PU-4086-Binary

PU-4185-Binary
PU-4285-Binary
Binary Pump

Operator’s Manual

P/N:0302-2101A December 2014

Safety Considerations
To ensure operation safety, this instrument must be operated correctly and maintained
according to a regular schedule. Read carefully to fully understand all safety precautions in
this manual before operating the instrument. To avoid injuries or health hazards, strictly follow
the safety precautions mentioned in this manual. Please take a moment to understand what
the signal words WARNING!, CAUTION, and Note mean in this manual.

(1) Safety symbols

Instruction manual symbol. If the product is marked with this

symbol, refer to the instrument manuals to protect the instrument
against damage.

WARNING! A WARNING! indicates a potentially hazardous situation which,

if not avoided, could result in serious injury or even death.

CAUTION A CAUTION indicates a potentially hazardous situation which, if

not avoided, may result in minor or moderate injury. It may also
be used to alert against damaging the instrument.

Do not proceed beyond a WARNING! or CAUTION notice until

you understand the hazardous conditions and have taken the
appropriate steps.

Note A Note provides additional information to help the operator

achieve optimal instrument performance.

i
(2) Warning Label

Warning labels are attached at several locations on this instrument. Do not remove, deface or
damage the warning labels. If a warning label peels off the instrument or becomes illegible,
contact your local JASCO distributor and state the part number of the label you want to
replace.

1) Power Fuse and Ground Warning Label (see Figure 1)

P/N: 0822-0163A

Power fuse ratings are described on the rear

panel.
Electric shock may occur if the instrument is not
properly grounded.

2) High Voltage Warning Label (see Figure 1)

P/N: 0822-0110A

WARN ING A voltage of 200 V or greater is generated

during the operation of the pump. Always turn
DANGE ROUS VOLTAGE EXPOSED
off the power and disconnect the power cable
WHEN OVER IS REMOVED
before starting maintenance.

3) The crossed-out wheeled trash bin WEEE Label

P/N: 0822-0174A

This symbol [crossed-out wheeled trash bin WEEE] indicates separate collection of
waste electrical and electronic instrument in the EU countries.
Please do not throw the instrument into the domestic refuse.
Please use the return and collection systems available in your country for the disposal
of this product.

ii
 2) High Voltage Warning Label

3) The crossed-out wheeled

trash bin WEEE Label

1) Power Fuse and Ground

Warning Label

Figure 1 LC-4000 Series Pump Top Rear View

iii
(3) Warning for Carrying
This instrument weighs approximately 21.0 kg for the PU-4086-Binary, 20.5 kg for the
PU-4185-Binary, and 22.5 kg for the PU-4285-Binary. When carrying the instrument, be sure
to hold the instrument bottom firmly as shown in Figure 2.

Figure 2 How to Carry

The front door is made by resin and is not designed to carry weight. If you carry the instrument
with putting your hand on the bottom of the front door as shown in Figure 3, the door may be
broken. Always hold the instrument bottom for carrying as shown in Figure 2.
In addition, close the door when carrying the instrument.

Figure 3 Unsuitable Carrying

iv
(4) Warning for Solvent

Avoid using the following solvents or observe the recommendations on the use of solvents.

1) Stainless steel-corrosive solvents (alkali halides (fluoride, bromide, iodide, and their
salts) e.g., lithium iodide, potassium chloride, and sodium chloride).

2) High concentrations of inorganic acids (e.g. sulfuric acid and nitric acid).
(If it is necessary to use these solvents under your chromatographic conditions, use them
only in weak concentrations, typically less than 5 %, and clean the instrument with water
immediately after completing the analyses.)
3) Halogenated solvents or mixtures which form radicals and/or acids.

4) LC-grade ethers which contain peroxides (e.g., dioxane and di-isopropyl ether).

(If it is necessary to use these solvents, use them after being filtered through dry
aluminium oxide that adsorbs the peroxide.)

5) Solvents containing strong concentrations of complexing agents (e.g., ethylene

diaminetetraacetic acid (EDTA)).
6) Mobile phase solvents which deteriorate a column (e.g., solvents which pH is less than
1.0, in the case of silica-based C18 column).

7) Quarts glass is used for the flow cell window. Avoid using the solvents which attack this
material (e.g., high concentrations of alkaline solvent which pH is more than 9.5).

8) Solvents which attack other wetted materials (e.g., mixture of carbon tetrachloride and
2-propanol (IPA) or tetrahydrofuran (THF)).
9) Aqueous solvents should not remain in shut-down instruments for a long time. Molds or
algae may be produced, causing the clog of the system flow lines. Replace with the
solvents containing organic solvents or aqueous solvents containing organic solvent
such as methanol and acetonitrile.

Note: Solvents described in 1) can be used with the inert version of the instrument.

v
Connecting the power cable
WARNING!
The green/yellow ground core of the mains lead must be connected to a ground that
complies with the local electricity supply authority (or equivalent body). The instrument is
dangerous if not correctly grounded.

240 Volt (nominal) Supply

Wire Color
Ground Green/Yellow
Line Brown
Neutral Blue

115 Volt (nominal) Supply

Wire Color

Ground Green/Yellow
Line Black
Neutral White
View of pin side of plug

Note: Instruments intended for operation at 115 V, 60 Hz are supplied with a mains
cable with a molded plug and socket.

vi
Regulatory Statement

CE Notice
The symbol indicates compliance of this JASCO system to the EMC (Electromagnetic
Compatibility) and Low Voltage Directives of the European Community. This symbol indicates
that this JASCO system meets the relevant basic safety and health requirements of the EC
Directive based on the following technical standards:

 EN61326-1: "Electrical instrument for measurement, control and laboratory use – EMC
requirements – Part 1: General requirements"

WARNING!
This is a Class A product. In a domestic environment this product may cause radio
interference, in which case the user may be required to take corrective measures.

 IEC61000-3-2: "Electromagnetic compatibility (EMC) Part 3-2: Limits – Limits for harmonic
current emissions (instrument input current up to and including 16A per phase)"
 IEC61010-1: "Safety requirements for electrical instrument for measurement, control and
laboratory use – Part 1: General requirements"

 A "Declaration of Conformity" in accordance with the above standards has been made and
is on file at JASCO CORPORATION, 2967-5 Ishikawa-machi, Hachioji-shi, Tokyo
192-8537, JAPAN.

vii
viii
Preface
This instruction manual is your guide for using this instrument. It instructs first-time users on
how to use the instrument, and serves as a reference for experienced users.

Before using the instrument, please read this instruction manual carefully, and make sure that the
contents are fully understood. This manual should be easily accessible to the operator at all times
during instrument operation. When not using the instrument, keep this manual in a safe place. If
this instruction manual becomes lost, order a replacement from your local JASCO distributor.

Servicing
Contact your local JASCO distributor for instrument servicing. In addition, contact your
JASCO distributor before moving the instrument to another location. Consumable parts
should be ordered according to part number from your local JASCO distributor. If a part
number is unknown, give your JASCO distributor the model name and serial number of your
instrument.

Do not return contaminated products or parts that may constitute a health hazard to
JASCO employees.

ix
Installation Requirements
To ensure operation safety, observe the following conditions:

(1) Do not operate the instrument under voltage fluctuations exceeding 10% of the
recommended line voltage. Otherwise, the instrument may not function properly.
(2) Frequency or spike noise in the power supply should be minimal.

(3) Ensure that the instrument is grounded.

(4) Operate the instrument in a temperature range of 10 to 30 °C.

(5) Operate the instrument in a humidity range of 35 to 85% (RH). If ambient humidity
exceeds 85% (RH), condensation may deteriorate optical components.

(6) Operate the instrument in an atmospheric pressure range of 750 to 1060hPa.

(7) Avoid strong magnetic fields and sources of high frequency. The instrument may not
function properly when near a strong magnetic field or high frequency source.

(8) Avoid vibration from vacuum pumps, electric motors, processing instrument and
machine tools.

(9) Avoid dust and corrosive gas. Do not install the instrument where it may be exposed to
dust, especially in locations exposed to outside air or ventilation outlets that discharge
dust particles.

(10) Do not install the instrument in a location where it may be exposed to direct sunlight.

(11) Install the instrument in a horizontal and stable position. (This includes a table or desk
upon which the instrument is installed.)

(12) Ensure that no air conditioner blows air directly onto the instrument. This may prevent
stable measurement.
(13) Install the instrument in a location that ensures sufficient space around and beneath it
for efficient air circulation and allows easy access for maintenance.

Note: The above conditions do not guarantee optimal performance of this

instrument.

x
Notices
(1) JASCO shall not be held liable, either directly or indirectly, for any consequential
damage incurred as a result of product use.

(2) Prohibitions on the use of JASCO software

 Copying software for purposes other than backup

 Transfer or licensing of the right to use software to a third party
 Disclosure of confidential information regarding software
 Modification of software
 Use of software on multiple workstations, network terminals, or by other methods
(not applicable under a network licensing agreement concluded with JASCO)

(3) The contents of this manual are subject to change without notice for product
improvement.

(4) This manual is considered complete and accurate at publication.

(5) This manual does not guarantee the validity of any patent rights or other rights.
(6) If a JASCO software program has failed causing an error or improper operation, this
may be caused by a conflict from another program operating on the PC. In this case,
take corrective action by uninstalling the conflicting product(s).
(7) Windows is a registered trademark of Microsoft Corporation in the United States and other
countries. In general, company names and product names are trademarks or registered
trademarks of the respective companies. However, the TM and ® marks are not used in
all cases in this manual.

(8) When disposing of the instrument, parts, and waste solvents, take appropriate
measures in compliance with applicable regulations regarding waste disposal, and
correctly dispose of them by yourself, or entrust disposal to a licensed industrial waste
disposal company. In any case, comply with the regulations in your country, state,
region or province to ensure they are disposed of legally and correctly.
(9) JASCO and the JASCO logo are registered trademarks of JASCO Corporation in Japan
and other countries.

(C) JASCO Corporation, 2014. All rights reserved. Printed in JAPAN.

xi
Limited Warranty
Products sold by JASCO, unless otherwise specified, are warranted for a period of one year
from the date of shipment to be free of defects in materials and workmanship. If any defects in
the product are found during this warranty period, JASCO will repair or replace the defective
part(s) or product free of charge.

THIS WARRANTY DOES NOT APPLY TO DEFECTS RESULTING FROM THE

FOLLOWING:

(1) IMPROPER OR INADEQUATE INSTALLATION

(2) IMPROPER OR INADEQUATE OPERATION, MAINTENANCE, ADJUSTMENT OR

CALIBRATION

(3) UNAUTHORIZED MODIFICATION OR MISUSE

(4) USE OF CONSUMABLE PARTS NOT SUPPLIED BY AN AUTHORIZED JASCO

DISTRIBUTOR

(5) CORROSION DUE TO THE USE OF IMPROPER SOLVENTS, SAMPLES, OR DUE

TO SURROUNDING GASES
(6) ACCIDENTS BEYOND JASCO'S CONTROL, INCLUDING NATURAL DISASTERS

This warranty does not cover the consumable parts listed below:

(1) Tungsten lamp, and other light sources

(2) Mirrors in the light source section, and cell windows

(3) Fuses, batteries, glassware, chart paper and ink

THE WARRANTY FOR ALL PARTS SUPPLIED AND REPAIRS PROVIDED UNDER THIS
WARRANTY EXPIRES ON THE WARRANTY EXPIRATION DATE OF THE ORIGINAL
PRODUCT. FOR INQUIRIES CONCERNING REPAIR SERVICE, CONTACT YOUR JASCO
DISTRIBUTOR AFTER CONFIRMING THE MODEL NAME AND SERIAL NUMBER OF
YOUR INSTRUMENT.

JASCO Corporation
2967-5, Ishikawa-machi, Hachioji-shi
Tokyo 192-8537
JAPAN

xii
Contents
Safety Considerations................................................................................................i
Connecting the power cable....................................................................................vi
Regulatory Statement..............................................................................................vii
Preface.......................................................................................................................ix
Servicing ...................................................................................................................ix
Installation Requirements.........................................................................................x
Notices.......................................................................................................................xi
Limited Warranty .....................................................................................................xii
Contents ..................................................................................................................xiii
1. Overview and Specifications..............................................................................1
1.1 Overview..................................................................................................................1
1.2 Specifications .........................................................................................................1
2. Parts and Functions............................................................................................7
2.1 Exterior....................................................................................................................7
2.2 Operation Panel ......................................................................................................8
2.3 Inside of the PU-4086-Binary ...............................................................................11
2.4 Inside of the PU-4185/4285-Binary.......................................................................12
2.5 Rear View ..............................................................................................................14
2.6 Input/Output Terminals ........................................................................................15
3. Installation .........................................................................................................16
3.1 Unpacking and Inspection ...................................................................................16
3.2 Installation.............................................................................................................18
3.3 Installing the Pump to LC System .......................................................................19
3.3.1 Attaching Drain Port (Bottom) or End Drain Port............................................19
3.3.2 LC System Examples ....................................................................................20
3.4 Wiring ....................................................................................................................22
3.4.1 Connecting Power Cable ...............................................................................22
3.4.2 Connecting LC-Net Cable in the LC System ..................................................22
3.4.3 Interlocking with Other Instruments in the LC System....................................23
3.4.4 How to Use STOP IN and STOP OUT ...........................................................26
3.5 Tubing ...................................................................................................................27
3.5.1 Tubing of the PU-4086-Binary........................................................................28
3.5.2 Tubing of the PU-4185/4285-Binary...............................................................29
3.5.3 Installing the Tubing Holder and Fixing the Inlet Tubing.................................29
3.5.4 Waste Solvents in the LC System..................................................................31
3.6 Note When Connecting the Tubing .....................................................................33
3.7 Transporting the Pump ........................................................................................34
4. Operations .........................................................................................................35
4.1 Power ON and Self-diagnostic Test.....................................................................35
4.2 Monitor Screen......................................................................................................35
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 4.2.1 Monitor Screen ..............................................................................................35
4.2.2 Monitor Screens while Time Program is Executed .........................................36
4.2.3 Monitor Screen in the LC-Net Connection......................................................37
4.2.4 Monitor Screen Sleeping Function .................................................................37
4.3 Setting and Displaying the Parameters...............................................................37
4.3.1 Setting Compositions (HPG RATIO) ..............................................................40
4.3.2 Setting Flow Rate (FLOW RATE) ..................................................................41
4.3.3 Setting Maximum and Minimum Pressures (MAX/MIN)..................................42
4.3.4 Displaying Operating Pressure without Autozero Compensation
(PRESS MONITOR) ......................................................................................43
4.3.5 Setting Mixing Speed for the Mixing Unit (MIXING SPEED)...........................43
4.3.6 Setting Ranges for Each Pump......................................................................44
4.3.7 Setting Flow Rate Using the [DIRECT] Key ...................................................45
4.4 Preparation of Mobile Phase Solvents ................................................................46
4.4.1 Solvent Filtration ............................................................................................46
4.4.2 General Considerations for Solvent Replacement..........................................47
4.5 Power OFF.............................................................................................................48
4.5.1 Turning the Main Power OFF.........................................................................48
4.6 Leaving the Instrument Idle for Extended Periods .............................................48
5. Operations in Time Program Mode..................................................................49
5.1 Outline of Operations in Time Program Mode ....................................................49
5.2 Time Program Setting Screens............................................................................50
5.3 Setting Program File Number ..............................................................................51
5.4 Setting the Initial Conditions ...............................................................................51
5.5 Setting Time Program...........................................................................................51
5.5.1 Editing Steps .................................................................................................51
5.5.2 Changing Step...............................................................................................53
5.5.3 Inserting and Deleting Step............................................................................53
5.6 Other Settings .......................................................................................................55
5.6.1 Deleting Time Program..................................................................................55
5.6.2 Copying Time Program..................................................................................55
5.7 Time Program Operations ....................................................................................56
5.7.1 Starting Initial Conditions ...............................................................................56
5.7.2 Executing Time Program ...............................................................................56
5.7.3 Stopping Time Program.................................................................................56
5.7.4 Temporarily Parameter Changes while the Time Program is Running ...........56
6. Setting Instrument Parameters ........................................................................57
6.1 Commands ............................................................................................................57
6.1.1 Executing Purging (Solvent Replacement Function) (SOLVENT
PURGE) ........................................................................................................58
6.1.2 Setting Instrument Power OFF Timer (POWER OFF TIMER) ........................59
6.1.3 Setting the Pump ON Timer (PUMP ON TIMER) ...........................................60
6.1.4 Setting the Pump OFF Timer (PUMP OFF TIMER)........................................60
6.2 Instrument Parameters (MENU) ...........................................................................60
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 6.3 Configuration Items (CONFIGURATION) .............................................................62
6.3.1 Setting Pressure Unit (PRESS UNIT) ............................................................62
6.4 Accessory Items (ACCESSORY)..........................................................................63
6.4.1 Confirming/setting the Information of the Mixing Unit (MIXING UNIT) ............63
6.4.2 Confirming/setting the Information of the Degassing Unit (DG#1 UNIT)
65
6.5 Maintenance Items (MAINTENANCE) ..................................................................66
6.5.1 Instrument History (HISTORY).......................................................................66
6.5.2 Setting Instrument Product Parameters (PRODUCT PARAM) .......................67
6.5.3 Setting Error-related Parameters (ERROR SETTING) ...................................68
6.6 TOOL Items (TOOL) ..............................................................................................69
6.6.1 Assigning [Drct] Key Function (DIRECT KEY) ...............................................70
6.6.2 Setting Display Mode (DISPLAY MODE) .......................................................70
[Link] Setting Backlight (BACKLIGHT)..............................................................70
[Link] Setting Automatic Monitor Screen Switching (SWITCH) .........................71
6.6.3 Setting Error Sound (ERROR BEEP).............................................................71
6.6.4 Setting SF-NAV MODE (SF-NAV MODE) ......................................................71
6.6.5 Setting Event Signal Output Unit (EVENT UNIT) ...........................................71
6.7 Information Items (INFORMATION) .....................................................................72
6.7.1 Confirming the Instrument Name (MODEL) ...................................................73
6.7.2 Confirming the Firmware Version/Type (VERSION/TYPE) ............................73
6.7.3 Confirming and Setting Instrument Serial Number (SERIAL NUMBER)
73
6.7.4 Confirming and Setting Pump Number (PUMP NUMBER).............................73
7. Error/Information Messages and Troubleshooting ........................................74
7.1 Error during Self-Diagnosis Test after Power Up ...............................................74
7.1.1 Self-diagnosis Test Items...............................................................................74
7.1.2 BATTERY IS DEAD.......................................................................................74
7.1.3 BACKUP ERROR..........................................................................................74
7.1.4 NVRAM CORRUPTED ..................................................................................75
7.1.5 SET PARAMETERS ......................................................................................75
7.1.6 CAN’T RESET PRESS SENSOR ..................................................................76
7.2 Errors during Operation .......................................................................................76
7.2.1 SOLVENT LEAK............................................................................................76
7.2.2 Errors due to High Pressure (OVER PRESS) ................................................77
7.2.3 Errors due to Exceedingly Low Pressure (UNDER PRESS) ..........................77
7.2.4 ALARM / DG#1 PUMP DOWN.......................................................................78
7.3 Troubleshooting ...................................................................................................78
7.3.1 Abnormal Operating Pressure........................................................................78
7.3.2 When the Operating Pressure is Higher than Normal Pressure .....................80
7.3.3 When the Operating Pressure is Lower than Normal Pressure ......................81
7.3.4 Large Variation in Operating Pressure ...........................................................82
7.4 Baseline Problems................................................................................................83
7.4.1 High Baseline Noise ......................................................................................83
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 7.4.2 Large Baseline Drift .......................................................................................84
7.4.3 Periodic Baseline Drift ...................................................................................85
7.4.4 Appearance of Ghost Peaks ..........................................................................85
7.5 Chromatogram Problems .....................................................................................86
7.5.1 Lengthening Retention Time..........................................................................86
7.5.2 Shortening Retention Time ............................................................................86
7.5.3 Poor Reproducibility in Retention Time ..........................................................86
7.5.4 Poor Reproducibility for Peak Area or Peak Height (Reproducibility for
Retention Time is Good)................................................................................87
7.5.5 Peak Waveform Problems (Peak Broadening, Peak Splitting) .......................87
7.6 Checking Solvent Leaks and Temporary Treatments for the
PU-4086-Binary .....................................................................................................87
7.6.1 Places where Solvent Leaks may Occur for the PU-4086-Binary...................87
7.6.2 Solvent Leaks between the Pump Heads and Head Mound Flanges or
from the Plunger Washing Mechanism Sections for the
PU-4086-Binary .............................................................................................88
7.6.3 Check Valve Assembly Leaks for the PU-4086-Binary...................................89
7.6.4 Purge Valve Leaks for the PU-4086-Binary....................................................90
7.6.5 Outlet Port (Line Filter) Leaks for the PU-4086-Binary ...................................91
7.6.6 Inlet Tubing Fitting Leaks for the PU-4086-Binary..........................................92
7.7 Checking Solvent Leaks and Temporary Treatments for the
PU-4185-Binary .....................................................................................................93
7.7.1 Places where Solvent Leaks may Occur for the PU-4185-Binary...................93
7.7.2 Solvent Leaks between the Pump Heads and Head Mound Flanges or
from the Plunger Washing Mechanism Sections for the
PU-4185-Binary .............................................................................................94
7.7.3 Check Valve Assembly Leaks for the PU-4185-Binary...................................95
7.7.4 Purge Valve Leaks for the PU-4185-Binary....................................................96
7.7.5 Inlet Tubing Fitting Leaks for the PU-4185-Binary..........................................97
7.8 Checking Solvent Leaks and Temporary Treatments for the
PU-4285-Binary .....................................................................................................97
7.8.1 Places where Solvent Leaks may Occur for the PU-4285-Binary...................97
7.8.2 Solvent Leaks between the Pump Heads and Head Mound Flanges or
from the Plunger Washing Mechanism Sections for the
PU-4285-Binary .............................................................................................98
7.8.3 Check Valve Assembly Leaks for the PU-4285-Binary...................................99
7.8.4 Purge Valve Leaks for the PU-4285-Binary..................................................100
7.8.5 Inlet Tubing Fitting Leaks for the PU-4285-Binary........................................101
7.9 Leaving the Instrument Idle for Extended Periods ...........................................101
8. Maintenance ....................................................................................................102
8.1 Regular Maintenance..........................................................................................102
8.2 Air Bubble Removal............................................................................................102
8.3 Solvent Replacement..........................................................................................103
8.3.1 General Considerations for Solvent Replacement........................................103
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 8.3.2 Solvent Replacement...................................................................................104
8.4 Cleaning inside the Pump ..................................................................................105
8.5 Replacing Plunger Seal for the PU-4086-Binary ...............................................105
8.6 Replacing Check Valve Assembly for the PU-4086-Binary ..............................111
8.7 Replacing Purge Valve Needle and Purge Valve Packing for the
PU-4086-Binary ...................................................................................................114
8.8 Replacing Line Filter for the PU-4086-Binary....................................................118
8.9 Replacing Magnetic Stirrer and Filter in the Mixing Chamber for the
PU-4086-Binary ...................................................................................................119
8.10 Replacing Plunger Seal for the PU-4185-Binary ...............................................124
8.11 Replacing Check Valve Assembly for the PU-4185-Binary ..............................131
8.12 Replacing Purge Valve Needle and Purge Valve Packing for the
PU-4185-Binary ...................................................................................................134
8.13 Replacing Plunger Seal for the PU-4285-Binary ...............................................138
8.14 Replacing Check Valve Assembly for the PU-4285-Binary ..............................144
8.15 Replacing Purge Valve Needle and Purge Valve Packing for the
PU-4285-Binary ...................................................................................................148
8.16 Replacing Magnetic Stirrer and Filter in the Mixing Chamber for the
PU-4185/4285-Binary ..........................................................................................152
8.17 Replacing the Power Fuses ...............................................................................157
9. Optional and Replacement Parts ...................................................................158
9.1 Standard Accessories ........................................................................................158
9.2 Start-up Kits and Maintenance Tool Kit ............................................................159
10. Consumable Parts...........................................................................................164
10.1 Consumable Parts for the Inlet Tubing .............................................................164
10.2 Consumable Parts for the PU-4086-Binary .......................................................165
10.2.1 Consumable Parts of the Pump Head Section for the PU-4086-Binary ........165
10.2.2 Consumable Parts of the Outlet Port Section for the PU-4086-Binary..........167
10.3 Consumable Parts for the PU-4185Binary ........................................................168
10.3.1 Consumable Parts of the Pump Head Section for the PU-4185-Binary ........168
10.4 Consumable Parts for the PU-4285-Binary .......................................................170
10.4.1 Consumable Parts of the Pump Head Section for the PU-4285-Binary ........170
10.5 Optional Cables for the Pumps..........................................................................172
11. Options for the Mixing Unit ............................................................................173

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xviii
1. Overview and Specifications
1.1 Overview
The models PU-4086-Binary/4185-Binary/4285-Binary are high performance, high
functionality high-pressure mixing gradient pumps for High-performance Liquid
Chromatography (HPLC), Rapid Separation Liquid Chromatography (RHPLC) and/or Ultra
High-performance Liquid Chromatography (UHPLC).
These pumps offer a one-solvent constant flow delivery and continuous solvent delivery of
two-solvent mixing at a specified mixing ratio.
In addition, user-defined time program adjusts gradient composition, flow rate, and maximum
or minimum pressure by using time program.
Other functions are as follows.
1) Automatic pump shut-off and pump on timer.
2) Automatic instrument power off timer.
3) Maintenance functions such as self-diagnostics and plunger washing.

