7

Science
Quarter 2 – Module 2
Life Through the Lens…

i
Science – Grade 7
Self-Learning Module
Quarter 2 – Module: Life Through the Lens
First Edition, 2020

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Published by the Department of Education

Secretary: Leonor Magtolis Briones
Undersecretary: Diosdado M. San Antonio

Development Team of the Module

Writer: Jilea A. Yson

Editors: Cynthia S. Bustillo, Miraflor O. Albios, Lenie G. Forro, Leoncio Tan
Reviewers: Agabai S. Kabdalayang, Yusof A. Aliudin, Mary Joy D. Bautista,
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Ar O. Espartero
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Introductory Message

This Self-Learning Module (SLM) is prepared so that you, our dear learners,
can continue your studies and learn while at home. Activities, questions,
directions, exercises, and discussions are carefully stated for you to
understand each lesson.

Each SLM is composed of different parts. Each part shall guide you step-by-
step as you discover and understand the lesson prepared for you.

Pre-test are provided to measure your prior knowledge on lessons in each

SLM. This will tell you if you need to proceed on completing this module, or
if you need to ask your facilitator or your teacher’s assistance for better
understanding of the lesson. At the end of each module, you need to answer
the post-test to self-check your learning. Answer keys are provided for each
activity and test. We trust that you will be honest in using these.

In addition to the material in the main text, Notes to the Teachers are also
provided to the facilitators and parents for strategies and reminders on how
they can best help you on your home-based learning.

Please use this module with care. Do not put unnecessary marks on any
part of this SLM. Use a separate sheet of paper in answering the exercises
and tests. Read the instructions carefully before performing each task.

If you have any questions in using this SLM or any difficulty in answering
the tasks in this module, do not hesitate to consult your teacher or
facilitator.

Thank you.

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 What I Need to Know

Hello! How are you? Have you ever wondered about the things that we
cannot see? Take a look at your hands, it may look clean but if we look it
under a device that could magnify tremendously it actually contains lots and
lots of microorganisms.
This module will open your eyes to the things that you could not ever
imagine. Life is pretty interesting, and at the microscopic scale, it can also be
beautiful, strange, intriguing, frightening and gross. Be a scientist yourself,
let’s work hand in hand as we find out the process on focusing and proper
handling of microscope.
Most Essential Learning Competency:
Focus specimens using the compound microscope.
This module is divided into two lessons:
Lesson 1 – Microscopy
Lesson 2 - The Magnification Process
After going through this module, you are expected to:
1. focus specimens using the compound microscope;
2. compute the magnification of the specimen; and
[Link] the proper ways of handling a microscope.

What I Know
Directions: Choose the best answer from the given choices Write
the letter of your choice on a separate sheet of paper.
1. To focus on a specimen, it is best to start with which objective lens?
A. Middle Magnification
B. Lowest Magnification
C. Highest Magnification
D. Intermediate Magnification
2. Which one of the following instruments would you use to see a plant
cell?
A. Telescope
B. Perisco
pe
C. Micros
cope
D. Kaleido
scope
3. On a typical microscope, there is a rotating part containing three
different objective lenses. What is this part called?
A. Stage

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 B. Eyepiece
C. Nosepiece
D. Focus Knob

4. This is turned to bring the object being viewed closer is __________.

A. cover slip
B. focus
knob
C. eyepiece
lens
D. objective lens
5. The lens that is within the eyepiece of the light microscope is called the
____.
A. ocular
B.
scanning
C. low
power
D. high power

6. When using the high-power objective, you should not adjust the
________.
A. fine focus
B. diaphragm
C. stage clips
D. coarse focus
7. The scanning, low, and high-power objectives are mounted on
the______.
A. stage
B. eyepiece
C. body tube
D. revolving nosepiece
8. Which part of the compound microscope helps in gathering and
focusing light rays on the specimen to be viewed?
A. Eyepiece lens
B. Objective
lens
C. Condenser
lens
D. Magnifying lens
9. On the microscope stage, what is used to hold the glass slide in place
and prevent it from moving?
A. Stage
B. Stage clip
C. Condenser
D. Fine adjustment knob
10. How does magnification affect the image of the objects?
A. It increases the image size

