
can never be entirely ruled out. Please send comments regarding possible
errors to the address above.
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents
Table of contents
1 Overview 1
1.1 Product description .............................................................. 1
1.2 Overview ................................................................................ 1
2 Functional description 3
3 Transport and storage 5
3.1 Checking the delivery ........................................................... 5
3.2 Storing the packaging .......................................................... 5
3.3 Unpacking the sensor ........................................................... 5
3.4 Storing the sensor ................................................................ 6
4 Preparing the sensor 7
5 Operation and control 8
5.1 Calibrating the sensor .......................................................... 8
6 Maintenance 11
6.1 Replacing the O ₂ cap ......................................................... 11
6.2 Cleaning the sensor ............................................................ 12
7 Troubleshooting 13
8 Disposal 15
9 Technical specifications 16
9.1 Ambient conditions ............................................................ 16
9.2 Dimensions .......................................................................... 16
9.3 Housing ............................................................................... 16
9.4 Measurement specifications .............................................. 16
￭￭￭￭￭￭￭￭ III
O₂ Lumitrode
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Overview
1 Overview
1.1 Product description
The O₂ Lumitrode is an optical sensor for measuring dissolved oxygen, i.e.
the amount of dissolved oxygen molecules in an aqueous phase. The sen-
sor works on the principle of luminescence quenching.
The O₂ Lumitrode can be connected to the following measuring instru-
ments:
￭ 913 pH/DO Meter
￭ 914 pH/DO/Conductometer
1.2 Overview
Figure 1 O₂ Lumitrode – Scope of delivery
1 O₂ Lumitrode 2 Calibration vessel
￭￭￭￭￭￭￭￭ 1
Overview ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
O₂ Lumitrode
Figure 2 O₂ Lumitrode
1 Plug, mini-USB 2 Fixed cable, 1.2 m
3 Pressure compensation opening 4 Steel ring with temperature sensor
5 O₂ cap, replaceable 6 Sensor membrane
Calibration vessel
Figure 3 Calibration vessel
1 Screw cap 2 Clamping collar
3 Vessel with sponge
2 ￭￭￭￭￭￭￭￭
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Functional description
2 Functional description
The sensor works on the principle of luminescence quenching.
Luminescence
A luminophore is embedded in the sensor membrane in the O₂ Lumitrode.
Irradiated light puts the luminophore into an excited state. When reverting
to the ground state, the luminophore emits light in the NIR range.
The time period between excitation and reverting to the ground state
(luminescence lifetime) causes a phase shift between irradiated and emit-
ted light.
Luminescence
Emitted light
Irradiated light
No collision
Energy transferred
without emissions
Collision with O₂
Luminophore Luminophore
ground state excited state
Luminescence quenching
Luminescence quenching
If the aqueous phase contains dissolved oxygen, then oxygen molecules
will collide with the luminophore. The collisions cause a transfer of energy
from the luminophore to the oxygen molecules. The luminophore mole-
cules thus revert to their ground state prematurely without radiation. The
luminescence is quenched.
Influence on the phase shift
The luminescence lifetime of the luminophore molecules varies. The longer
the luminescence lifetime, the greater the probability of a collision. Lumi-
nophore molecules with a long luminescence lifetime are therefore more
frequently quenched. The average luminescence lifetime decreases.
Accordingly, the phase shift between irradiated and emitted light is
reduced.
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The more oxygen molecules collide with the luminophore, the shorter the
average luminescence lifetime and the smaller the phase shift. The meas-
ured phase shift enables the calculation of the oxygen content (Stern-
Volmer law).
4 ￭￭￭￭￭￭￭￭
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Transport and storage
3 Transport and storage
3.1 Checking the delivery
Immediately upon arrival of the merchandise, check the shipment to
ensure absence of damage.
3.2 Storing the packaging
The product is supplied in extremely protective special packaging. Keep
this packaging, as only this ensures safe transportation of the product.
3.3 Unpacking the sensor
1 Unpacking and inspecting the sensor
Carefully remove the sensor and calibration vessel from the packag-
ing.
2 Preparing the sensor
According to Chapter Preparing the sensor (see chapter 4, page
7).