1.2 Specifications
(1) Specifications for PU-4086-Bianry

Pumping mode: Fixed flow rate

For constant flow rate analysis:

Flow rate setting range: 0.001 to 20.0 mL/min (0.001 mL/min increments)
Applicable flow rate range: 0.5 to 20.0 mL/min
Flow rate accuracy: ± 1 % of setting value or ± 10 µL/min, whichever is larger
(a flow rate range of 0.5 to 20.0 mL/min is assumed.)
Flow rate precision: 0.05 % RSD (Relative Standard Deviation) or ± 0.04 min
SD (Standard Deviation), which is larger
(a flow rate range of 0.5 to 20.0 mL/min is assumed. Under
specified conditions. Measurement by chromatogram)
Maximum pressure: 50.0 MPa
Pressure display accuracy: ± 5 % of setting value or ± 1 MPa, whichever is larger
(Average pressure value under a constant loading)
Maximum pressure limit setting: 0.0 to 50.0 MPa (0.1 MPa increments)
Minimum pressure limit setting: 0.0 to 50.0 MPa (0.1 MPa increments)
Mixing accuracy: ± 0.75 %
(a composition ratio from 5 to 95 %, flow rate range: 0.5 to
20.0 mL/min)
Mixing precision: 0.2 % RSD, or ± 0.03 min SD, which is larger
(a flow rate range of 0.5 to 20.0 mL/min is assumed. Under
specified conditions. Measurement by chromatogram)

1
Standard mixing chamber volume: 5 mL
Wetted materials: Sapphire, ruby, zirconia ceramics, stainless steel
(SUS316), PTFE, and PEEK
Applicable pH range: 1.0 to 12.5

Program functions:
Time program: Programming of flow rate, composition, maximum
pressure, minimum pressure
High-pressure gradient: 2-solvent high-pressure (mixing) gradient using a
PU-4086-Binary.
(also can be control by a chromatography control system
(i.e. ChromNAV Ver.2)
Composition ratio setting: 0 to 100 % (0.1 % increments)
Timer: Pump ON/OFF timer, maximum setting: 99.9 hours
(0.1-hour increments)
Instrument power off timer, maximum setting: 99.9 hours
(0.1-hour increments)
Display and settings: 16-character, 2-line LCD multi-display; settings entered
using function keys
Pressure sensor: Flow-through strain gauge transducer

External control functions:

Event relay input: Pumping start, pumping stop, and program reset/run
Specialized control
input and output: LC-Net®
General control input and output: RS-232C (Optional)

Diagnostic functions:
Items checked: Memory (ROM, RAM), pressure sensors, and memory
backup
Maintenance program: Pressure sensor inspection functions

Usable temperature range: 10 °C to 30 °C

Additional specifications:
Dimensions: 300(W) x 470(D) x 150(H) mm (except protruding portion),
approximate weight: 21.0 kg
Required power: AC 100 to 240 V, 50/60 Hz, maximum 100 VA

2
(2) Specifications for PU-4185-Binary

Pumping mode: Fixed flow rate

For constant flow rate analysis:

Flow rate setting range: 0.001 to 4.0 mL/min (0.001 mL/min increments)
Applicable flow rate range: 0.050 to 3.0 mL/min (70MPa or less)
3.001 to 4.0 mL/min (40 MPa or less)
Flow rate accuracy: ± 1 % of setting value or ± 2 µL/min, whichever is larger
(a flow rate range of 0.05 to 4.0 mL/min is assumed.)
Flow rate precision: 0.05 % RSD or ± 0.04 min SD, which is larger
(a flow rate range of 0.1 to 4.0 mL/min is assumed. Under
specified conditions. Measurement by chromatogram)
Maximum pressure: 70.0 MPa (3.0 mL/min or less)
40.0 MPa (4.0 mL/min or less)
Pressure display accuracy: ± 5 % of setting value or ± 1 MPa, whichever is larger
(Average pressure value under a constant loading)
Maximum pressure limit setting: 0.0 to 70.0 MPa (0.1 MPa increments)
Minimum pressure limit setting: 0.0 to 70.0 MPa (0.1 MPa increments)
Mixing accuracy: ± 0.40 %
(a composition ratio from 5 to 95 %, flow rate range: 0.2 to
4.0 mL/min)
Mixing precision: 0.15 % RSD, or ± 0.01 min SD, which is larger
(a flow rate range of 0.2 to 4.0 mL/min is assumed. Under
specified conditions. Measurement by chromatogram)
Standard mixing chamber volume: 400 µL
Wetted materials: Sapphire, ruby, zirconia ceramics, stainless steel
(SUS316), PTFE, and PEEK
Applicable pH range: 1.0 to 12.5
Degassing unit: Equipped as standard
Number of channels: 4 (can expand to 5 channels as
optional)
Maximum usable flow rate: 5 mL/min
Volumes per channel: approx. 400 µL
Wetted materials: special fluoride resin (Teflon AF), PPS,
PEEK, and PTFE
High-pressure gradient: 2-solvent high-pressure (mixing) gradient using a
PU-4185-Binary (stand alone)
(also can be control by a chromatography control system
(i.e. ChromNAV Ver.2)
Composition ratio setting: 0 to 100 % (0.1 % increments)

3
Program functions:
Time program: Programming of flow rate, composition, maximum
pressure, minimum pressure
Timer: Pump ON/OFF timer, maximum setting: 99.9 hours
(0.1-hour increments)
Instrument power off timer, maximum setting: 99.9 hours
(0.1-hour increments)
Display and settings: 16-character, 2-line LCD multi-display; settings entered
using function keys
Pressure sensor: Flow-through strain gauge transducer

External control functions:

Event relay input: Pumping start, pumping stop, and program reset/run
Specialized control
input and output: LC-Net®
General control input and output: RS-232C (Optional)

Diagnostic functions:
Items checked: Memory (ROM, RAM), pressure sensors, and memory
backup
Maintenance program: Pressure sensor inspection functions

Usable temperature range: 10 °C to 30 °C

Additional specifications:
Dimensions: 300(W) x 470(D) x 150(H) mm (except protruding portion),
approximate weight: 20.5kg
Required power: AC 100 to 240 V, 50/60 Hz, maximum 130 VA

(3) Specifications for PU-4285-Binary

Pumping mode: Fixed flow rate

For constant flow rate analysis:

Flow rate setting range: 0.001 to 2.0 mL/min (0.001 mL/min increments)
Applicable flow rate range: 0.050 to 1.5 mL/min (130 MPa or less)
1.501 to 2.0 mL/min (100 MPa or less)
Flow rate accuracy: ± 1 % of setting value or ± 2 µL/min, whichever is larger
(a flow rate range of 0.05 to 2.0 mL/min is assumed.)
Flow rate precision: 0.05 % RSD or ± 0.04 min SD, which is larger
(a flow rate range of 0.1 to 2.0 mL/min is assumed. Under
specified conditions. Measurement by chromatogram)

4
Maximum pressure: 130.0 MPa (1.5 mL/min or less)
100.0 MPa (2.0 mL/min or less)
Pressure display accuracy: ± 5 % of setting value or ± 1 MPa, whichever is larger
(Average pressure value under a constant loading)
Maximum pressure limit setting: 0.0 to 130.0 MPa (0.1 MPa increments)
Minimum pressure limit setting: 0.0 to 130.0 MPa (0.1 MPa increments)
Mixing accuracy: ± 0.40 %
(a composition ratio from 5 to 95 %, flow rate range: 0.2 to
2.0 mL/min)
Mixing precision: 0.15 % RSD, or ± 0.01 min SD, which is larger
(a flow rate range of 0.2 to 2.0 mL/min is assumed. Under
specified conditions. Measurement by chromatogram)
Standard mixing chamber volume: 250 µL
Wetted materials: Sapphire, ruby, zirconia ceramics, stainless steel
(SUS316), PTFE, and PEEK
Applicable pH range: 1.0 to 12.5
Degassing unit: Equipped as standard
Number of channels: 4 (can expand to 5 channels as
optional)
Maximum usable flow rate: 5 mL/min
Volumes per channel: approx. 400 µL
Wetted materials: special fluoride resin (Teflon AF), PPS,
PEEK, and PTFE
High-pressure gradient: 2-solvent high-pressure (mixing) gradient using a
PU-4285-Binary (stand alone)
(also can be control by a chromatography control system
(i.e. ChromNAV Ver.2)
Composition ratio setting: 0 to 100 % (0.1 % increments)

Program functions:
Time program: Programming of flow rate, composition, maximum
pressure, minimum pressure
Timer: Pump ON/OFF timer, maximum setting: 99.9 hours
(0.1-hour increments)
Instrument power off timer, maximum setting: 99.9 hours
(0.1-hour increments)
Display and settings: 16-character, 2-line LCD multi-display; settings entered
using function keys
Pressure sensor: Flow-through strain gauge transducer

External control functions:

Event relay input: Pumping start, pumping stop, and program reset/run
Specialized control

5
 input and output: LC-Net®
General control input and output: RS-232C (Optional)

Diagnostic functions:
Items checked: Memory (ROM, RAM), pressure sensors, and memory
backup
Maintenance program: Pressure sensor inspection functions

Usable temperature range: 10 °C to 30 °C

Additional specifications:
Dimensions: 300(W) x 470(D) x 150(H) mm (except protruding portion),
approximate weight: 22.5 kg
Required power: AC 100 to 240 V, 50/60 Hz, maximum 110 VA

6
2. Parts and Functions
2.1 Exterior

(4)

(1) (5)

(2)

(3)

(5) (6)

(4)
(7)

Figure 2.1 Exterior (Close the Front Doors)

No. Name Function

Contains the pump control and parameter editing keys.
1 Operation Panel
See Section 2.2 for details.
Allows access to internal components necessary when
2 Front Door
operating the purge valve or replacing solvents.
Manual Injector Fixing Fixing holes for attaching the manual injector (with
3
Holes L-shape panel) (option).

7
 Collects dew condensation water or leak solvents from
the instrument stacked upon the pump to the Drain Port
(Top), and introduces to the Drain Port (Bottom) passing
Drain Ports through the drain tubing placed in the left side of the
4
(Top) (Bottom) pump.
Also collects the leak solvents from the parts of the
pump to the Drain Port (Bottom).
See Section 3.3.1 for details.
Aseismatic Kit Fixing holes for attaching the connection fittings of the
5
Fixing Holes optional Aseismatic Kit.
Fixing holes for attaching the Tubing Holder, included in
Tubing Holder
6 the standard accessories.
Fixing Holes
See Section 3.5.3 for details.
Fix internal moving parts (i.e. pump driving section) to
prevent damage during shipping.
Fixing Holes for
7 The fixing places depend on the combination of the
Shipping
pumps in the box.
See Section 3.1 for details.

2.2 Operation Panel

Power Button Power LED LCD Screen

Figure 2.2 Operation Panel and Key Section

8
 Name Function
The LED is red and the power button is active (standby condition)
when the main power switch (see Section 2.5) is on. If the power
Power Button, LED button is pressed in this condition, the LED is turned blue and the
instrument starts running. If the power button is pressed again, the
instrument shuts down and the LED is again red.
Operating conditions, settings, and error messages are displayed on
the screen.
While editing the parameters, Up, Down, Right, and Left key
LCD Screen operation guide arrows (▲▼◄►) are displayed on the rightmost
line. When some of these arrows are displayed, pressing the
displayed arrow key moves to the next screen such as the parameter
setting screen.
Used to run or stop the time program.
Every press of this key changes the time program execution
condition.
[Run/Stop] Key, Time program stops (LED is off.)
LED Time program initial condition running (LED blinks green.)
Time program is executing (LED is green.)
Also, the LED lamp is turned on red when an error occurs, and
orange when a warning item occurs.
[Clear] Key Used to clear parameter input. Also used to cancel errors.
Press this key to start the pump flow (LED lamp is lit). Press again to
[Pump] Key, LED
stop the pump flow (LED lamp is lit off).
Pressing this key can directly move to the assigned parameter setting
[Drct] Key, LED screen. The parameter setting screen which can be assigned to the
[Drct] key is shown in Section 6.6.1.

9
The keys described in the following table have different functions while the monitor screen is
displayed and while the parameter is editing.

Functions
Name While selecting items selecting or
While monitor screen is displayed
editing parameters
Moves to the instrument
Used to select, change or enter
[Menu/Enter] key parameter setting screen (see
the editing item.
Section 6.2).
Used to change the editing
Displays, sets, or executes the
[Cmd/▲] key screen or to change the setting
command item (see Section 6.1).
value.
Used to change the editing
Moves to the time program screen or to change the setting
[Prgm/◄] key
editing screen (see Chapter 5). value. For numerical entry,
moves the editing digit.
Moves to the measurement Used to change the editing
[Param/▼] key parameter setting screen screen or to change the setting
(see Section 4.3). value.
Used to change the editing
screen or to change the setting
[Disp/►] key Changes the monitor screens.
value. For numerical entry,
moves the editing digit.

Note: In this manual, the keys, [Cmd/▲], [Prgm/◄], [Param/▼], and [Disp/►] are shown as
[ ▲ ], [◄], [ ▼ ], and [►], respectively, for easy descriptions when explaining the
operations for the item selection and editing.

10
2.3 Inside of the PU-4086-Binary

(1)
(3)
(5)

(1)

(2)
(4)

(6) (2)

Figure 2.3 Inside the PU-4086-Binary (Opening the Front Door)

No. Name Function

Solvent elutes from the drain tubing when the purge
Drain Tubing
valve is turned counterclockwise. When replacing
1 and
solvents and removing air from the pump heads,
Purge Valve
operate this valve.
2 Pump Heads Solvents are delivered from two delivery heads.
Mixing chamber for mixing two solvents delivering from
right and left pump units. The solvents are mixed by the
3 Mixing Chamber magnetic stirrer placed in the chamber. Two solvents
are entered from the side ports, and mixed solvent goes
out from the front port.
Plunger washing solvent is drawn in and subsequently
Washing Solvent discharged through these ports.
4
Inlet/Outlet Ports are on each head mount flange behind each pump
head.

11
 When solvents leak from the pump head section, the
5 Leak Solvent Tray
solvents are accepted in this tray.
Detects solvent leaks. The sensor is placed underneath
6 Leak Sensors
each pump unit.

2.4 Inside of the PU-4185/4285-Binary

(3)
(7)

(2)
(1) (4)
(6)
(6) (2)
(5) (1) (4)
(6)

Figure 2.4 Inside of the PU-4185/4285-Binary (Opening the Front Door)

No. Name Function

Solvent elutes from the drain tubing when the purge
Drain Tubing
valve is turned counterclockwise. When replacing
1 and
solvents and removing air from the pump heads,
Purge Valve
operate this valve.
When you face the instrument, the delivery head is on
2 Pump Heads the left, with the inlet and outlet check valves for solvent
delivery. The modifier head is on the right.
Mixing chamber for mixing two solvents delivering from
3 Mixing Chamber right and left pump units. The solvents are mixed by the
magnetic stirrer placed in the chamber. Two solvents

12
 are entered from the side ports, and mixed solvent goes
out from the front port.
Plunger washing solvent is drawn in and subsequently
Washing Solvent discharged through these ports.
4
Inlet/Outlet Ports are on each head mount flange behind each pump
head.
When solvents leak from the pump head section, the
5 Leak Solvent Tray
solvents are accepted in this tray.
Detects solvent leaks. The sensor is placed underneath
6 Leak Sensors
each pump unit.
7 Degassing Unit 4-channel inline degasser.

13
2.5 Rear View
(4) (5)

(6)

(1)

(2)

(3)
(7) (8) (9)

Figure 2.5 Rear Panel

No. Name Function

Instrument main power switch.
When this switch is turned on, the power button LED on
the operation panel is turned on red and the instrument
1 Main Power Switch
starting can be done.
When it is turned off, all power supplied to the
instrument is cut off.
2 “AC INPUT” Accepts the power cable.
Accepts the power fuses.
3 Power Fuses
See Section 8.17 for replacing the power fuses.
Input/Output Terminals for receiving or sending the
4 Input/Output Terminals analog signals from/to external instruments through
these terminals.
Accepts LC-Net cable(s). Used to control this instrument
“LC-Net” Connectors
5 with the JASCO Chromatography Data System such as
(2 circuits)
ChromNAV Ver.2.
Optional Terminal Accepts optional terminal.
6
Fixing Holes
7 “Exhaust” Port Accepts the in-line degasser exhaust port.
Connection for grounding the instrument.
Ground Terminal
8 Note: Do not use this as the GND terminal for the
(GND) input/output signals.

9 Cooling Fan Cooling fan for the instrument.

14
2.6 Input/Output Terminals

Figure 2.6 Input/Output Terminals

Name Function
Input terminals

RESET/RUN When a contact closure signal (close) is input from an external

instrument, the time program starts running.
When it is input while the time program is executed, the current
program stops immediately, and the time program restarts from the
initial conditions.
PUMP ON When a contact closure signal (close) is input from an external
instrument, the pump starts flowing.
PUMP OFF When a contact closure signal (close) is input from an external
instrument, the pump stops flowing.
Output terminals

STOP OUT The contact is open normally. A contact closure signal (close) is
output if an error occurs in the instrument.
PRESS SIGNAL Outputs an analog signal proportional to the pressure.
The scale is 20 mV/10.0 MPa for the PU-4086-Binary and
PU-4185-Binary, and 10 mV/10 MPa for the PU-4285-Binary.
OPTION Currently not used (terminal for optional).

15
3. Installation

WARNING!
When installing the instrument, obey the installation procedure and precaution to prevent
unexpected injury.

3.1 Unpacking and Inspection

(1) Open the box and take out the instrument (see Figure 3.1).

Note: The pump weighs approximately 21.0 kg for the PU-4086-Binary, 20.5 kg for the
PU-4185-Binary, and 22.5 kg for the PU-4285-Binary. When taking out the
instrument from the box, be sure to hold the bottom plate shown in Figure 3.1.

Figure 3.1 Holding Positions for Carrying

CAUTION
The front door is made by resin and is not designed to carry weight. If you carry the
instrument with putting your hands on the bottom of the front door, the door may be broken.
Always hold the instrument bottom as shown in Figure 3.1 to take out the instrument from
the box.

(2) Remove the protection cushions between the front door and the chassis after removing
the fixing tapes (see Figure 3.2).
16
 Protection Cushion

Figure 3.2 Removing the Protection Cushions

(3) Next, remove six black holding screws (on the bottom panel) securing the internal moving
parts of the pump (see Figure 3.3) using a Phillips screwdriver.
(Do not remove other color screws.)

Tools required: Phillips screwdriver

Figure 3.3 Removing the Holding Screws (on the Bottom Panel)

17
(4) Make sure the serial number on the rear panel matches the serial number on Certificate
of Inspection. Also, make sure the required power described in the seal is correct.

PU-4x8x-Binary

Figure 3.4 Serial Number

(5) Check the instrument for damage. If damage is found, contact your local JASCO
distributor.
Remove the accessories and make sure that each part on the detailed list of standard
accessories is present (The list is in the accessory box). If a part is missing or damaged
or if there is any problem, contact your local JASCO distributor.

3.2 Installation
High-performance Liquid Chromatogram uses large amount of solvents. So, make sure the
installation site meets the environmental specifications considering that solvents may be
over-flowed or evaporated.
When installing the system, obey the following Warning! and Cautions. Also see the
Installation Requirement in the preface section.

WARNING!
(1) Install the instruments in order not to fall down by vibration, an earthquake, etc. If the
instruments fall down, accident or injury may occur.
(Optional Aseismatic Kit is available for the LC-4000 series system.)
(2) Install the instruments to a place where there are ventilation facilities or devices (e.g.
drafter) to prevent filling the room with solvent vapor. Also, control waste solvents by a
suitable method.

18
 CAUTION
(1) The installation bench should have a flat level surface. The installation bench should
be sufficiently deep in order to allow an additional 10 cm or more of space at the rear
that can withstand the weight of the instruments.
Also, arrange the instruments in a place without vibration. If there is vibration, the
instruments may fall causing accident or injury. Furthermore, performance may be
hindered when there is vibration.
(2) Be sure not to put your finger in the spaces between the instruments when installing.
(3) When arranging the instruments, be sure to hold the instrument correctly (See the
operator’s manual of the instrument).
(4) When arranging the instruments, due to heat dissipation, leave 10 cm or more
between the wall and the back of each instrument.

3.3 Installing the Pump to LC System

The pump constructs an LC system with autosampler, detector, column oven, etc.
The instrument configuration of the LC system depends on the application. A small system
can be stacked, but a large system should be two-stacked to avoid excessive height of the
stack. Also, consider the system volume minimized. See Section 3.3.2 for the examples of
system arrangement.
Before constructing the system, attach the Drain Port (Bottom) or the End Drain Port to the
waste solvent flow line on the pump (see Section 3.3.1) (They are included in the standard
accessories). When the pump is installed at the bottom of the stack, attach the End Drain Port,
and when it is installed at the top or middle of the stack, attach the Drain Port (Bottom). See
Section 3.3.1 for fixing the port.

3.3.1 Attaching Drain Port (Bottom) or End Drain Port

Before constraucting the LC system, attach the Drain Port (Bottom) or End Drain Port, which
is included in the standard accessories, to the bottom of the right front of the pump using two
of M2 pan-head screws included in the accessories (see Figure 3.5).
When the pump is installed at the bottom of the stack, attach the End Drain Port so that the
waste port is positioned right. The waste tubing route to a waste bottle is connected to the End
Drain Port.
When the pump is installed at the top or middle of the stack, attach the Drain Port (Bottom).

19
 Supplied Screws
(M2)
Drain Port (Bottom) End Drain Port

Figure 3.5 Attaching the Drain Port (Bottom) or End Drain Port

3.3.2 LC System Examples

Two arrangement examples of the LC system, which consists of the PU-4185-Bomary pump,
AS-4150 autosampler, UV-4070 UV/VIS detector, CO-4061 column oven, and BS-4000-1
bottle stand, are shown in Figure 3.6 and Figure 3.7.
Prepare the installation bench which is sufficiently deep in order to allow an additional space
at the rear that can withstand the weight of the instruments.

20
 Bottle Stand

UV/VIS Detector

Column Oven

Autosampler

Pump

Figure 3.6 Example of Stacked System

Bottle Stand
UV/VIS Detector

Column Oven
Autosampler

Pump

Figure 3.7 Example of Two-stacked System

21
3.4 Wiring
3.4.1 Connecting Power Cable
The power supply voltage for this instrument is AC 100 V to AC 240 V.
Use an appropriate power cable according to the local voltage requirements such as 100 to
120 Volts system and 220 to 240 Volts system. Also use an appropriate rating of fuses to the
source voltage (100 to 120 V: T2.5A, 220 to 240 V: T1.6A).
Be sure to use a power supply with sufficient capacity. If the instrument does not receive a
sufficient voltage, a power failure or a drop in electric power may occur. In this case, not only
the pump but also other instruments that are connected to the same power supply may be
adversely affected.

