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chapter 3
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Quiz Type Graded Quiz

Points 65
Assignment Group Assignments
Shuffle Answers No
Time Limit No Time Limit
Multiple Attempts No
View Responses Always
Show Correct Answers Immediately
One Question at a Time No

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Score for this quiz: 0 out of 65 *

Submitted Oct 17 at 7:28am

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Unanswered
Question 1 0 / 1 pts

Which of the following is not equal to 1 mm?

0.001 m

1,000,000 nm

0.1 cm

Correct Answer 100 μm

1,000.000.000 pm

Unanswered
Question 2 0 / 1 pts

What structure does light pass through after leaving the condenser in a compound light microscope?

Ocular lens

Objective lens

Correct Answer Specimen

 Illuminator

Unanswered
Question 3 0 / 1 pts

Which of the following pairs is mismatched?

Nigrosin — negative stain

Methylene blue — simple stain

Acidic dye — capsule stain

Correct Answer Basic dye — negative stain

Crystal violet — simple stain

Unanswered
Question 4 0 / 1 pts

Which of the following places the steps of the Gram stain in the correct order?
1-Alcohol-acetone
2-Crystal violet
3-Safranin
4-Iodine

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1-2-3-4

2-1-4-3

Correct Answer 2-4-1-3

4-3-2-1

1-3-2-4

Unanswered
Question 5 0 / 1 pts

Which of the following pairs is mismatched?

Alcohol-acetone — decolorizer

Crystal violet — basic dye

Correct Answer Safranin — acid dye

Iodine — mordant

Carbolfuchsin — basic dye

Unanswered
Question 6 0 / 1 pts

All of the following statements are true, except:

It is used to identify members of the genus Mycobacterium.

Acid-fast cells retain the primary dye after treatment with acid-alcohol.

Correct Answer If cells are acid-fast, they are gram-negative.

Acid-fast cells appear red in a completed acid-fast stain.

Non-acid-fast microbes appear blue in a completed acid-fast stain.

Unanswered
Question 7 0 / 1 pts

What is the purpose of Grams iodine as a mordant in the Gram stain?

Remove the simple stain

Make the bacterial cells larger

Make the flagella visible

Correct Answer
Prevent the crystal violet from leaving the cells

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Make gram-negative cells visible

Unanswered
Question 8 0 / 1 pts

Which of the following places the steps in the correct sequence?

1-Staining
2-Making a smear
3-Fixing

1-2-3

3-2-1

Correct Answer 2-3-1

1-3-2

The order is unimportant.

Unanswered
Question 9 0 / 1 pts

The negative stain is used to ________.

 visualize endospores

determine Gram reaction

determine flagella arrangement

visualize fimbriae

Correct Answer determine cell size

Unanswered
Question 10 0 / 1 pts

Simple staining is often necessary to improve contrast in which microscope?

Correct Answer Compound light microscope

Phase-contrast microscope

Darkfield microscope

Fluorescence microscope

Electron microscope

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Unanswered
Question 11 0 / 1 pts

A ________ can be used to view the internal structures of cells in its native state?

compound light microscope

Correct Answer phase-contrast microscope

scanning acoustic microscope

fluorescence microscope

electron microscope

Unanswered
Question 12 0 / 1 pts

Which microscope uses visible light?

Confocal microscope

Correct Answer Differential interference contrast microscope

Fluorescence microscope

Scanning acoustic microscope

 Scanning electron microscope

Unanswered
Question 13 0 / 1 pts

Which microscope achieves the highest magnification and greatest resolution?

Compound light microscope

Phase-contrast microscope

Darkfield microscope

Fluorescence microscope

Correct Answer Electron microscope

Unanswered
Question 14 0 / 1 pts

While using one of the following microscopes, the observer does not have to look directly at an image
through a lens.

Compound light microscope

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Phase-contrast microscope

Darkfield microscope

Fluorescence microscope

Correct Answer Electron microscope

Unanswered
Question 15 0 / 1 pts

________ produces an image of a light cell against a dark background without details of internal
structures.

Compound light microscope

Phase-contrast microscope

Correct Answer Darkfield microscope

Fluorescence microscope

Electron microscope
Unanswered
Question 16 0 / 1 pts

A virus measures 100 nm in length. What is its length in μm?

