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WBC Counting Techniques in Neubauer Chamber

1. The document provides instructions for several laboratory procedures related to analyzing blood samples under a microscope. It details the proper techniques for focusing different parts of the blood in various microscope configurations and preparing blood smears and samples. 2. The procedures include focusing white blood cells and red blood cells in counting squares, making a peripheral blood smear, focusing a neutrophil under oil immersion, mounting a blood sample for an ESR test, charging a Neubauer chamber, converting blood to acid haematin, and filling a Wintrobe tube. 3. Correct microscopic adjustments, handling of instruments and reagents, and avoidance of issues like bubbles or over/undercharging are emphasized across the different lab techniques.

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Ajay Sriram
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47 views3 pages

WBC Counting Techniques in Neubauer Chamber

1. The document provides instructions for several laboratory procedures related to analyzing blood samples under a microscope. It details the proper techniques for focusing different parts of the blood in various microscope configurations and preparing blood smears and samples. 2. The procedures include focusing white blood cells and red blood cells in counting squares, making a peripheral blood smear, focusing a neutrophil under oil immersion, mounting a blood sample for an ESR test, charging a Neubauer chamber, converting blood to acid haematin, and filling a Wintrobe tube. 3. Correct microscopic adjustments, handling of instruments and reagents, and avoidance of issues like bubbles or over/undercharging are emphasized across the different lab techniques.

Uploaded by

Ajay Sriram
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

OSPE

1. Focus WBC counting squares in Neubauer’s chamber under low


power:
Do the Bring the Just open Focus WBC Total
microscopic condenser the iris counting ( 5 marks)
adjustments to the diaphragm squares
for low lowest (1 mark) (1mark)
power position
a. Concave (1mark)
mirror
b. 10 X
objective
(2
marks)

2. Prepare an Ideal Peripheral Smear:


Select Place Place the Draw the Move the Total
slide and moderate spreader spreader spreader 5 marks
spreader sized drop in front of slide from right
(1mark) on the blood backwards to left,
right end drop at an to touch slowly and
1 cm away angle of the drop smoothly
from the 45’ so that (1mark)
edge of (1mark) blood
the slide spreads
(1mark) across the
edge of the
spreader
(1 mark)

3. Focus a neutrophil from the given peripheral smear under oil


immersion:
Do the Bring the Open the Focus a Total
microscopic condenser iris neutrophil (5 marks)
adjustments to the diaphragm (1 marks)
for oil highest fully
immersion position. (1 mark)
a. Plane (1 mark)
mirror
b.100 x
objective
(2 marks)
4. Focus RBC counting squares in Neubauer’s chamber under high
power:

Do the Bring the Half open Focus RBC Total


microscopic condenser the iris counting (5marks)
adjustments for in mid diaphragm squares
high power position (1 mark) (1 mark)
a. Concave (1 mark)
mirror
b. 45x
objective
(2 marks)

5. Mount the blood for ESR in the ESR tube:


Fix the Fill the Fix the Note the Total
pipette in pipette by upper time of (5marks)
the test tube rotating miniscus of mounting
containing clockwise the blood to (1 mark)
blood (1 mark) zero mark
(1 mark) (2 marks)

6. Charge the Neubauer’s chamber:


Clean the Place the Take the Avoid Charge the Total
Neubauer’s cover slip diluted overcharging other side (5marks)
chamber and on the blood kept or under (1 mark)
cover slip central in the charging
(1 mark) platform of pipette and (1 mark)
the charge the
chamber chamber by
covering holding the
the ruled pipette at
squares an angle of
(1 mark) 45°
between
the cover
slip and
chamber
(1 mark)
7. Convert a known volume of blood into acid haematin using
Sahli’s haemoglobinometer:
Fill the Take blood Gently wipe Insert Total
hemoglobin upto 20 cu the exterior pipette into (5marks)
tube with mm mark in of the tip of hemoglobin
N/10 Hcl up the the pipette tube
to lowest hemoglobin (1 mark) containing
mark pipette N/10 Hl, &
(2 g ms%) (1 mark) blow out the
(1 mark) blood. Rinse
the pipette
2-3 times
with the
acid present
in the tube
wait for 10
minutes for
the
formation
of acid
haematin.
(2 marks)

8. Fill blood in the Wintrobe’s tube with blood for determining


haematocrit:
Select the Take anti Fill the Total
Wintrobe’s coagulated Wintrobe’s (5marks)
tube blood in the tube from
(1 mark) 5 ml syringe below
provided, upwards
using exactly up
lumbar to the mark
puncture 10 without
needle air bubbles.
(2marks) (2marks)

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