WARNING!
(1) Use the power cable carefully. Do not force, stretch or to bend the cable. If the power
cable is broken, replace with a new one.
(2) Be sure that the instrument is grounded reliably.
If the instrument is not grounded, there is a risk of an electric shock. Do not use water
piping for grounding purposes since these pipes often use non-metallic materials (vinyl
chloride). Also, do not use gas pipes to ground the instrument for safety reasons. Use
the ground terminals of the wall socket.

CAUTION
(1) Check that the pump power is turned off when inserting the power cable in the inlet
terminal.
(2) Ensure that the instrument is grounded properly for stable operation of the instrument,
to protect the instrument from electrical noise, and to avoid trouble or an electric
shock. Use a three-pronged electrical outlet with a ground. When only a two-pronged
socket is available, use a 2p-3p adapter and be sure to ground the ground wire of the
adapter.

3.4.2 Connecting LC-Net Cable in the LC System

When controlling the instruments including the pump with the Chromatography Data System
(e.g. ChromNAV Ver.2), connect the LC-Net cables. Use the AG Cable for the connection
between the controller (LC-Net II/ADC) and the instrument, and CG Cable for the connection
between the instruments. Figure 3.8 shows an example.

Note: There are two types of LC-Net cables. Be sure that these cables are connected
correctly as shown in the following table.

22
 Name Part Number Connection
AG Cable 6688-H563A between the controller (LC-Net II/ADC) and
the instrument
CG Cable 6688-H564A between the instruments

Autosampler LC-Net II/ADC

AG Cable

CG Cable

Pump

Figure 3.8 LC-Net Cable Connection in the LC-system

3.4.3 Interlocking with Other Instruments in the LC System

When the autosampler is controlled with the Chromatography Data System such as
ChromNAV, connect the dedicated cable, which is included in the LC-Net II/ADC, between the
“INJ. MARKER” terminals on the autosampler and the “REMOTE INJECT” terminals on the
LC-Net II/ADC as shown in Figure 3.10. If the PDA detector is included in the system, connect
another cable between the “INJ. MARKER” terminals on the autosampler and the “START IN”
on the PDA detector. The program, such as the measurement conditions of each instrument
connected by the LC-Net Cable in the LC system, and the chromatogram data acquisition can
be started at the time of sample injection by the autosampler, or at the time of sample
introduction into the column (when the injection delay time is set to the autosampler control

23
method of the ChromNAV, at the time of elapsing the set time). The detector initial conditions
set in the ChromNAV control method are run through the LC-Net at the time of autosampler
sample injection.

CAUTION
Connect the INJECTION MARKER signal from one circuit to one instrument. Do not
connect the signal from one circuit to multiple instruments.

Note: When sample injection is executed, a contact closure signal (approximately a

0.6-second pulse) is output from the “INJ. MARKER” terminals. Normally, the
INJECTION MARKER signal status is OPEN.

When connecting the cable to the input/output terminals on the rear panel, insert the cable
terminal bar with pushing the black button above the inserting position by your finger (see
Figure 3.9).

Signal Cable

Figure 3.9 Connecting the Signal Cable

24
 PDA Detector

+ -

LC-Net II/ADC

+ - + -

Autosampler

Pump

Figure 3.10 Interlocking with Chromatography Data System

When the Chromatography Data System is not used, connect the “INJ. MARKER” terminals
on the autosampler with the “Reset/Run” terminals (e.g. “PRGM RS/RN”, “PRG R/S”) of other
LC-4000 series instrument as shown in Figure 3.11. Programmed operations of the
connected instrument can be executed.
For example, when the autosampler injects a sample, the set time program of the pump can
be reset and executed, and the UV/VIS detector can be executed autozero. Contact closure
signal is send to the pump and UV/VIS detector every time the autosampler injects a sample.

25
 + - UV/VIS Detector

+ - + -

Autosampler

+ -
Pump

Figure 3.11 Interlocking with Other Instruments (when the Chromatography Data System is
not Used)

3.4.4 How to Use STOP IN and STOP OUT

The pump incorporates circuits that can send or receive signals to or from external
instruments. When the Chromatography Data System such as ChromNAV Ver.2 is not used,
the operations of the instruments in the LC system can be linked by using these circuits.
Figure 3.12 shows an example wiring set-up between the AS-4150 autosampler and the
PU-4185-Binary pump. When the instruments are wired in this way, the pump will stop in the
event trouble occurs with the autosampler, thereby avoiding solvent loss. Conversely, if the
pump stops for some reason (e.g. OVER PRESS), the autosampler will also stop, preventing
empty operation and sample loss of the autosampler.

26
 (1)

(2)

(2) (1)
+ - + -

Autosampler + - + - Pump

(1) (2)

Figure 3.12 Example of How to Use STOP IN and STOP OUT

(when the Chromatography Data System is not Used)

3.5 Tubing
When various columns, detectors, pumps, and other types of instrument are being used to
create an LC system, several types of tubing and connections are required.
The standard pump includes only the minimum parts required for its operation. To create an
LC system, tubing connecting between the instruments and signal cables are required.
JASCO offers several types of Start-up Kit to create the system. The Start-up Kits for HPLC,
RHPLC/UHPLC, Preparative LC (Prep-LC), and Inert LC are available. The kit includes all the
tubing for creating the system (e.g. the system shown in Figure 3.6 and Figure 3.7) and spare
parts used for the system. Use one of the kits that meet your application. In addition, some
tools are required to connect tubing etc. The Maintenance Tool Kit is available.
See Section 9.2 for details on the Start-up Kits and Maintenance Tool Kit.

27
3.5.1 Tubing of the PU-4086-Binary
Figure 3.13 shows the inlet tubing to the pump and the tubing from the pump outlet.

Tubing to the Pump Outlet

Inlet Tubing Inlet Tubing

Figure 3.13 Tubing inside the PU-4086-Binary

(1) Inlet tubing from the mobile phase solvent bottle

Using the tubing holder on the right side panel and the holder inside the pump, connect
the inlet tubing to the pump inlet port passing through the spaces between the right side,
left side, or bottom and the front panel. See Section 3.5.3 for installing the tubing holder
onto the left side panel of the pump.

(2) Outlet tubing to the pump outlet port

Connect an outlet tubing from the pump outlet port to the mixing unit, manual injector inlet,
or autosampler inlet. Use 1/16-inch OD of stainless steel tube or PEEK tube for the
connection. If operating pressure is relatively high, use a stainless steel tube.

28
3.5.2 Tubing of the PU-4185/4285-Binary
Figure 3.14 shows the inlet tubing to the pump and the tubing from the pump outlet.

Tubing to the Pump Outlet

Inlet Tubing Inlet Tubing

Figure 3.14 Tubing inside the PU-4185/4285-Binary

(1) Inlet tubing from the mobile phase solvent bottle

Using the tubing holder on the left side panel and the holder inside the pump, connect the
inlet tubing to the inlet ports of the degassing unit passing through the spaces between
the right side, left side, or bottom and the front panel. See Section 3.5.3 for installing the
tubing holder onto the left side panel of the pump.

(2) Outlet tubing to the pump outlet port

Connect an outlet tubing from the outlet port of the mixing unit to a manual injector inlet,
or autosampler inlet. Use 1/16-inch OD of stainless steel tube or PEEK tube for the
connection. If operating pressure is relatively high, use a stainless steel tube.

3.5.3 Installing the Tubing Holder and Fixing the Inlet Tubing
The Tubing Holder for holding or fixing the inlet tubing can be installed on the left side of the
pump. The holder is included in the standard accessories.
The Tubing Holder can hold two 1/16-inch OD inlet tubing at both ends of the holder and five
1/8-inch inlet tubing at the center (see Figure 3.15).

29
 For 1/16-inch OD
Inlet Tubing

Figure 3.15 Tubing Holder

Figure 3.16 shows an example of fixing the inlet tubing to the Tubing Holder.

Inlet Tubing Inlet Tubing

for the Pump for the Autosampler Flushing
(1/8-inch OD) Solvent
(1/16-inch OD)

Figure 3.16 Example of Fixing the Inlet Tubing to the Tubing Holder

30
Install the Tubing Holder to the pump as follows.
(The screws for fixing the Tubing Holder are included in the standard accessories.)

Using a Phillips screwdriver, tighten the two screws to fix the holder to the pump (see Figure
3.17).

Screw Holes for the

Tubing Holder

Tubing Holder

Figure 3.17 Installing the Tubing Holder

3.5.4 Waste Solvents in the LC System

Each LC-4000 series instrument has the waste solvent ports on the right side of the
instrument for introducing dew condensation water and leak solvents from the instrument to a
waste bottle passing through the under-stacked instrument.
The top waste solvent port and bottom waste port are connected by the tubing in the
instrument. The waste solvents are introduced from the bottom waste solvent port (called
Drain Port (Bottom)) to the top waste solvent port (called Drain Port (Top)), and finally
introduced to a waste bottle through the waste tubing connected to the End Drain Port of the
bottom-stacked instrument.
Using this waste solvent line, the solvents generated in the instruments are wasted to outside
the LC system.
Figure 3.18 shows an example.
Install the End Drain Port, which is included in the standard accessories, as the bottom waste
port of the bottom-stacked instrument to connect the waste solvent tubing. See Section 3.3.1
for installing the End Drain Port.

31
 Between the Instruments
Drain Port (Bottom) and Drain Port (Top)

Bottom-stacked Instrument
End Drain Port
(for connecting the waste solvent tubing)

Waste Tubing

Figure 3.18 Waste Solvent Line in the LC System

The mobile phase must not be introduced to the waste line described above. It must be
introduced directly from the outlet tubing of the detector flow cell to the waste bottle using
other tubing (see Figure 3.19).

32
 Mobile Phase Waste
Solvent
(From Outlet Port of
Detector Flow Cell)

Figure 3.19 Wasting the Mobile Phase

3.6 Note When Connecting the Tubing

In RHPLC (Rapid Separation High-performance Liquid Chromatography) or UHPLC (Ultra
High-performance Liquid Chromatography) analysis, peak is detected as very sharp peak
which width is less than 5 seconds. Therefore, peak dispersion caused by the volume in the
system and tubing connection largely affects on the peak shape. Pay attention for the tubing
connection.
For example, if there are spaces between the tubing (see Figure 3.20), which becomes dead
volume, peak dispersion is caused at here and affects on the peak shape. Therefore, connect
the tubing firmly as shown in Figure 3.20 (Left).

33
 Good connection Bad connection

Figure 3.20 Tubing Connection

3.7 Transporting the Pump

Before moving the pump around in the laboratory, move the pump after disconnecting the
tubing and wiring connected with the mixing unit, autosampler, detector, etc.
Also, move the pump by holding the designated places.

CAUTION
If you carry the instrument without putting your hands on the designated places, the door
may be broken.

34
4. Operations
4.1 Power ON and Self-diagnostic Test
While the LED lamp of the power button is lit on red after turning on the main power switch on
the rear panel, press the power button on the operation panel to turn the instrument power on.
The LED lamp is lit on blue.
The model name and firmware program version are displayed, and then run the
self-diagnostic test automatically. The items checked are listed below;

・ Volatile memory
・ Nonvolatile memory
・ Check of pressure zero and execution of autozero

When a problem is detected, ERROR is displayed and the self-diagnostic test stops. Check
the error item displayed in the screen, and press the [Clear] key to resume the self-diagnostic
test.
The monitor screen is displayed when the self-diagnostic test is complete (see Section 4.2.1).

Note: Vampire (standby) energy during the LED of the Power Button is lit on red is less than
1 W/h. Power consumption is not so many even if the main power switch is kept ON.
So, power ON/OFF by the Power Button on the operation panel is at ease for normal
power ON/OFF operation.

4.2 Monitor Screen

4.2.1 Monitor Screen
The monitor screen appears when the initialize is complete (see Figure 4.1).

Set Flow Rate

1.000mL/min [►]
1.000mL/min
0.0MPa L 100.0/ 0.0 R

Current Pressure Current Composition

Figure 4.1 Monitor Screen

35
Table 4.1 explains the terms shown on the monitor screen.

Table 4.1 Monitor Screen Terms and Meanings

Term Meaning
1.000 mL/min Set flow rate (mL/min)
0.0 MPa Current operating pressure is shown.
(This is the value after executing autozero during the initialization.)
The pressure unit is selectable from MPa, bar, psi, and at (kgf/cm2).
See Section 6.3.1 for selecting the pressure unit.
L100.0 / 0.0 R Current composition is shown.
“L 100.0” shows the composition of the pump on the observers' left is
100.0 % and “0.0 R” shows the composition of the pump on the
observers’ right is 0.0 %.
(See Section 4.3.1 for setting the compositions.)

4.2.2 Monitor Screens while Time Program is Executed

When the time program is executing or the instrument is controlled through the LC-Net II/ADC,
another monitor screen is added. Figure 4.2 shows the monitor screen when the time program
is executed. See Chapter 5 for the time program setting and operation procedures.
When the instrument is connected to the LC-Net II/ADC, the time program and operation
methods are executed using the time program file number of 10. Therefore, the monitor
screen when the time program is executed is also displayed in the LC-Net II/ADC connection.

PRGM 1 INITIAL
0.0min

Figure 4.2 Monitor Screen when the Time Program is Executed

The terms of the monitor screen when the time program is executed and their meanings are
listed in Table 4.2.

Table 4.2 Monitor Screen Terms and Meanings

LCD screen Meaning
PRGM 1 The number of the time program being executed is displayed. The
figure shows that the program No. 1 is selected.
INITIAL Time program execution condition is displayed.
“INITIAL” is displayed in operation in the initial conditions, “RUN” in
step execution, and “FINISH” after the final step is complete.
0.0 min Elapsed time from starting the time program is displayed.

36
4.2.3 Monitor Screen in the LC-Net Connection
Figure 4.3 shows the monitor screen in the LC-Net II/ADC connection.
In the LC-Net II/ADC control, the time program and operation methods are executed using the
time program file number of 10. Therefore, the monitor screen when the time program is
executed is also displayed in the LC-Net II/ADC connection.

LC-Net CONNECT
PUMP # Pump Number

Figure 4.3 Monitor Screen in the LC-Net Connection

Table 4.3 Monitor Screen Terms and Meanings in the LC-Net Connection
LCD screen Meaning
LC-Net CONNECT It is displayed that the instrument is connected to the LC-Net II/ADC.
PUMP # Pump number connected to the LC-Net II/ADC is displayed.
The number (#) is the set pump number.

4.2.4 Monitor Screen Sleeping Function

The pump turns off the monitor screen and enters sleep status automatically when a key has
not been pressed for 5 minutes or more, depending on the setting. Even in the sleep status,
the pump flow and time program are executed as per setting values and program.
See Section [Link] for the setting.

The sleep status (where the monitor screen is off) is canceled and the current monitor screen
is displayed when any key is pressed.

4.3 Setting and Displaying the Parameters

This Section describes the basic parameter settings for analysis and measurement.
The time program mode (where analyses and measurements are carried out by changing the
parameter values as time passes) is shown in Chapter 5.
Press the [▼] key on the monitor screen to move to the parameter setting/selection screens.
Set the flow rate, maximum pressure, and minimum pressure. Also the operating pressure
without zero compensation can be displayed.
Press the [Clear] key to return to the monitor screen.

When setting the parameters or changing the screen, operable arrow keys (▲▼◄►) are
displayed in the rightmost position of the LCD screen.
Figure 4.4 shows the outline of key operations.

37
 1.000mL/min
Monitor screen
0.0MPa

[▼]

Setting Compositions
HPG RATIO
(Section 4.3.1)
L 100.0/ 0.0 R
[Clear]
[▼]
or
[◄] Setting Screen Display
FLOW RATE Mode

1.000mL/min Setting Flow Rate

(Section 4.3.2)
[▲] [▼]
[▲] [▼]

Setting Maximum and

MAX 40.0MPa
Minimum Pressures
MIN 0.0MPa
(Section 4.3.3)
[▲] [▼]

Displaying Operating
PRESS MONITER Pressure without Autozero
0.0MPa Compensation
(Section 4.3.4)
[▲] [▼]

Setting Mixing Speed for the

MIXING SPEED Mixing Unit
NORMAL (Section 4.3.5)

Figure 4.4 Screens for Setting and Displaying the Parameters

The parameter items, setting values, and meanings are shown in Table 4.4.

38
 Table 4.4 Parameter Items, Setting Values, and Meanings
Screen Setting range Description
HPG RATIO 0.0 to 100.0 (%) Sets the compositions.
FLOW RATE Depends on the pump model. Sets the flow rate.
MAX Depends on the pump model. Sets the maximum pressure.
If the operating pressure exceeds
the set maximum pressure, the
pump stops flowing.
MIN Depends on the pump model. Sets the minimum pressure.
If the operating pressure falls the set
minimum pressure, the pump stops
flowing.
PRESS MONITOR Displays the operating pressure
without autozero compensation.
MIXING SPEED Set the speed of the mixing unit,
which is installed in the center of the
unit.

See Section 4.3.6 for details on the setting range of each pump model.

39
4.3.1 Setting Compositions (HPG RATIO)
High pressure gradient, which the flow rates of two pumps are changed according to the
setting composition ratio, is performed in the pumps.
Set the composition ratio in this screen.
The example procedures for setting the composition ratio from L 100.0 / 0.0 R to 0 L 50.0 /
50.0 R are shown as follows.

(1) In the monitor screen, press the [▼] key to

HPG RATIO
display the composition setting screen.
L 100.0/ 0 .0 R 

[Menu/Enter]
(2) 1) Press the [Menu/Enter] key to flash the
composition for the right pump.
HPG RATIO
2) Press the [►] key to move the cursor
L 100.0/ 0 .0 R 
(underline) to the right. If the [►] key is
pressed in the rightmost digit, the cursor
moves to the leftmost digit. [▲][▼][►][◄]
Press the [◄] key to move the cursor to the
left.
HPG RATIO
During the setting, press the [Clear] key to
stop editing and return to the previous L 100.0/ 50.0 R 
value.
[Menu/Enter]
3) Press the [Menu/Enter] key to set the
composition for the right pump after
inputting all digits of the value. HPG RATIO
The composition for the left pump is L 50.0/ 50.0 R 
automatically calculated and displayed
after setting the right composition.

Press the [Clear] or [◄] key to return to the

monitor screen.

40
4.3.2 Setting Flow Rate (FLOW RATE)
Set the flow rate. See Section 4.3.6 for details on the setting range of each pump model.
The example procedures for setting the flow rate from 1.0 mL/min to 0.5 mL/min are shown as
follows.

(1) In the monitor screen, press the [▼] key twice

FLOW RATE
to display the flow rate setting screen.
1.000mL/min 

[Menu/Enter]
(2) 1) Press the [Menu/Enter] key to flash the flow
rate.
FLOW RATE
2) Press the [►] key to move the cursor
1.000mL/min
(underline) to the right. If the [►] key is
pressed in the rightmost digit, the cursor
moves to the leftmost digit. [▲][▼][►][◄]
Press the [◄] key to move the cursor to the
left.
FLOW RATE
During the setting, press the [Clear] key to
0.500mL/min
stop editing and return to the previous
value.
[Menu/Enter]
3) Press the [Menu/Enter] key to set the
flow rate after inputting all digits of the
FLOW RATE
value.
0.500mL/min
Press the [Clear] or [◄] key to return to the 
monitor screen.

41
4.3.3 Setting Maximum and Minimum Pressures (MAX/MIN)
The example procedures for setting the maximum pressure from 60.0 MPa to 45.0 MPa and
minimum pressure from 0.0 MPa to 5.0 MPa are shown as follows.

(1) In the monitor screen, press the [▼] key three MAX 60.0M Pa
times to display the maximum and minimum
MIN 0.0M Pa
pressure setting screen.

[Menu/Enter]

(2) 1) Press the [Menu/Enter] key to flash the

MAX 60.0M Pa
maximum pressure.
MIN 0.0M Pa
2) Press the [►] key to move the cursor

(underline) to the right. If the [►] key is
[▲][▼][►][◄]
pressed in the rightmost digit, the cursor
moves to the leftmost digit.
MAX 45.0M Pa
Press the [◄] key to move the cursor to the
left. MIN 0.0M Pa
During the setting, press the [Clear] key to 
[Menu/Enter]
stop editing and return to the previous
value.
MAX 45.0M Pa
3) Press the [Menu/Enter] key to set the
maximum pressure after inputting all digits MIN 0.0M Pa
of the value. 
4) Next, the minimum pressure flashes. [▲][▼][►][◄]

5) Press the [►] key to move the cursor

(underline) to the right. If the [►] key is MAX 45.0M Pa
pressed in the rightmost digit, the cursor MIN 5 .0MPa
moves to the leftmost digit. 
Press the [◄] key to move the cursor to the [Menu/Enter]

left.
During the setting, press the [Clear] key to MAX 45.0M Pa
stop editing and return to the previous MIN 5 .0MPa
value. 
6) Press the [Menu/Enter] key to set the
minimum pressure after inputting all digits
of the value.

Press the [Clear] or [◄] key to return to the

monitor screen.

42
4.3.4 Displaying Operating Pressure without Autozero
Compensation (PRESS MONITOR)
The operating pressure without autozero compensation is displayed. This is used when
pressure zero is adjusted.

Note: The pressure value in the monitor screen is sometimes different with that in this screen.
This causes when some pressure loaded at the power on and pressure autozero is
executed. In this case, again turn the instrument power on after opening the purge
valve.

PRESS MONITOR
0.0MPa

4.3.5 Setting Mixing Speed for the Mixing Unit (MIXING SPEED)
Set the mixing speed of the mixing unit.
The example procedures for setting the mixing speed from NORMAL to HIGH are shown as
follows.

(1) In the monitor screen, press the [▼] key five MIXING SPEED
times to display the mixing speed setting
NORMAL
screen.

[Menu/Enter]

(2) 1) Press the [Menu/Enter] key to flash the

MIXING SPEED
mixing speed.
NORMAL
2) Select the speed by the [▲][▼] keys.
During the setting, press the [Clear] key to

stop editing and return to the previous [▲][▼]
value.

MIXING SPEED
3) Press the [Menu/Enter] key to set the
speed. HIGH

[Menu/Enter]
Press the [Clear] or [◄] key to return to the
monitor screen.
MIXING SPEED
HIGH


43
4.3.6 Setting Ranges for Each Pump
Setting ranges for each pump are listed in Table 4.5.

Table 4.5 Setting Ranges for Each Pump

Model Item Minimum Maximum Increments
value value
Flow Rate (mL/min) 0.000 20.000 0.001
PU-4086-Binary Max (MPa) 0.0 50.0 0.1
Min (MPa) 0.0 50.0 0.1
Flow Rate (mL/min) 0.000 4.000 0.001
PU-4185-Binary Max (MPa) 0.0 70.0 0.1
Min (MPa) 0.0 70.0 0.1
Flow Rate (mL/min) 0.000 2.000 0.001
PU-4285-Binary Max (MPa) 0.0 130.0 0.1
Min (MPa) 0.0 130.0 0.1

44
4.3.7 Setting Flow Rate Using the [DIRECT] Key
After the [Drct] key is assigned to the flow rate and composition settings, pressing the [Drct]
key enables to change the flow rate and composition by directly moving from the monitor
screen to the setting screen. It is available during the pump flow.
See Section 6.6.1 for assigning the function to the [Drct] key. If other function is assigned to
this key, the operation is changed.

(1) In the monitor screen, pressing the [Drct] key 1.000mL/min

displays the flow rate and composition monitor
0.0MPa
screen and flashes the flow rate.

[Drct]

(2) 1) Press the [►] key to move the cursor

1.000mL/min
(underline) to the right. If the [►] key is
L 100.0/ 0 .0 R
pressed in the rightmost digit, the cursor
moves to the leftmost digit.

Press the [◄] key to move the cursor to the [▲][▼][►][◄]
left.
During the setting, press the [Clear] key to
0.500mL/min
stop editing and return to the previous
value. L 100.0/ 0 .0 R
2) Press the [Menu/Enter] key to set the 
[Menu/Enter]
flow rate after inputting all digits of the
value.
3) Next, the composition for the right pump 0.500mL/min
flashes. L 100.0/ 0 .0 R
4) Press the [►] key to move the cursor 
(underline) to the right. If the [►] key is [▲][▼][►][◄]
pressed in the rightmost digit, the cursor
moves to the leftmost digit. 0.500mL/min
Press the [◄] key to move the cursor to the L 100.0/ 50.0 R
left. 
During the setting, press the [Clear] key to [Menu/Enter]

stop editing and return to the previous

value. 0.500mL/min
5) Press the [Menu/Enter] key to set the L 50.0/ 50.0 R
composition after inputting all digits of the 
value.

