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Enzyme Action and Inhibition Models

1. Enzymes are globular proteins that catalyze biochemical reactions by lowering the activation energy. They are produced by living cells and act as biological catalysts. 2. The key models of enzyme action are the lock and key model proposed by Emil Fischer in 1890 and the induced fit model proposed by Koshland in the 1950s based on new evidence. 3. Factors like pH, temperature, and inhibitors can affect the rate of enzyme activity. Enzymes function most effectively within a narrow pH range called the optimum pH.

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0% found this document useful (0 votes)
21 views4 pages

Enzyme Action and Inhibition Models

1. Enzymes are globular proteins that catalyze biochemical reactions by lowering the activation energy. They are produced by living cells and act as biological catalysts. 2. The key models of enzyme action are the lock and key model proposed by Emil Fischer in 1890 and the induced fit model proposed by Koshland in the 1950s based on new evidence. 3. Factors like pH, temperature, and inhibitors can affect the rate of enzyme activity. Enzymes function most effectively within a narrow pH range called the optimum pH.

Uploaded by

bszool006
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

[Link] Name ………………….

Chapter No 03 Section …………………

Subject: - Biology (Intermediate I) TIME ALLOWED:- 10 Mins.


Dated: ___________ (Objective) MAX. MARKS: - 10
[Link].1 Four possible options have been given. Tick () mark the correct option. (10)

1. An enzyme without its co-factor is termed as:


a) Coenzyme b) Holoenzyme
c) Apoenzyme d) Prosthetic group
2. Lock and Key Model regarding the substrate and enzyme interaction was proposed in 1890 by:
a) Emil Fischer b) Koshland
c) Robert Koch d) Louis Pasteur
3. An enzyme is a three dimensional ______ protein.
a) Fibrous b) Straight
c) Globular d) Branched
4. On the basis of new evidences Induce Fit model was proposed by:
a) Koshland b) Emil Fischer
c) Robert Koch d) Louis Pasteur
5. Reversible Inhibitors form weak linkages with the:
a) Enzyme b) Reactant
c) Product d) Substrate
6. The optimum pH of Catalase is:
a) 2.50 b) 4.60
c) 5.70 d) 7.60
7. Inhibitors occupy the active sites by forming covalent bonds or they may physically block the active
sites.
a) Reversible b) Irreparable
c) Reparable d) Irreversible
8. If the non-protein part is covalently bonded to enzyme, it is known as a:
a) Coenzyme
b) Co-factor
c) Holoenzyme
d) Prosthetic group
9. Enzymes are very _______ in their action.
a) General b) Specific
c) Precise d) Exact
10. If you add more substrate to already occurring enzymatic reaction and it has no effect on the rate of
reaction. What is the term given for this situation?
a) Saturation b) Denaturation
c) Composition d) Inhibition
[Link] Name ………………….

Chapter No 03 Section …………………

Subject: - Biology (Intermediate I) TIME ALLOWED:- 30 Mins.


Dated: ___________ (Subjective) MAX. MARKS: - 18

Q. 2 Attempt short questions: - (5x2=10)


I. What are enzymes and coenzymes?
II. Differentiate between irreversible and reversible inhibitors.
III. What is a co-factor?
IV. What is Induced Fit Model?
V. What is Apoenzyme?

Q. 3 Long questions: - (4x2=8)

1. a) What are enzymes? What is their chemical composition and location?

b) What do you know about the following?


i- Co-factors ii- Prosthetic group

2. a) Explain the characteristics of enzymes.


b) Explain the mechanism of enzyme action.
[Link] Name ………………….

Chapter No 03 Section …………………

Subject: - Biology (Intermediate I) TIME ALLOWED:- 10 Mins.


Dated: ___________ (Objective) MAX. MARKS: - 10
[Link].1 Four possible options have been given. Tick () mark the correct option. (10)

1. The enzymes are:


a) Fibrous proteins
b) Globular proteins
c) Angular proteins
d) Spherical proteins
2. At low concentration of substrate the reaction rate is directly proportional to the available:
a) Enzyme b) Reactant
c) Product d) Substrate
3. Poisons, like cyanide, antibodies, anti metabolites and some drugs are examples of:
a) Enzymes b) Coenzymes
c) Inhibitors d) Holoenzymes
4. The activation energy of the reactions is lowered by:
a) Coenzymes b) Substrate
c) Enzymes d) Product
5. The detachable co-factor is known as an activator if it is:
a) Organic ion b) Non-ionic
c) Inorganic ion d) Non-polar
6. Every enzyme functions most effectively over a narrow range of pH known as the:
a) Best pH b) Minimum pH
c) Maximum pH d) Optimum pH
7. Any enzyme reacts only with its specific:
a) Surface b) Product
c) Substrate d) Reactant
8. The reversible inhibitors usually constitute:
a) Strong linkage with enzyme
b) Weak linkage with enzyme
c) No linkage with enzyme
d) Medium linkage with enzyme
9. The catalytic activity of an enzyme is restricted to its portion called:
a) Active site b) Passive site
c) Intermediate site d) Inhibiting site
10. 5.5 is the optimum pH value for the enzyme:
a) Arginase b) Sucrase
c) Enterokinase d) Amylase
[Link] Name ………………….

Chapter No 03 Section …………………

Subject: - Biology (Intermediate I) TIME ALLOWED:- 30 Mins.


Dated: ___________ (Subjective) MAX. MARKS: - 18
Q. 2 Attempt short questions: - (5x2=10)
I. Non-competitive inhibitors.
II. Define optimum pH of enzyme.
III. Differentiate between prosthetic group and coenzyme.
IV. What are irreversible inhibitors?
V. How does an Enzyme accelerate a metabolic reaction?

Q. 3 Long questions: - (4x2=8)

1. a) Discuss the different factors which affect the rate of enzyme action.
b) Differentiate between irreversible and reversible inhibitors.

2. a) How does low or high temperature affect enzyme activities?


b) How does an Enzyme accelerate a metabolic reaction?

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