Scribd
Upload
38 views2 pages

Mass Spectroscopy: Techniques and Applications

Mass Spectroscopy (MS) is an analytical technique that can determine the size and fragmentation patterns of molecules. MS is used to determine the structure of unknown compounds by analyzing their fragmentation patterns. It can also determine the purity of samples and monitor the results of chemical syntheses. The technique works by ionizing sample molecules and separating the ions by mass using electric and magnetic fields. Biochemists use MS for applications like determining accurate molecular weights, monitoring enzyme reactions, amino acid sequencing, and oligonucleotide sequencing.

Uploaded by

Non Linear
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
38 views2 pages

Mass Spectroscopy: Techniques and Applications

Mass Spectroscopy (MS) is an analytical technique that can determine the size and fragmentation patterns of molecules. MS is used to determine the structure of unknown compounds by analyzing their fragmentation patterns. It can also determine the purity of samples and monitor the results of chemical syntheses. The technique works by ionizing sample molecules and separating the ions by mass using electric and magnetic fields. Biochemists use MS for applications like determining accurate molecular weights, monitoring enzyme reactions, amino acid sequencing, and oligonucleotide sequencing.

Uploaded by

Non Linear
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Mass Spectroscopy (MS)

Objective: - MS is an analytical technique that can be used to determined the size and fragmentation pattern of molecules - MS is used to determine sizes of unknown compounds leading to illumination of their structure - MS can be used to determine the results of synthesis by the analysis of the fragmentation patterns of the compounds - MS can be used to determine the purity of samples Principle: - the respective compounds are brought into the gas phase and are charged usually due to fragmentation to become ions - the particles are then accelerated via and magnetic field and then deflected by an additional magnet - the degree of deflection depends on the mass/charge-ration of the fragment-ions - the relative ration are measured and used to determine the size and fragmentation pattern

Rules: -

fragmentation follows simple and predictable chemical pathways fragments represent the most stable cations and radicals highest molecular weight peaks is the molecular ion generally small peaks are observed above the calculated mass due to natural isotopic abundance the isotope pattern also provides information of analyte composition

Biochemists and MS: - Accurate molecular weight measurements: sample confirmation, to determine the purity of a sample, to verify amino acid substitutions, to detect post-translational modifications, to calculate the number of disulphide bridges - Reaction monitoring: to monitor enzyme reactions, chemical modification, protein digestion - Amino acid sequencing: sequence confirmation, de novo characterisation of peptides, identification of proteins by database searching with a sequence "tag" from a proteolytic fragment - Oligonucleotide sequencing: the characterisation or quality control of oligonucleotides - Protein structure: protein folding monitored by H/D exchange, protein-ligand complex formation under physiological conditions, macromolecular structure determination Example: - MS is used in many paper to confirm the results and the purity of synthesis of chemical compounds used further on (e.g. Mattner et al., Nature 434 (2005), p.525-529 to confirm the structure of the Sphingomonas lipids synthesized by them) References: [Link] [Link]

You might also like