45
4.4 Preparation of Mobile Phase Solvents
4.4.1 Solvent Filtration
The solvent used for HPLC analyses should be filtrated with a 0.45 µm or 0.2 µm membrane
filter before use.
Especially, narrow bore tubing and the column in which very small packing materials are
packed and narrow porous filters are installed are used in UHPLC (Ultra High-performance
Liquid Chromatography) and RHPLC (Rapid Separation High-performance Liquid
Chromatography). If mall particles exist in mobile phase solvents, the tubing or column filters
may be clogged. To prevent clogging, the mobile phases should be filtrated with a 0.2 µm
membrane filter before use.

46
4.4.2 General Considerations for Solvent Replacement
Note: The solvent in the instrument shipped from the factory is methanol.

Be sure to replace the solvent being replaced in the pump for the solvent that is intermiscible
with the former at any composition. Figure 4.5 shows the compatibility of solvents used
frequently for liquid chromatography.

Aqueous Solvents

Buffer
Salt solvents

Water

Ethanol
Organic IPA
Solvents (Acetone)

Acetonitrile
Methanol

Chloroform
Ethyl acetate

n-Hexane
Isooctane

Figure 4.5 Compatibility of Solvents

CAUTION
If the solvents replaced are not inter-miscible with the new solvents in any composiiton,
salts may precipitate in the instrument, which may cause the instrument to malfunction,
shorten the service life, or deteriorate the performance.

47
4.5 Power OFF
Press the power button on the operation panel. The power button LED is red. In this condition,
the instrument is off but the main power is on (standby status).
The set parameters and time programs stored in files are stored in memory in the instrument.
Those values are used when power is turned on the next time.

4.5.1 Turning the Main Power OFF

The power button on the operation panel is a software switch. When the power is turned off
with this key, power for instrument operations is turned off (standby status).
The power button LED is red. To turn off the main instrument power, turn off the main power
switch on the rear panel of the instrument (see Section 2.5). The power button LED goes out.

Note: Be sure to turn off the main power before moving the instrument.

4.6 Leaving the Instrument Idle for Extended Periods

If the instrument is not used for long periods (about three days or more) after using water,
buffer, and organic solvent containing halogen radical as mobile phase, replace the solvent
inside of the instrument with stable solvent. Methanol or acetonitrile is the best.

CAUTION
If the solvent is not replaced, salts may precipitate, mold may be produced and the
instrument may be damaged.

48
5. Operations in Time Program Mode
5.1 Outline of Operations in Time Program Mode
In the time program mode, flow rate, maximum pressure, and minimum pressure can be
changed with time. The maximum number of time program steps is 64, and the number of
program files is 10.
The flowchart below shows the outline of the time program mode operations.

Set the time program steps in the Chromatography Data System (e.g. ChromNAV Ver.2)
when the LC system is controlled with the Chromatography Data System.
Use the time program function of the pump when the system is not controlled with the
Chromatography Data System.

The following flowchart shows the flow until executing the time program.

Changing into time program setting screen (Section 5.2)

Setting the program number (Section 5.3)

Setting the initial conditions (Section 5.4)

Setting the time program steps (Section 5.5)

Starting the time program (Section 5.7)

49
5.2 Time Program Setting Screens
Figure 5.1 shows how to change the monitor screen into various time program screens.

1.000mL/min
Monitor screen
0.0MPa

[▼]

PRGM FILE NO. Setting program number

1 (Section 5.3)

[Clear]
or [▲] [▼]
[◄]

PRGM FILE 1 Setting initial conditions

(Section 5.4)
INITIAL

[▲] [▼]
[▲] [▼]

PRGM FILE 1 Setting time program steps

TIME PR OGRAM (Section 5.5)

[▲] [▼]

PRGM FILE 1 Others

OTHERS (Section 5.6)

Figure 5.1 Changing into Time Program Setting Screen

50
5.3 Setting Program File Number
It is possible to set 10 types of time programs (1 to 10).
The example procedures for changing the program file number from 1 to 2 are shown as
follows.

(1) In the monitor screen, press the [◄] to display

PRGM FILE NO.
the program file number setting screen.
1

[Menu/Enter]

(2) 1) Press the [Menu/Enter] key to flash the PRGM FILE NO.
program file number.
1
2) Set the number by the [▲][▼] keys.
During the setting, press the [Clear] key to
stop editing and return to the previous [▲][▼]
value.
3) Press the [Menu/Enter] key after inputting
PRGM FILE NO.
all digits to set the file number.
2
Press the [Clear] or [◄] key to return to the
monitor screen. [Menu/Enter]

PRGM FILE NO.

2

5.4 Setting the Initial Conditions

Set the composition, flow rate, maximum pressure, and minimum pressure as the initial
conditions.
See Section 4.3 for the setting.

5.5 Setting Time Program

5.5.1 Editing Steps
Set each step of the time program. A maximum of 64 steps can be set. Set the time for
executing functions (step time), select the function to be changed at that time, and set the
function in sequence.
Set the composition (HPG RATIO), flow rate (Flow Rate), maximum pressure (Press Max),
and minimum pressure (Press Min) in each step.

51
(1) In the time program setting screen, press the
PRGM FILE 1
[Menu/Enter] key to display the step editing
TIME PR OGRAM
screen.

(2) 1) Press the [Menu/Enter] key to flash the [Menu/Enter]

time of executing the function.
2) Enter the time by the [▲][▼] keys. STEP 1
Press the [►] key to move the cursor
(underline) to the right. If the [►] key is
pressed in the rightmost digit, the cursor [Menu/Enter]
moves to the leftmost digit.
Press the [◄] key to move the cursor to the STEP 1 0.0min
left.
During the setting, press the [Clear] key to
stop editing and return to the previous
[▲]
value.
Press the [Menu/Enter] key to set the STEP 1 1.0m in
maximum pressure after inputting all digits
of the value.
[Menu/Enter]
(3) 1) After setting the time, the function to be
executed is displayed and flashes.
STEP 1 1.0m in
2) Select the function, composition (HPG
RATIO), flow rate (FLOW RATE), HPG RATIO
maximum pressure (PRESS MAX), or [Menu/Enter]
minimum pressure (PRESS MIN) by the
[▲][▼] keys.
3) Press the [Menu/Enter] key to set the STEP 1 1.0m in
function. 100.0/ 0.0 R
4) The set value of the function flashes. Enter
[▲][▼][►][◄]
the value by the [▲][▼][►][◄] keys.
5) Press the [Menu/Enter] key. Input of that
step is set. When the input is set, setting is STEP 1 1.0m in
stored automatically in the time program of L 100.0/ 50.0 R
the selected file number.
[Menu/Enter]

STEP 1 1.0m in
L 50.0/ 50.0 R
[▼]

52
 (4) Press the [▼] key and set steps of Step 2 and
so forth.

STEP 2

5.5.2 Changing Step

Press the [Menu/Enter] key in a validated step to reedit the step.
The time and function value can be changed here. The function cannot be changed. Delete
the step and insert a step to change the function (see Section 5.5.3).

5.5.3 Inserting and Deleting Step

Outline of the step insertion and deletion screen is shown below.

STEP 1 1.0m in
Step screen
1.000mL/min
[►]

PRGM EDIT Step insertion

INSERT STEP
[◄]
[▲] [▼]

PRGM EDIT Step deletion

DEL THIS STEP

[▲] [▲] [▼] [▼]

The following steps are

PRGM EDIT
deleted completely.
DEL FOL STEPS

Figure 5.2 Step Insertion and Deletion

53
(1) The step insertion procedures are as shown below.
1) Display the step insertion screen (see Figure 5.2) for the step to be inserted.
2) Press the [Menu/Enter] key to display the step editing screen.
3) Input as shown in Section 5.5.1.
4) Press the [◄] key to close the editing screen. Steps are rearranged in the order of
time automatically.

(2) The procedures for deleting one step are as shown below.
1) Display the step deletion screen (see Figure 5.2) for the step to be deleted.
2) Press the [Menu/Enter] key to display the confirmation screen to execute the
deletion.
3) Press the [Menu/Enter] key to delete the step. Press the [◄] key to cancel deletion.

(3) The procedures for deleting all steps after a specified step are as shown below.
1) Display the screen for deleting all steps after a specified step (see Figure 5.2) for the
first step to be deleted.
2) Press the [Menu/Enter] key to display the confirmation screen to execute the
deletion.
3) Press the [Menu/Enter] key to delete the steps. Press the [◄] key to cancel deletion.

54
5.6 Other Settings
The following shows the overview.

PRGM FILE 1
Other screen
OTHERS

[Menu/Enter]
or
[►]
[◄]

OTHERS Deleting time program

DEL PRGM FILE (Section 5.6.1)

[▲] [▼]

PRGM EDIT Copying time program

COPY PRGM FILE (Section 5.6.2)

Figure 5.3 Other Setting Screen

5.6.1 Deleting Time Program

The procedures for deleting the time program of a currently used file number are as shown
below.

(1) Select the time program of the file number to be deleted (see Section 5.3).
(2) Display the time program deletion screen (see Section 5.6).
(3) Press the [Menu/Enter] key to display the confirmation screen to execute the deletion.
(4) Press the [Menu/Enter] key to delete the steps. Press the [◄] key to cancel deletion.

5.6.2 Copying Time Program

Copy the contents of the time program of the currently used file number to the time program of
the other file number. The procedures are as shown below.

(1) Display the time program copy screen (see Section 5.6).
(2) Press the [Menu/Enter] key to display the copy execution screen.
(3) Enter the time program number to copy and press the [Menu/Enter] key to execute
copying.

55
5.7 Time Program Operations
5.7.1 Starting Initial Conditions
Press the [Run/Stop] key once on the monitor screen to start the initial conditions.
While executing the initial conditions, the [Run/Stop] key LED blinks green and the program
number, time program execution status (INITIAL), and elapse time (0.0 min) are displayed on
the time program monitor screen (shown in Section 4.2.2).

5.7.2 Executing Time Program

Press the [Run/Stop] key once while starting the initial conditions, the time program starts.
While executing the time program, the [Run/Stop] key LED blinks green and the program
number, time program execution status (RUN), and elapse time are displayed on the time
program monitor screen.
When the time program has been executed to the last step, “FINISH” is displayed in the step
number and the conditions are maintained. The [Run/Stop] key LED remains on.

5.7.3 Stopping Time Program

Press the [Run/Stop] key LED once while executing the time program. The time program
stops and the initial conditions are set automatically.

Note: The time program can also be started and reset with the RESET/RUN terminals on the
rear panel.
While executing the initial conditions, start the time program by momentarily closing
the terminals.
While executing the time program, time is reset to zero and the time program restarts
by momentarily closing the terminals again.
The input should be a contact closure signal (pulse) with 25 V and 10 mA or more.

5.7.4 Temporarily Parameter Changes while the Time Program is

Running
It is possible to change the settings of the flow rate, maximum pressure, and minimum
pressure while the time program is executed. The setting procedures are the same as shown
in Section 4.3.

Note: If the setting is changed while the time program is executed, time elapses while
maintaining the changed value. For example, if the flow rate is changed while the time
program is executed, it is not changed automatically even if the flow rate is set in the
next step.

56
6. Setting Instrument Parameters
6.1 Commands
Press the [▲] key on the monitor screen to display the Command menu screens. In
COMMAND, purging execution, setting pump ON timer and OFF timer, and setting instrument
power OFF timer can be executed.
The outline of key operations in COMMAND is shown in Figure 6.1 and each command item
is described in Table 6.1.

1.000mL/min
Monitor Screen
0.0MPa

[▲]

SOLVENT PURGE Executing Purging

STOP (Section 6.1.1)
[Clear]
[▲] [▼]
or
[◄]
POWER OFF TIMER Setting Instrument Power

OFF OFF Timer

(Section 6.1.2)
[▲] [▼]
[▲] [▼]

PUMP ON TIME R Setting Pump ON Timer

OFF (Section 6.1.3)

[▲] [▼]

PUMP OFF TIMER Setting Pump OFF Timer

(Section 6.1.4)
OFF

Figure 6.1 Command Menu Screens

57
 Table 6.1 Description of Commands
Display Description
SOLVENT PURGE Executes purging (solvent replacement function). This function is
used to replace the solvents in the pump.
POWER OFF TIMER Sets the instrument power OFF timer. When the set time has been
elapsed, the instrument power is turned OFF.
Setting range: OFF, 0.1 to 99.9 hours
PUMP ON TIMER Sets the pump ON timer. When the set time has been elapsed, the
pump starts flowing.
Setting range: OFF, 0.1 to 99.9 hours
PUMP OFF TIMER Sets the pump OFF timer. When the set time has been elapsed, the
pump stops flowing.
Setting range: OFF, 0.1 to 99.9 hours

6.1.1 Executing Purging (Solvent Replacement Function)

(SOLVENT PURGE)
This function is used to replace the solvents in the pump. Use of this function enables speedy
solvent replacement in the pump.

Note: When purging is executed, large volume of solvent goes out from the purge valve.
Before purging, check that the wasted solvent can be collected to a bottle without any
solvent leak or scattering from the pump. In addition, note a tubing disconnection.

58
(1) In the screen shown in the right figure, press
SOLVENT PURGE
the [Menu/Enter] key to flash “STOP” display.
STOP

(2) Change the display to “RUN PURGE” by the

SOLVENT PURGE
[▲][▼] keys. And then press the [Menu/Enter]
key. STOP

[▲] [▼]

(3) The display is changed to purging flow rate SOLVENT PURGE

setting screen. RUN PURGE
Set the flow rate. See Section 4.3.2 for setting
the flow rate. [Menu/Enter]

PURGE FLOW
4.000mL/min
(4) After setting the flow rate, open the purge
valve. Check that the waste line accepts [Menu/Enter]

enough volume for wasting, and then press the

[Pump] key. OPEN VALVE
PRESS PUMP
[Pump]

(5) Purging will start.

Pump will flow at the set flow rate and the
RUNNING PURGE
composition of L/R = 50/50.
During purging, press the [Pump] key to halt the 4.000mL/min
flow. Press the [Clear] key to cancel purging.

(6) When purging at a specified flow rate has DONE PURGE

completed, automatically stop the pump flow
and change the display shown in the right
figure.

(7) Press the [Clear] key to return to the monitor

screen.

6.1.2 Setting Instrument Power OFF Timer (POWER OFF TIMER)

When the set time has been elapsed, the instrument power is turned OFF.
The setting range is OFF and 0.1 to 99.9 hours.

59
6.1.3 Setting the Pump ON Timer (PUMP ON TIMER)
When the set time has been elapsed, the pump starts flowing.
The setting range is OFF and 0.1 to 99.9 hours.

6.1.4 Setting the Pump OFF Timer (PUMP OFF TIMER)

When the set time has been elapsed, the pump stops flowing.
The setting range is OFF and 0.1 to 99.9 hours.

6.2 Instrument Parameters (MENU)

When the [Menu/Enter] key is pressed in the monitor screen, the instrument parameter menus
are displayed. The items on the menu screens and details are as shown in Table 6.2.
Figure 6.2 is an overview of the instrument parameter (MENU) key operations.

Table 6.2 Items on Menu Screens and Details

Item Description
1 CONFIGURATION Sets the pressure unit. See Section 6.3 for the settings.
2 ACCESSORY Confirmation/setting of the mixing unit and degassing
unit installed in the pump.
See Section 6.4 for the settings.
3 MAINTENANCE Confirmation of the instrument history, setting and
confirming of the flow rate calibration factors and
pressure calibration factors, and the error output
method can be set. See Section 6.5 for the settings and
confirmation.
4 TOOL Assignment of the DIRECT KEY operation, switching
the DISPLAY MODE and BUZZER MODE, setting of
the optional units can be done. See Section 6.6 for the
settings.
5 INFORMATION Confirmation and setting of the instrument information
and pump number. See Section 6.7 for the settings and
confirmation.

60
 1.000mL/min Monitor Screen
0.0MPa

[Menu/Enter]

[Clear] Menu
(Section 6.3)
or CONFIGURATION
[◄]
[▲] [▼]

Menu (Section 6.4)

ACCESSORY

[▲] [▲] [▼] [▼]

Menu
(Section 6.5)
MAINTENANCE

[▲] [▼]

Menu (Section 6.6)

TOOL

[▲] [▼]

Menu
(Section 6.7)
INFORM ATION

Figure 6.2 Instrument Parameter Menu Screens

61
6.3 Configuration Items (CONFIGURATION)
Set the pressure unit. Figure 6.3 is an overview of the key operations in CONFIGURATION.

1.000mL/min
Monitor Screen
0.0MPa

[Clear] [Menu/Enter]

MENU
CONFIGURATION

[◄] [Menu/Enter]

CONFIGURATION Setting Pressure Unit

PRESS UNIT (Section 6.3.1)

Figure 6.3 CONFIGURATION Menu Screens

6.3.1 Setting Pressure Unit (PRESS UNIT)

Set pressure unit. Select MPa, at (kgf/cm2), psi, or bar as pressure unit.

CONFIGURATION
PRESS UNIT

[Menu/Enter]

PRESS UNIT
MPa

62
6.4 Accessory Items (ACCESSORY)
Confirm and set the information of the mixing unit and degassing unit installed in the pump.
Figure 6.4 is the overview of key operations in ACCESSORY.

1.000mL/min
Monitor Screen
0.0MPa

[Clear]

MENU
ACCESSORY

[◄] [Menu/Enter]

Confirming/setting the
ACCESSORY
Information of the Mixing
MIXING UNIT
Unit (Section 6.4.1)

[▲] [▲] [▼] [▼]

Confirming/setting the
ACCESSORY Information of the
DG#1 UNIT Degassing Unit
(Section 6.4.2)

Figure 6.4 ACCESSORY Menu Screens

6.4.1 Confirming/setting the Information of the Mixing Unit

(MIXING UNIT)
In this screen, input the mixing chamber volume and maximum pressure of the mixing
chamber, and set mixing speed (value) when the mixing speed is set to “NORMAL” and
mixing speed (value) when the mixing speed is set to “HIGH”.
Figure 6.5 is the overview of the MIXING UNIT key operations.
The items on the MIXING UNIT screens and details are as shown in Table 6.3.

63
 1.000mL/min
Monitor Screen
0.0MPa

[Menu/Enter]

MENU
ACCESSORY

[Menu/Enter]

ACCESSORY
MIXING UNIT

[Menu/Enter]

[◄] CHAMBER VOLUME

1500uL

[▲] [▼]

CHAMBER MAXIMUM
PRESS 50.0MPa

[▲] [▼]

MIXING SPEED
NORMAL:1100 rpm

[▲] [▼]

MIXING SPEED
HIGH:2000 rpm

Figure 6.5 Overview of the MIXING UNIT Key Operations

64
 Table 6.3 Items in MIXING UNIT Screens and Details
Display Setting range Description
CHAMBER VOLUME 0 to 30000 µL Sets the mixing chamber volume
installed into the mixing unit.
CHAMBER MAX PRESS 0.0 to 130.0 MPa Sets the maximum pressure of mixing
chamber installed into the mixing unit.
MIXING SPEED NORMAL 400 to 5100 rpm Sets the rotation speed for the setting,
NORMAL.
MIXING SPEED HIGH 400 to 5100 rpm Sets the rotation speed for the setting,
HIGH.

6.4.2 Confirming/setting the Information of the Degassing Unit

(DG#1 UNIT)
In this screen, input the channel number of the degassing unit installed in the pump.
Figure 6.6 is the overview of the DG UNIT key operations.
Four and five channel degassing units are available.

ACCESSORY
DG#1 UNIT

[◄] [Menu/Enter]

DG#1 UNIT Degassing Unit Confirmation

4-LINE Screen

Figure 6.6 Overview of the DG UNIT Key Operations

65
6.5 Maintenance Items (MAINTENANCE)
Confirm the instrument history and confirm and set the instrument calibration factors.
Figure 6.7 is the overview of key operations in MAINTENENCE.

1.000mL/min
Monitor Screen
0.0MPa

[Clear]

MENU
MAINTENANCE

[◄] [Menu/Enter]

MAINTENANCE Instrument History

HISTORY (Section 6.5.1)

[▲] [▲] [▼]

MAINTENANCE Setting Instrument

Product Parameters
PRODUCT PAR AM
(Section 6.5.2)

[▲] [▼] [▼]

Setting Error-related
MAINTENANCE
Parameters
ERROR SETTING
(Section 6.5.3)

Figure 6.7 MAINTENANCE Menu Screens

6.5.1 Instrument History (HISTORY)

Confirm or edit the plunger seal replacement information, confirm the number of the plunger
driving, and confirm or edit the total power ON time for both right and left pumps in HISTORY.

(1) Plunger Seal Replacement Information (L SEAL REPLACE/R SEAL REPLACE)

Input date in SEAL REPLACE. When the plunger seal is replaced, input the maintenance
date. It is saved in the pump. It can be set for both right and left pumps individually.

66
(2) The Number of the Plunger Driving (L PLUNGER STROKE/R PLUNGER STROKE)
Confirm and set the number of the plunger driving in PLUNGER DRIVE. It can be set and
confirmed for both right and left pumps individually.
This is useful for the instrument maintenance management by setting number to zero
when the plunger seal is replaced.

(3) The number of the Total Power ON Time (TOTAL ON TIME)

Confirm the total power ON time in TOTAL ON TIME.

6.5.2 Setting Instrument Product Parameters (PRODUCT PARAM)

Confirm or set the flow rate calibration factors and pressure calibration factors for both right
and left pumps in PRODUCT PARAM. Use this function to reset the instrument parameters.
Note: At the shipment, the parameter values are written in a label, which is attached on the
backside of the front panel.

(1) Confirming and Setting L FLOW COMP / R FLOW COMP

FLOW COMP is the flow rate calibration factor at low flow rate and low operating
pressure. Confirm or set the factor in this screen.

(2) Confirming and Setting L FLOW FACTOR / R FLOW FACTOR

FLOW FACTOR is the flow rate calibration factor at high flow rate and low operating
pressure. Confirm or set the factor in this screen.

(3) Confirming and Setting L PRESS COMP / R PRESS COMP

PRESS COMP is the flow rate calibration factor at middle flow rate and high operating
pressure. Confirm or set the factor in this screen.

(4) Confirming and Setting L PRESS FACTOR H / R PRESS FACTOR H

PRESS FACTOR H is the flow rate calibration factor at high flow rate and high operating
pressure. Confirm or set the factor in this screen.

(5) Confirming and Setting L PRESS FACTOR L / R PRESS FACTOR L

PRESS FACTOR L is the flow rate calibration factor at low flow rate and high operating
pressure. Confirm or set the factor in this screen.

(6) Confirming and Setting L [Link] FLOW / R [Link] FLOW

[Link] FLOW is used to set the flow rate for PRESS FACTOR H. Confirm or set the
factor in this screen.

(7) Confirming and Setting PRESS PARAM

PRESS PARAM is the pressure calibration factor. Confirm or set the factor in this screen.

67
(8) Confirming and Setting PRESS ADJUST
PRESS ADJUST is the value when the pressure is calibrated using a standard pressure
meter.

6.5.3 Setting Error-related Parameters (ERROR SETTING)

Confirm the current leak sensor output value and set the error judgment threshold for solvent
leak, and set the criteria for the number of the plunger driving in ERROR SETTING.

(1) Setting Leak Sensor Detection Level (LEAK LEVEL)

Confirm the detection level of the leak sensor and set the threshold for leak detection and
error judgment in LEAK LEVEL. If the detected value (ACT) exceeds the threshold value
(SET), “LEAK IN CELL” message is displayed and an error is output.
If the threshold value is set to “0”, error judgment is not performed, regardless of the
detected value (ACT).

(2) Setting PRESS WARNING

Set the threshold of pressure in PRESS WARNING. If the operating pressure exceeds
the threshold value, its information is send to the Chromatography Data System such as
ChromNAV Ver.2.

