d anscription

ONA-RNA

-Polymerase
metalloenzyme
⑧
enzyme
·

cor
↳ (2zu7
&2 BB' w
associates with
holo
-
RNA polymeras
enzyme
~

↳
identities promoter
to
to
from periniation
<PIC)
compelt

identifies
functions- a promoter region
② opens up a small
segment of dupley DNA

* B Polymerization
④ Identification of termination
signal
⑥ Responds
to activation andrepression.

Comeregion
(sense) in RNA-s Uracil replaces
strian down
stand
coding,
- -

thymine
5. As
Except in s

-
5:
3
Template
cantisense)
submit
TTGACA If
is

Eintracts
I
this ing

[
this
-
35 untered around-35

wir 10 TAT A AT -> concensus req.

(not exactly
-

same

upelement
40, -60 in each
organiam)
-

X
Supstram)

transcription
optimum
↳
distance be bubble
by
be conditioned
*ofactor can as
catalyst
an
<it isnot partof RNApolymeran)
integral
·
identifies the
promoter region another
combine with
·
After 9-10 bp are transcribed, it detaches I can
polymean
·
multiple types of a factors (in bacterial
promoter recognition specificity of
&binding diffrents at time
to on a modifies
RNA

polymerase

Be
promoters
approx
40 nucleotides -turns)
(YONA long

me
two short
sequences

·
· low

ease
E
upstream
melting temp
dissociation
transcription
start
site

became
->

downstream

of 14
strands
b/w DNA
content

Once RNA polymerace

 cloud open promotor compter complex
to

17bp separation
(maintained throughout

RNA
·

polymeras processive
is

low DNA
catalytic efficiency
than
polymerase
·

·
Have some 5'-s'exonuclease
actively proofreading)
some

· I in 104-105 error rate - more error

prove. Why?
 of
half life RNA-s
very (dutroyed.
less.

protein,defective proteins
end result is an also
destroyed
check pointmechanisms.
by some

Termination-
-

RNA
termination signal commences from
In
prokaryotes,
itself.
in
↳a region followed by AT rich
region coding rand
U. in mRNA.
ace followed by poly
dA and U (ribouracil)-weakest linkage
Ideoxy A)

of
termination
production
- RNA this
to
due

era

Grabert
ein olgen is
RNA ent

Tumination of hairpin loop

Go rich region-paused
followed by dArU-weak intraction -
falling or
 -
N

(formation of hairpin loop with attachmentof FMN]

nite initiate transcription?
RNAP find correct
to
How does

Itscans DNA seg

ata rate
of 103bp/s, until it

DNA to
which itbinds with higher
recognises
a
specific N

promoters
↓

affinity
promotor recognition-utilization process
a
regulatory step.

Riboswitch
-

Bacillus Subtilis

responsible for enzymes of riboflavin