NOTICE
Defective sensors must be sent back for warranty processing within 2
months (from the date of delivery).
￭￭￭￭￭￭￭￭ 5
Storing the sensor ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
3.4 Storing the sensor
Always store the sensor in the calibration vessel within the specified tem-
perature range.
NOTICE
Improper handling
Damage to or reduced service life of the O₂ cap.
￭ Avoid major mechanical strain on the sensor membrane.
￭ Do not touch the sensor membrane with your fingers, the sponge
of the calibration vessel, brushes or similar.
Storing the sensor
1 Mounting the calibration vessel on the sensor
￭ Remove the screw cap from the calibration vessel.
￭ Place the screw cap of the calibration vessel on the sensor.
￭ Screw the calibration vessel onto the sensor. The sensor mem-
brane must not touch the sponge.
2 Store the sensor within the specified temperature range. See page
16.
6 ￭￭￭￭￭￭￭￭
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Preparing the sensor
4 Preparing the sensor
The sensor must be prepared before first use and after a long period of
non-use.
1 Connecting the sensor
￭ Connect the plug of the sensor to the socket i of the measuring
instrument. Observe the correct orientation (markings colored
orange in the image).
￭ The measuring instrument detects the sensor after a few seconds.
The sensor data is available in the sensor menu.
2 Calibrating the sensor
Carry out 2-point calibration with 0% and 100% air saturation. See
Calibrating the sensor (see chapter 5.1, page 8).
NOTICE
Contaminated sensor
Impairment of the measuring behavior or falsification of the measur-
ing results.
￭ The sensor surface and the sensor membrane must always be
clean before measurement.
￭￭￭￭￭￭￭￭ 7
Calibrating the sensor ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
5 Operation and control
5.1 Calibrating the sensor
The sensor must be calibrated before first use and after a long period of
non-use with a 2-point calibration (0% and 100% air saturation).
For routine calibration, a 1-point calibration with 100% air saturation in
the calibration vessel is usually sufficient.
NOTICE
Improper handling
Damage to the O₂ cap.
￭ Do not touch the sensor membrane with your fingers, the sponge
of the calibration vessel, brushes or similar.
NOTICE
Contaminated sensor
Impairment of the measuring behavior or falsification of the measur-
ing results.
￭ The sensor surface and the sensor membrane must always be
clean before measurement.
2-point calibration
Prerequisite:
￭ The sensor is connected to the measuring instrument.
1 Set the 2-point calibration 0%, 100% on the 91x meter:
Menu ▶ Parameters DO ▶ Calibration param. ▶ Calibration
points ▶ 0%, 100%
2 Calibration at 100% air saturation
￭ Detach the calibration vessel from the screw cap. Remove the cali-
bration vessel and screw cap from the sensor.
￭ Remove the screw cap from the calibration vessel.
8 ￭￭￭￭￭￭￭￭
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Operation and control
￭ Put some water on the sponge of the calibration vessel.
￭ Wait briefly so that the sponge can soak up the water.
￭ Pour off excess water.
￭ Place the screw cap of the calibration vessel on the sensor.
￭ Screw the calibration vessel onto the sensor. The sensor mem-
brane must not touch the sponge.
￭ Wait at least 30 seconds to allow the air space to saturate with
water vapor.
￭ Start calibration on the measuring instrument: press the CAL key.
￭ Follow the instructions of the instrument.
If necessary, set the Temp. compens. and Air press. comp..
3 Calibration at 0% oxygen
(with 6.2329.000 oxygen standard 0%)
￭ Remove the calibration vessel from the sensor.
￭ Tear open the sachet with oxygen standard 0% calibration solu-
tion.
￭ Immerse the sensor in the solution until it is over the metal ring.
￭ Swing the sensor briefly back and forth to remove adhering air
bubbles.
￭ Start calibration on the measuring instrument: press the CAL key.
￭ Once the calibration has been completed, the instrument displays
the results. If the measured values are within the defined limits,
the instrument automatically adopts the calibration parameters.
Otherwise the calibration is completed as a 1-point calibration.
After a few seconds the main dialog appears.
￭ Rinse off the sensor with water.