(3) Setting EXTERNAL INPUT

When the pump flow stops due to trouble stop, set the operation performed external input
terminals at rear panel. Set “IGNORE”, and “RESET RUN” or “PUMP ON” external input
signals are ignored. Set “ERROR CLEAR”, “RESET RUN” or “PUMP ON” external input
signals are run. When “RESET RUN” or “PUMP ON” external input signals are input, a
pump stop is cleared and time program is run.

(4) Setting WARNING LEVEL - PLUNGER

Set the thresholds for the number of the plunger driving for both L PUMP and R PUMP,
which can use the time of maintenance of the plunger section. If the number exceeds the
threshold value, “TIME TO MAINTE PLUNGER STROKE” message is displayed.

68
6.6 TOOL Items (TOOL)
Assign the [Drct] key operation, set the screen display mode, and set the error sound mode in
TOOL.
Figure 6.8 is the overview of key operations in TOOL.

1.000mL/min Monitor Screen

0.0MPa

[Menu/Enter]

MENU
TOOL

[Menu/Enter]

Assigning [Drct] Key

[◄] TOOL
Operation
DIRECT KEY
(Section 6.6.1)

[▲] [▼]

TOOL Setting Display Mode

DISPLAY M ODE (Section 6.6.2)

[▲] [▲] [▼] [▼]

TOOL Setting Error Sound

ERROR BEEP (Section 6.6.3)

[▲] [▼]

TOOL Setting SF-NAV Mode

SF-NAV M ODE (Section 6.6.4)

[▲] [▼]

Setting Event Signal

TOOL
Output Unit
EVENT UNIT
(Section 6.6.5)

Figure 6.8 TOOL Menu Screens

69
6.6.1 Assigning [Drct] Key Function (DIRECT KEY)
Assign the [Drct] key operation in DIRECT KEY. The following functions can be assigned.

Table 6.4 Key Operation that can be Assigned to the [Drct] Key
Selection item Description
MONITOR EDIT Pressing the [Drct] key enables to set the flow rate directly from the
monitor screen (Default setting).
SET FLOW RATE Press the [Drct] key to move to the flow rate setting screen.
SET P MAX/P MIN Press the [Drct] key to move to the maximum and minimum pressure
setting screen.

6.6.2 Setting Display Mode (DISPLAY MODE)

Set the display power and automatic switching of the monitor screens in DISPLAY MODE.
Figure 6.9 is the overview of DISPLAY MODE key operations.

TOOL
Display setting
DISPLAY M ODE

[◄] [Menu/Enter]

BACKLIGHT: ON
Details of display setting
SWITCH: MANUAL

Figure 6.9 Overview of the DISPLAY MODE

[Link] Setting Backlight (BACKLIGHT)

Set whether to turn off the LCD backlight automatically in BACKLIGHT. The selection items
and operation details are as shown below.

Table 6.5 Backlight Set Items and Details

Selection item Description
ON Backlight is always turned on (Default setting).
AUTO Backlight is turned off if no key is pressed for 5 minutes. Press a key
other than the power button to turn on the backlight again.

70
[Link] Setting Automatic Monitor Screen Switching (SWITCH)
The pump has several monitor screens.
Set the automatic monitor screen switching in SWITCH. The selection items and operation
details are as shown below.

Table 6.6 Monitor Screen Setting and Details

Selection item Description
MANUAL Press the [►] key to manually switch the display (Default setting).
AUTO Monitor screen display is switched automatically (at two-second
intervals). Press any key to stop automatic switching. Switching
restarts 5 seconds later.

6.6.3 Setting Error Sound (ERROR BEEP)

Set the error sound mode when an error occurs in ERROR BEEP. The selection items and
operation details are as shown below.

Table 6.7 Error Sound Setting and Details

Selection item Description
CONTINUE Error sound is generated until an error is clear (Default setting).
5-TIMES Error sound is generated five times and then stops.

6.6.4 Setting SF-NAV MODE (SF-NAV MODE)

When the pump is controlled by the supercritical fluid chromatography control program,
SF-NAV, sets this function to ON.
The selection items and operation details are as shown below.

Table 6.8 SF-NAV Mode Setting and Details

Selection item Description
OFF Normally, set the mode to OFF (Default setting).
ON When the pump is controlled by the supercritical fluid chromatography
control program, SF-NAV, sets this function to ON.

6.6.5 Setting Event Signal Output Unit (EVENT UNIT)

When the optional event signal output unit is installed to the pump, change this mode setting.
The selection items and operation details are as shown below.

Table 6.9 Event Signal Output Unit Setting and Details

Selection item Description
NOT INSTALLED When the optional event signal output unit is not installed, set this
mode to “NOT INSTALLED” (Default setting).
INSTALLED When the optional event signal output unit is installed, set this mode
to “INSTALLED”.

71
6.7 Information Items (INFORMATION)
Confirm the instrument name and firmware version and type, and confirm or set the serial
number in INFORMATION.
Figure 6.10 is the overview of key operations in INFORMATION.

1.000mL/min
Monitor Screen
0.0MPa

[Clear] [Menu/Enter]

MENU
INFORM ATION

[◄] [Menu/Enter]

INFORM ATION Confirming Instrument

Name
MODEL
(Section 6.7.1)

[▲] [▼]

Confirming Firmware
INFORM ATION
Version/Type
VERS ION/TYPE
(Section 6.7.2)

[▲] [▲] [▼] [▼]

Confirming & Setting

INFORM ATION Serial Number
SERIAL NUMBER (Section 6.7.3)

[▲] [▼]

Confirming and Setting

INFORM ATION
Pump Number
PUMP NUMBER
(Section 6.7.4)

Figure 6.10 INFORMATION Menu Screens

72
6.7.1 Confirming the Instrument Name (MODEL)
Confirm the instrument name in MODEL.

6.7.2 Confirming the Firmware Version/Type (VERSION/TYPE)

Confirm the instrument firmware program version and type in VERSION/TYPE.

6.7.3 Confirming and Setting Instrument Serial Number

(SERIAL NUMBER)
Confirm and set the serial number in SERIAL NUMBER. The serial number is printed on the
model seal attached on the rear of the instrument and the seal on the top of the front panel.

6.7.4 Confirming and Setting Pump Number (PUMP NUMBER)

Confirm and set the pump number in PUMP NUMBER.
When two or more numbers of pump are controlled from the Chromatography Data System
such as ChromNAV Ver.2 via the LC-Net, the pump number should be set to each pump.
Set the different pump number to each pump in the system.
When the pump number is changed, the pump should be turned OFF and ON again to
activate the number.

(1) When one pump is being used in the system, the pump is usually set as Pump 1.

(2) When two or more pumps are being used in the system and high-pressure gradient is
performed by using the Chromatography Data System such as ChromNAV Ver.2, set
the pump number for each pump to 1 to 4 in order.

(3) When two or more pumps are being used in the system and each pump flows at
different flow rate by using the Chromatography Data System such as ChromNAV Ver.2,
set the pump number for each pump to 1 to 4 in order.

Maximum controllable pump number from the Chromatography Data System is 4.

Note: Never set the same pump number to the pumps in the system. Or the connection and
control from the Chromatography Data System may cause the problem.

73
7. Error/Information Messages and Troubleshooting
7.1 Error during Self-Diagnosis Test after Power Up
7.1.1 Self-diagnosis Test Items
The pump checks the following items and initializes the setting automatically when the power
is turned on:

(1) Checks backed-up battery

(2) Reads the setting values from the volatile memory

(3) Reads and writes the setting values form/to the nonvolatile memory
(4) Checks the pressure sensor and autozeros the pressure value display

When a problem is detected during the self-diagnosis test, an error message is displayed and
the self-diagnostic test stops. See Section 7.1.2 and the following sections for
troubleshooting.

7.1.2 BATTERY IS DEAD

The backed-up memory is firstly checked as an item of the self-diagnosis. This message is
displayed when the backup battery is run out. Turn off the power and turn it on again. If the
same message is displayed after turning on the power again, the backup battery should be
replaced. Contact your local JASCO distributor.
However, it is possible to cancel this message temporarily and restart the self-diagnosis tests
by pressing the [Clear] key.
Note: Wait 30 seconds or more after turning off the power before turning on the pump again.

7.1.3 BACKUP ERROR

This message is displayed when all parameters, including those stored in nonvolatile memory,
are initialized. Turn off the power and turn it on again if this message is displayed. If the same
message is displayed after turning on the power again, contact your local JASCO distributor.
However, it is possible to cancel this message temporarily and restart the self-diagnosis tests
by pressing the [Clear] key.
Important parameters for using the instrument are stored in the nonvolatile memory. When
this error occurs, default values are set. Set the flow rate calibration factors and pressure
calibration factor again to keep using the instrument. The parameters required for resetting
are shown on the parameter label put on the backside of the front panel. Table 7.1 shows the
parameters stored in the nonvolatile memory.
Note: Wait 30 seconds or more after turning off the power before turning on the pump again.

74
 Table 7.1 Parameters Stored in Nonvolatile Memory
Parameter name Written on Setting procedure
Serial No. Top of front panel See Section 6.7.3
Pump number - See Section 6.7.4
Flow rate calibration factors Parameter label put See Section 6.5.2
(for Right and Left Pump) on the backside of
(Flow Comp, Flow Factor, Press Comp, Press the front panel
Factor L, Press Factor H, and P. Factor Flow)
Pressure calibration factor Parameter label put See Section 6.5.2
(for Right and Left Pump) on the backside of
(Press Param) the front panel
The number of the plunger driving - See Section 6.5.1
(for Right and Left Pump)
Plunger replacement date - See Section 6.5.1
(for Right and Left Pump)
Pressure unit setting - See Section 6.3.1
Leak sensor detection level setting - See Section 6.5.3
Pressure setting for Press Warning - See Section 6.5.3
SF-NAV Mode setting - See Section 6.6.4
Mixing chamber volume setting Parameter label put See Section 6.4.1
on the backside of
the front panel
Mixing chamber maximum pressure setting Parameter label put See Section 6.4.1
on the backside of
the front panel
Event unit setting - See Section 6.6.5

7.1.4 NVRAM CORRUPTED

This message is displayed if the nonvolatile memory is broken and setting values cannot read
or write. Turn off the power and turn it on again if this message is displayed. If the same
message is displayed after turning on the power again, contact your local JASCO distributor.
Note: Wait 30 seconds or more after turning off the power before turning on the pump again.

7.1.5 SET PARAMETERS

This message is displayed when the measurement and instrument parameters stored in the
volatile memory are initialized. Turn off the power and turn it on again if this message is
displayed. If the same message is displayed after turning on the power again, the backup
battery should be replaced. Contact your local JASCO distributor.
However, it is possible to cancel this message temporarily and restart the self-diagnosis tests
by pressing the [Clear] key.
The parameters, except those shown in Table 7.1, are reset to the default values. Set the
parameters again to keep using the instrument.
Note: Wait 30 seconds or more after turning off the power before turning on the pump again.

75
7.1.6 CAN’T RESET PRESS SENSOR
This message is displayed when the pressure is outside the specified value (0.0 ± 0.7 MPa).
However, the self-diagnosis test can temporarily be resumed by pressing the [Clear] key.

Possible causes

(1) Pump pressure is too high.

(2) Time variation in the pressure sensor and shifts the zero point.
(3) Malfunction in the pressure sensor or pressure sensor signal circuit.

What to do

Open the purge valve, and then turn the power OFF and ON again. If the error has gone, it
was probably due to the pump power being turned ON while the pump was pressurized
(Cause 1).
If the error occurs again, time variation in the pressure sensor (Cause 2) or the signal circuit
(Cause 3) is the most likely causes.
If an error occurs due to time variation in the pressure sensor, use the zero adjustment
trimmer to zero the sensor, then turn the power ON again. If the problem continues, the
pressure sensor or the pressure sensor signal circuit is not working correctly. Contact your
local JASCO distributor.

7.2 Errors during Operation

When the problem is detected during operation, operation stops immediately. Then,

(1) Alarms buzzer sounds. It depends on the setting in [TOOL] - [ERROR BEEP] (see Section
6.6.3).
(2) The corresponding error message is displayed.
(3) The contact closure signal (close) is output from “STOP OUT” terminals.

Press the [Clear] key to clear the error message and open the STOP OUT signal. And then
the instrument is initialized and the monitor screen is displayed.
If the error causes are not removed, the error message appears again.

7.2.1 SOLVENT LEAK

Solvent sensors for detecting solvent leaks are equipped in the pump (see Sections 2.3 and
2.4). If it detects solvent leaks and the sensor signal exceeds the setting value (see Section
6.5.3), this error occurs.
If any solvent leak is found, remove the solvents. And press the [Clear] key to clear the error.

76
Possible causes for solvent leaks:

(1) Solvent leaks from the connection points of the tubing in the instrument.
(2) Solvent leaks from the pump head sections.
(3) Purge valve is not closed completely and solvent leaks from the drain tubing.
(4) Leak sensor detection level setting is too small.
(5) Mulfunciton of leak sensors

What to do

(1) Connect the tubing in the instrument firmly and check if solvent leaks are found.
(2) Check if the purge valve is closed completely.
(3) Referring Section 6.5.3, increase the leak sensor detection level.
(4) If solvent leaks are found, replace the plunger seal with the new one. See Sections 8.5,
8.10, and 8.13 for the replacement.
(5) The pressure sensor signal circuit may not be working correctly. Contact your local
JASCO distributor.

7.2.2 Errors due to High Pressure (OVER PRESS)

The first thing to check is whether the tubing or column is clogged. Remove the tubing or
column from downstream, and then check the operating pressure.
If the instrument is normal but the operating pressure is above the normal range, increase the
maximum pressure (P. MAX) setting.
If the instrument is abnormal and the operating pressure is above the normal range, see
Section 7.3.2 for how to deal with the error.

7.2.3 Errors due to Exceedingly Low Pressure (UNDER PRESS)

First make sure that the tubing is connected normally and check if solvent leaks from the
connecting ports.
If the instrument is normal but the operating pressure is below the normal range, decrease the
minimum pressure (P. MIN) setting.
If the instrument is abnormal and the operating pressure is below the normal range, see
Section 7.3.3 for how to deal with the error.

Note: The “UNDER PRESS” message appears when the operating pressure has remained
below the minimum pressure setting for two minutes. Momentary pressure drops
caused by air bubbles drawn into the pump are ignored.

77
7.2.4 ALARM / DG#1 PUMP DOWN
This message is displayed when the vacuum pressure in the degassing unit does not reach
the specified value. If decompression is not enough, the solvents cannot be degassed
sufficiently.
If this message appears, turn the power OFF and ON. If it appears again, the chamber or
vacuum pump may be damaged. Contact your local JASCO distributor.

7.3 Troubleshooting
7.3.1 Abnormal Operating Pressure
The instrument continuously monitors the operating pressure. Such monitoring helps detect
pump problems, and clogged tubing or line filters. JASCO recommends closely monitoring the
operating pressure and the pressure variation under normal operating conditions.
This section described likely causes for problems that might occur.

Check Valve Assembly (OUT) Check Valve Assembly (OUT)

Inlet Tubing Purge Valve Knob Inlet Tubing

Inlet Filter Inlet Filter

Outlet Port
(with Line Filter)

Check Valve Assembly (IN) Check Valve Assembly (IN)

Figure 7.1 Pump Parts and Part Names for the PU-4086-Binary

78
 Pump Head
(Modifier Head)

Check Valve
Assembly (OUT)
Inlet Tubing Inlet Tubing
Outlet Port
(with Line Filter)

Pump Head
(Delivery Head)

Check Valve
Assembly (IN)

Figure 7.2 Pump Parts and Part Names for the PU-4185/4285-Binary

79
7.3.2 When the Operating Pressure is Higher than Normal
Pressure
Table 7.2 Dealing When the Operating Pressure is Higher than Normal Pressure
Cause Checking method What to do
Downstream of the pump is Remove devices and parts that Replace the clogged
clogged. may be clogged in the flow part or flush the
For example, a blockage route, and then flow and monitor clogged instrument.
occurring in manual injector, the operating pressure.
autosampler, column filter,
tubing, fittings, or other
sections.
Line filter is clogged. Flow methanol or water without Replace the line filter
anything connected to the outlet (see Sections 8.8 and
port. If the operating pressure is 8.16).
2.0 MPa or higher at the flow
rate of 2.0 mL/min, the line filter
may be clogged.
Pump inside or internal Remove the line filter in the This problem requires
tubing is clogged. outlet port and pump the solvent servicing by trained
flow. If the pressure is 2.0 MPa JASCO service
or higher at the flow rate of 2.0 personnel. Contact
mL/min, the pump inside such as your local JASCO
pulse damper and internal tubing distributor.
may be clogged.

80
7.3.3 When the Operating Pressure is Lower than Normal
Pressure
Low pressure has two causes. Either the pump cannot provide the specified flow rate, or there
is a leak (such as downstream from the pump). Table 7.3 lists potential causes, checking
methods, and methods for solving problems that may occur due to under-pressure.

Table 7.3 Dealing When the Operating Pressure is Lower than Normal Pressure
Cause Checking method What to do
Pump head contains air Monitor the operating Open the purge valve and
bubbles. pressure and check if the remove air bubbles using a
pressure variation is normal. syringe (see Section 8.2).
Inlet filter is clogged (use of Check for clogging. Replace the inlet filter.
buffer solutions often leads If the inlet filter is clogged,
to clogging). solvent is not supplied
correctly to the pump head
and the flow becomes
unstable.
Leaks from the plunger seal Run the pressure ramp and Torque the pump head
holding test, and check the securing screws.
condition of pump head. If the leaking continues,
replace the plunger seal
(see Sections 8.5, 8.10 and
8.13).
Leaks from the inlet tube Tighten the fitting firmly.
fitting
Leaking downstream of the See the manual of the
pump (manual injector, related instrument.
autosampler, column, fitting,
etc.).
Pump head deterioration Run the pressure ramp and Replace the pump head
holding test. parts (see Sections 8.5, 8.6,
8.10, 8.11, 8.13 and 8.14).

81
7.3.4 Large Variation in Operating Pressure
When the operating pressure varies widely, the usual causes are abnormal flow due to air
bubbles being generated in the pump head, air entering the pump head, or malfunction of the
check valve. Try using degassing solvent (or connecting an inline degasser), purging air
bubbles from the pump head by opening the purge valve, or purging air bubbles using a
syringe, and then check if the flow is normal.
Table 7.4 lists potential causes, checking methods, and methods for solving problems that
may occur due to large variation in operating pressure.

Table 7.4 Dealing When the Operating Pressure Varies Widely

Cause Checking method What to do
Pump head contains air Open the purge valve and
bubbles remove the air bubbles
using a syringe (see Section
8.2).
Inlet filter is clogged (use of Check for clogging. Replace the inlet filter if it is
buffer solutions often leads If the inlet filter is clogged, clogged.
to clogging). solvent is not supplied
correctly to the pump head
and flow becomes unstable.
Insufficient degassing of the Degas the solvents.
solvents
Leaks from the inlet tube Tighten the fitting firmly.
fitting
Leaks from the plunger seal Run the pressure ramp and Torque the pump head
holding test, and check the securing screws.
condition of pump head. If leaking continues, replace
the plunger seal (see
Sections 8.5, 8.10 and 8.13).
Pump head deterioration Run the pressure ramp and Replace the pump head
holding test. parts (see Sections 8.5, 8.6,
8.10, 8.11, 8.13, and 8.14).
Abnormal plunger Metal knocking sound can Replace or reassemble the
movement be heard. pump head section (see
Sections 8.5, 8.6, 8.10, 8.11,
8.13, and 8.14).

82
7.4 Baseline Problems
This section describes problems that may occur when a UV detector is used.

7.4.1 High Baseline Noise

Table 7.5 lists potential causes, checking methods, and methods for solving problems that
may occur due to high baseline noise.

Table 7.5 Dealing with High Baseline Noise

Cause Checking method What to do
Variations in pump operating Stop the flow. If the noise If the cause is in the pump,
pressure stops, the cause is in the check the condition of the
pump. If the noise continues, pump head and monitor the
the cause is in the UV operating pressure. Clean
detector. the pump head. Replace
parts if necessary.
Mixing noise (when running As above Add an extra mixer, change
a gradient program). the mixer chamber volume,
or add a pulse damper to the
pump.
Detector cell problems See the manual for the UV
・ Bubbles detector
・ Contamination
・ Leakage
Decrease in the light See the manual for the UV
intensity of the detector. detector
Deterioration of the light
source or mirror.

< Spike-shaped Baseline Noise >

Table 7.6 lists potential causes, checking methods, and methods for solving problems that
may occur due to baseline noise spikes.

Table 7.6 Dealing with Spike-shaped Baseline Noise

Cause Checking method What to do
Bubbles in the cell (In this Stop flowing. If the noise See the manual of the UV
case, spike-shaped noise stops, there are probably air detector
occurs only toward bubbles in the cell.
increasing absorption.)
Electrical noise Ground the instrument.

83
7.4.2 Large Baseline Drift
Table 7.7 lists potential causes, checking methods, and methods for solving problems that
may occur due to large baseline drift.

Table 7.7 Dealing with Large Baseline Drift

Cause Checking method What to do
Insufficient solvent Does the drift level decrease Wait until solvent has been
replacement with time? replaced completely
(Increase the flow rate if
necessary).
Insufficient column As above As above
equilibration
Time variation of eluent Use an eluent that is more
(composition, dissolved gas suitable for the analytical
level, etc.). conditions.
Detector instability (cell See the manual of the UV
bubbles, contamination, detector
leakage, etc.).
Eluent contamination Lengthen the detector Use a higher-grade eluent.
wavelength. If the drift
decreases, there is a
problem with the eluent.
Contamination of pump or Lengthen the detector Clean the contaminated
instrument upstream from wavelength. If the drift device (see Section 8.4).
the pump becomes smaller, there is a
problem with instrument
contamination.
Contamination downstream Remove the device or part Clean (see Section 8.4) or
from the pump (manual that appears to be replace the contaminated
injector, autosampler, contaminated and check the device or part.
column, tubing, fittings) drift.

84
7.4.3 Periodic Baseline Drift
Table 7.8 lists potential causes, checking methods, and methods for solving problems that
may occur due to periodic baseline drift.

Table 7.8 Dealing with Periodic Baseline Drift

Cause Checking method What to do
Variations in ambient Is a breeze from the air Move the detector away
temperature conditioner striking a from breezes and heaters.
detector? If a column is not Use a column oven.
in a column oven, is a
breeze from the air
conditioner striking the
column?
Variation from an insufficient Change the gradient Add a mixing unit, change
mixing of gradient solutions. composition temporarily and the mixing speed or mixing
monitor how that changes chamber volume.
the baseline. Replace the check valve.

7.4.4 Appearance of Ghost Peaks

Table 7.9 lists potential causes, checking methods, and methods for solving problems that
may occur due to ghost peaks.

Table 7.9 Dealing with Ghost Peaks

Cause Checking method What to do
Contamination of pump or Clean in the pump (see
instrument upstream from Section 8.4), or replace the
the pump tubing with the new one.
Eluent contamination Use a higher-grade eluent.
Use a clean-up column.
Contamination in the sample Inject the eluent and check if Cleaning (see Section 8.4).
injection section (manual the ghost peaks appear. Clean any instrument found
injector or autosampler). to be contaminated.

85
7.5 Chromatogram Problems
7.5.1 Lengthening Retention Time
Table 7.10 lists potential causes, checking methods, and methods for dealing with
lengthening retention time.

Table 7.10 Dealing with Lengthening Retention Time

Cause Checking method What to do
Decrease in actual flow rate Check actual flow rate.
Insufficient column Increase the column
equilibration re-equilibration time.
Variation in ambient When a column oven is not Use a column oven to keep
temperature used, is a breeze from the the column temperature
air conditioner striking the constant.
column?
Variation in eluent Prepare the eluent again.
composition

7.5.2 Shortening Retention Time

Table 7.11 lists potential causes, checking methods, and methods for dealing with shortening
retention time.

Table 7.11 Dealing with Shortening Retention Time

Cause Checking method What to do
Column deterioration Check the column
performance under the
conditions used in the
column performance test.
If the column deteriorates,
replace it with the new one.
Variation in ambient Check whether or not the Use a column oven to keep
temperature wind from an air conditioner the column temperature
brows to the column. constant.
Variation in eluent Prepare the eluent again.
composition

7.5.3 Poor Reproducibility in Retention Time

Table 7.12 lists potential causes, checking methods, and methods for dealing with poor
reproducibility in retention time.

