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Osmosis Experiments Using Visking Tubing

The document outlines several biology experiments and practical activities covering topics like osmosis, effects of osmosis on red blood cells, measuring the energy content of foods, dissections of organs from various organisms, identification of microorganisms and plant and animal cells/tissues under the microscope. The experiments are designed to demonstrate osmosis, observe changes in blood cells in different solutions, calculate energy transferred from burning foods into water, examine structures of organs and differentiate between types of microbes, plant and animal structures.

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Zulaikha Chalki
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0% found this document useful (0 votes)
197 views17 pages

Osmosis Experiments Using Visking Tubing

The document outlines several biology experiments and practical activities covering topics like osmosis, effects of osmosis on red blood cells, measuring the energy content of foods, dissections of organs from various organisms, identification of microorganisms and plant and animal cells/tissues under the microscope. The experiments are designed to demonstrate osmosis, observe changes in blood cells in different solutions, calculate energy transferred from burning foods into water, examine structures of organs and differentiate between types of microbes, plant and animal structures.

Uploaded by

Zulaikha Chalki
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

🧬

BIOLOGY PRACTICAL
Experiments
Experiment on the effect of osmosis using Visking Tubing
Title: Experiment on the effect of Osmosis using Visking Tubing
Hypothesis: If water molecules move into the Visking tubing by osmosis, then the
weight of the tubing will increase.
Required materials: Visking tubing, beaker, pipette, sucrose solution, distilled
water, rubber ring, balance

Procedure:

1. Cut the Visking Tubing at about 15 cm.

2. Soften the Visking Tubing under running water until it opens up.

3. Tie a knot at one end to make a bag.

4. Fill the bag with sucrose solution using a pipette.

5. Tie the other end of with a rubber band and measure the initial weight using
an electronic balance.

6. Put the tubing into a beaker of distilled water.

7. Leave for about 15 minutes and measure the final weight.

Results: Water moves by osmosis from the high water concentration (dilute
solution) in the beaker into the low water concentration (concentrated solution) in
the Visking tubing across the membrane, increasing the volume of liquid in the
Visking tubing, which forces liquid up the capillary tube.

Experiment on the effects of osmosis on RBC


Title: Experiment on the effects of osmosis on Red Blood Cells

BIOLOGY PRACTICAL 1
Hypothesis: If blood cells are placed in a hypertonic solution, the water molecules
will move out of the cells into the solution by osmosis. If blood cells are placed in a
hypotonic solution, the water molecules will enter the cells from the solution by
osmosis. If the blood cells are placed in an isotonic solution, then osmosis will not
occur.
Required Materials: Blood sample, distilled water, NaCl solution (of different
concentrations), test tubes, microscope
Procedure:

1. Set up three test tubes labelled A, B and C containing the following solutions:

-A= 5cm3 of distilled water


-B= 5cm3 of 0.85% salt solution
-C= 5cm3 of 5% salt solution

2. Add 0.5cm3 of blood to all the test tubes and shake the test tubes.

3. Take a sample from each tube and examine it under the microscope.

4. Compare the blood cells in the three test tubes.

Result: The blood cells placed in the hypotonic solution (distilled water) swelled and
bursted, also called hemolysis. The blood cells placed in the isotonic solution ( 0.85
salt solution) remained the same. The blood cells placed in the hypertonic solution
(5 salt solution) shrank with crinkly edges.

Conclusion: In a hypotonic solution, blood cell membranes burst due to water


entering the cells by osmosis and have no cell wall like plant cells. In an isotonic
solution, the blood cells and the solution have the same concentration so osmosis
did not occur. In a hypertonic solution, the concentration of the blood cell is lower
than that of the salt solution, so the water from the cell moved out to the
concentrated solution by osmosis.

Investigation of the effect of exercise on breathing rate

Title: Experiment to investigate on the effect of exercise on breathing rate


Hypothesis: If exercise intensity increases, then the breathing rate is increased.

Required material: Students, Stopwatch

Procedure:

BIOLOGY PRACTICAL 2
1. Count the number of breaths for 2 subjects in one minute while at rest.

2. Repeat three times and record the result in a table.

3. Perform some vigorous exercises, such as running on a treadmill for 3


minutes.

4. Record the breathing rate per minute until they return to their normal resting
rate.

5. Plot a line graph of these results, using the same axes for both subjects.

6. Join the data points using straight lines.

7. Explain why the breathing rate needs to rise during exercise and describe
which subject is more fit.

Result: (in table)

Conclusion: After exercise, the breathing rate in increased in both students. This is
because more energy and oxygen is needed during exercise. Student _ is more fit
than Student _ as the breathing rate returned to normal at a faster rate compared to
subject _.

