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Feasibility of Recyclable Ohmic Heater

The document describes a research study that aimed to create an alternative ohmic heater out of recyclable materials to kill food pathogens (Escherichia coli and Staphylococcus aureus). The alternative ohmic heater and an oven were tested at different temperatures (60°C, 75°C, 90°C) on the bacteria. Results showed that there was no significant difference in the effectiveness of the alternative ohmic heater and oven at killing the food pathogens. Therefore, the researchers concluded that the alternative ohmic heater is a feasible option for killing food pathogens.

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0% found this document useful (0 votes)
150 views11 pages

Feasibility of Recyclable Ohmic Heater

The document describes a research study that aimed to create an alternative ohmic heater out of recyclable materials to kill food pathogens (Escherichia coli and Staphylococcus aureus). The alternative ohmic heater and an oven were tested at different temperatures (60°C, 75°C, 90°C) on the bacteria. Results showed that there was no significant difference in the effectiveness of the alternative ohmic heater and oven at killing the food pathogens. Therefore, the researchers concluded that the alternative ohmic heater is a feasible option for killing food pathogens.

Uploaded by

ChricellF
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Barotac Viejo National High School

Senior High School


Barotac Viejo, Iloilo

The Feasibility of Making an Alternative Ohmic Heater Out of Recyclable Materials in Killing Food

Pathogens (Escherichia coli and Staphylococcus aureus)

A Research Paper Presented to the Faculty of Senior High School of

Barotac Viejo National High School

Barotac Viejo, Iloilo

In Partial Fulfillment of the Requirements

for Inquiries, Investigations and Immersion

Rogen Babiera Erna Angelika Gumaquil

Norman Baldevarona Jr. Angel Ann Manalo

Laiza Barrameda Cherry Anne Pabiona

Chelsea Ann Bonio Jenefer Villalobos

Angel Carnate II

April 2018
Barotac Viejo National High School
Senior High School
Barotac Viejo, Iloilo

Approval Sheet

This Research Paper entitled “The Feasibility of Making an Alternative Ohmic Heater Out of
Recyclable Materials in Killing Food Pathogens (Escherichia coli and Staphylococcus aureus).”
prepared and submitted by Rogen A. Babiera, Norman S. Baldevarona Jr. Laiza D. Barrameda,
Chelsea P. Ann Bonio, Angel D. Carnate II, Erna Angelika C. Gumaquil, Angel Ann B. Manalo,
Cherry Anne B. Pabiona, Jenefer C. Villalobos, in partial fulfillment of the requirements for
Inquiries, Investigations and Immersion is hereby accepted.

HILCRIS GIDEON F. ESLABRA HERNAN B. GUMAQUIL


Research Adviser and Statistician English Critic

(Date Signed) (Date Signed)

CLARK B. BANABATAC Ph.D. LEDENILA R. CAGUAN


Research Teacher Asst. School Principal II-Academics

(Date Signed) (Date Signed)

Accepted as partial fulfillment of the requirements for Inquiries, Investigations and

Immersion subject of Science Technology Engineering and Mathematics of Barotac Viejo

National High School.

WELLET A. DEL CASTILLO, Ed. D.


Principal IV

(Date Signed)

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Babiera, Rogen A., Baldevarona, Norman S. Jr.,Barrameda, Laiza D.,Bonio, Chelsea Ann
P.,Carnate, Angel D. II, Gumaquil, Erna Angelika C., Manalo, Angel Ann B., Pabiona, Cherry
Anne B., Villalobos, Jenefer C.,“The Feasibilty of Making Alternative Ohmic Heater out of
Recyclable Materials in Killing Food Pathogens (Escherichia coli and Staphylococcus aureus)”
Unpublished Research Paper. Senior High School,Barotac Viejo National High School. Barotac
Viejo Iloilo, April 2018.

Abstract

This study used experimental research to identify the feasibility of making an Alternative Ohmic

Heater out of recyclable materials in killing food pathogens. In this study the researchers used

(Escherichia coli and staphylococcus aureus). This study aimed to create an alternative ohmic

heater used to kill food pathogens. The materials used in the construction of ohmic heater are

all recyclable materials, and can resist the process temperature. The tank is the site where the

treatments were placed, with different temperature points (60oC, 75oC, and 90oC) were used to

test the effectiveness of ohmic heater in killing food pathogens, and also the same temperature

points were used in oven. The bacteria were sterilized using the autoclave at 121 oC per square

inch at 15 minutes. Using the 0.5 Mc Farland standards with 1.5x10 8, the E. coli and S. aureus

were inoculated unto sterile. The inoculated culture plates were exposed for 1 minute under the

ohmic heater, same procedure used for the oven. A total 36 petri dishes were incubated at 35 oC

to 37oC aerobically for 18 to 24 hours. The results were obtained by counting the aerobic plate

growth of bacteria.