86
 Table 7.12 Dealing with Poor Reproducibility in Retention Time
Cause Checking method What to do
Variations in pump flow rate Check the operating Measure the flow rate.
pressure variation or
measure the flow rate.
Use of improper eluent Examine the eluent
conditions.
Variations in ambient Check whether or not the Use a column oven to keep
temperature wind from an air conditioner the column temperature
brows to the column. constant.

7.5.4 Poor Reproducibility for Peak Area or Peak Height

(Reproducibility for Retention Time is Good)
When retention time reproducibility is normal, the pump is probably not the cause. The cause
may be in the sample injection section (manual injector or autosampler), the sample itself may
have deteriorated, or wrong analytical conditions may have been set.

7.5.5 Peak Waveform Problems (Peak Broadening, Peak

Splitting)
Peak waveform indicates the use of an improper mobile phase or solvent dissolved in a
sample, a deteriorated column, a failed fitting (dead volume increasing), or injection of too
much sample.

7.6 Checking Solvent Leaks and Temporary Treatments

for the PU-4086-Binary
This section describes how to isolate and deal with solvent leaks and their temporary
treatments for the PU-4086-Binary. See Chapter 8 for the maintenance.

Note: We recommend a careful daily check for leaks in the pump section, autosampler,
detector, and other system components, even if the operating pressure and
chromatograms appear normal.

7.6.1 Places where Solvent Leaks may Occur for the

PU-4086-Binary
Figure 7.3 shows the places where solvent leaks may occur for the PU-4086-Binary. When
the leaking place is identified, treat the leaked places referring to Section 7.6.2 and the
following sections.

87
 Purge Valve Leaks Outlet Port (Line Filter)
Leaks (Section 7.6.5)
(Section 7.6.4)

Check Valve Assembly Inlet Tubing Fitting Leaks

Leaks (Section 7.6.3) (Section 7.6.6)

Solvent Leaks between the Pump Heads and Head

Mound Flanges or from the Plunger Washing
Mechanism Section (see Section 7.6.2)

Figure 7.3 Places where Solvent Leaks may Occur for the PU-4086-Binary

7.6.2 Solvent Leaks between the Pump Heads and Head Mound
Flanges or from the Plunger Washing Mechanism Sections
for the PU-4086-Binary
If solvent leaks occur between the pump heads and head mound flanges and from the bottom
sections of the head mount flange, plunger seal may be deteriorated. As a temporary
treatment, re-torque the hex bolts securing the pump heads.
Alternately tighten the hex bolts securing the pump heads so that the space between the
pump head and head mount flange is even (see Figure 7.4). If the space is uneven, loosen
the bolts and tighten them more.
When solvent leaks are found after tightening the bolts more or re-torque cannot be made,
replace the plunger seal with the new one (see Section 8.5).
88
 CAUTION
If the plunger washing mechanism is not used, remove the inlet and outlet ports put on the
bottom of the head mount flange. Or leaked solvents cannot be wasted from the pump
section and may be entered inside the instrument.

Tighten the four hex bolts

evenly using an M4 hex key
wrench.

Figure 7.4 Tightening the Pump Head Bolts for the PU-4086-Binary

7.6.3 Check Valve Assembly Leaks for the PU-4086-Binary

When solvent leaks are found from the check valve assembly, follow the procedures
described below to tighten the assembly more (See Figure 7.5). If the leak continues after the
check valve assembly has been tightened, the assembly may need to be replaced (see
Section 8.6).
After checking the pump head securing bolts are tightened completely, tighten the assembly.
When solvent leaks have stopped, loosen the pump head securing bolts completely, and then
tighten them again. When turning the wrench, hold the pump so that it does not move.
Do not use a wrench that has a handle longer than 20 cm, or it may strip the threads of the
check valve assembly.

Note: When using a torque wrench, set the torque to 25 N.m.

89
 Tighten using a 12-mm
wrench.

Figure 7.5 Tightening the Check Valve Assembly for the PU-4086-Binary

7.6.4 Purge Valve Leaks for the PU-4086-Binary

Table 7.13 lists the methods for stopping various types of purge valve leaks.

Table 7.13 Dealing with Purge Valve Leaks

Type of leak What to do
Leak from the drain tubing ・ Firmly tighten the purge valve knob.
when the purge valve is closed. ・ If the leak persists, replace the purge valve needle
(see Section 8.7).
Leak from the base of the ・ Check for drain tubing clogging.
purge valve knob when the ・ If the drain tubing is not clogged, remove the purge
valve is open. valve knob, leaving the needle in place, and tighten
the purge valve guide (see Figure 7.6).

90
 Note: Tightening the purge valve guide without the
needle inserted may cause the packing to be
overly compressed, making reinsertion of the
needle impossible.

・ If the leak persists, replace the purge valve packing

(see Section 8.7).

Step (2)
Tighten using an 8-mm wrench.

Step (1)
Turn to remove the purge
valve knob.

Figure 7.6 Tightening the Purge Valve for the PU-4086-Binary

7.6.5 Outlet Port (Line Filter) Leaks for the PU-4086-Binary

If the outlet port leaks, tighten the filter holder more. If it still leaks, the packing (PEEK) outside
the line filter body (stainless steel) may be damaged or scratched. See Section 8.8 for how to
replace the line filter.

Note: Loosening the tubing connected to the outlet port may also loosen the line filter, so use
a wrench to stop the outlet port turning.

91
 Tighten using a 12-mm wrench.

Figure 7.7 Tightening the Outlet Filter Holder for the PU-4086-Binary

7.6.6 Inlet Tubing Fitting Leaks for the PU-4086-Binary

If the inlet tube fitting leaks, tighten the screw more. If it still leaks, replace the tubing or fitting
and ferrule that are used to the tubing.

92
7.7 Checking Solvent Leaks and Temporary Treatments
for the PU-4185-Binary
This section describes how to isolate and deal with solvent leaks and their temporary
treatments for the PU-4185-Binary. See Chapter 8 for the maintenance.

Note: We recommend a careful daily check for leaks in the pump section, autosampler,
detector, and other system components, even if the operating pressure and
chromatograms appear normal.

7.7.1 Places where Solvent Leaks may Occur for the

PU-4185-Binary
Figure 7.8 shows the places where solvent leaks may occur for the PU-4185-Binary. When
the leaking place is identified, treat the leaked places referring to Section 7.7.2 and the
following sections.

Check Valve Assembly Leaks

(Section 7.7.3)

Purge Valve Leaks

(Section 7.7.4)

Solvent Leaks between the Pump

Heads and Head Mound Flanges
Inlet Tubing Fitting Leaks
or from the Plunger Washing
(Section 7.7.5)
Mechanism Section (Section 7.7.2)

Figure 7.8 Places where Solvent Leaks may Occur for the PU-4185-Binary

93
7.7.2 Solvent Leaks between the Pump Heads and Head Mound
Flanges or from the Plunger Washing Mechanism Sections
for the PU-4185-Binary
If solvent leaks occur between the pump heads and head mound flanges and from the bottom
sections of the head mount flange, plunger seal may be deteriorated. As a temporary
treatment, tighten the hex bolts more securing the pump heads. When tightening the pump
heads more for the PU-4185-Binary, perform after removing the purge valve as shown in
Figure 7.9.
Alternately tighten the hex bolts securing the pump heads so that the space between the
pump head and head mount flange is even (see Figure 7.9). If the space is uneven, loosen
the bolts and re-tighten them.
When solvent leaks are found after tightening the bolts more or re-torque cannot be made,
replace the plunger seal with the new one (see Section 8.10).

CAUTION
If the plunger washing mechanism is not used, remove the inlet and outlet ports put on the
bottom of the head mount flange. Or leaked solvents cannot be wasted from the pump
section and may be entered inside the instrument.

Evenly tighten the

four hex bolts using
an M4 hex key
wrench.

Remove the purge valve knob on the

modifier head.

Figure 7.9 Tightening the Pump Head Bolts for the PU-4185-Binary

94
7.7.3 Check Valve Assembly Leaks for the PU-4185-Binary
When solvent leaks are found from the check valve assembly, follow the procedures
described below to tighten the assembly more (see Figure 7.10). If the leak continues after the
check valve assembly has been tightened, the assembly may need to be replaced (see
Section 8.11).
After checking the pump head securing bolts are tightened completely, tighten the assembly.
When solvent leaks have stopped, loosen the pump head securing bolts completely, and then
tighten them again. When turning the wrench, hold the pump so that it does not move.
Do not use a wrench that has a handle longer than 20 cm, or it may strip the threads of the
check valve assembly.

Note: When using a torque wrench, set the torque to 25 N.m.

Tighten using a 12-mm

wrench.

Figure 7.10 Tightening the Check Valve Assembly for the PU-4185-Binary

95
7.7.4 Purge Valve Leaks for the PU-4185-Binary
Table 7.14 lists the methods for stopping various types of purge valve leaks.

Table 7.14 Dealing with Purge Valve Leaks

Type of leak What to do
Leak from the drain tubing ・ Firmly tighten the purge valve knob.
when the purge valve is closed. ・ If the leak persists, replace the purge valve needle
(see Section 8.12).
Leak from the base of the ・ Check for drain tubing clogging.
purge valve knob when the ・ If the drain tubing is not clogged, remove the purge
valve is open. valve knob, leaving the needle in place, and tighten
the purge valve guide (see Figure 7.11).
Note: Tightening the purge valve guide without the
needle inserted may cause the packing to be
overly compressed, making reinsertion of the
needle impossible.

・ If the leak persists, replace the purge valve packing

(see Section 8.12).

Step (2)
Tighten using an 8-mm
wrench.

Step (1)
Turn to remove the purge
valve knob.

Figure 7.11 Tightening the Purge Valve for the PU-4185-Binary

96
7.7.5 Inlet Tubing Fitting Leaks for the PU-4185-Binary
If the inlet tube fitting leaks, tighten the screw more. If it still leaks, replace the tubing or fitting
and ferrule that are used to the tubing.

7.8 Checking Solvent Leaks and Temporary Treatments

for the PU-4285-Binary
This section describes how to isolate and deal with solvent leaks and their temporary
treatments for the PU-4285-Binary. See Chapter 8 for the maintenance.
Note: We recommend a careful daily check for leaks in the pump section, autosampler,
detector, and other system components, even if the operating pressure and
chromatograms appear normal.
7.8.1 Places where Solvent Leaks may Occur for the
PU-4285-Binary
Figure 7.12 shows the places where solvent leaks may occur for the PU-4285-Binary. When
the leaking place is identified, treat the leaked places referring to Section 7.8.2 and the
following sections.

Check Valve Assembly Leaks

(Section 7.8.3)

Purge Valve Leaks

(Section 7.8.4)

Solvent Leaks between the Pump

Heads and Head Mound Flanges
Inlet Tubing Fitting Leaks
or from the Plunger Washing
(Section 7.8.5)
Mechanism Section (Section 7.8.2)

Figure 7.12 Places where Solvent Leaks may Occur for the PU-4285-Binary

97
7.8.2 Solvent Leaks between the Pump Heads and Head Mound
Flanges or from the Plunger Washing Mechanism Sections
for the PU-4285-Binary
If solvent leaks occur between the pump heads and head mound flanges and from the bottom
sections of the head mount flange, plunger seal may be deteriorated. As a temporary
treatment, tighten the hex bolts more securing the pump heads. When tightening the pump
heads more for the PU-4285-Binary, perform after removing the purge valve as shown in
Figure 7.13.
Alternately tighten the hex bolts securing the pump heads so that the space between the
pump head and head mount flange is even (see Figure 7.13). If the space is uneven, loosen
the bolts and re-tighten them.
When solvent leaks are found after tightening the bolts more or re-torque cannot be made,
replace the plunger seal with the new one (see Section 8.13).

CAUTION
If the plunger washing mechanism is not used, remove the inlet and outlet ports put on the
bottom of the head mount flange. Or leaked solvents cannot be wasted from the pump
section and may be entered inside the instrument.

Evenly tighten the

four hex bolts using
an M4 hex key
wrench.

Remove the purge valve knob on the

modifier head.

Figure 7.13 Tightening the Pump Head Bolts for the PU-4285-Binary

98
7.8.3 Check Valve Assembly Leaks for the PU-4285-Binary
When solvent leaks are found from the check valve assembly, follow the procedures
described below to tighten the assembly more (see Figure 7.14). If the leak continues after the
check valve assembly has been tightened, the assembly may need to be replaced (see
Section 8.14).
After checking the pump head securing bolts are tightened completely, tighten the assembly.
When solvent leaks have stopped, loosen the pump head securing bolts completely, and then
tighten them again. When turning the wrench, hold the pump so that it does not move.
Do not use a wrench that has a handle longer than 20 cm, or it may strip the threads of the
check valve assembly.

Note: When using a torque wrench, set the torque to 25 N.m.

Tighten using a 12-mm

wrench.

Figure 7.14 Tightening the Check Valve Assembly for the PU-4285-Binary

99
7.8.4 Purge Valve Leaks for the PU-4285-Binary
Table 7.15 lists the methods for stopping various types of purge valve leaks.

Table 7.15 Dealing with Purge Valve Leaks

Type of leak What to do
Leak from the drain tubing ・ Firmly tighten the purge valve knob.
when the purge valve is closed. ・ If the leak persists, replace the purge valve needle
(see Section 8.15).
Leak from the base of the ・ Check for drain tubing clogging.
purge valve knob when the ・ If the drain tubing is not clogged, remove the purge
valve is open. valve knob, leaving the needle in place, and tighten
the purge valve guide (see Figure 7.15).
Note: Tightening the purge valve guide without the
needle inserted may cause the packing to be
overly compressed, making reinsertion of the
needle impossible.

・ If the leak persists, replace the purge valve packing

(see Section 8.15).

Step (2)
Tighten using an 8-mm
wrench.

Step (1)
Turn to remove the purge
valve knob.

Figure 7.15 Tightening the Purge Valve for the PU-4285-Binary

100
7.8.5 Inlet Tubing Fitting Leaks for the PU-4285-Binary
If the inlet tube fitting leaks, tighten the screw more. If it still leaks, replace the tubing or fitting
and ferrule that are used to the tubing.

7.9 Leaving the Instrument Idle for Extended Periods

Organic solvents, water, buffers or solvents with halogen groups will damage parts if left
standing in the instrument for three days or more. To prevent corrosion, replace them with a
safe solvent such as Methanol or Acetonitrile before leaving the unit idle.

CAUTION
If the solvent is not replaced, salt crystals or mold may form and damage the instrument.

101
8. Maintenance
This chapter describes how to replace consumable parts such as the plunger seal and how to
do routine maintenance such as replacing solvent.

CAUTION
(1) Use only standard parts approved by JASCO, as described in the following sections.
If non-approved parts are used, this may cause other parts of the instrument to break
or trouble may occur.
(2) Always turn the power OFF and unplug the power cable before starting maintenance
unless otherwise directed.

8.1 Regular Maintenance

Maintenance should be done at suitable intervals for trouble free use. If the instrument is
used heavily (beyond a 40-hour week), maintain more frequently.

8.2 Air Bubble Removal

If air bubbles enter or are formed in the pump head, the pump cannot work properly and
increase of pulse flow, flow rate drop, or baseline variation may result. To solve them, remove
air bubbles form the pump head.
Before the air bubble removal, open the purge valve and check if solvents go out from the
drain tubing when the pump is flowed at the flow rate of 1 to 2 mL/min.
Check for blockage in the purge valve and the drain tubing, and remove air bubbles according
to the following methods.

(1) Air bubble removal by fast flow

Set the flow rate to the maximum flow rate and the maximum pressure to 1.0 MPa. Leave
the purge valve open and repeatedly start and stop flow.

(2) Air Bubble removal by syringe

If Method (1) cannot sufficiently remove air bubbles, attach a syringe to the drain tubing
and draw out the solvents. If this is difficult, attach the syringe directly to the pump head
and draw out the solvents.
Sometimes there are some resistances when drawing by a syringe and it is difficult to
draw. In this case, the inlet filter or inside the pump may be clogged. See Sections 8.8
and 8.16 for checking the inlet filter clogging.

102
8.3 Solvent Replacement
This section explains how to replace the solvent quickly.

8.3.1 General Considerations for Solvent Replacement

Note: The solvent in the instrument shipped from the factory is methanol.

Be sure to replace the solvent being replaced in the inline degasser, pump head, and tubing
for the solvent that is inter-miscible with the former at any composition. Figure 8.1 shows the
compatibility of solvents used frequently for liquid chromatography.

Aqueous Solvents

Buffer
Salt solvents

Water

Ethanol
Organic IPA
Solvents (Acetone)

Acetonitrile
Methanol

Chloroform
Ethyl acetate

n-Hexane
Isooctane

Figure 8.1 Compatibility of Solvents

103
 CAUTION
If the solvents replaced are not inter-miscible with the new solvents in any composition,
salts may precipitate in the instrument, which may cause the instrument to malfunction,
shorten the service life, or deteriorate the performance.

8.3.2 Solvent Replacement

To replace solvent quickly, stop the flow and use a syringe to draw out solvents from the drain
port as described in Section 8.2 - (2). This operation replaces solvents in the parts from the
inlet tubing to the purge valve.
However, the solvents are not replaced in the pump such as in the pressure sensor and outlet
port, which are placed downstream of the purge valve. Therefore, to replace solvents in these
parts, close the purge valve and increase the flow rate.
Replace the solvents according to the following procedures.

(1) Connect an appropriate length of 1/16-inch OD stainless steel or PEEK tubing to the
outlet port and route another end of the tubing to the waste bottle.

(2) Set the flow rate to the maximum, and the maximum pressure to 3.0 MPa. Depending on
the size (ID and length) of the tubing connected to the pump outlet port and the solvent
viscosity, “OVER PRESS” message may appear and the flow may stop. In this case,
increase the maximum pressure setting.

(3) Prepare the new solvent.

(4) Open the purge valve to replace the solvent.

(5) Transfer the inlet tubing form the container holding the solvent to a container holding a
new solvent and start the flow.

(6) While continuing to flow, lift the inlet tubing out of the solvent, take in a few cm of air
(bubble), and reinsert the inlet tubing into the solvent.

(7) Air bubble will be taken up into the pump and discharged from the drain tubing. Check
that the solvent continues to flow out from the drain tubing without interruption. If the
solvent flow stops or if air bubbles remain in the inlet tubing, attach a syringe to the drain
tubing and draw out the solvents.

(8) The solvent has been replaced into the purge valve. Next, replace the solvent inside the
pump.

104
(9) Close the purge valve. Check that the solvent continues to flow out of the tubing
connected to the outlet port without interruption. Pump at the maximum flow rate for about
3 to 5 minutes.

(10) The solvent has been replaced in the pump.

8.4 Cleaning inside the Pump

Clean inside the pump when a problem has been traced to the pump head deterioration or
contamination inside the pump. Such problems are listed below.

・ Large pressure variation (see Section 7.3.4)

・ High baseline noise (see Section 7.4.1)
・ Large drift (see Section 7.4.2)
・ Appearance of ghost peaks (see Section 7.4.4)

The procedures for cleaning are as follows.

(1) First, prime the pump with water (HPLC-grade). Set the flow rate to 3 to 5 mL/min, and
flow for 15 to 20 minutes (about 100 mL) to replace inside the pump with water
completely.

(2) Next, replace with organic solvent such as methanol. Set the flow rate to 3 to 5 mL/min,
and flow for 15 to 20 minutes (about 100 mL).

(3) Repeat Steps (1) and (2) if more cleaning is necessary.

(4) Replace the solvent intermisible with the solvent used in the analysis.

8.5 Replacing Plunger Seal for the PU-4086-Binary

The plunger seal in the pump head stops solvent leaking as the plunger moves backward and
forward. When the plunger seal deteriorates, leaks begin and may lead to flow rate drop,
decrease of reproducibility due to unstable flow, or baseline variation.
JASCO recommends replacing the plunger seal at regular intervals, depending on the
frequency of instrument use and the kind of solvent being used. Also recommends replacing
the plunger seals in both pump heads at the same time.
Replace the solvent inside the pump with methanol or water (HPLC-grade) when replacing
the plunger seal.

105
 CAUTION
Strictly obey the following instructions when replacing the plunger seal.

(1) Replace the solvent inside the pump with methanol or water (HPLC-grade) when
replacing the plunger seal.
(2) Disassembling the pump parts is required to replace the plunger seal. At this time,
solvents remaining in the pump may go out and they may stick in your fingers. Use
chemical groves to prevent putting solvents to you.
(3) Sometimes solvents may leak during the pressure ramp and holding test. In this case,
wipe out the solvents quickly using tissue. The tissue used for wiping should be
wasted after suitable treatments.
(4) Salts in buffer solutions may form crystals, causing poor instrument performance, so
do not use buffers for the pressure ramp and holding test.
(5) Note that the power is turned OFF, unless otherwise stated.

Tools required M4 hex key wrench (subtense: 3-mm)

8-mm wrench
Small flat-blade screwdriver
(precise flat-blade screwdriver)
Plunger seal pre-forming tool
Part Number
Parts required Plunger seal (2 pcs./pk) 7004-H108A

Removing the pump heads

(1) Disconnect all the tubing from the pump

heads.

106
(2) Use an M4 hex key wrench to loosen the
four hex bolts on the pump head evenly
and alternately.

Remove the pump heads. Slowly pull out Delivery

the pump heads straight in line with the Head
plunger. If they are pulled out at an angle,
the plunger may break.
(1) (2)
In addition, remove each delivery head
separately.

Delivery
Head

Removing the plunger seal

(3) Using a small flat-blade screwdriver

(precise screwdriver), remove the
plunger seal and back-up ring (made by
PEEK).
Be careful not to scratch the inside
surface of the seal attachment hole when
you press the plunger seal with the
Plunger
screwdriver.
Seal
If the inside surface of the seal
シール
attachment hole is scratched, it causes
solvent leaks.

Back-up Ring
(PEEK)
(4) If any plunger seal and backup ring
Remove particles remain in
particles remain in the pump heads, flush
the pump head.
inside the pump head with Methanol
completely, and then wipe using a
chemical tissue.
Be careful not to scratch the inside
surface of the seal attachment hole.

107
(5) Dirt on the plunger ages the plunger seal. Remove using a chemical tissue which
mehanol is put. If particles cannot be removed easily, place some non-abrasive
household cleaner on a cloth and rub the plungers clean.

Installing the new plunger seal

(6) Press a new, unformed plunger Carefully press the plunger seal so
seal into the seal pre-forming that the edge is not damaged.
tool. Wait for a few minutes.
When pressing a new seal into
the seal pre-forming tool, care-
Press the plunger
fully press it straight in gently.
seal towards the
Note that the edge of the Plunger Seal
curving surface
plunger seal is easily Pre-forming Tool
side.
damaged.

Press on the push-up pole to

extract the seal, and install it
immediately.

(7) Install the new plunger seal to

the pump head. As the figure
shows, slowly press it straight
Plunger seal
in with one finger.
Be sure to press straight in
gently by your finger (not use
any tool), because the edge of
the plunger seal is easily
Pump head
damaged.
Moistening the plunger seal
and the pump head seal hole
with methanol often makes
insertion easier.

108
(8) Insert the back-up ring.
In this time, get the ring wet
with a solvent (such as
methanol) to avoid falling the
ring from the head.

Installing the pump heads

(9) Install the pump heads separately.

The dotted mark is on the pump head.
Install the pump head so that this mark is The dotted mark is
upside. on the pump head.
When installing the pump heads, gently Install the pump
insert the pump head straight in line with head so that this
the plunger and guide pins. If it is not mark is upside.
inserted straight, the plunger may break
or the plunger seal may be damaged,
and it may cause solvent leak.

Delivery
Head
ヘッド
(10) Use an M4 hex key wrench to tighten the
four hex bolts. Use a crisscross pattern to tighten the

Use a crisscross pattern to tighten the four hex bolts evenly.

four hex bolts evenly.

Be careful to tighten the bolts evenly
(crisscross pattern) and firmly.

109
(11) Connect all the tubing.

Check after replacing the plunger seal

(12) When the replacement has been completed, start the flow and check if solvents leak
from the plunger seal.