10 μm

1 μm

Correct Answer 0.1 μm

0.01 μm

0.001 μm

Unanswered
Question 17 0 / 1 pts

Which of the following microscopes is not useful for observing living cells?

Phase-contrast microscope

Darkfield microscope

Scanning acoustic microscope

Correct Answer Scanning electron microscope

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Brightfield microscope

Unanswered
Question 18 0 / 1 pts

A microorganism measures 5 μm in length. What would be its length in mm?

500 mm

50 mm

0.5 mm

0.05 mm

Correct Answer
0.005 mm

Unanswered
Question 19 0 / 1 pts

Which of the following correctly traces the path of light through the compound microscope?

Correct Answer Light source — condenser — specimen — objective lens — ocular lens
 Condenser — light source — specimen — ocular lens — objective lens

Light source — specimen — condenser — objective lens — ocular lens

Condenser — light source — specimen — objective lens — ocular lens

Light source — condenser — objective lens — specimen — ocular lens

Unanswered
Question 20 0 / 1 pts

Figure 3.1

In Figure 3.1, line "c" points to the microscope's ________.

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illuminator

Correct Answer
condenser

ocular lens

objective lens

Unanswered
Question 21 0 / 1 pts

Which of the following microscopes can be used to visualize DNA or botulinum toxin?

Compound light microscope

Phase-contrast microscope

Correct Answer Scanning tunneling microscope

Confocal microscope

Scanning electron microscope

Unanswered
Question 22 0 / 1 pts
 Which of the following microscopes can be used to observe a specimen that emits light when illuminated
with an ultraviolet light?

Compound light microscope

Phase-contrast microscope

Darkfield microscope

Correct Answer Fluorescence microscope

Electron microscope

Unanswered
Question 23 0 / 1 pts

Which of the following microscopes is the most useful for visualizing a biofilm?

Compound light microscope

Phase-contrast microscope

Fluorescence microscope

Correct Answer Scanning acoustic microscope

Transmission electron microscope

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Unanswered
Question 24 0 / 1 pts

Which microscope takes advantage of differences in the refractive indexes of cell structures?

Compound light microscope

Correct Answer Phase-contrast microscope

Darkfield microscope

Fluorescence microscope

Electron microscope

Unanswered
Question 25 0 / 1 pts

You are performing a Gram stain on gram-positive bacteria and you stop after the addition of the first dye.
What is the appearance of the bacteria at this point?

Correct Answer Purple

Red
 Colorless

Brown

Unanswered
Question 26 0 / 1 pts

If a Gram stain is performed correctly, what is the color of gram-negative bacteria, viewing by a compound
light microscope, after adding the iodine as a mordant?

Correct Answer Purple

Red

Colorless

Brown

Unanswered
Question 27 0 / 1 pts

If a Gram stain is performed correctly, what is the color of gram-negative bacteria, viewing by a compound
light microscope, after the decolorization?

Purple

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Red

Correct Answer Colorless

Brown

Unanswered
Question 28 0 / 1 pts

If a Gram stain is performed correctly, what is the color of gram-positive bacteria, viewing by a compound
light microscope, after adding the counterstain?

Correct Answer
Purple

Red

Colorless

Brown

Unanswered
Question 29 0 / 1 pts

Which type of staining technique is the most useful in helping clinicians to decide which antibiotic to
 prescribe for a bacterial infection?

Negative stain

Simple stain

Correct Answer Gram stain

Endospore stain

Flagella stain

Unanswered
Question 30 0 / 1 pts

Which of the following pairs is mismatched?

Confocal microscope — produces a three-dimensional image

Darkfield microscope — uses visible light

Correct Answer Fluorescence microscope — uses a fluorescent light

Scanning electron microscope — produces a three-dimensional image

Scanning tunneling microscope — allows visualization of atoms

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Unanswered
Question 31 0 / 1 pts

What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective lens?

