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Biosensors and Bioinstrumentation Exam

This document is the final examination for the course "Introduction to Biosensors and Bioinstrumentation". It contains questions in 4 sections worth a total of 45 marks. The exam will take place on December 18, 2018 from 4:30PM to 7:30PM in Room 2465. Students are expected to pledge honor and sign the document. The questions cover topics like random biological signals, blood pressure measurement techniques, capnography, pulse oximetry, biopotentials, electrocardiography, ultrasound Doppler flowmetry and their underlying principles and applications. Diagrams, waveforms and calculations may be required to fully answer the questions. An additional 5 marks are available as a bonus.

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Han ho
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0% found this document useful (0 votes)
28 views5 pages

Biosensors and Bioinstrumentation Exam

This document is the final examination for the course "Introduction to Biosensors and Bioinstrumentation". It contains questions in 4 sections worth a total of 45 marks. The exam will take place on December 18, 2018 from 4:30PM to 7:30PM in Room 2465. Students are expected to pledge honor and sign the document. The questions cover topics like random biological signals, blood pressure measurement techniques, capnography, pulse oximetry, biopotentials, electrocardiography, ultrasound Doppler flowmetry and their underlying principles and applications. Diagrams, waveforms and calculations may be required to fully answer the questions. An additional 5 marks are available as a bonus.

Uploaded by

Han ho
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Introduction to Biosensors and Bioinstrumentation

(ELEC 4810)

FINAL EXAMINATION

DATE: December 18, 2018


TIME: 4:30PM - 7:30PM
Venue: Rm 2465

PLEDGE OF HONOR
On my honor as a student of Hong Kong University of Science and
Technology, I have neither received aid from others nor given aid to
others while taking this exam. All of the answers represent my own work.

Signature: __________________ Date: ________________

Student Name:
Student Number:

This study source was downloaded by 100000866331519 from [Link] on 12-02-2023 05:45:28 GMT -06:00

[Link]
TOTAL: 45 marks + 5 marks BONUS.

1. (15 pts.) Sort-answering questions


a. Random signals, also called non deterministic signals, are those signals that
take random values at any given time. (i) Explain why biological signals are
random signals. (ii) The figures shown below are two random signals (blue
and red curves). What are the differences between two signals? (Identity at
least three major differences and explain why.)

b. (i) Describe the mechanisms of blood pressure measurement based on


Korotkoff's and oscillometric methods in Lab 3. (ii) Draw a schematic diagram
of automated blood pressure measurement system. (iii) Discuss the principle
and design of integrated pressure sensor used in the oscillometric blood pressure
measurement.
c. Capnography is the monitoring of the concentration or partial pressure of carbon
dioxide (CO2) in the respiratory gases. Its main development has been as a
monitoring tool for use during anesthesia and intensive care. (i) Explain the
mechanism of a capnometer. (ii) Draw a schematic diagram of the device. (iii)
Explain the waveform of capnogram as follow:

d. (i) What is oxygen saturation? (ii) Explain the mechanism of pulse oximetry
for the measurement of oxygen saturation. (iii) Why does pulse oximetry
require measurements at two wavelength? (iv) Why is the NIR source a LED
at 934 nm instead of at isosbestic point like a specific wavelength of 804 nm?

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[Link]
2. (5 pts.) “Brain waves”
a. The figure below shows typical neurons in cerebral cortex. Which types of
neurons will potentially contribute to EEG signal and which will not? Why?

b. Compressed spectral array (CSA) is a method for display of EEG signals for
long-term EEG monitoring of brain activity. Explain CSA method.
c. Meditation is a practice where an individual uses a technique - such as
mindfulness, or focusing their mind on a particular object, thought or activity -
to train attention and awareness, and achieve a mentally clear and emotionally
calm state. What do you learn from the figure below showing a CSA display
of EEG measurements from a human subject before and during meditation?
The horizontal axis is the signal frequency and vertical axis is the time period
of EEG measurement.

This study source was downloaded by 100000866331519 from [Link] on 12-02-2023 05:45:28 GMT -06:00

[Link]
3. (15 pts.) Biopotential and ECG
a. Describe the generation of action potential by a cell and explain the
regenerative nature of action potential.
b. Why do action potentials occur in nerve and muscle cells? Why is the
waveform of action potential in nerve cell very different from muscle cell?
Sketch typical action potentials of nerve cell and muscle cell, respectively.
c. Explain why a propagation action potential can be modeled as a traveling
electric dipole.
d. The “heart circuit” is shown in the figure below. Sketch the waveforms of
action potentials from SA node, AV node and ventricle, and explain why they
are different.

e. Einthoven defined first three bipolar limb leads (Lead I,II and III). Draw a
typical lead II electrocardiogram and label all waves (P, QRS, T) and intervals.
Explain what is happening electrically within the heart during each wave and
interval.
f. In addition to three bipolar limb leads, there are three augmented unipolar
limb leads (aVR, aVL and aVF). Describe how three augmented leads are
defined and measured.
g. Figure below shows ECGs from simultaneous aVR, aVL and aVF leads.
Sketch the cardiac dipole vector loop (direction and amplitude) for P-wave,
QRS complex and T-wave in the frontal plane (a plane defined by Einthoven’s
triangle).

h. Draw the circuit used in Lab-1 for ECG measurement. Describe how the
bandpass filter works in the circuit.