(13) Reset the information on the plunger seal replacement and the number of plunger
driving if necessary. See Section 6.5.1 for the setting.

110
8.6 Replacing Check Valve Assembly for the
PU-4086-Binary

Tools required 8-mm wrench

12-mm wrench
(Torque wrench (with 12-mm socket))
Part Number
Parts required Check valve assembly (IN) 6965-H104A
Check valve assembly (OUT) 6965-H105A

CAUTION
Strictly obey the following instructions when replacing the check valve assembly.

(1) Replace the solvent inside the pump with methanol or water (HPLC-grade) when
replacing the check valve assembly.
(2) Note that the pump is stopped and is not pressurized. If pressurized, solvents may go
out and scatter.
(3) Disassembling the pump parts is required to replace the check valve assembly. At this
time, solvents remaining in the pump may go out and they may stick in your fingers.
Use chemical groves to prevent putting solvents to you.
(4) Sometimes solvents may leak during the pressure ramp and holding test. In this case,
wipe out the solvents quickly using tissue. The tissue used for wiping should be
wasted after suitable treatments.
(5) Note that the power is turned OFF, unless otherwise stated.

(1) Disconnect all the tubing and inlet tubing

from the pump heads.

111
(2) Use a 12-mm wrench or torque wrench
(with 12-mm socket) to remove the check Check Valve Assembly (OUT)
valve.

Check Valve Assembly (IN)

Remember the following when removing and installing the check valve assembly.
1) Tighten the bolts securing the pump head before you loosen the check valve
assembly, or the plunger may break.
2) Loosen the pump-head bolts to their original tightness after the check valve
assembly has been replaced.
3) When turning the wrench, hold the pump steady.
4) The check valve assembly has an IN section and an OUT section. Note that the
direction.
5) When tightening the check valve assembly, be careful not to touch the wrench
with the plunger washing mechanism. If the wrench is turned with toughing to the
plunger washing mechanism, it may be damaged.

If replacing the check valve assembly is

difficult while the pump head is attached,
remove the pump head first (see Section
8.5).
While the pump head is being removed,
make sure it is held securely with an
adjustable wrench (see the figure in the
right) or in a vise.

112
(3) Check if there are any remaining gasket
materials or other contaminants from the
inside surface of the check valve
Check if there are
assembly. If there are, clean them by
any remaining gasket
rinsing the area with methanol in a
materials or other
syringe. And then wipe the inside surface
contaminants.
with a piece of clean cloth or chemical
tissue.

Installing the new check valve assembly

(4) Using a 12-mm wrench, install the check

valve assembly with moistening with
methanol.

If a torque wrench is available, set the Check Valve

torque to 25 N.m, and then tighten the Assembly (OUT)
check valve.

Check Valve
Assembly (IN)

(5) Connect all the tubing.

Check after replacing the check valve assembly

(6) When the replacement has been completed, start the flow and check if solvents leak
from the check valve assembly.

113
8.7 Replacing Purge Valve Needle and Purge Valve
Packing for the PU-4086-Binary
When solvent leaks are found from the purge valve section, replace the purge valve needle or
purge valve packing.

Tools required M2 hex key wrench (subtense 1.5-mm)

8-mm wrench
Part Number
Parts required Purge valve needle 7002-0403A
Purge valve packing (2 pcs./pk) 6696-H305A

CAUTION
Strictly obey the following instructions when replacing the purge valve section.

(1) Salts in buffer solutions may form crystals, causing poor instrument performance, so
do not use buffers for the pressure ramp and holding test.
(2) Note that the pump is stopped. Solvent will leak if tubing is removed while the pump is
turned ON.
(3) Note that the power is turned OFF, unless otherwise stated.
(4) Before removing the purge valve needle, remove the inlet tubing or put the solvent
container below the level of the pump if necessary.

Replacing the purge valve needle

(1) Turn the purge valve knob counter-

clockwise to remove the purge valve.

Purge Valve

114
(2) Use an M2 hex key wrench (subtense:
1.5-mm) to remove the drain valve (2)
needle from the knob.
Needle

Knob (1)

(3) Use an M2 hex key wrench to install the new drain valve needle to the knob by the
reversed procedures in Step (2).

(4) Use a hex key wrench to fix the new needle to the knob.

(5) Install the purge valve to the outlet port body.

(6) When the replacement has been completed, start the flow and check if solvents leak
from the purge valve section.

Replacing the purge valve packing

(1) Turn the purge valve knob counter-

clockwise to remove the purge valve.

Purge Valve

115
(2) Using an 8-mm wrench, turn to remove
the needle guide counterclockwise.

Needle Guide

(3) Remove the packing from the guide, and

then install the new packing to the guide. Packing

Needle Guide

(4) Tighten the needle guide screw lightly

with your fingers.

Needle Guide

(5) Fix the purge valve to needle guide by

making approximately two turn.
Check if there are any spaces between
the purge valve and outlet port body.

Purge Valve

116
(6) Insert an 8-mm wrench to the spaces
between the outlet port body and the
purge valve, and tighten the needle (1)
guide.
And then turn to firmly fix the purge valve.

(2)

(7) When the replacement has been completed, run the solvent leak test, and check if
solvents leak from the purge valve section.

117
8.8 Replacing Line Filter for the PU-4086-Binary
Check the operating pressure without connecting any tubing to the pump outlet and by flowing
at the flow rate of 2.0 mL/min. If the pressure is more than 2 MPa, the line filter may be
clogged. In this case, replace the line filter.

Tools required 8-mm wrench

Small flat-blade screwdriver
(Precise flat-blade screwdriver)
Part Number
Parts required Line filter kit (2 pcs./pk) 7004-H301A
Filter retainer screw 6688-0602C
(if necessary)

(1) Use a 12-mm wrench to remove the filter

retainer screw.

Filter
Retainer
Screw

(2) If there are any gasket materials or dirt in

the filter holder, remove them.

Filter Retainer Screw

Check if there are any

gasket materials or dirt.

(3) Remove the line filter (with packing) from Line Filter
the filter retainer screw.
Use a small flat-blade screwdriver to
remove the filter from the edge.

Filter Retainer Screw

118
 (4) Install the new line filter to the filter
retainer screw. Line Filter

Filter Retainer Screw

(5) Use a 12-mm wrench to fix the filter

retainer screw to the pump outlet.

Filter
Retainer
Screw

(6) When the replacement has been completed, start the flow and check if solvents leak
from the filter retainer screw.

8.9 Replacing Magnetic Stirrer and Filter in the Mixing

Chamber for the PU-4086-Binary
Stirrer bar is in the mixing chamber. It rotates at the speed of 1000 to 5100 rpm. Due to this
higher rotation, the surface of the stirrer bar will be worn when it is used for a long time. We
recommend checking the condition of the stirrer bar once a month. If the wearing is found,
replace with the new one.
Also, if solvents leaks from the filter retainer screw on the mixing chamber or if the operating
pressure is increased by clogging the filter, replace it with the new one.

Tools required 8-mm wrench

Phillips screwdriver
Part Number
Parts required Stirrer bar (3 pcs./pk) 6783-H111A
Outlet filter assembly for the 7007-H342A
mixing chamber

119
 CAUTION
Strictly obey the following instructions when replacing the stirrer bar and filter in the mixing
chamber.

(1) Note that the power is turned OFF, unless otherwise stated.
(2) Replace the solvent inside the mixer chamber with methanol or water (HPLC-grade)
when replacing the stirrer bar and filter.
(3) Disassembling the chamber is required to replace the stirrer bar and filter. At this time,
solvents remaining in the chamber may go out and they may stick in your fingers. Use
chemical groves to prevent putting solvents to you.
(4) Sometimes solvents may leak during the pressure ramp and holding test. In this case,
wipe out the solvents quickly using tissue. The tissue used for wiping should be
wasted after suitable treatments.
(5) Salts in buffer solutions may form crystals, causing poor instrument performance, so
do not use buffers for the pressure ramp and holding test.

Replacing the stirrer bar

(1) Remove the mixing chamber from the

pump.

120
(2) Loosen the filter retainer screw with
holding the mixing chamber.

(3) Remove the filter retainer screw.

(4) Take out the old stirrer bar inside the

chamber and then replace with the new
one.

121
(5) Tighten the filter retainer screw.

(6) Check if solvents leaks from the mixing

chamber.
If not, install the unit to the pump.

Replacing the filter

(1) Remove the mixing chamber from the

pump.

122
(2) Loosen the filter retainer screw with
holding the mixing chamber.

(3) Remove the filter retainer screw.

(4) Remove the filter assembly from the filter

retainer screw and then replace with the
new one.

123
(5) Tighten the filter retainer screw.

(6) Check if solvents leaks from the mixing

chamber.
If not, install the unit to the pump.

8.10 Replacing Plunger Seal for the PU-4185-Binary

The plunger seal in the pump head stops solvent leaking as the plunger moves backward and
forward. When the plunger seal deteriorates, leaks begin and may lead to flow rate drop,
decrease of reproducibility due to unstable flow, or baseline variation.
JASCO recommends replacing the plunger seal at regular intervals, depending on the
frequency of instrument use and the kind of solvent being used. Also recommends replacing
the plunger seals in both pump heads at the same time.
Replace the solvent inside the pump with methanol or water (HPLC-grade) when replacing
the plunger seal.

124
 CAUTION
Strictly obey the following instructions when replacing the plunger seal.

(1) Replace the solvent inside the pump with methanol or water (HPLC-grade) when
replacing the plunger seal.
(2) To replace the plunger seal, disassemble the pump parts. At this time, solvents
remaining in the pump may go out and they may stick in your fingers. Use chemical
groves to prevent putting solvents to you.
(3) Sometimes solvents may leak during the pressure ramp and holding test. In this case,
wipe out the solvents quickly using tissue. The tissue used for wiping should be
wasted after suitable treatments.
(4) Salts in buffer solutions may form crystals, causing poor instrument performance, so
do not use buffers for the pressure ramp and holding test.
(5) Note that the power is turned OFF, unless otherwise stated.

Tools required M4 hex key wrench (subtense: 3-mm)

8-mm wrench
Small flat-blade screwdriver
(precise flat-blade screwdriver)
Plunger seal pre-forming tool
Part Number
Parts required Plunger seal (2 pcs./pk) 7003-H301A

Removing the pump head

(1) Disconnect all the tubing from the pump

heads.

125
(2) Turn the purge valve knob counter-
clockwise to remove the purge valve.

(1)
(2)

Purge Valve

(3) Use an M4 hex key wrench (subtense:

3-mm) to loosen the four hex bolts on
(3)
the pump head evenly and alternately. When the back-up rings
(made of PEEK) remain in
the plungers, remove
Remove the pump heads. Slowly pull them.
out the pump heads straight in line with
the plunger. If they are pulled out at an
angle, the plunger may break. Delivery Head

The pump head has a delivery head (2)

and a modifier head. Remove each
head separately.
Modifier Head
When the back-up rings (made of (1)
PEEK) remain in the plungers, remove
them.

126
Removing the plunger seal

(4) Using a small flat-blade screwdriver

(precise screwdriver), remove the
plunger seal and back-up ring (made by
PEEK).
Be careful not to scratch the inside
surface of the seal attachment hole
when you press the plunger seal with
the screwdriver.
If the inside surface of the seal
Plunger
attachment hole is scratched, it causes
Seal
solvent leaks. Back-up Ring

(5) If any plunger seal and backup ring

Remove particles remain in
particles remain in the pump heads,
the pump head.
flush inside the pump head with
Methanol completely, and then wipe
using a chemical tissue.
Be careful not to scratch the inside
surface of the seal attachment hole.

(6) Dirt on the plunger ages the plunger seal. Remove using a chemical tissue which
mehanol is put. If particles cannot be removed easily, place some non-abrasive
household cleaner on a cloth and rub the plungers clean.

Installing the new plunger seal

(7) Press a new, unformed plunger Carefully press the plunger seal so
seal into the seal pre-forming that the edge is not damaged.
tool. Wait for a few minutes.
When pressing a new seal into
the seal pre-forming tool, care-
Press the plunger
fully press it straight in gently.
seal towards the
Note that the edge of the Plunger Seal
curving surface
plunger seal is easily Pre-forming Tool
side.
damaged.

127
 Press on the push-up pole to
extract the seal, and install it
immediately.

(8) Install the new plunger seal to

the pump head. As the figure Plunger Seal
shows, slowly press it straight
in with one finger.
Be sure to press straight in
gently by your finger (not use
any tool), because the edge of
the plunger seal is easily
damaged.
Moistening the plunger seal
and the pump head seal hole
with methanol often makes
insertion easier.

(9) Next, put the backup ring in the

pump head. When installing
the ring, slowly press it straight
in with one finger.
Moistening the backup ring
with methanol helps to attach
firmly to the seal.

Back-up Ring

128
Installing the pump heads

(10) Install the pump heads separately after

checking the back-up ring is attached into
Check the back-up
the pump head.
ring (made of PEEK)
When installing the pump heads, gently
is attached into the
insert the pump head straight in line with
pump head.
the plunger and guide pins. If it is not
inserted straight, the plunger may break
or the plunger seal may be damaged,
Delivery
and it may cause solvent leak.
Head

Modifier Head

(11) Use an M4 hex key wrench to tighten the

four hex bolts. Use a crisscross pattern to tighten the
Use a crisscross pattern to tighten the four hex bolts evenly.
four hex bolts evenly.
Be careful to tighten the bolts evenly
(crisscross pattern) and firmly.

129
(12) Install the purge valve.

Turn the purge valve

knob clockwise to
install the purge
valve.

(13) Connect all the tubing.

Checking after replacing the plunger seal

(14) When the replacement has been completed, start the flow and check if solvents leak
from the plunger seal.

(15) Reset the information on the plunger seal replacement and the number of plunger
driving if necessary. See Section 6.5.1 for the setting.

130
8.11 Replacing Check Valve Assembly for the
PU-4185-Binary

Tools required 8-mm wrench

12-mm wrench
(Torque wrench (with 12-mm socket))
Part Number
Parts required Check valve assembly (IN) 6949-H104A
Check valve assembly (OUT) 6949-H105A

CAUTION
Strictly obey the following instructions when replacing the check valve assembly.

(1) Replace the solvent inside the pump with methanol or water (HPLC-grade) when
replacing the check valve assembly.
(2) Note that the pump is stopped and is not pressurized. If pressurized, solvents may go
out and scatter.
(3) Disassembling the pump parts is required to replace the check valve assembly. At this
time, solvents remaining in the pump may go out and they may stick in your fingers.
Use chemical groves to prevent putting solvents to you.
(4) Sometimes solvents may leak during the pressure ramp and holding test. In this case,
wipe out the solvents quickly using tissue. The tissue used for wiping should be
wasted after suitable treatments.
(5) Note that the power is turned OFF, unless otherwise stated.

131
(1) Disconnect all the tubing and inlet tubing
from the pump heads.

(2) Use a 12-mm wrench or torque wrench

(with 12-mm socket) to remove the check
valve.

Check Valve
Assembly (OUT)

Check Valve
Assembly (IN)

Remember the following when removing and installing the check valve assembly.
1) Tighten the bolts securing the pump head before you loosen the check valve
assembly, or the plunger may break.
2) Loosen the pump-head bolts to their original tightness after the check valve
assembly has been replaced.
3) When turning the wrench, hold the pump steady.

132
 4) The check valve assembly has an IN section and an OUT section. Note that the
direction.
5) When tightening the check valve assembly, be careful not to touch the wrench
with the plunger washing mechanism. If the wrench is turned with toughing to the
plunger washing mechanism, it may be damaged.

If replacing the check valve assembly is

difficult while the pump head is attached,
remove the pump head first (see Section
8.10).
While the pump head is being removed,
make sure it is held securely with an
adjustable wrench (see the figure in the
right) or in a vise.

(3) Check if there are any remaining gasket

materials or other contaminants from the
inside surface of the check valve
Check if there are
assembly. If there are, clean them by
any remaining gasket
rinsing the area with methanol in a
materials or other
syringe. And then wipe the inside surface
contaminants.
with a piece of clean cloth or chemical
tissue.

Installing the new check valve assembly

(4) Using a 12-mm wrench, install the check

valve assembly with moistening with
methanol.

If a torque wrench is available, set the

torque to 25 N.m, and then tighten the
check valve.
Check Valve
Assembly (OUT)

Check Valve
Assembly (IN)

133
 (5) Connect all the tubing.

Check after replacing the check valve assembly

(6) When the replacement has been completed, start the flow and check if solvents leak
from the check valve assembly.

8.12 Replacing Purge Valve Needle and Purge Valve

Packing for the PU-4185-Binary
When solvent leaks are found from the purge valve section, replace the purge valve needle or
purge valve packing.

Tools required M2 hex key wrench (subtense: 1.5-mm)

8-mm wrench
Part Number
Parts required Purge valve needle 7002-0403A
Purge valve packing (2 pcs./pk) 6696-H305A

134
 CAUTION
Strictly obey the following instructions when replacing the purge valve section.

(1) Salts in buffer solutions may form crystals, causing poor instrument performance, so
do not use buffers for the pressure ramp and holding test.
(2) Note that the pump is stopped. Solvent will leak if tubing is removed while the pump is
turned ON.
(3) Note that the power is turned OFF, unless otherwise stated.
(4) Before removing the purge valve needle, remove the inlet tubing or put the solvent
container below the level of the pump if necessary.

Replacing the purge valve needle

(1) Turn the purge valve knob counter-

clockwise to remove the purge valve.

パージバルブ

(2) Use an M2 hex key wrench (subtense:

1.5-mm) to remove the drain valve (2)
needle from the knob.
Needle

Knob (1)

(3) Use an M2 hex key wrench to install the new drain valve needle to the knob by the
reversed procedures in Step (2).

135
(4) Use a hex key wrench to fix the new needle to the knob.

(5) Install the purge valve to the pump.

(6) When the replacement has been completed, start the flow and check if solvents leak
from the purge valve section.

Replacing the purge valve packing

(1) Turn the purge valve knob counter-

clockwise to remove the purge valve.

パージバルブ

(2) Using an 8-mm wrench, turn to remove

the needle guide counterclockwise.

(1)
(2)
Needle Guide

(3) Remove the packing from the guide, and

then install the new packing to the guide. Packing

Needle Guide

136
(4) Tighten the needle guide screw lightly
with your fingers.

(2)
(1)
Needle Guide

(5) Fix the purge valve to needle guide by

making approximately two turns.
Check if there are any spaces between
the purge valve and pump head.

Purge Valve

(6) Insert an 8-mm wrench to the spaces

between the pump head and the purge
valve, and tighten the needle guide.
And then turn to firmly fix the purge
(1)
valve.

(2)

(7) When the replacement has been completed, run the solvent leak test, and check if
solvents leak from the purge valve section.

137
8.13 Replacing Plunger Seal for the PU-4285-Binary
The plunger seal in the pump head stops solvent leaking as the plunger moves backward and
forward. When the plunger seal deteriorates, leaks begin and may lead to flow rate drop,
decrease of reproducibility due to unstable flow, or baseline variation.
JASCO recommends replacing the plunger seal at regular intervals, depending on the
frequency of instrument use and the kind of solvent being used. Also recommends replacing
the plunger seals in both pump heads at the same time.
Replace the solvent inside the pump with methanol or water (HPLC-grade) when replacing
the plunger seal.

CAUTION
Strictly obey the following instructions when replacing the plunger seal.

(1) Replace the solvent inside the pump with methanol or water (HPLC-grade) when
replacing the plunger seal.
(2) To replace the plunger seal, disassemble the pump parts. At this time, solvents
remaining in the pump may go out and they may stick in your fingers. Use chemical
groves to prevent putting solvents to you.
(3) Sometimes solvents may leak during the pressure ramp and holding test. In this case,
wipe out the solvents quickly using tissue. The tissue used for wiping should be
wasted after suitable treatments.
(4) Salts in buffer solutions may form crystals, causing poor instrument performance, so
do not use buffers for the pressure ramp and holding test.
(5) Note that the power is turned OFF, unless otherwise stated.

Tools required M4 hex key wrench (subtense: 3-mm)

8-mm wrench
Small flat-blade screwdriver
(precise flat-blade screwdriver)
Plunger seal pre-forming tool
Part Number
Parts required Plunger seal (2 pcs./pk) 7003-H302A

138
Removing the pump head

(1) Disconnect all the tubing from the pump

heads.

(2) Turn the purge valve knob counter-

clockwise to remove the purge valve.

(1)
(2)

Purge Valve

139
(3) Use an M4 hex key wrench (subtense:
3-mm) to loosen the four hex bolts on
(3)
the pump head evenly and alternately. When the back-up rings
(made of PEEK) remain in
the plungers, remove
Remove the pump heads. Slowly pull them.
out the pump heads straight in line with
the plunger. If they are pulled out at an
angle, the plunger may break. Delivery Head

The pump head has a delivery head (2)

and a modifier head. Remove each
head separately.
Modifier Head
When the backup rings (made of (1)
PEEK) remain in the plungers, remove
them.

Removing the plunger seal

(4) Using a small flat-blade screwdriver

(precise screwdriver), remove the
plunger seal and back-up ring (made by
PEEK).
Be careful not to scratch the inside
surface of the seal attachment hole
when you press the plunger seal with
the screwdriver.
If the inside surface of the seal
Plunger
attachment hole is scratched, it causes
Seal
solvent leaks. Back-up Ring

(5) If any plunger seal and back-up ring

Remove particles remain in
particles remain in the pump heads,
the pump head.
flush inside the pump head with
Methanol completely, and then wipe
using a chemical tissue.
Be careful not to scratch the inside
surface of the seal attachment hole.

140
(6) Dirt on the plunger ages the plunger seal. Remove using a chemical tissue which
mehanol is put. If particles cannot be removed easily, place some non-abrasive
household cleaner on a cloth and rub the plungers clean.

Installing the new plunger seal

(7) Press a new, unformed plunger Carefully press the plunger seal so
seal into the seal pre-forming that the edge is not damaged.
tool. Wait for a few minutes.
When pressing a new seal into
the seal pre-forming tool, care-
Press the plunger
fully press it straight in gently.
seal towards the
Note that the edge of the Plunger Seal
curving surface
plunger seal is easily Pre-forming Tool
side.
damaged.

Press on the push-up pole to

extract the seal, and install it
immediately.

(8) Install the new plunger seal to

the pump head. As the figure
shows, slowly press it straight
in with one finger. Plunger Seal
Be sure to press straight in
gently by your finger (not use
any tool), because the edge of
the plunger seal is easily
damaged.
Moistening the plunger seal
and the pump head seal hole
with methanol often makes
insertion easier.

141
(9) Next, put the backup ring in the
pump head. When installing
the ring, slowly press it straight
in with one finger.
Moistening the back-up ring
with methanol helps to attach
firmly to the seal.

Back-up Ring

Installing the pump heads

(10) Install the pump heads separately after

Check the back-up
checking the back-up ring is attached into
ring (made of PEEK)
the pump head.
is attached into the
When installing the pump heads, gently
pump head.
insert the pump head straight in line with
the plunger and guide pins. If it is not
inserted straight, the plunger may break
or the plunger seal may be damaged, Delivery

and it may cause solvent leak. Head

Modifier Head

142
(11) Use an M4 hex key wrench to tighten the
four hex bolts. Use a crisscross pattern to tighten the

Use a crisscross pattern to tighten the four hex bolts evenly.

four hex bolts evenly.

Be careful to tighten the bolts evenly
(crisscross pattern) and firmly.

(12) Install the purge valve.

Turn the purge valve

knob clockwise to install
the purge valve.

143
(13) Connect all the tubing.

Checking after replacing the plunger seal

(14) When the replacement has been completed, start the flow and check if solvents leak
from the plunger seal.

(15) Reset the information on the plunger seal replacement and the number of plunger
driving if necessary. See Section 6.5.1 for the setting.

8.14 Replacing Check Valve Assembly for the

PU-4285-Binary

Tools required 8-mm wrench

12-mm wrench
(Torque wrench (with 12-mm socket))
Part Number
Parts required Check valve assembly (IN) 7003-H143A
Check valve assembly (OUT) 7003-H144A

144
 CAUTION
Strictly obey the following instructions when replacing the check valve assembly.

(1) Replace the solvent inside the pump with methanol or water (HPLC-grade) when
replacing the check valve assembly.
(2) Note that the pump is stopped and is not pressurized. If pressurized, solvents may go
out and scatter.
(3) To replace the check valve assembly, disassemble the pump parts. At this time,
solvents remaining in the pump may go out and they may stick in your fingers. Use
chemical groves to prevent putting solvents to you.
(4) Sometimes solvents may leak during the pressure ramp and holding test. In this case,
wipe out the solvents quickly using tissue. The tissue used for wiping should be
wasted after suitable treatments.
(5) Note that the power is turned OFF, unless otherwise stated.