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 B. It decreases the image size
C. It increases the field of view
D. It decreases the field of view
11. After using the microscope, we need to clean it so that it will not get
rust easily. Which is NOT an essential step in cleaning the microscope?
A. Blow away dust
B. Wash the base and arm
C. Clean off grease and spills
D. Oil the mechanics and moving parts
12. Why is it important to use a staining element in viewing especially
colorless specimen?
A. To protect the specimen.
B. To keep the specimen flat.
C. To see clearly the specimen.
D. To bring out the starch in the specimen.
13. When viewing a sample of cheek cells using a microscope, the student
placed the cells on a slide and placed a cover slip over it. What is the
purpose of the cover slip?
A. To keep the cells flat.
B. To protect the cheek cells.
C. To get a better view of the cells.
D. To let the cheek cells, stay on the slide and not fall of it.
14. Dan prepared a thin sheet of onion skin and study using the
microscope but he cannot view clearly the specimen. Should Dan
adjust the coarse adjustment knob first then followed by the fine
adjustment knob?
A. No, because the draw tube and the body tube should be adjusted for
clearer view.
B. No, because fine adjustment knob must be adjusted first then the
coarse adjustment knob.
C. Yes, because the coarse adjustment knob is for the tilting of the
microscope and fine adjustment knob is for the rotation of the
revolving nosepieces and its lenses.
D. Yes, because coarse adjustment knob is for the fast movement of the
body tube and fine adjustment knob is for the slow movement of the
body tube
15. Lily examine a specimen but there was no image being viewed because
the field of view is dark. Should Lily adjust the objective lenses?
A. Yes, Lily should adjust the objective lenses because it is
responsible in providing light.
B. Yes, Lily should adjust the objective lenses because she can choose
between low power and high power.
C. No, Lily should adjust the diaphragm and the mirror because these
parts are responsible in providing of light.
D. No, Lily should adjust the fine adjustment knob and coarse
adjustment knob because these parts are for giving light.

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Lesson
Microscopy
1

What’s In
In the previous lesson, you already learned how to
identify the parts of the microscope and their function. Each part of the
microscope has its own function that is really important and essential to the
microscope as a whole.
Scrambled Letters
Directions: Listed below are parts of the microscope that are scrambled. Mix
the letters to come up with the right answer. Write your answer on a separate
sheet of
paper. [Link] = ____________________________
[Link] = ____________________________
[Link] = ____________________________
[Link] BTUE = ____________________________
[Link] = ____________________________
[Link] = ____________________________
[Link] = ____________________________

What Am I?
Directions: Identify the words that are being describe. Choose your answer
from
the box below. Write your answer on a separate sheet of paper.
BASE REVOLVING NOSEPIECE

STAGE ARM DIAPHRAGM

1. Supports the upper part of the microscope.

2. Located under the stage, controls the amount of light allowed to pass
through the object.
3. Small platform where the specimen is placed for evaluation.
4. Holds objective lenses and can be rotated.
5. The bottom part of the microscope, supports the entire microscope.

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 What’s New
Hello there! You are already familiar with the parts and functions of a
microscope. Let’s discover together how to focus specimen using the
compound microscope. All we have to do is read and understand the context
below to find these steps, are you ready? Let’s start!

Activity 1
Newspaper Letter “e”
Materials needed:
Newspaper Glass slides Dropper
Scissors Cover slides Microscope
Pencil Water
Procedures:
A. Cut out the letter “e” and place it on the slide face up.
B. Add a drop of water to the slide.
C. Place the cover slip over the “e” and water at a 45-degree angle.
D. Lower slowly with a pencil to keep air bubbles out.

1. Place the slide on the stage and view in low power. Center the “e” in your
field of view. Draw what you see? Move the slide to the left, what happens?
Move the slide to the right, what happens? Up? Down?
2. View the specimen in high power. Use the fine adjustment only to focus (this
keeps from breaking or scratching glass slides and objectives).
3. These are the images before and after examining under a microscope.
Naked Eye Low Power High Power

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 e

This is the actual letter This is an illustration of This is an illustration of

‘e’ before putting it a letter ‘e’ shown using the letter ‘e’ shown using
under the microscope. low power objective. high power objective.
This slide was set up Notice, that as you
with the letter in the increase the power of the
correct orientation. lens, your field of view gets
Notice that it appears smaller. Same with its
orientation, if you move the
upside down when slide to the left, the
viewed under the image goes right and vice
microscope. versa.

Guide Questions:
1. Did the letter appear in the same orientation when viewed through the
microscope as viewed without the microscope?
2. When you move the slide to the right what direction does it appear to move
under the microscope?
3. What happened to the image when you switched objectives?