Using the kruskal-wallis test, the result revealed that the alternative ohmic heater is the

same compared to the oven in killing the food pathogens. Hence, the researchers concluded

that there is no significant difference in the effect of alternative ohmic heater and oven in killing

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food pathogens. This stuidy will able to help the community to lessen the harmful s caused by

the food pathogens.

Rogen A. Babiera

Norman S. Baldevarona Jr.

Laiza D. Barrameda

Chelsea P. Ann Bonio

Angel D. Carnate II

Erna Angelika C. Gumaquil

Angel Ann B. Manalo

Cherry Anne B. Pabiona

Jenefer C. Villalobos

April 2018

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Acknowledgement

Immeasurable appreciation and deepest gratitude for the help and support extended to us

by the following persons making this study possible.

First of all to the Almighty God, who gave us strength, will and knowledge in order to

accomplish this study.

Dr. Wellet A. Del Castillo, Principal IV of Barotac Viejo National High School for allowing

the researchers to conduct their study at Janiuay Comprehensive National High School, for the

opportunity and support given to the researchers.

Dr. Clark B. Banabatac, research teacher, for his support, words of encouragement and for

his time and patience rendered to the researchers.

Mr. Hilcris Gideon F. Eslabra, research adviser and statistician for his advices, suggestions,

comments and provision that benefited the researchers much in completion and success of this

study. Sharing his knowledge and helped in the analysis of data and its statistical computations.

Mr. Hernan B. Gumaquil, English critic, for his effort and time in correcting the grammatical

error of the papers.

Mr. Felix Delos Santos and Mr. Gerome Rea, ICT teachers, for shared an idea to the

researchers about the ohmic heater process.

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Ms. Jia Marie E. Balayo, 12 STEM Adviser, for understanding the researchers in sharing her

time for the completion of this study.

To all the parents of the researchers, for understanding, support, giving time and effort

especially for the financial needs of the researchers.

And to the other people who really supported and helped the researchers to make this

study a successful one.

Rogen A. Babiera

Norman S. Baldevarona Jr.

Laiza D. Barrameda

Chelsea P. Ann Bonio

Angel D. Carnate II

Erna Angelika C. Gumaquil

Angel Ann B. Manalo

Cherry Anne B. Pabiona

Jenefer C. Villalobos

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Table of Contents

Page

Title Page i

Approval Sheet ii

Abstract iii

Acknowledgement v

Table of Contents vii

List of Tables x

List of Figures xi

Chapter I

INTRODUCTION OF THE STUDY 1

Background of the Study 2

Statement of the Problem 4

Conceptual Framework 4

Hypotheses 5

Significance of the Problem 5

Definition of terms 6

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Page

Scope and Limitation of the Study 8

Chapter II

Review of Related Literature 9

Ohmic Heater 10

Escherichia coli 11

Staphylococcus aureus 12

Review of Related Studies 12

Ohmic Heating as an Alternative Food Processing Technology 12

Effect of Ohmic Heating of Buffalo Milk on Microbial Quality and Tesure of Paneer 13