The instrument is ready for measuring.
1-point calibration
Prerequisite:
￭ The sensor is connected to the measuring instrument.
1 Set the 1-point calibration 100% on the 91x meter:
Menu ▶ Parameters DO ▶ Calibration param. ▶ Calibration
points ▶ 100%
2 Calibration at 100% air saturation
￭ Detach the calibration vessel from the screw cap. Remove the cali-
bration vessel and screw cap from the sensor.
￭ Remove the screw cap from the calibration vessel.
￭ Put some water on the sponge of the calibration vessel.
￭ Wait briefly so that the sponge can soak up the water.
￭￭￭￭￭￭￭￭ 9
Calibrating the sensor ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
￭ Pour off excess water.
￭ Place the screw cap of the calibration vessel on the sensor.
￭ Screw the calibration vessel onto the sensor. The sensor mem-
brane must not touch the sponge.
￭ Wait at least 30 seconds to allow the air space to saturate with
water vapor.
￭ Start calibration on the measuring instrument: press the CAL key.
￭ Follow the instructions of the instrument.
If necessary, set the Temp. compens. and Air press. comp..
Once the calibration has been completed, the instrument displays
the results and adopts them automatically.
After a few seconds the main dialog appears.
The instrument is ready for measuring.
10 ￭￭￭￭￭￭￭￭
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Maintenance
6 Maintenance
6.1 Replacing the O₂ cap
The measuring instrument displays the signal intensity with a status traffic
light:
Green The signal intensity is in the correct range.
Orange The signal intensity is in the range of the lower
limit value. A replacement of the O₂ cap will
soon be necessary.
Red The signal intensity is below the lower limit value.
Correct measuring can no longer be ensured.
Replace the O₂ cap. Replacement set:
6.5623.000
Replacing the O₂ cap
1 Unscrew existing O₂ cap.
2 Make sure that the sensor glass surface is clean and dry (see image).
If necessary, rinse the sensor glass surface with deionized water and
dry with a lint-free and non-scratching cloth.
3 If necessary, enter the serial number of the new O₂ cap on the 91x
meter: Menu ▶ Sensors ▶ Sensor list ▶ Serial no. cap
4 Screw on new O₂ cap.
￭￭￭￭￭￭￭￭ 11
Cleaning the sensor ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
6.2 Cleaning the sensor
NOTICE
Improper cleaning
Damage to the sensor and the O₂ cap.
￭ Do not bring the sensor and sensor membrane into contact with
any organic solvents.
￭ Never immerse the sensor in an ultrasonic bath.
NOTICE
Improper cleaning
Damage to or reduced service life of the O₂ cap.
￭ Avoid major mechanical strain on the sensor membrane.
￭ Do not touch the sensor membrane with your fingers, the sponge
of the calibration vessel, brushes or similar.
￭ Do not clean the sensor membrane with abrasive cleaner.
￭ Do not rub the sensor membrane dry after rinsing.
Heavy contamination can have a negative effect on the measuring behav-
ior or even falsify the measuring result. Always keep the sensor surface
and sensor membrane clean.
Cleaning after each measurement
1 Rinse off the sensor with water after each measurement.
Periodic cleaning
1 Regularly check the sensor and the sensor membrane visually for
contamination. Clean externally if necessary. Use water or a tenside
solution as cleaning agent.
2 If the pressure compensation opening is contaminated, remove
the contamination from the opening. Use water or a tenside solution
as cleaning agent.
12 ￭￭￭￭￭￭￭￭
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Troubleshooting
7 Troubleshooting
Measurement and calibration
Problem Cause Remedy
Measured value is DO calibration is faulty. ￭ Check/repeat calibration.
clearly wrong. ￭ Check the value for temperature compen-
sation.
￭ Check the value for atmospheric pressure
compensation.
Sensor membrane is conta- Clean the sensor membrane with water or ten-
minated. side solution.
Sensor membrane is Replace the O₂ cap.
leached out (photo-bleach-
ing).
The sensor is defective. Replace the sensor.
No measuring sig- Wrong measuring channel Switch on the corresponding measuring chan-
nal. Display: –.––– switched on. nel on the measuring instrument.
