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Specimen Collection and Transport Guide

This document provides guidelines for specimen collection, handling, and transport for microbiological testing. It discusses selecting the appropriate specimen type and collection method based on the type of infection or site being tested. It also covers proper labeling, storage, and transport conditions to maintain specimen integrity and viability. Priority levels for different specimen types are defined based on their suitability for growing certain pathogens. Specific collection instructions are given for various specimen sources like blood, body fluids, bones, skin/wounds, and respiratory, genital, and urinary specimens.

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Mari Jo Parillo
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90 views2 pages

Specimen Collection and Transport Guide

This document provides guidelines for specimen collection, handling, and transport for microbiological testing. It discusses selecting the appropriate specimen type and collection method based on the type of infection or site being tested. It also covers proper labeling, storage, and transport conditions to maintain specimen integrity and viability. Priority levels for different specimen types are defined based on their suitability for growing certain pathogens. Specific collection instructions are given for various specimen sources like blood, body fluids, bones, skin/wounds, and respiratory, genital, and urinary specimens.

Uploaded by

Mari Jo Parillo
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Parillo, Mari Jo - BMLS

Specimen Collection, Handling and Transport maintain the viability of N.


gonorrhoeae for extended periods.
 Specimen should be collected during the  0.025% Sodium Polyanethol
acute phase of illness. Sulfonate-Blood (Neisseria)
 Select the correct anatomic site.
 Cleanse site before collection Level of Specimen Prioritization
 Specimen should be a representation of  Level 1: Amniotic fluid, Blood, Brain,
infected site. CSF, Heart valves and Pericardial Fluid
 Quantity should be sufficient.  Level 2: Bones, feces, tissue, body
 Specimen should be collected before fluids
antibiotics.  Level 3: Catheter tip, urine, and tissue
 Swabs are poor specimen.  Level 4: Urine, tissue swabs in holding
 For lesions, wounds and abscesses, media.
collect from advancing margins.
 Give clear instructions to specimen. Specimen Storage
 Label specimen accurately
 Refrigerator: urine, stool, viral
Specimen Containers specimen, sputa and swabs.
 Incubator: CSF
 Use sterile containers.  Ambient Temperature: Fungal specimen
 Swabs for specimen from URT, external  Freezer temperature: serum for
ear, eye and genital tract serological studies
 Swabs are not recommended.  (-20 for 1 week), Tissues (-70 for long
 Use Dacron or calcium alginate storage)
swabs.
 Two swabs for gram stain and culture
Specimen Labelling

 Patient information (Complete name,


Date and time of collection, Test to be
performed)
 Identification of mislabelled specimen
or requisition form should not be done
over the form.
 Use exact anatomic site. Blood Culture

Transport  70% alcohol and iodophore


 Draw during febrile episodes
 Transport should be within 30 minutes.  2 sets from left and right arm
 Containers should be leak proof.  Use SPS tube (Sodium polyanethole
 Use proper preservative. sulfonate.
 Boric acid at room temperature for
24 hours. Body Fluids
 Charcoal- absorbs fat.
 Amniotic fluid
 Stuart, Amies, Cary Blair, transglow,
 Abdominal fluid
JEMBEC- provide a selective
 Peritonial fluid
environment that will protect and
 Joint
Parillo, Mari Jo - BMLS

 Synovial  Skin: scrape skin at the leading edge of


 Pericardial lesion.
 Pleural
Lower Respiratory Tract
Bone
 Anaerobic culture-catheter
 Disinfect skin before surgery.
Upper Respiratory Tract
 Sample should come from affected area.
 Requires homogenization  Nasopharynx
 Pharynx
CSF
Sputum
 Disinfect skin
 Consider rapid testing  First morning
 Storage: 6 hours ate 37 degree Celsius  Rinse mouth with sterile water prior
 Neisseria meningitiditis collection
 Haemophillus influenzae  TB-3 concecutive early morning
specimen
Conjunctiva  Deep cough
 Both eyes Types of Sputum
 Aerobic swab-stuarts or Amies or sterile
saline  Bloody
 Rusty
Corneal Scraping
 Purulent
 Instill local anesthetic before collection  Foamy white
 Frothy pink
Intrauterine Device (IUD)
Sputum Criteria
 Culture to detect Actinomyces spp.
Catheter

 Maki roll technique of inoculation


Genital Tract

 Bartholin Cyst
 Cervix
 Cul-de-sac Urine
 Endometrium  Midstream clean catch/First morning
 Urethra urine.
 Vagina  Straight Catheter- let 15mL pass
 Prostate  Indwelling- aspirate 5-10mL
Nails and Skin  Supra pubic- needle aspiration

 Wipe with 70% alcohol


 Hair: collect with intact shaft
 Nails: clippings of affected area

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