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Molecular Genetics and Bioinformatics Course

This document provides information about the Molecular Genetics and Bioinformatics course taught by Bjorn Johansson. It includes the instructor's contact information and office hours, as well as links to course materials hosted on Dropbox. The course literature covers 16 chapters on topics related to gene cloning and DNA analysis. Tests will evaluate both theoretical and practical bioinformatics concepts taught in classes. Students are required to have a laptop computer to complete technical practical assignments, which will involve in silico techniques like sequence analysis and molecular cloning simulations.

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Anita Torres
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11 views13 pages

Molecular Genetics and Bioinformatics Course

This document provides information about the Molecular Genetics and Bioinformatics course taught by Bjorn Johansson. It includes the instructor's contact information and office hours, as well as links to course materials hosted on Dropbox. The course literature covers 16 chapters on topics related to gene cloning and DNA analysis. Tests will evaluate both theoretical and practical bioinformatics concepts taught in classes. Students are required to have a laptop computer to complete technical practical assignments, which will involve in silico techniques like sequence analysis and molecular cloning simulations.

Uploaded by

Anita Torres
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Genética Molecular

& Bioinformática
2704N9
Docente

Hor. atend.: Sexta-feira 16:00 – 17:00


bjorn_johansson@[Link]
How do we define Molecular genetics and how does it relate to
bioinformatics?
Dropbox with *all* course files
[Link]

[Link]
[Link]
Schedule BA
Schedule BQ
Course literature

1 Why Gene Cloning and DNA Analysis are Important


2 Vectors for Gene Cloning: Plasmids and Bacteriophages
3 Purification of DNA from Living Cells
4 Manipulation of Purified DNA
5 Introduction of DNA into Living Cells
6 Cloning Vectors for E. coli
7 Cloning Vectors for Eukaryotes (7.1.1 – 7.1.5)
8 How to Obtain a Clone of a Specific Gene
9 The Polymerase Chain Reaction
10 Sequencing Genes and Genomes
11 Studying Gene Expression and Function
12 Studying Genomes (12.1.1-12.2.2)
13 Production of Protein from Cloned Genes
14 Gene Cloning and DNA Analysis in Medicine
15 Gene Cloning and DNA Analysis in Agriculture
16 Gene Cloning and DNA Analysis in Forensic Science
and Archaeology (not incuded)

16 chapters, ~320 pages ~ 20 pages per week

[Link]
TP classes (Mostly Bioinformatics)

● In silico sub-cloning, replicating a cloning


strategy on your computer
● PCR primer design
● PCR simulation
● Gibson assembly and homologous recombination
● Design CRISPR Cas9 guide RNAs
● Restriction mapping
● Sequence search and comparison by BLAST
Evaluation

T and TP classes are not mandatory


(but I will keep statistics on presence)
Theoretical Test 1 & 3
Test 2 (practical bioinformatics)
Computers for TPs

You *need* a laptop for all TPs.


(Tablet will not work)

Who has Windows?

Anyone has Mac?

Anyone has Linux?


Live quiz!

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