The sensor is not connec- Connect the sensor.
ted.
The plug of the sensor is Check the connection to the measuring instru-
not properly plugged into ment. See Preparing the sensor (see chapter 4,
the measuring instrument. page 7).
O₂ cap is missing. Screw the O₂ cap onto the sensor.
Sensor membrane is Replace the O₂ cap.
leached out (photo-bleach-
ing).
O₂ cap is defective. Replace the O₂ cap.
The sensor is defective. Replace the sensor.
The measuring input Send the measuring instrument to Metrohm
and/or the measuring Service for checks and, if necessary, repair.
channel is defective.
Signal intensity Sensor membrane is conta- Clean the sensor membrane with water or ten-
reduced – the sensor minated. side solution.
status display on the
Sensor membrane is Replace the O₂ cap.
measuring instru-
leached out (photo-bleach-
ment is in the yellow
ing).
or red range.
￭￭￭￭￭￭￭￭ 13
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
Problem Cause Remedy
The measured value O₂ cap is worn out. Replace the O₂ cap.
drift criterion is not
fulfilled.
Calibration: Error messages
Problem Cause Remedy
012-346 Signal too The measuring signal is ￭ Reduce the LED intensity (%) setting on
high above the limit value. the 91x meter: Menu ▶ Sensors ▶ Sen-
sor list ▶ LED intensity (%)
012-347 Signal too The measuring signal is ￭ Check the correct placement of the O₂ cap.
low below the limit value. If necessary, replace the O₂ cap.
012-348 Signal not The measuring signal is not ￭ For calibration at 100%: put some water
stable stable. on the sponge of the calibration vessel.
Repeat the calibration.
￭ For calibration at 0%: check or replace oxy-
gen standard 0%. Repeat the calibration.
￭ Check defined dphi limits in the sensor
menu: Menu ▶ Sensors ▶ Sensor list
Recommended limit values:
Limit value dphi 100%: Lower limit:
15.00 °, upper limit: 30.00 °
Limit value dphi 0%: Lower limit: 40.00 °,
upper limit: 60.00 °
912-106 Calibration The calibration data is out- ￭ Check or replace oxygen standard 0%.
limit value side of the specified limit Repeat the calibration.
values. ￭ Check defined dphi limits in the sensor
menu: Menu ▶ Sensors ▶ Sensor list
Recommended limit values:
Limit value dphi 100%: Lower limit:
15.00 °, upper limit: 30.00 °
Limit value dphi 0%: Lower limit: 40.00 °,
upper limit: 60.00 °
Refer to the measuring instrument manual for further troubleshooting
notes.
14 ￭￭￭￭￭￭￭￭
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Disposal
8 Disposal
This product is covered by European Directive, WEEE – Waste Electrical
and Electronic Equipment.
The correct disposal of your old product will help to prevent negative
effects on the environment and public health.
1 Disposing of the sensor
Put the sensor in electronic waste recycling.
More details about the disposal of your old product can be obtained from
your local authorities, from waste disposal companies or from your local
dealer.
￭￭￭￭￭￭￭￭ 15
Ambient conditions ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
9 Technical specifications
9.1 Ambient conditions
Nominal function range 0 °C - +60 °C observe functional
range of measuring
instrument
Storage and transport +5 °C - +45 °C at max. 80% relative
humidity, non-condens-
ing
9.2 Dimensions
Measurements
Shaft diameter 12 mm
Maximum installation length 140 mm
9.3 Housing
Materials
Shaft material ASA acrylonitrile styrene
acrylate copolymer
stainless steel
9.4 Measurement specifications
Measuring range 0.0 - +500% air saturation
0.00 - +50.00 mg/L
Resolution 0.1%
0.01 mg/L
16 ￭￭￭￭￭￭￭￭
￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Technical specifications
Measuring accuracy
Range 0 - 8 mg/L ±0.1 mg/L
Range 8 - 20 mg/L ±0.15 mg/L
Range 20 - 50 mg/L ±10%
Temperature range 0 - 60 °C Temperature sensor:
Pt100
Minimum immersion depth 35 mm
￭￭￭￭￭￭￭￭ 17