Scribd
Upload
36 views4 pages

Microscope Use and Slide Examination Guide

The document provides instructions for a laboratory exercise on using a compound light microscope. It includes three parts: 1) labeling microscope parts and identifying functions, 2) using the microscope to view slides at different magnifications, and 3) answering analysis questions. Students are asked to view sample slides at 4x, 10x, 40x, and 100x magnifications, using coarse and fine focus as needed. They must also identify when to use high versus low power objectives and explain the purpose of adjusting the diaphragm.

Uploaded by

Lili13
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
36 views4 pages

Microscope Use and Slide Examination Guide

The document provides instructions for a laboratory exercise on using a compound light microscope. It includes three parts: 1) labeling microscope parts and identifying functions, 2) using the microscope to view slides at different magnifications, and 3) answering analysis questions. Students are asked to view sample slides at 4x, 10x, 40x, and 100x magnifications, using coarse and fine focus as needed. They must also identify when to use high versus low power objectives and explain the purpose of adjusting the diaphragm.

Uploaded by

Lili13
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

NAME:

Laboratory Exercise 1

The Microscope

Part 1. Microscope Labelling and Identification of functions

1. Go to [Link]
2. Click on the Guide link (bottom of the home page).
3. Click through the six parts of the Guide, starting with the Introduction. You can use the arrows at the
bottom of the Guide box to guide you through the chapters.
4. When you have completed all six sections, click Close.
5. Next click on the Learn link (bottom of the page), which will take you to an image of a microscope with
question marks.
6. Starting at the top of the microscope, click on the question marks identifying the parts of the microscope.
7. Read the description of the part of the microscope and take notes as needed.
8. Continue clicking on question marks until all turn to green check marks.
a) Do not forget to click on the question marks for items associated with the microscope.
b) You may click on any green check mark to review any part of the microscope.
9. Click on the Next button (bottom right).
10. Start on the left and click on the question mark. When the lens enlarges, click on each question mark until
each turn into a green check mark. Read the descriptions and take notes as needed.
11. Click on the Next button (bottom right).
12. Click on the Dry Slide and Oiled Slide buttons to see the difference in why immersion oil is used for
the 100X objective lens.
13. Click on the Next button (bottom right).
14. Click on the Eyepiece Options and Lens Options to learn about calculating total magnification. Try all
combinations and see how the Letter E slide image changes.
15. Click on the Next button (bottom right) to return to the home page.
16. Label the parts of the microscope on the next page.
The compound light microscope

1.

2.

3.
8.
4.

9.

5.
10.
6.
11.
7.

12.

Answer:

1. Eyepiece
2. Eyepiece Tube
3. Nosepiece
4. Objective Lens
5. Stage Clips
6. Diaphragm
7. Light Source
8. Arm
9. Stage
10. Coarse Focus
11. Fine Focus
12. Base
Part 2. How to use a compound microscope to view slides.

1. Click on the Explore link (bottom of the home page).


2. Click on the question mark on the slide box.
3. In the Slide Catalog, click on the Sample Slides.
4. Click on the Letter E slide. It will automatically be placed on the stage of the microscope.
5. When the Microscope View window opens, make sure that the 4X circle is highlighted in blue.
6. NOTE: Always begin examining slides with the lowest power objective.
7. Use the slider under Coarse Focus to find the E.
8.
9. NOTE: The coarse adjustment knob should only be used when you are viewing a specimen with the 4X
objective lens.
10. Then use the slider under Fine Focus to make the image “crisp and clear.”
11. You can click on the E in the viewing window to move the image and visualize different parts.
12. Next click on the 10X circle. The nosepiece on the microscope will rotate automatically.
13. Repeat steps 6 – 8 to see part of the E. Sketch your view of the letter E at 10X in the results area.
14. Click on the 40X circle and repeat steps 7 & 8. You may need to use the slider under Light Adjustment for
better visualization. Sketch your view of the letter E at 40X in the results area. Click on the 100X circle. A
notice to add immersion oil will open.
15. Click on the question mark on the immersion oil bottles to add oil to the microscope.
16. Repeat steps 7 & 8. You may need to use the slider under Light Adjustment again for better visualization.
17. When you have visualized the Letter E slide using all 4 objective lenses, click on Remove Slide (top right).
18. Read the notice about using lens paper to clean the immersion oil off the microscope and click on the
question mark over the lens paper. Choose wisely!
19. Follow the same procedure for the onion root, spider leg, and bacterial (Gram stain mix) slides. Draw the
images using the total magnification as indicated. For your drawing, you can draw in a piece of paper, take
a picture of your drawing, and paste them in a separate document. Make sure to write the appropriate label
for identification (specimen name and magnification). You can use other forms/media to draw. Send a
copy of your drawings in a PDF file (Activity 1_Surname).

