unii3 Vawiumsiie 3a anuinil qunsaf 3d mani 1) Isotretinoin (CagH,,0,) 2) Clindamycin Phosphate (C, H,,CIN,0,PS) 3) Carbopol 940 (visFmionasuiniinoadifa, Useinelne) 4) Hydroxy propyl methyl cellulose (HPMC 4000) (UINmMenasuniinen ‘tiff, seinen) ‘Ine 312 Co,Ltd., USA) 5) Methyl cellulose (MC 1500) (U3vmonAsuniinoadiia, Uszinelne) 6) Triethanolamine (AJAX Finechem; Australia) 7) Bthyl alcoho! (Merk, Germany) 8) Isopropyl alcohol (Merk, Germany) . 9) Butylated hydroxytoluene (BHT) (3 MenAsunineadina, Usenet 10) Disodium ethylenediaminetetraacetic acid (EDTA) (Ui Hmienasauniinon via, Uszine’Ina) 11) Thioglycolate broth (Fluga, Switzerland) 12) Tryptic Soy Agar (Fluga, Switzerland) 13) TWEEN 20 (Sigma, USA) aunsaiuaziniosito 1) tnoedsinsev (Analytical balance, Sartorius, Germany) 2) pH meter (Model 10 NY, Accumet, USA) 3) Brookfield viscometer (Brookfield viscometer, Model DV-IIL, Brookfield 4) Sonicator (Elma Transsnonic 700/H, USA) 5) UV-spectrophotometer (Shimadzu {1 UV-1700, Japan)©) ios HPLC (Perkin Elmer series LC pump 200, USA) 7) Franz diffusion cell (Crown glass company INC model FDC-400 Improved, USA) 8) Autoclave (Biomed, USA) 9) GuaumnerouunFio Cncubator, Memmert® Germany) 32 Wauitums3is 321 wiT#GinaisvlFurw Isotretinoin Me Clindamycin Phosphate Tudviue wou mawnffanaived Isotretinoin tae Clindamycin Phosphate WinniiaTasinta na wiilveanaamussourqs (HPLC, High performance liquid chromatography) 32.11 annuntosiieil Fins noainfiilgiiniu HPLC tihuaiia Reverse phase us39H30 C-18 ‘nADyntn 4 jum, 4.6 X 150 mm Zorbax® UIN Agilent Technology) duTuIgmAaunoutt (Mobile phase) ¥O% Clindamycin Phosphate Uszn9ua2W Methanol tia Phosphate buffer pH 665 (60:40) dauigmnindoutlumsnliura Isotretinoin 13 Methanol maganduueaga arlaTonniteldiadenauerandy 214 wiluwas une 355 wiTuuasdindy Clindamycin Phosphate az Isotretinoin AINA TAU. 321.2 muafouensnemwnnsg ‘FaersanATgM Isotretinoin 110.5000 nu Idasle Volumetric flask ‘sina 100 findiGas ufanzmwludavtiarmefe Methanol se WAerisnzenwariasg iu Isotretinoin manudiidy 5000 uTasniwriniindaas vimiusonsdolitunradudu sso uTasnsi iofindas daumsazaieuinsgiu Clindamycin Phosphate inten 14 Tnomademrsuiasgay Clindamyein Phosphate 311 1.0 niu TelaaTu Volumetric flask fidluura 250 finfias uaaneaw ludarnsmofod indus Worsmvaowrasgiw Clindamyein Phosphate Huds 4000 uTnaniuroiindiins vimiuSonradotitithunausdudu 50-500 lu lnanfurodiaaias 3.2.13 nraaiounsazmumad mainioumisazmedtodhaftedins rfaarat Isotretinoin FedrFuvunfeunlseara 2.000 nf Idaslu Volumetric flask wa 100 fiadiéins udaazarelumavhazmufio Methanol ve aizazawA aod ities 1027 ‘a lnanduodingaias weheuenazmenuaudabensasaedandi hiniesawnszawnses tues t shansazmudquusnfishhissana s faffas ee Mensnemoiileftemansota hl