Activity 2
Onion Cells Under the Microscope
Materials needed:
• Onion Glass slides Dropper
• Knife Cover slides Microscope
• Forceps Iodine solution Paper towels
Procedures:
1. Set aside a clean microscope slide.
2. Carefully cut away a small, single layered piece of onion (1-2 cm wide).
3. Peel the thin layer of skin (membrane) from the inside surface of your piece of
onion. Forceps may help with this. The membrane looks a bit like soft Scotch
tape and should separate relatively easily from the inside surface of the onion
slice.
4. Place section of membrane carefully on the microscope slide, trying to keep it
as flat as possible.
5. Apply a couple of drops of iodine solution to the section. Wait 2 minutes for
the stain to develop before positioning a cover slip over the section.

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6. Place the slide first under scanning objective, then low power objective and
finally under high power objective and observe the onion cells. *note: Be
careful, when focusing the microscope, not to break the glass slide with the
microscope lens.
Low Power High Power
This is an This is an
illustration of an illustration
onion skin of an onion
shown using skin shown
low power using high
objective. power
Notice that it is objective.
composed of The same
rectangle like with letter
shapes with “e” activity
dots on some of that as you
it. These rectangle increase the power of the lens, your
shapes are plant cells and the small dots field of view gets smaller. The
are nucleus. Plants cells are usually nucleus becomes more visible. By
rectangular and animal cells are circular. adding the stain or iodine solution
Plant and Animal Cells will be discussed the image is clearer and more visible
further on the next module. than without.

These are the parts of onion (plant) cells that are visible under the microscope.
• Cytoplasm- jelly like substance inside a cell where organelles are embedded.
• Cell wall- a rigid wall that surround and supports the cell.
• Nucleus- the central part of most cells that contains genetic material.

Directions: Draw and label the images of onion skin that you see on the
microscope under low power objective and high -power objective. Compare
what is the difference with both images. Write your answer on a separate
sheet of paper.
Low Power High Power

Comparison:

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 What is It
How to Focus a Microscope
A microscope can help you observe things that you can't see with the
naked eye, such as bacteria. However, if you do not know how to focus your
microscope correctly, you will be unable to view these objects. Getting the
best focus on your microscope requires setting it up properly, focusing on
your sample, and magnifying the sample so that you can see it.

Procedures:
1. Uncover the microscope, unwrap the cord, and plug it in or face the
mirror in an area with enough light source.
2. Clean all glass surfaces with lens paper. Start with the eyepiece, then do
the objectives, the substage mirror or illuminator, and end with the stage
window.
3. Turn on the light (or adjust the mirror so that the light shines through
the stage window). Adjust the diaphragm under the stage so that a
medium amount of light gets through. Adjust the fine focus knob to a
middle position.
4. Place the slide on the stage, with a slide clip holding down each side of
the slide. Center the specimen on the slide over the stage window.
5. Using the low power objective, focus with coarse adjustment. Start with
the objective as close to the slide as possible and focus away from the
slide.
6. Before turning to a higher power, make sure there is something
worthwhile to look at and center the specimen in the middle of the field
7. Without changing the focus adjustment, change to the next highest
objective (the centered specimen should be in view and should be almost
in focus). Now focus with the fine adjustment knob. Repeat Steps 6 and 7
up to the highest power.
8. Clean up! Return to low power objective before taking off the slide. Clean
and dry the stage and objectives, turn off the light, and wrap the cord
neatly around the base of the microscope. Replace the cover.

Guide Questions:
1. When the specimen is out of focus, which part of the microscope
should you turn to obtain a clearer image?
2. After switching from low power to high power the area of field will
appear? 3. Why is it important to start viewing from low power to high
power objectives?

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 How to Use A Microscope to Observe A Specimen?
1

Put a table lamp in front of the

microscope.

2
Put a low power eyepiece into the
microscope.

Rotate a low-power objective into

position.

Look through the eyepiece. Adjust the

mirror until you see a bright circle of
light.

5
Place a slide on the stage, and fix it with
the clips. The specimen on the slide
should be right above the hole of the
stage.

6
Look at the microscope from the side.
Turn the coarse adjustment knob
carefully to lower the objective until it is
very close to the slide.