Onion root (10x)


Spider leg (100x)
bacterial (Gram stain mix) (100x)

Part 3. Analysis and Review

● Use the information from “Virtual Microscope” lab activities to answer the questions below.
● Answer using complete sentences.
● Use “quotation marks” to cite specific evidence from the information you collected.

1. What objective lens you should have in place to begin looking at your specimen? Explain why.

Ans: 4x lens will be used since it has a least magnification but a larger field of view which allows specimen
to be seen clearly.

2. Explain why this statement is right or wrong. (Yes, make a decision and defend your choice.) “You only
use the coarse focus knob when you have the 4X scanning objective in place.”

Ans: No, because the fine adjustment knob can only be used with 4X scanning to focus on a specimen
more closely.
3. When should the high-power objective be used? How about the low power objective?
Ans. We should use high power objective when we are observing fine details within a specimen
sample. And we are using low power objective when we examining a large specimen or looking thoroughly
into a smaller specimen.

4. Under what conditions would you adjust the diaphragm?


Ans. Diaphragm has different sized holes and is used to vary intensity and size of the cone of light that
is projected upward into the slide. There is no set rule regarding which setting to use for a particular power.

Common questions

Powered by AI

To view a specimen slide using a virtual microscope: place the slide on the stage, start with the 4X objective lens for a broad view, center, and focus using the coarse and fine focus knobs, rotate to the 10X lens and refocus, increase to the 40X if needed, adjusting light and focus, and finally apply immersion oil to use the 100X lens, maintaining focus with fine adjustments throughout.

The diaphragm should be adjusted based on the specimen and magnification level to control the intensity and size of the light cone reaching the slide. Different apertures allow varying degrees of light, affecting contrast and resolution. Adjusting the diaphragm is essential under different lighting conditions and when switching between low and high-power objectives.

Beginning with the lowest power objective, such as the 4X lens, is recommended because it provides the largest field of view, allowing easier location and centering of the specimen. This facilitates observing the overall structure before switching to higher magnification for detailed examination.

Digital tools, such as virtual microscope simulations, can be incorporated into lab exercises to provide interactive, risk-free learning experiences. These tools offer scalability, accessibility for remote learning, capacity for repeated use, and immediate feedback, enhancing understanding of microscope operation and slide examination, and effectively preparing students for actual lab environments.

A high-power objective is necessary when observing fine details within a specimen, such as cellular structures or intricate patterns not visible at lower magnifications. Conversely, a low-power objective is optimal for examining larger specimens or for obtaining an overview of the entire sample, allowing for more general interactions to be observed initially before delving into details.

Immersion oil is used with the 100X objective lens to minimize light refraction, allowing clearer and sharper images of specimens. The oil has a similar refractive index to glass, ensuring that light passes through the specimen correctly and is not lost or bent as much as it would be through air, thus enhancing visualization and resolution at high magnifications.

The primary components of a compound microscope include the eyepiece, eyepiece tube, nosepiece, objective lenses, stage clips, diaphragm, light source, arm, stage, coarse focus, fine focus, and base. The eyepiece is used for viewing, the tube holds the eyepiece, the nosepiece rotates the objective lenses, which magnify the specimen. Stage clips hold the slide, the diaphragm controls light intensity, the light source illuminates the specimen, the arm supports the optical elements, the stage is where the specimen is placed, and the coarse and fine focus knobs are used for adjusting clarity. The base provides stability.

To transition between different objective lenses while maintaining focus, start with the specimen centered using a low-power lens (4X). Adjust focus using the coarse and then the fine knob. Move to the next higher objective (10X) by rotating the nosepiece, using only fine adjustments to refine focus. Before switching to 40X or 100X, ensure the specimen remains centered, and adjust lighting as needed to maintain clarity and focus throughout the transition.

Cleaning the microscope after using immersion oil is crucial to prevent oil residues from damaging the objective lenses and affecting future observations. The recommended steps include removing the slide, using lens paper to carefully clean any oil off the lens surfaces, ensuring no oil leaks into the mechanics of the lens or microscope, and storing the microscope in a dry, clean environment.

The coarse focus knob is primarily used when initially observing a specimen with the 4X objective lens because it quickly brings the specimen into general focus. The fine focus knob is used to increase the resolution of the specimen under all lenses, especially at higher magnifications like 10X, 40X, or 100X, to achieve a sharp image.

You might also like