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i. What is common mode noise in a differential amplifier? Explain how a driven
right leg circuit reduce the common mode noise.
4. (15 pts.) Ultrasound Doppler Flowmetry
a. Explain the piezoelectric effect and how to generate and detect ultrasound
wave by using piezoelectric material(s).
b. Describe the mechanism of ultrasound Doppler flowmetry for the
measurement of blood flow. Discuss the limitations of ultrasound Doppler
flowmetry.
c. The ultrasound transducer and receiver are normally integrated in a handheld
device for convenience of use. If the geometry of blood flow measurement is
shown in the figure below, derive the relationship between Doppler shift and
blood velocity.

d. Discuss the technique of directional ultrasound Doppler flowmetry with


detailed mathematical descriptions. Design a detection system to automatically
detect the blood flow direction (show schematic diagram of the system).
e. You have conducted ultrasound Doppler flowmetry measurement in Lab 5.
Give a brief description of the measurement and explain the purpose of each
step.

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Common questions

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Ultrasound Doppler flowmetry limitations include angle dependency of measurements, which affects the accuracy of velocity calculations, and the inability to measure slow or turbulent flows accurately. These limitations are significant, as they can lead to errors in clinical assessments of blood flow, particularly in complex vascular structures or in conditions with abnormal flow patterns .

Capnometers measure CO2 concentration by continuously sampling respiratory gases and analyzing the CO2 levels using non-dispersive infrared sensors, which detect the specific wavelengths absorbed by CO2 molecules. This method provides real-time data on ventilation status, helping monitor anesthesia and intensive care patients .

Random biological signals are crucial because they reflect the complex, fluctuating processes within biological systems, such as heart rates or neural activity, which are non-deterministic in nature. These signals exhibit unpredictability and variability, necessitating advanced analysis techniques for accurate interpretation and decision-making in bioinstrumentation .

The action potential in nerve cells differs from muscle cells primarily in duration and amplitude. Nerve cells exhibit a sharp, rapid spike due to quick ion exchange through voltage-gated channels, essential for rapid signal transmission. Muscle cells show a prolonged plateau phase, resulting from sustained calcium influx, crucial for muscle contraction. These waveform differences reflect their roles—rapid communication in nerves and prolonged contraction in muscles .

Automated blood pressure systems significantly enhance healthcare by providing accurate and consistent measurements with minimal user intervention, increasing efficiency in clinical settings. These systems afford convenience and reliability, crucial for large-scale health assessments and continuous monitoring, thus improving patient care and outcomes .

Piezoelectric materials in Doppler flowmetry transducers convert electrical signals into mechanical vibrations when a voltage is applied, producing ultrasound waves. Upon encountering body tissues, these waves are reflected back and detected by the same material, which converts them back into electrical signals. This capability allows for precise measurement of blood flow based on the Doppler effect, where the frequency shift of the reflected waves corresponds to flow velocity .

Capnography enhances patient safety by providing a real-time assessment of ventilatory status, helping to quickly detect respiratory disturbances such as hypoventilation, apnea, or airway obstructions. Early detection allows for timely intervention, reducing the risk of complications during anesthesia and in intensive care settings .

A driven-right-leg circuit minimizes common-mode noise by injecting a voltage equal in magnitude but opposite in phase to the common-mode signal into the subject's body. This reduces the noise interference by canceling it out, enhancing the clarity of ECG recordings. It is critical in bioinstrumentation to ensure the accuracy of the bioelectrical signals being measured .

CSA method enhances long-term EEG monitoring by graphically compressing the frequency content of EEG signals over time, allowing easier visualization of changes in brain activity patterns. This aids in identifying trends or anomalies over extended periods, thus improving diagnostic accuracy in neurological conditions .

Pulse oximetry uses two wavelengths to distinguish between oxyhemoglobin and deoxyhemoglobin due to their different light absorption spectra. By comparing the absorption ratios at two wavelengths (usually in the red and infrared regions), it effectively calculates oxygen saturation, providing a non-invasive and reliable method to monitor patients' oxygenation status .

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