7
Look through the eyepiece. Turn the
coarse adjustment knob to raise the
objective slowly until you can see an
image of the specimen

8
Turn the fine adjustment knob to get a
sharper image. The image is now in
focus.

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 What’s More

Wet Mounting a Specimen

In a wet mount, the specimen is places in a drop of water or other
liquid held between the slide and the cover slip by surface tension. This
method is commonly used, for example, to view microscopic organisms that
grow in pond water or other liquid media, especially when studying their
movement and behavior.
Materials: Slide, Cover slip, Water, Dropper, Tweezers and Specimen.
Procedures:
1. Place a drop of water on the center of a clean dry slide
2. Using the tweezers, place the specimen in the middle of the drop.
3. While holding the cover slip upright, carefully place one edge of the cover
slip next to the water.
4. Slowly lower the upper edge of the cover slip onto the water. The objective
is to minimize or eliminate air bubbles under the cover slip. You might
find it helpful to use one toothpick to hold the lower edge in place, while
using another to carefully lower the slip into place.
5. An absorbent towel can be placed at the edge of the cover slip to draw out
some of the water, further flattening the wet mount slide.

Guide Question:
Why would you want to wet mount a specimen?

What I Have Learned

Directions:Read the paragraph carefully and identify the correct words that fit in
the given sentences in the box below. Write your answer on a separate sheet of
paper.

Diaphragm Objectives Low Power

High Power Lens Paper Stage

Uncover the microscope, unwrap the cord, and plug it in or face the mirror
in an area with enough light source. Clean all glass surfaces with (1)
_______________.
Start with the eyepiece, then do the (2) _________________, the substage mirror
or illuminator, and end with the stage window. Turn on the light (or adjust the
mirror so that the light shines through the stage window).

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 Adjust the (3) ___________ under the stage so that a medium amount of light gets
through. Adjust the fine focus knob to a middle position. Place the slide on the
(4) ____________, with a slide clip holding down each side of the slide. Center the
specimen on the slide over the stage window.
Using the low power objective first, focus with coarse adjustment. Start with the
objective as close to the slide as possible and focus away from the slide. Before
turning to a (5) _____________ objective, make sure there is something worthwhile
to look at and center the specimen in the middle of the field.
Without changing the focus adjustment, change to the next highest objective
(the centered specimen should be in view and should be almost in focus). Now
focus with the fine adjustment knob.

Clean up! Return to (6) _________________ objective before taking off the slide.

Clean and around thedry the stage a base of the microscope. Replace the cover

and objectives, turn off the light, and wrap the cord neatly.

What I Can Do

Microscope in one of the most important inventions in the history, without

this instrument we would not be able to identify the greatest threats in
mankind even though they are just around us, because we cannot see them.
With the help of this instrument, in no time we could create a vaccine or cure
to this deadly virus and continue to live our lives alongside t o our love
ones.
Directions: In dedication for our front liners especially to hospital employees,
write a thank you message for all of their hard works and sacrifices being
made in this global health crisis, COVID 19 Pandemic. Write your message on
a separate sheet of paper.

Assessment

Directions: Choose the best answer from the given choices. Write the letter of
your choice on a separate sheet of paper.
1. Which focusing knob can be used with low power?
A. Diaphragm
B. Inclination Knob
C. High power objective
D. Fine adjustment knob
2. How many objective lenses are there in a compound microscope?

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 A.1
B. 3
C. 6
D. 12
3. Where should a specimen be positioned under a microscope under low
power to view it correctly?
A. Middle
B. Bottom
C. Left side
D. Right side
4. To focus a specimen, it is best to start with which objective:
A. Ocular
B. Scanning
C. Low power
D. High power
5. Which part of the microscope is used to sharpen the focus of the
specimen?
A. Fine adjustment knob
B. Coarse adjustment knob
C. Low power objective lens
D. High power objective lens
6. Which part of the compound microscope helps in gathering and focusing
light rays on the specimen to be viewed?
[Link] lens
[Link]
lens
[Link]
lens D.
Magnifying
lens
7. The object to be studied and placed on the stage of the microscope is
described as the ____________.
A. clip
B. slide
C. image
D. specimen
8. Another term for the eyepiece is __________.
A. Mirror
B. ocular
C. cover slip
D. objective lens
9. The area that can be seen through the eyepiece of a microscope is the
_____.
A. Image
B. Specimen
C. Field of view
D. Magnification