4.5x

10x

45x

100x

Correct Answer
450x

Unanswered
Question 32 0 / 1 pts

You are studying a cell structure that is approximately 100 nm in size. Which of the following provides the
greatest magnification you can use to see this structure?

Brightfield microscope

Darkfield microscope
 Transmission electron microscope

Phase-contrast microscope

Correct Answer Scanning electron microscope

Unanswered
Question 33 0 / 1 pts

Which of the following microscope uses two beams of light to produce a three-dimensional color image?

Fluorescence microscope

Phase-contrast microscope

Darkfield microscope

Correct Answer DIC microscope

Electron microscope

Unanswered
Question 34 0 / 1 pts

Which of the following microscope can be used to see intracellular detail in a living cell?


Fluorescence microscope

Correct Answer Two-photon microscope

Atomic force microscope

Transmission electron microscope

Brightfield microscope

Unanswered
Question 35 0 / 1 pts

Which of the following microscope can be used to observe viruses and the internal structure of thinly
sectioned cells?

Correct Answer Transmission electron microscope

Darkfield microscope

Fluorescence microscope

Brightfield microscope

Scanning electron microscope

Unanswered
Question 36 0 / 1 pts

Which of the following microscopes can be used to see detail of a 300-nm virus?

Fluorescence microscope

Phase-contrast microscope

Darkfield microscope

DIC microscope

Correct Answer
Electron microscope

Unanswered
Question 37 0 / 1 pts

Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin.
Through the microscope, the green structures are ________.

cell walls

capsules

Correct Answer endospores

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flagella

The answer cannot be determined

Unanswered
Question 38 0 / 1 pts

Which step in the Gram stain is the critical step in differentiating gram-positive cells from gram-negative
cells?

Safranin

Correct Answer
Alcohol-acetone

Iodine

Crystal violet

Unanswered
Question 39 0 / 1 pts

You find colorless areas in cells in a Gram-stained smear. What should you do next?

An acid-fast stain
 A flagella stain

A capsule stain

Correct Answer An endospore stain

A simple stain

Unanswered
Question 40 0 / 1 pts

Which of the following microscopes can be used for observing the surfaces of the cells and viruses?

Phase-contrast microscope

Darkfield microscope

Fluorescence microscope

Brightfield microscope

Correct Answer Scanning electron microscope

Unanswered
Question 41 0 / 1 pts

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Bacterial smears are fixed before staining to ________.

Correct Answer affix the cells to the slide

make their walls permeable

accept stain

make the cells visible

Unanswered
Question 42 0 / 1 pts

The resolution of a microscope can be improved by changing the ________.

condenser

fine adjustment knob

Correct Answer wavelength of light

iris diaphragm

coarse adjustment knob

Unanswered
Question 43 0 / 1 pts

A student is looking at a bacterial specimen using the oil immersion lens, but has forgotten to put
immersion oil on the slide. How will the specimen appear?

Smaller than it would if immersion oil was used

Larger than it would if immersion oil was used

Correct Answer Somewhat fuzzy and have poor resolution

The same as it would if the immersion oil was used

To have no color

Unanswered
Question 44 0 / 1 pts

What is the purpose of ocular lens?

Improve resolution

Correct Answer Magnify the image from the objective lens

Decrease the refractive index

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Increase the light

Decrease the light

Unanswered
Question 45 0 / 1 pts

What does the term resolution refers to in microscopy?

Correct Answer It refers to the ability to distinguish fine structure and detail in a specimen.

It is the same as the total magnification of a specimen.

It is improved when longer wavelengths of light are employed.

It is only observed in stained specimens.

It refers to magnification when using the electron microscope.