Anse nliunadao HPLC Maunoumizazaid Dd aim TAs Zz 491M Clindamycin Phosphate Sairiuewaenlsean 2.000 nduldastu Volumetric flask wn 100 findfins udroemegavavhiavmefiodinduss ensneawm odds 200 luTasndw soiinfiins whoudfowansmwmuaudahmsnemwxindnlinsesMunszmmnsouves 1 se Rersazmoleudatah hfanmertimnlanatae HeLC 32.14 miasemounrugndosverisiinsiey (Validation of Analytical Method) mamneifeune? isavmuiaerdvigy (aloft Recovery) ludtuta freatuthaduass CLincarity) vornnudiutussentiarsuduves Isotretinoin wae CClindamycin Phosphate fu Peak area Tabinsizv4e7n Linear regression analysis uftarsari Slope Intercept tin Correlation coefficient (R’) vestduns winasgiu laelgensazatwuiAagT [sotretinoin 11% Clindamycin Phosphate 322 mafinnounsitaiiy mariannuaciseiiuqasdaiutotonsiiaunsrrandusuvesciaulsznoy wh iuniudty 3 dunoudad dunouil 1 manerumiumevestanditqyhuttowa manacouldunouiitonsamounnumiumieveansnse nef av09e301 shfqilubriueuniou nacouTaoms thariuewsatunfoutuunessylunaoanaasiiteh nmvimuvaane 3 sunnisie dumisuuvosnaoananes sumisnarnaoanaaos woe Sunniadieqavomananaaos dusaudumisaeseane 2.000 fu thuvianertin 11001 Isotretinoin tine Clindamycin Phosphate vouusiazd unis ufouiiouzounefias ay Tuidaesunis anastandaeynasiintnditesdunntunnia Tunouii2 maonyiiauarnmudatuiionnemuvosesriowa Sunowidotonniaunenmnduduvornsiomadltieadsinnunita onnzeuftes dithuominmed wall anasilénuacynala fidesowmmioy finnunsiaa oda Maeezroarnily28 isfomeattunrneanowauiaesingd 1) tGumva97N Hydroxy propyl methylcellulose (HPMC) AnususUzONAS 2,3une 4 thwntndontanin 2) wivmivaein Meryl cellulose (MC) nTUNtauzoNne 2, 3 uae 4 taniA sovinin 3) infvmangin Carbopol 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crmusaidocemuty 45 ostuvadurqamgiia: 24 daTue Tuy 1 seu dinimnadou fanun 10 300 2) narounnunmalauiuluqungineh teuatoutiumragdsuay 3 shod snlugangif 45 oamuraiue dhiam 14 Su dhumamaouseusdu thiviutidiuhofs2 anneundssihudovaememummunedinnaruns somaniinfousioumsnlaounlastuiowhnnfyluanaeiis hadtswodundollt29 Sowarmamenn 1) Sanrunitalauls Brookfield viscometer Jafigangil 25 samuvaiiun Taold Spindle uuu fi vaneiavE Warudalumsaududaus 10 sousowni dati mmualumsaungias 10 soudourfiewammidatumsmuity 140 soudowl uazan nomutatumsnundeay 10 soudourll sunaaufady 10 souriowri drusinamnedtaidorh Jounevosmadmumuusatia (alosisud Torque) fikaindifes 100 2) 4apH Tao nse Sarhinuihunsadie 3) dunadmumzifoisa une dyostriudaumnleh fimnenunedamani shévuihummdvluannsiens 2 daneliiuaoud 4 suvtianei Fu rng201 Isotretinoin tae: Clindamycin phosphate fumfongludiiu #20 HPLC-UV detector wins Validate FEnrvinsievilaumsM: nouhwFooarvesandmants 2 viialudiy Taumaisioudaodnadeioliit msiins 121 Isotretinoin > sh 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