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 10. When preparing a wet mount for a study under the microscope, what will
you do to ensure that the liquid portion of the specimen will not spill on it.
A. Cover slip is placed over the specimen.
B. Low power lens is above the object on the stage
C. Drop of methylene blue is added to the specimen
D. Drop of water is added to the specimen on the slide

What I Know

Directions: Choose the best answer from the given choices. Write the letter of
your choice on a separate sheet of paper.
1. A student wants to view cells under the compound microscope at a total
magnification of 400X. If the eyepiece is 10X, which of the following
objective lenses should be used?
A. 5X
B. 10X
C. 40X
D. 80X
2. If the eyepiece of a microscope magnifies by 10, and the objective lens
magnifies by 4, what is the total magnification of the microscope?
A.0.4
B. 2.5
C. 14
D. 40
3. If the eyepiece magnification on light microscope is x10 and the objective is
x40, what is the overall magnification?
A. X4
B. X10
C. X40
D. X400
4. What is the total magnification of a microscope of a microscope with two
lenses when one lens has a magnification of 15x and the other lens has a
magnification of 30x?
A. 15x
B. 30x
C. 45x
D. 450x
5. When observing cells, which of the following optical tools is most
appropriate?
A. Telescope
B. Petri dish
C. Hand lens

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 D. Microscope
6. Which part of the microscope is responsible for gathering diffuse rays from
the microscope light source and illuminating the specimen with a small
cone of bright light?
A. Ocular lens
B. Objective lens
C. Focusing knob
D. Condenser lens
7. What part will you adjust if you need to tilt the microscope?
A. Arm
B. Base
C. Diaphragm
D. Inclination Joint
8. Which two parts of the light microscope magnify the image of an object?
A. Eyepiece and mirror
B. Objective and mirror
C. Objectives and diagram
D. Eyepiece and objectives
9. Moves the stage slightly to sharpen the image is called ___________.
A. aperture
B. body tube
C. fine focus knob
D. coarse focus knob
10. Which should be used to observed bacteria?
A. 43x objective and 10x eyepiece
B. 60x objective and 15x eyepiece
C. 97x oil immersion objective and 5x eyepiece
D. 60 x oil immersion objective and 10x eyepiece
11. Which of the following statement is true?
A. All living things are made of cells.
B. Bacteria are cells that have bad effects.
C. New cells are created when old cells die.
D. A hen’s egg is an example of many millions of cells.

12. What do you think is the reason why a scratch on microscope lenses
happened?
A. Washing the entire microscope.
B. Providing too much light when viewing.
C. Using rough cloth in cleaning the lenses.
D. Keeping the microscope in a cabinet full of dust.
13. How are you going to handle a microscope when transferring from one
location to another?
A. Hold the microscope on the stage with both hands.
B. Hold on the arm and swing it as you walk to keep in balance.
C. Hold it in the inclination joint with one hand and other hand to its
base.

17
 D. Hold one hand on the arm of the microscope and other hand on the
base of the microscope.
14. The glass slide has a rectangular shape, it is where the specimen should be
placed. A student knows that after using the materials in their experiment
he should clean them before keeping it. He decided to put the specimen at
the edge of the glass slide so that cleaning of it afterwards can be easily
done. Did the student do the right thing?
A. No, because he should place it at the tip of the glass slide.
B. Yes, because it will be easy in cleaning the materials afterwards.
C. No, because a specimen should be placed on the center of the slide.
D. Yes, because it will still look the same whether it is placed on the
center or the edge.
15. A student is task to solve the magnification of the microscope lenses that he
has used. Should he multiply the value of ocular lens and the value of
objective lenses?
A. No, because he should add the value of ocular lens and the
value of objective lenses.
B. No, because he should divide the value of ocular lens and the value
of objective lenses.
C. Yes, because the sum of the ocular lens and objective lenses are
equals to the total magnification.
D. Yes, because the product of the ocular lens and objective lenses are
equals to the total magnification.

Lesson

2 The Magnification Process

What’s In

In the previous lesson, you already learned how to focus specimen using a
compound microscope. You learned that each part of the microscope has its own
role in focusing the specimen.
Mix and Match
Directions: Listed below are parts of the microscope that are scrambled. Mix the
letters in the column B to come up with the right answer and match it to column
A which is their function. Write the letter only on a separate sheet of paper.

18
 Column A Column B

1. Support the upper part of the 1. RRIORM

microscope and used to carry it.