(1) Disconnect all the tubing and inlet tubing

from the pump heads.

145
(2) Use a 12-mm wrench or torque wrench
(with 12-mm socket) to remove the check
valve.

Check Valve
Assembly (OUT)

Check Valve
Assembly (IN)

Remember the following when removing and installing the check valve assembly.
1) Tighten the bolts securing the pump head before you loosen the check valve
assembly, or the plunger may break.
2) Loosen the pump-head bolts to their original tightness after the check valve
assembly has been replaced.
3) When turning the wrench, hold the pump steady.
4) The check valve assembly has an IN section and an OUT section. Note that the
direction.
5) When tightening the check valve assembly, be careful not to touch the wrench
with the plunger washing mechanism. If the wrench is turned with toughing to the
plunger washing mechanism, it may be damaged.

If replacing the check valve assembly is

difficult while the pump head is attached,
remove the pump head first (see Section
8.13).
While the pump head is being removed,
make sure it is held securely with an
adjustable wrench (see the figure in the
right) or in a vise.

146
(3) Check if there are any remaining gasket
materials or other contaminants from the
inside surface of the check valve
Check if there are any
assembly. If there are, clean them by
remaining gasket
rinsing the area with methanol in a
materials or other
syringe. And then wipe the inside surface
contaminants.
with a piece of clean cloth or chemical
tissue.

Installing the new check valve assembly

(4) Using a 12-mm wrench, install the check

valve assembly with moistening with
methanol.

If a torque wrench is available, set the

torque to 25 N.m, and then tighten the
check valve.
Check Valve
Assembly (OUT)

Check Valve
Assembly (IN)

147
 (5) Connect all the tubing.

Check after replacing the check valve assembly

(6) When the replacement has been completed, start the flow and check if solvents leak
from the check valve assembly.

8.15 Replacing Purge Valve Needle and Purge Valve

Packing for the PU-4285-Binary
When solvent leaks are found from the purge valve section, replace the purge valve needle or
purge valve packing.

Tools required M2 hex key wrench (subtense: 1.5-mm)

8-mm wrench
Part Number
Parts required Purge valve needle 7002-0403A
Purge valve packing (2 pcs./pk) 6696-H305A

148
 CAUTION
Strictly obey the following instructions when replacing the purge valve section.

(1) Salts in buffer solutions may form crystals, causing poor instrument performance, so
do not use buffers for the pressure ramp and holding test.
(2) Note that the pump is stopped. Solvent will leak if tubing is removed while the pump is
turned ON.
(3) Note that the power is turned OFF, unless otherwise stated.
(4) Before removing the purge valve needle, remove the inlet tubing or put the solvent
container below the level of the pump if necessary.

Replacing the purge valve needle

(1) Turn the purge valve knob counter-

clockwise to remove the purge valve.

Purge Valve

(2) Use an M2 hex key wrench (subtense:

1.5-mm) to remove the drain valve (2)
needle from the knob.
Needle

Knob (1)

(3) Use an M2 hex key wrench to install the new drain valve needle to the knob by the
reversed procedures in Step (2).

149
(4) Use a hex key wrench to fix the new needle to the knob.

(5) Install the purge valve to the pump.

(6) When the replacement has been completed, start the flow and check if solvents leak
from the purge valve section.

Replacing the purge valve packing

(1) Turn the purge valve knob counter-

clockwise to remove the purge valve.

Purge Valve

(2) Using an 8-mm wrench, turn to remove

the needle guide counterclockwise.

(1)
(2)
Needle Guide

(3) Remove the packing from the guide, and

then install the new packing to the guide. Packing

Needle Guide

150
(4) Tighten the needle guide screw lightly
with your fingers.

(2)
(1)

Needle Guide

(5) Fix the purge valve to needle guide by

making approximately two turns.
Check if there are any spaces between
the purge valve and pump head.

Purge Valve

(6) Insert an 8-mm wrench to the spaces

between the pump head and the purge
valve, and tighten the needle guide.
And then turn to firmly fix the purge
(1)
valve.

(2)

(7) When the replacement has been completed, run the solvent leak test, and check if
solvents leak from the purge valve section.

151
8.16 Replacing Magnetic Stirrer and Filter in the Mixing
Chamber for the PU-4185/4285-Binary
Stirrer bar is in the mixing chamber. It rotates at the speed of 1000 to 5100 rpm. Due to this
higher rotation, the surface of the stirrer bar will be worn when it is used for a long time. We
recommend checking the condition of the stirrer bar once a month. If the wearing is found,
replace with the new one.
Also, if solvents leaks from the filter retainer screw on the mixing chamber or if the operating
pressure is increased by clogging the filter, replace it with the new one.

Tools required 8-mm wrench

Phillips screwdriver
Part Number
Parts required Stirrer bar 2330-0010
Outlet filter assembly for the 6929-H104A
mixing chamber (2 pcs./pk)

CAUTION
Strictly obey the following instructions when replacing the stirrer bar and filter in the mixing
chamber.

(1) Note that the power is turned OFF, unless otherwise stated.
(2) Replace the solvent inside the mixer chamber with methanol or water (HPLC-grade)
when replacing the stirrer bar and filter.
(3) Disassembling the chamber is required to replace the stirrer bar and filter. At this time,
solvents remaining in the chamber may go out and they may stick in your fingers. Use
chemical groves to prevent putting solvents to you.
(4) Sometimes solvents may leak during the pressure ramp and holding test. In this case,
wipe out the solvents quickly using tissue. The tissue used for wiping should be
wasted after suitable treatments.
(5) Salts in buffer solutions may form crystals, causing poor instrument performance, so
do not use buffers for the pressure ramp and holding test.

152
Replacing the stirrer bar

(1) Remove the mixing chamber from the

pump.

(2) Loosen the filter retainer screw with

holding the mixing chamber.

(3) Remove the filter retainer screw.

153
(4) Take out the old stirrer bar inside the
chamber and then replace with the new
one.

(5) Tighten the filter retainer screw.

(6) Check if solvents leaks from the mixing chamber.

If it is OK, install the unit to the pump.

Replacing the filter

(1) Remove the mixing chamber from the

pump.

154
(2) Loosen the filter retainer screw with
holding the mixing chamber.

(3) Remove the filter retainer screw.

(4) Remove the filter assembly from the filter

retainer screw and then replace with the
new one.

155
(5) Tighten the filter retainer screw.

(6) Check if solvents leaks from the mixing

chamber.
If it is OK, install the unit to the pump.

156
8.17 Replacing the Power Fuses

WARNING!
(1) Always replace with power fuses of the same rating to prevent fire and other hazards.
(2) To avoid danger when replacing the power fuses, be sure to unplug the power cable
from the power terminal.

Note: Replace both power fuses even if only one power fuse has blown.

Table 8.1 shows the power fuses used in the pumps.

Table 8.1 Power Fuses for the Pumps

Voltage Power fuses
100 to 120 V T2.5A
200 to 240 V T1.6A

(1) Insert your finger on the fuse holder, pinch the holder, and pull. The power fuses and the
fuse holder will come out together (see Figure 8.2).

Note: If the fuse holder is difficult to remove, inset the tool which end is unguiform (e.g.
flat-blade screwdriver) from underneath the fuse holder, and carefully pull out the
holder a little. And then pull it out by your fingers.

(2) Remove the old power fuses from the fuse holder, insert new power fuses, and replace
the fuse holder.
(3) If newly replaced power fuses immediately blows out again, contact your local JASCO
distributor.

Power Fuses

Fuse Holder

Figure 8.2 Replacing the Power Fuses

157
9. Optional and Replacement Parts
9.1 Standard Accessories
This chapter describes the standard accessories for each pump.

Table 9.1 Standard Accessories for the PU-4086-Binary

Name Qty. Remarks
Tubing
1 Inlet tubing ASSY 2 Length: 2 m
2 PTFE tubing 2 2 mm OD x 1.4 mm ID x 1 m L
(for drain tubing)
Plunger washing mechanism
3 Tubing for plunger washing 4 Length: 1.5 m
mechanism
Electricity
4 Power cable 1 For tubing from/to pump and column
5 Slow-blow fuses 6 T2.5A (100 V system) x 2 pcs.
T1.6A (200 V system) x 4 pcs.
Plunger seal
6 Plunger seal pre-forming tool kit 1 Pre-forming tool and push-up pole for
PU-4086-Binary
Others
7 Drain Port (Bottom) 1 with two securing screws
8 End Drain Port 1
9 Tubing Holder 1 with two securing screws

Table 9.2 Standard Accessories for the PU-4185/4285-Binary

Name Qty. Remarks
Tubing
1 Inlet tubing ASSY 2 Length: 2 m
2 PTFE tubing 2 2 mm OD x 1.4 mm ID x 1 m L
(for drain tubing)
Plunger washing mechanism
3 Tubing for plunger washing 4 Length: 1.5 m
mechanism
Electricity
4 Power cable 1 For tubing from/to pump and column
5 Slow-blow fuses 6 T2.5A (100 V system) x 2 pcs.
T1.6A (200 V system) x 4 pcs.
Plunger seal
6 Plunger seal pre-forming tool kit 1 Pre-forming tool and push-up pole for
PU-4185/4285-Binary

158
 Others
7 Drain Port (Bottom) 1 with two securing screws
8 End Drain Port 1
9 Tubing Holder 1 with two securing screws

9.2 Start-up Kits and Maintenance Tool Kit

The Start-up Kits for HPLC, RHPLC/UHPLC, Preparative-LC (Prep-LC), and inert LC are
available. Use one of the kits depending on the measurement mode (HPLC, RHPLC, UHPLC,
Prep-LC, etc.). See each kit for details on the tubing.
In addition, Maintenance Tool Kit for LC-4000 series is available.

(1) LC-4000 HPLC Start-up Kit 7001-H401A

This is the kit for HPLC on a stainless steel tubing base.

Contents
Name Qty. Remarks
1 Pre-cut stainless steel tube (1) 1 For the tubing between pump
1/16-inch OD x 0.25 mm ID x 450 mm L outlet and autosampler inlet
2 Pre-cut stainless steel tube (2) 1 For the tubing between pump
1/16-inch OD x 0.25 mm ID x 350 mm L outlet and autosampler inlet
3 1/32-inch OD pre-cut stainless steel tube 1 For the tubing between
(1/16-inch OD ends) (1) autosampler outlet and
0.25 mm ID x 600 mm L column inlet
4 1/32-inch OD pre-cut stainless steel tube 1 For the tubing between
(1/16-inch OD ends) (2) autosampler outlet and
0.25 mm ID x 400 mm L column inlet
5 PEEK tube For the tubing between
1/16-inch OD x 0.25 mm ID x 5 m L column and Detector
6 Stainless steel tube 1 Spare tubing
1/16-inch OD x 0.25 mm ID x 5 m L
7 Fluoride tubing 2 Waste tubing from End Drain
1m Port
8 Drain tubing (L-shape SS) 1 Tubing for connecting the
1.0 mm ID PTFE mobile phase wasting
tubing form detector outlet
9 Back-pressure coil (SS) 1 Back-pressure coil connected
0.25 mm ID x 1 m to detector outlet
10 PTFE tubing 1 Mobile phase wasting tubing
1.4 mm ID x 2.0 mm OD x 2.5 m L
11 One-piece fingertight fitting 5 For connecting column outlet
and detector inlet/outlet
tubing

159
 12 Fitting, SS 10
13 Ferrule, SS 10
14 Rheodyne fitting (LONG) 3 For connecting the tubing on
the autosampler injection
15 Rheodyne ferrule 3 valve (spare)

16 Dead-volume-less union 1
17 Lure lock needle 1
18 Disposable syringe 10 mL (PP) 1
Lure chip type

(2) LC-4000 HPLC PEEK Start-up Kit 7001-H402A

This is the kit for inert-LC and HPLC on a PEEK tubing base.

Contents
Name Qty. Remarks
1 PEEK tube 1
1/16-inch OD x 0.25 mm ID x 3 m L
2 PEEK tube 1
1/16-inch OD x 0.5 mm ID x 3 m L
3 Fluoride tubing 2 Waste tubing from End Drain
1m Port
4 Back-pressure coil (PEEK) 1 Back-pressure coil connected
0.25 mm ID x 1 m to detector outlet
5 PTFE tubing 1 Mobile phase wasting tubing
1.4 mm ID x 2.0 mm OD x 2.5 m L
6 One-piece fingertight fitting 10
7 Rheodyne fitting (PEEK) 3 For connecting the tubing on
the autosampler injection
valve (spare)
8 Dead-volume-less union (PEEK) 1
9 Lure lock needle 1
10 Disposable syringe 10 mL (PP) 1
Lure chip type

160
(3) LC-4000 RHPLC/UHPLC Start-up Kit 7001-H403A
This is the kit for RHPLC/UHPLC.

Contents
Name Qty. Remarks
1 Pre-cut stainless steel tube (1) 1 For the tubing between pump
1/16-inch OD x 0.25 mm ID x 450 mm L outlet and autosampler inlet
2 Pre-cut stainless steel tube (2) 1 For the tubing between pump
1/16-inch OD x 0.25 mm ID x 350 mm L outlet and autosampler inlet
3 1/32-inch OD pre-cut stainless steel tube 1 For the tubing between
(1/16-inch OD ends) (1) autosampler outlet and
0.13 mm ID x 600 mm L column inlet
4 1/32-inch OD pre-cut stainless steel tube 1 For the tubing between
(1/16-inch OD ends) (2) autosampler outlet and
0.13 mm ID x 400 mm L column inlet
5 PEEK tube 1 For the tubing between
1/16-inch OD x 0.13 mm ID x 3 m L column outlet and detector
inlet (RHPLC), and for the
tubing between detectors
(RHPLC/UHPLC)
6 PEEK tube 1 For the tubing between
1/16-inch OD x 0.064 mm ID x 1.5 m L column outlet and detector
inlet (UHPLC)
7 High-pressure fitting, VHP-310 1 For connecting a column
(for column connection) (high-pressure)
Ground-breaking fitting
system, fingertight.
8 Fluoride tubing 2 Waste tubing from End Drain
1m Port
9 Drain tubing (L-shape SS) 1 Tubing for connecting the
1.0 mm ID PTFE mobile phase wasting
tubing form detector outlet

10 Back-pressure coil (SS) 1 Back-pressure coil connected

0.25 mm ID x 1 m to detector outlet
11 PTFE tubing 1 Mobile phase wasting tubing
1.4 mm ID x 2.0 mm OD x 2.5 m L
12 One-piece fingertight fitting 5 For connecting column outlet
and detector inlet/outlet
tubing

13 Fitting, SS 10
14 Ferrule, SS 10

161
 15 Rheodyne fitting (LONG) 3 For connecting the tubing on
the autosampler injection
16 Rheodyne ferrule 3 valve (spare)

17 Dead-volume-less union 1
18 Lure lock needle 1
19 Disposable syringe 10 mL (PP) 1
Lure chip type

(4) LC-4000 Preparative HPLC Start-up Kit 7001-H404A

This is the kit for Preparative HPLC.

Contents
Name Qty. Remarks
1 Stainless steel tube 1
1/16-inch OD x 0.5 mm ID x 5 m L
2 Stainless steel tube 1
1/16-inch OD x 0.8 mm ID x 5 m L
3 Fluoride tubing 2 Waste tubing from End Drain
1m Port
4 PTFE tubing 1 Mobile phase wasting tubing
1.4 mm ID x 2.0 mm OD x 2.5 m L
5 One-piece fingertight fitting 5
6 Fitting, SS 10
7 Ferrule, SS 10
8 Rheodyne fitting (LONG) 3 For connecting the tubing on
the autosampler injection
9 Rheodyne ferrule 3 valve (spare)

10 Dead-volume-less union 1
11 Lure lock needle 1
12 Disposable syringe 10 mL (PP) 1
Lure chip type

162
(5) LC-4000 Maintenance Tool Kit 7001-H405A

Contents
Name Qty. Remarks
1 Tubing cutter (for PEEK/PTFE tube) 1
2 Stainless steel tubing cutter 1

3 Hex key wrench (D=2.5, 3, and 4 mm) 1

each
4 Wrench (1/4-, and 5/16-inch, double head) 1
5 Wrench (5.5, 10, 12, and 14 mm, single 1
head) each
6 Precise Phillips screwdriver 1
7 Phillips screwdriver 1

163
10. Consumable Parts
10.1 Consumable Parts for the Inlet Tubing
Name: Replacement filter for the inlet tubing
Part Number: 2318-0065
Materials: Stainless steel
Pore size: 20 µm

164
10.2 Consumable Parts for the PU-4086-Binary
10.2.1 Consumable Parts of the Pump Head Section for the
PU-4086-Binary

(5)
(2) (6)

(2)

(5)

(1)

(6)
(3)

(1)
(4)

(3)

165
 Part Number Name
(1) 7002-0304B Delivery head body
(1)
(2) 6965-H105A Check valve ASSY (OUT)
(1)
(3) 6965-H104A Check valve ASSY (IN)
(4) 7004-H108A Spare plunger seal & Back-up ring (2 pcs./pk)
(5) 7002-H133A(2) Head mount flange ASSY
(6) 7002-H105A Rinssing C/V Assy set

(1) Check valve (IN) and check valve (OUT) for the PU-4086-Binary are compatible with
those for the X-LC 3180PC, PU-4180 and PU-4087.
(2) Head mount flange ASSY for the PU-4086-Binary is compatible with the PU-4180.

(1)

Part Number Name

(1) 7002-H103A Delivery head ASSY (with plunger seal &back-up
ring)

166
10.2.2 Consumable Parts of the Outlet Port Section for the
PU-4086-Binary

(5)

(6)

(7)

(1)

(2)
(3)

(4)

Part Number Name

(1) 6696-H305A(1) Drain gasket (2 pcs./pk)
(2) 6778-0306A(1) Drain valve needle guide
(3) 7002-0403A Drain valve needle
(4) 7002-0402A Drain valve knob
(5) 7004-0601A Drain valve manifold
(6) 7004-H301A Line filter kit (2 set/pk)
(1)
(7) 6688-0602C Filter retainer screw

(1) Drain gasket, DV guide, and filter retainer screw are common for all the LC-4000 series
pumps.

167
10.3 Consumable Parts for the PU-4185Binary
10.3.1 Consumable Parts of the Pump Head Section for the
PU-4185-Binary

(6)

(11) (12)
(2)

(11)
(1)

(7)
(8)

(12)
(5) (4)

(9)

(10) (3)

168
 Part Number Name
(1) 7003-3101F Delivery head body
(2) 6949-H105A Check valve ASSY (OUT)
(3) 6949-H104A Check valve ASSY (IN)
(4) 7003-H301A Spare plunger seal & Back-up ring (2 pcs./pk)
(5) 7003-3201F Modifier head body
(6) 7003-0401YA Head tubing
(1)
(7) 6696-H305A Drain gasket (2 pcs./pk)
(8) 6778-0306A(1) Drain valve needle guide
(9) 7002-0403A(2) Drain valve needle
(10) 7002-0402A(2) Drain valve knob
(3)
(11) 7003-H133A Head mount flange ASSY
(12) 7002-H105A Plunger washing mechanism ASSY

(1) Drain gasket and D/V guide are common for all the LC-4000 series pumps.
(2) Drain needle and drain knob are common for all the LC-4000 series pumps (except inert
pumps).
(3) Head mount flange ASSY for the PU-4185-Binary is compatible with the
PU-4285/4285-Binary.

(1) (2)

Part Number Name

(1) 7003-H131A Delivery head ASSY (with plunger seal &back-up
ring)
(2) 7003-H132A Modifier head ASSY (with plunger seal &back-up
ring)

169
10.4 Consumable Parts for the PU-4285-Binary
10.4.1 Consumable Parts of the Pump Head Section for the
PU-4285-Binary

(6)

(11)

(12)
(2)

(1) (11)

(7) (4)
(8)
(5)
(12)
(3)

(9)

(10)

170
 Part Number Name
(1) 7003-3401F Delivery head body
(2) 7003-H144A Check valve ASSY (OUT)
(3) 7003-H143A Check valve ASSY (IN)
(4) 7003-H302A Spare plunger seal & Back-up ring (2 pcs./pk)
(5) 7003-4201F Modifier head body
(6) 7003-0401YA Head tubing
(7) 6696-H305A(1) Drain gasket (2 pcs./pk)
(8) 6778-0306A(1) Drain valve needle guide
(9) 7002-0403A(2) Drain valve needle
(10) 7002-0402A(2) Drain valve knob
(11) 7003-H133A(3) Head mount flange ASSY
(12) 7002-H105A Plunger washing mechanism ASSY

(1) Drain gasket and D/V guide are common for all the LC-4000 series pumps.
(2) Drain needle and drain knob are common for all the LC-4000 series pumps (except inert
pumps).
(3) Head mount flange ASSY for the PU-4285-Binary is compatible with the
PU-4185/4185-Binary.

(1) (2)

Part Number Name

(1) 7003-H141A Delivery head ASSY (with plunger seal
&back-up ring))
(2) 7003-H142A Modifier head ASSY (with plunger seal
&back-up ring)

171
10.5 Optional Cables for the Pumps
The optional cables that can connect to all the LC-4000 series pumps are listed in Table 10.1.

Table 10.1 Optional Cables for All the LC-4000 Series Pumps
Name Qty. Part Number Remarks
Analog signal output cable 1 5590-6313A Length: 2100 mm

Plug-in phone jack (6-pin) Terminal bar (3-pin)

(Use)
This is used when operating pressure signal is output to the LC-Net/II ADC. Connect
three terminal bars to the “PRESS SIGNAL” terminals on the rear panel and connect the
plug-in phone jack to the LC-Net/II ADC to output the operating pressure.

Marker cable 1 5590-6631A Length: 1300 mm

Terminal bar (2-pin) Terminal bar (2-pin)

(Use)
Both ends of the cable are 2-pin terminal bars. Connect the cable to “RESET/RUN”,
“PUMP ON”, “PUMP OFF”, and “STOP OUT” terminals of the input/output terminals on
the rear panel.
See Section 3.4.3 for the connection method. Also, example use of “PUMP OFF” and
“STOP OUT” terminals are shown in Section 3.4.4.

172
11. Options for the Mixing Unit
The mixing chamber in the mixing unit is changeable.
The procedures for removing and installing the chamber are shown as follows.

(1) Remove the mixing chamber from the

pump.

(2) Prepare the optional mixing chamber.

(3) Install the mixing chamber.

173
Table 11.1 lists the changeable mixing chambers and Table 11.2 lists the consumable parts
for each optional mixing chamber.

Table 11.1 Analytical Mixing Chambers

Name Part Number Maximum Pressure Wetted Materials
50 µL mixing chamber 7007-H141A
100 µL mixing chamber 7007-H142A
250 µL mixing chamber 7007-H143A
130 MPa Stainless steel
400 µL mixing chamber 7007-H144A
(SUS316), PEEK,
800 µL mixing chamber 7007-H145A
PTFE
1.5 mL mixing chamber 7007-H146A
3.0 mL mixing chamber 7007-H147A
50 MPa
5.0 mL mixing chamber 7007-H148A

Table 11.2 Consumable Parts for the Mixing Chambers

Name Chamber Volume Qty. Part Number
50 µL
1 pc. 2330-0015
100 µL
250 µL
400 µL
Stirrer bar 800 µL 1 pc. 2330-0010
1.5 mL
3.0 mL
5.0 mL
3 pcs./pk 6783-H111A
10.0 mL
50 µL
100 µL
2 pcs./pk 6929-H104A
250 µL
400 µL
Line Filter Kit 800 µL
2 pcs./pk 7007-H341A
1.5 mL
3.0 mL
5.0 mL 2 pcs./pk 7007-H342A
10.0 mL

174
 JASCO Corporation
2967-5, Ishikawa-machi, Hachioji-shi
TOKYO, JAPAN

Printed in Japan