Experiment to measure the energy content in a food sample


Title: Experiment to measure the energy content in a food sample

Hypothesis: If energy stored in food sample is transferred to water, then


temperature of the water will rise by food energy.
Required materials: Food sample, electronic balance, thermometer, mounted
needle, water, Bunsen Burner, retort stand, boiling tube

Procedure:

1. Measure the mass of the food sample on a balance.

2. Place 20cm3 of water in a boiling tube and support the tube on a clamp on
a stand as shown in the diagram.

3. Measure the initial temperature of water using thermometer.

4. Hold the food sample on the end of the mounted needle and on a Bunsen
burner flame until it catches fire.

BIOLOGY PRACTICAL 3
5. Hold the mounted needled with burning food underneath the boiling tube of
water so that the flame heats up the water.

6. Measure the final temperature of the water when the food is not longer
burning. Stir the thermometer.

7. Calculate the amount of energy transferred to the water using the formula
below.

Conclusion: Energy= (final temp-initial temp) * 20 * 4.2 / mass of food

Dissections
Brain of Fish

Brain of Chicken

Digestive System of Fish

Nervous System of Fish

Nervous System of Prawn

Afferent Branchial Artery

Identifications
Identifying Microorganisms

Amoeba

BIOLOGY PRACTICAL 4
Paramecium

BIOLOGY PRACTICAL 5
Euglena

BIOLOGY PRACTICAL 6
Yeast

BIOLOGY PRACTICAL 7
Mucor

BIOLOGY PRACTICAL 8
Bacteria s

BIOLOGY PRACTICAL 9
Respiratory system of a frog

Digestive system of a frog

BIOLOGY PRACTICAL 10
Identifying Monocots / Dicots /Guard Cells

BIOLOGY PRACTICAL 11
BIOLOGY PRACTICAL 12
Parts of a flower

BIOLOGY PRACTICAL 13
BIOLOGY PRACTICAL 14
Brain of Fish/Chicken

BIOLOGY PRACTICAL 15
4 Stages of Mitosis

Stage 1 and 2 of Meiosis

BIOLOGY PRACTICAL 16
BIOLOGY PRACTICAL 17

Common questions

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Through these experiments, students develop proficiency in using laboratory equipment, conducting systematic observations, understanding biological structures and functions, and applying scientific methods to solve complex questions. They also enhance critical thinking skills by analyzing data, interpreting results, and understanding the interconnections between theoretical knowledge and practical application .

Experiments on osmosis and exercise can significantly enhance the learning experience by providing hands-on understanding of complex concepts like selective permeability and physiological responses to stimuli. They encourage critical thinking, foster an empirical understanding of theoretical knowledge, and help students grasp the intricacies of biological processes through active participation and observation, which are critical in educational practices .

The experiment showcases osmosis by illustrating how water molecules move from an area of low solute concentration (the distilled water in the beaker) to an area of high solute concentration (the sucrose solution inside the Visking tubing). The semipermeable membrane of the Visking tubing allows water molecules, but not sucrose molecules, to pass through. This results in an increase in the volume of liquid inside the tubing, leading to a measurable increase in weight .

The Visking tubing experiment models natural cellular environments by simulating the plasma membrane's selective permeability. It demonstrates osmosis by allowing the movement of water but not solutes, akin to how cell membranes operate. This experiment illustrates vital cellular processes such as nutrient uptake and waste removal in an aqueous environment, fundamental to understanding cell physiology .

The stages of mitosis and meiosis are crucial for understanding cellular reproduction. Mitosis involves stages: prophase, metaphase, anaphase, and telophase leading to genetically identical cells, important for growth and repair. Meiosis, however, includes two series of phases (meiosis I and II), producing genetically diverse gametes, critical for sexual reproduction. These processes are fundamental in practical biology for grasping genetic stability and variation .

The increased breathing rate after exercise meets the heightened demand for oxygen to support elevated metabolic rates and to expel excess carbon dioxide produced by muscle activity. More fit individuals experience quicker recovery as their cardiovascular systems are more efficient at transporting oxygen and removing waste, hence the faster return to normal breathing rates .

In a hypotonic solution, such as distilled water, red blood cells absorb water due to the higher internal solute concentration, causing them to swell and potentially burst (hemolysis). In an isotonic solution (0.85% salt solution), there is no net movement of water as the solute concentration is balanced, maintaining cell shape. In a hypertonic solution (5% salt solution), water exits the cells, leading to crenation or shrinkage due to higher external solute concentration .

Understanding osmotic behavior in red blood cells is critical due to its implications for medical treatments, such as intravenous therapy, where the isotonic balance needs to be maintained to avoid hemolysis or crenation of cells. This knowledge also aids in designing solutions that manage fluid balance in clinical settings and for physiological understandings of cell environment interactions .

This experiment highlights that individuals with higher cardiovascular efficiency and physical fitness levels will return to their baseline breathing rate more quickly after exercise. A faster recovery rate demonstrates effective heart and lung capacity, indicating better oxygen delivery and carbon dioxide removal, which are hallmarks of enhanced fitness and endurance .

In this experiment, energy from a burning food sample is transferred to a known volume of water, raising its temperature. The scientific principle is based on the specific heat capacity of water, allowing calculation of energy absorbed using the formula: Energy = (final temperature - initial temperature) * 20 * 4.2 / mass of food. This method translates thermal energy into quantifiable data regarding the food's caloric content .

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