Absorbance Spectrum Analysis of Potassium Permanganate
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Sarah B - she herAbsorbance Spectrum Analysis of Potassium Permanganate
Uploaded by
Sarah B - she herName: Sarah Bustin
Part A -Absorbance Spectrum of a Compound
How does varying the path length affect absorbance?
Absorbance increases with increasing path length.
What is the path length for your setup? (this should be constant through the whole lab).
0.50cm
How does varying the Concentration affect absorbance?
Absorbance increases with increasing concentration.
Which compound did you choose to prepare an absorbance spectra?
Potassium permanganate
What concentration did you choose?
500 micromoles
Fill out the table below. Please convert %Trans to Abs. to obtain the appropriate level of significance.
Wavelength %Trans. Absorbance Wavelength %Trans. Absorbance
380nm 77.96 0.1081 590nm 83.48 0.0784
390nm 78.11 0.1073 600nm 87.02 0.0604
400nm 83.29 0.0794 610nm 88.74 0.0519
410nm 86.08 0.0651 620nm 89.56 0.0479
420nm 87.10 0.0600 630nm 90.52 0.0433
430nm 87.12 0.0599 640nm 91.58 0.0382
440nm 85.68 0.0671 650nm 92.54 0.0337
450nm 81.76 0.0875 660nm 93.36 0.0298
460nm 76.12 0.1185 670nm 94.12 0.0263
470nm 69.16 0.1601 680nm 94.66 0.0238
480nm 59.67 0.2242 690nm 95.26 0.0211
490nm 51.72 0.2863 700nm 95.88 0.0183
500nm 42.88 0.3677 710nm 96.46 0.0157
510nm 38.09 0.4192 720nm 97.06 0.0130
520nm 33.50 0.4750 730nm 97.66 0.0103
530nm 33.16 0.4794 740nm 98.05 0.0086
540nm 32.61 0.4866 750nm 98.37 0.0071
550nm 35.34 0.4517 760nm 98.76 0.0054
560nm 46.63 0.3313 770nm 99.02 0.0043
570nm 51.85 0.2853 780nm 99.01 0.0043
580nm 70.00 0.1549
Absorbance vs. Wavelength
0.6000
0.5000
0.4000
Absorbance
0.3000
0.2000
0.1000
0.0000
370 420 470 520 570 620 670 720 770
Wavelength
Part B – Preparation of a Calibration Plot
What wavelength did you choose to prepare your Calibration Curve?
540nm
Choose 10 spread out concentrations and measure their % Transmittance at the selected
wavelength. Be sure that any % Transmittance readings are above 10%
Concentration (micromoles) % Trans. Absorbance
30 93.51 0.0291
140 73.09 0.1361
205 63.09 0.2000
270 54.58 0.2630
335 47.21 0.3260
380 42.66 0.3700
425 38.54 0.4141
490 33.34 0.4770
535 30.13 0.5210
800 16.62 0.7794
Using a graphing program, prepare a calibration plot for your data of Abs. vs. Concentration.
Absorbance vs. Concentration
0.9000
0.8000
0.7000
0.6000
0.5000
Absorbance
0.4000
0.3000
0.2000
0.1000
0.0000
0 100 200 300 400 500 600 700 800 900
Concentration
What should the absorbance be at a concentration of 0.000M?
0
What is the slope of your calibration plot?
9.740*10^-4
Using your measured pathlength, what is the Molar Absorptivity Constant for your compound
at this wavelength? Show your work.
Abs = εlc
L = 0.50cm = 0.0050m
C = 380 micromoles
Abs = 0.3700
ε = l*c/Abs = [0.0050(380)]/0.3700 = 5.1
At an absorbance of 0.111, what would be the concentration of your compound? Show your
work.
C = Abs/l*ε
C = 0.111/0.0050*5.14 = 110 micromoles
At a concentration of 0.221mM (use 0.221µM if you chose the chromate, dichromate, or
permanganate), what would be the absorbance of your compound? Show your work.
Abs = εlc = 5.14 * 0.0050 * 0.221 = 0.0057
Questions
What is the molecular weight of your compound?
158.034 g/mol
What is the maximum concentration allowed in the software for your compound?
800 micromoles
You wish to prepare 100.00mL of stock solution at that concentration. How many moles of
compound are needed? Show your work.
M = mol/L
Mol = L*M
0.01L * 800 micromoles = 8 micromoles
What mass of solid compound would you need to weigh out? Show your work.
800 * 10^-6 mol * 158.034g/mol = 0.1g
What was the lowest concentration you used in your calibration graph?
30 micromoles
What was the highest concentration you used in your calibration graph?
800 micromoles
What volume of stock solution would you need to prepare 100.00mL of your lowest
concentration? Show your work.
M1*V1=M2*V2
V1 = (M2*V2)/M1
M1 = mol/L1 = 800 * 10^-6 mol /0.01L = 0.08M
M2 = mol/L2 = 30 * 10^-6 mol/ 0.01L = 0.003M
V1 = (0.003M*0.01L)/0.08M = 0.000375L = 0.4mL
What volume of stock solution would you need to prepare 100.00mL of your highest
concentration? Show your work
100mL (see above)
How many grams of compound would be contained in a 100.00mL solution, which was
prepared using 25mL of Stock Solution? Show your work.
M1*V1 = M2*V2
M2 = (M1*V1)/V2 = (0.08M*0.0025L)/0.01L = 0.002M
M = mol/L
Mol = L*M = 0.01L * 0.002M = 0.0002 mol = 20 micromoles
20 * 10^-6 mol * 158.034g/mol = 0.003g