Effects of Ohmic Heating, Including Electric Field Intensity and Frequency on

Thermal Inactivation of Bacillus subtilis Spores 14

Chapter III

METHODOLOGY 15

Research Design 15

Preparation of Materials and Equipment 16

Development of Ohmic Heating Setup 16

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Page

Culturing and Preparation of Bacteria 17

Data and Statistical Procedure 18

Statistical Data Analysis Procedure 19

Chapter IV

RESULT AND DISCUSSION 22

CHAPTER V

SUMMARY, CONCLUSION AND RECOMMENDATION 27

Summary 27

Conclusion 28

Recommendation 29

REFERENCES 30

APPENDICES 32

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List of Tables

Page

Table

1. Total Aerobic Plate Count (Colony Forming Units (cfu ) ) in microliter of

Escherichia coli using Alternative Ohmic Heater as experimental group

and commercial electric oven as control group. 20

2. Total Aerobic Plate Count (Colony Forming Units (cfu ) ) in microliter of

Staphylococcus aureus using Alternative Ohmic Heater as experimental group

and commercial electric oven as control group. 21

3. Total Aerobic Plate Count (Colony Forming Units (cfu ) ) in microliter of

Escherichia coli using Alternative Ohmic Heater as experimental group

and commercial electric oven as control group. 22

4. Total Aerobic Plate Count (Colony Forming Units (cfu ) ) in microliter of

Staphylococcus aureus using Alternative Ohmic Heater as experimental group

and oven as control group. 24

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List of Figures

Page

Figure

1. Conceptual Framework

The relationship of the effect of Ohmic Heater to food Pathogens. 4

2. Schematic Flow chart of the construction of alternative Ohmic heater

until to the application and statistical analysis of the data. 19

xi

Common questions

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The study measured the effectiveness of the alternative ohmic heater by counting aerobic bacterial growth on petri dishes after treatment. Total aerobic plate counts in Colony Forming Units (cfu) provided a quantitative measure of bacterial reduction, allowing for direct comparison between the ohmic heater and a commercial oven. This data was analyzed statistically to assess the significance of differences in bacterial counts between the two methods .

The study's approach aligns with current trends in sustainable technology development by leveraging recyclable materials to create an innovative and environmentally conscious solution. This reflects the broader trend of utilizing waste products to minimize environmental impact, promoting circular economy principles, and addressing sustainability challenges through technological advancements. Such approaches are increasingly significant as global industries prioritize sustainable resource utilization .

Using recyclable materials in constructing the alternative ohmic heater has several implications. It promotes environmental sustainability by reducing waste and resource consumption. Additionally, it provides an innovative approach to designing cost-efficient and eco-friendly technology for food pathogen reduction. This approach taps into resourcefulness in material use, which can inspire further innovations in various fields, particularly those seeking sustainable technological solutions .

The study suggested that the alternative ohmic heater could help the community by providing a cost-effective and environmentally friendly solution to reduce harms caused by food pathogens. By using recyclable materials, the heater contributes to sustainability and potentially reduces costs associated with more expensive sterilization equipment like commercial ovens .

The study aimed to create an alternative ohmic heater using recyclable materials to kill food pathogens like Escherichia coli and Staphylococcus aureus. It involved testing the effectiveness of the ohmic heater at different temperature points and comparing it with an oven to determine its feasibility as an alternative sterilization method using recyclable materials .

The study concluded that there was no significant difference in the effectiveness of the alternative ohmic heater compared to a commercial oven in killing food pathogens. Both methods were similarly effective in sterilizing the bacteria, indicating the potential of the ohmic heater as a viable alternative heating method for reducing food pathogens .

The experimental design involved inoculating culture plates with Escherichia coli and Staphylococcus aureus at 0.5 McFarland standards. The plates were exposed at different temperature points (60°C, 75°C, 90°C) for one minute under both the ohmic heater and an oven. These were then incubated at 35°C to 37°C aerobically for 18 to 24 hours. The results were analyzed using the Kruskal-Wallis test to determine if there was a significant difference in the effectiveness of the ohmic heater compared to the oven in reducing bacteria growth .

In scaling the alternative ohmic heater for wider use, researchers might face challenges such as ensuring uniform heat distribution in larger units, maintaining the efficiency and reliability proven in smaller scale experiments, and sourcing sufficient quantities of uniform quality recyclable materials. Additionally, regulatory approvals and technology adoption in diverse settings might pose significant hurdles. Adequate safety measures will also need to be designed for larger-scale applications .

The Kruskal-Wallis test was chosen because it is a non-parametric method suitable for comparing more than two independent groups when the data do not necessarily follow a normal distribution. In this study, the test was used to compare the effectiveness of the alternative ohmic heater and the oven in killing food pathogens, providing a robust statistical analysis despite potential non-normal data distributions .

The researchers standardized treatment conditions by using specific temperature settings (60°C, 75°C, and 90°C) and consistent exposure times of 1 minute for both the ohmic heater and the oven. Furthermore, the inoculated culture plates were incubated under controlled aerobic conditions at a temperature range of 35°C to 37°C for the same duration across experiments to ensure consistency in experimental conditions .

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