[Link] the eyepiece in place 2. MRA

[Link] light for viewing the 3. ETSAG

image
[Link] the objective lenses 4. WRDA BTUEH

5. Platform where specimen is 5. EOSNEEIPC

placed.

Directions: Write TRUE if the statement is correct and FALSE if the statement is
wrong. Write your answer on a separate sheet of paper.
1. When you move the slide to right the image moves to the left.
2. When you move the slide away from you, the image will also move away from
you.
3. Use the low power objective before using the high-power objective.
4. Clean all glass surfaces with tissue paper.
5. Adjust the diaphragm under the stage so that a medium amount of light gets
through.

What’s New

Hello there! You are already learned how to focus specimen using a
microscope. Now let’s discover together how to compute the magnification of the
specimen and know the proper ways of handling the microscope. All we have to do
is read and understand the context below to find these, are you ready? Let’s start!

Activity 1
Magnification is the ability to view an object as larger. A good image is
obtained when the amount of specimen detail is also increased. To determine the
total magnification, multiply the magnification of eyepiece by the objective lens.
Total Magnification
Directions: Fill in the charts below. Calculate the total magnifications for the
examples given, then calculate the total magnification using each of the objective
lenses on microscope scenario. Write your answer on a separate sheet of paper.
*Eyepiece lens magnification x objective lens magnification = Total Magnification
SAMPLES
Eyepiece Lens Objective Lens Total Magnification

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 Magnification Magnification
5x 50x 1.
5x 100x 2.
10x 50x 3.
20x 20x 4.

Activity 2: Proper Care and Maintenance

Directions: Tick (√) DO’s if the statement signifies proper care and maintenance
and Cross (X) if it isn’t. Write your answer on a separate sheet of paper.
[Link] carrying your microscope, hold [Link] ensure the objectives are
it by the base and the stage. cleaned immediately after use.
[Link] an annual basis moving parts on [Link] use sharp instruments or
the microscope should be cleaned and anything abrasive on the microscope
lubricated. lenses.
[Link] sure you store your microscope [Link] keep your microscope covered
in an area that has corrosive chemical when not in use even if the microscope is
fumes stored in a cabinet.
[Link] the lenses of the microscope to [Link] should never allow the lens to
ensure their safety of lenses. touch the slide you are looking at.

What is It

The two lenses that determine the magnification of the microscope are the
eyepiece lens and the objective lens. Each has a number on it that signifies its
magnification.
OCULAR LENS (eyepiece) – Your microscope will have either one (monocular)
or two (binocular) ocular lenses.
These are the lenses you will look through when examining a specimen with
the microscope. Take a look at the side of your ocular lens and you will notice a
label of “10X”. This indicates that each ocular lens magnifies the image by a factor
of 10 or 10X.
OBJECTIVE LENSES – Notice the set of objective lenses on the revolving
nosepiece. These lenses allow you to change the degree of magnification. Some of
our microscopes have four objective lenses while others have only three. The degree
of magnification for each objective lens is indicated on its side. Let’s take a look at
each progressing from the shortest to longest objective lenses, being sure to rotate
the revolving nosepiece to click each objective lens into position above the stage
before examining it:

• 4X – This objective magnifies the image by a factor of 4. It is referred to as

the “scanning objective” since it is used to scan the slide to locate the
specimen before viewing it at higher magnification.

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 • 10X – This objective magnifies the image by a factor of 10 and is referred to
as the “low power” objective. 40X – This objective magnifies the image by
a factor of 40 and is referred to as the “high power” objective.
Total Magnification
The total magnification of an image is quite simple – it is the product of the
ocular lens magnification times the magnification of the objective lens you are
using:
Magnification of Ocular x Magnification of Objective = Total Magnification
For example, if the ocular lens magnifies the image by a factor of 10 (10X),
and the objective lens magnifies the image by a factor of 50 (50X), the total
magnification of the image is 500X:
10X x 50X = 500X
Many students make the mistake of adding the two magnifications, so
remember that total magnification is the product (multiplication) of the ocular
and objective lens magnifications.