Unanswered
Question 46 0 / 1 pts

If a patient had a bacterial infection that was not syphilis but was tested for syphilis using the FTA-ABS
test, what would the technician observe?
 Fluorescent cells on a dark background

Correct Answer No fluorescence in the patient sample

Fluorescence of a different color than expected

Three-dimensional host tissue cells with organelles clearly visible

Brightly colored three-dimensional bacterial cells

Unanswered
Question 47 0 / 1 pts

An acidic dye ________.

has a positively charged chromophore

has a negatively charged chromophore

is used to stain the background in a negative stain

is used to stain nuclei

Correct Answer has a negatively charged chromophore and is used to stain the background in a negative stain

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Unanswered
Question 48 0 / 1 pts

Immersion oil is necessary when using the 100x objective on a compound light microscope ________.

to lubricate the objective lens

to prevent the slide from scratching the lens

otherwise the image will be fuzzy

otherwise too much light is lost to refraction

Correct Answer
otherwise too much light is lost to refraction and the image will be fuzzy

Unanswered
Question 49 0 / 1 pts

Which of the following measures demonstrates better resolution?

Measuring two bacteria arranged end to end as individually having a length of 2 μm

Measuring two yeast cells as individually having a diameter of 10 μm

Correct Answer
Observing two adjacent bacteria as individually having a width of 0.3 μm

Observing a clump of algae 0.5 mm by 0.75 mm

 Measuring a flea as 1.2 mm at the longest point

Unanswered
Question 50 0 / 1 pts

Which two staining techniques employ a mordant?

Correct Answer Gram stain and flagella stain

Gram stain and acid-fast stain

Simple stain and acid-fast stain

Capsule stain and flagella stain

Gram stain and capsule stain

Unanswered
Question 51 0 / 1 pts

The counterstain used in the Gram stain is a basic dye.

Correct Answer True

False

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Unanswered
Question 52 0 / 1 pts

If a Gram stain is performed correctly, gram-negative bacteria appear colorless when viewing by a light
microscope.

True

Correct Answer
False

Unanswered
Question 53 0 / 1 pts

If a Gram stain is performed correctly, gram-positive bacteria appear purple in color when viewing by a
light microscope.

Correct Answer True

False
Unanswered
Question 54 0 / 1 pts

If acid-fast bacteria are stained with the Gram stain, they will stain gram-negative.

True

Correct Answer False

Unanswered
Question 55 0 / 1 pts

The limit of resolution of the compound light microscope is approximately 0.2 μm.

Correct Answer True

False

Unanswered
Question 56 0 / 1 pts

Either the phase-contrast microscopy or the differential interference contrast microscopy can be used to
view the internal structures of cells without staining.

Correct Answer

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True

False

Unanswered
Question 57 0 / 1 pts

Cells viewed with a darkfield microscopy appear similar to those stained with the negative stain.

Correct Answer True

False

Unanswered
Question 58 0 / 1 pts

The capsules and flagella of bacteria can be observed by gram-stained smears.

True

Correct Answer False

Unanswered
Question 59 0 / 1 pts

The greater resolution of the electron microscope compared to the compound light microscope is due to
the longer wavelengths of the electrons used to examine specimens.

True

Correct Answer
False

Unanswered
Question 60 0 / 1 pts

Scanning electron microscope is used to examine fine detail of molecular complexes, such as blood clots,
or molecules, such as DNA.

Correct Answer True

False

Unanswered
Question 61 Not yet graded / 1 pts

In 1877, Robert Koch thought preparing permanently stained slides would be valuable. Why was his
assessment correct?

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Your Answer:

Unanswered
Question 62 Not yet graded / 1 pts

In 1884, Hans Christian Gram described a method of staining bacterial cells while not staining surrounding
animal tissues. However, he thought that the staining method he developed was faulty because not all
bacteria stained. In a letter to the editor of the journal in which Gram published his findings, write your
response to Gram's concern.

Your Answer:

Unanswered
Question 63 Not yet graded / 1 pts

You are working in a clinical laboratory, and you need to examine an unstained urine sample for the
presence of bacteria. What type of light microscope should you use to observe this specimen? Explain
your answer.

Your Answer:

Unanswered
Question 64 Not yet graded / 1 pts
 Differentiate the Gram stain and the acid-fast stain.

Your Answer:

Unanswered
Question 65 Not yet graded / 1 pts

What are the similarities between brightfield-, darkfield-, phase-contrast-, and fluorescence microscopes?

Your Answer:

Quiz Score: 0 out of 65