What’s More

Magnification

Directions: Fill the blanks by solving the missing numbers. Write your answer on a
separate sheet of paper.
Eyepiece Lens Objective Lens Total Magnification
5x (1) 20x
5x 40x (2)
(3) 10x 100x
10x 40x (4)

What I Have Learned

Directions: Read the paragraph carefully and identify the correct words that fit in
the given sentences in the box below. Write your answer on a separate sheet of
paper.

small arm lowest dust cover

base lenses glass lens paper

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 Microscopes are devices used to magnify (1) _________ objects, allowing them to be seen by the
naked eye. Most microscopes have several different powerful lenses attached to them, allowing
the viewer to inspect the content at more than 100 times its actual size.
Hold the microscope with one hand around the (2) ______ of the device, and the other hand
under the (3) ______. This is the most secure way to hold and walk with the microscope.

Avoid touching the (4) ________ of the microscope. The oil and dirt on your fingers
can scratch the (5) _______.
Clean smudges on the microscope glass with a piece of (6) __________. Any other
material, such as tissues or even cotton, can scratch the glass of the microscope.
Rotate the nosepiece of the microscope all the way down to its (7) ________ level when you have
finished using the microscope. If you leave the nosepiece up while storing it, the

gears on the device can wear out. If this happens, the nosepiece may not be able to lift and
hold its position.
Cover the microscope with the (8) ________ to protect it from dirt and other debris.

What I Can Do

Directions: Write 3 DO’s and 3 DON’Ts in Handling a Microscope . Write your

answer on a separate sheet of paper.

Assessment
Directions: Choose the best answer from the given choices. Write the letter of your
choice on a separate sheet of paper.
1. To obtain total magnification, you should ______ the magnification of the
eyepiece to the objective lens power.
A. add
B. divide
C. multiply
D. subtract
2. What two parts do you place your hands while carrying a microscope?
A. Arm and Base
B. Arm and Ocular
C. Base and Nosepiece
D. Base and High-Power Objective
3. What do you use to clean the microscope lenses?
A. Cotton
B. Wet Wipes
C. Lens Paper
D. Tissue Paper
4. If the eyepiece magnification on light microscope is x10 and the objective is
x40, what is the overall magnification?

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 A. 4x
B. 10x
C. 40x
D. 400x
5. The magnification of a microscope with a 10x eyepiece and a 10x objective
will be 1000x.
A. True
B. False
C. Maybe
D. Unnecessary
6. Find the magnification: Eyepiece= 10x Objective lens= 55x
A. 10x
B. 55x
C. 65x
D. 550x
7. Find the magnification: Eyepiece= 20x Objective lens= 20x
A. 20x
B. 40x
C. 200x
D. 400x
8. The higher the magnification, the ________ you see of specimen.
A. less
B. more
C. lower
D. higher
9. An increase in magnification causes _____________.
A. a decrease in the field of view
B. an increase in the working time
C. a decrease in the working distance
D. an increase in the working distance
[Link] of the following should you NOT do while using the microscope?
A. Use low power objectives lens first.
B. Use stage clips to hold the slide in place.
C. Use direct sunlight when mirror is the light source.
D. Use fine adjustment only under the high -power objective.

Additional Activities
Directions: Make a sentence that best describes a microscope from the words
given in the box. Write your answer on a separate sheet of paper.

instrument to that seen used is

A small see an
optical by to microscope
eyes our be too objects
are

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References
Carale, Lourdes R., Elvira R. Galvez, and Risa L. Reyes. 2004. Science and
Technology [Link] City: Studio Graphic Corp.
Ferguson, Greyson. 2018. Google Chrome - Sciencing - How to Handle A
Microscope. March 10. Accessed June 2, 2020.
[Link]

Ford, Brian J., and Robert R. Shannon. 2019. Google Chrome - Encyclopedia
Britannica. November 20. Accessed June 2, 2020.
[Link]

Max M. Houck, Jay A. Siegel. 2015. Google Chrome - Science Direct- Microscope.
Accessed June 2, 2020. [Link]
health-professions/microscope.

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 EDITOR’S NOTE
This Self – learning Module (SLM) was developed by the DepEd SOCCSKSARGEN
with primary objective of preparing for ang addressing the new normal. Contents of
this module were based on DepEd’s Most Essential Learning Competencies (MELC).
This is a supplementary material used by all learners of Region XII in all public
schools beginning 2020 – 2021. This process of LR development was observed in
the production of this module. This version 1.0. We highly encourage feedback,
comments and recommendations.

For inquiries or feedback, please write or call:

Department of Education – SOCCSKSARGEN

Learning Resource Management System (LRMS)

Regional Center, Brgy. Carpenter Hill, City of Koronadal

Telefax No.: (083) 2288825/ (083) 2281893

Email Address: region12@[Link]

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