Page 4 SuperScript™ II Reverse Transcriptase

Additional Products Cat. No. 18064-022 Size: 2,000 units

RNaseOUT™ Recombinant Ribonuclease Inhibitor (40 units/μL) is available Cat. No. 18064-014 Size: 10,000 units
separately from Invitrogen (Cat. no. 10777-019). Cat. No. 18064-071 Size: 4 × 10,000 units
Conc. 200 U/μL Store at –20°C (non-frost-free)
References
1. Kotewicz, M.L., D'Alessio, J.M., Driftmier, K.M., Blodgett, K.P., and Gerard, Description
G.F. (1985) Gene 35, 249. SuperScript™ II Reverse Transcriptase (RT) is an engineered version of M-
2. Gerard, G.F., D'Alessio, J.M., Kotewicz, M.L., and Noon, M.C. (1986) DNA 5, MLV RT with reduced RNase H activity and increased thermal stability. The
271. enzyme is purified to near homogeneity from E. coli containing the modified
3. Houts, G.E., Miyagi, M., Ellis, C., Beard, A., and Beard, J.W. (1979) J. Virol. pol gene of Moloney Murine Leukemia Virus (1,2). The enzyme can be used to
29, 517. synthesize first-strand cDNA at higher temperatures than conventional M-
4. Kotewicz, M.L., Sampson, C.M., D'Alessio, J.M., and Gerard, G.F. (1988) Nuc. MLV RT, providing increased specificity, higher yields of cDNA, and more
Acids Res. 16, 265. full-length product. It can generate cDNA up to 12.3 kb.
Limited Use Label Licenses Components
Limited Use Label License No. 5: Invitrogen Technology
The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the SuperScript™ II RT, 5X First-Strand Buffer (250 mM Tris-HCl, pH 8.3 at room
product and components of the product in research conducted by the buyer (whether the buyer is an academic or temperature; 375 mM KCl; 15 mM MgCl2), 0.1 M DTT
for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made
using this product or its components to a third party or otherwise use this product or its components or materials
made using this product or its components for Commercial Purposes. The buyer may transfer information or Storage Buffer
materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01%
Commercial Purpose, and that such collaborator agrees in writing (a) not to transfer such materials to any third
party, and (b) to use such transferred materials and/or information solely for research and not for Commercial (v/v) NP-40, 50% (v/v) glycerol
Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited
to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a Storage Conditions
service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic
purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for Store all components at –20°C in a non-frost-free freezer.
use in research. For products that are subject to multiple limited use label licenses, the terms of the most restrictive Thaw 5X First-Strand Buffer and 0.1 M DTT at room temperature just prior to
limited use label license shall control. Life Technologies Corporation will not assert a claim against the buyer of
infringement of patents owned or controlled by Life Technologies Corporation which cover this product based upon use and refreeze immediately.
the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic product developed in
research by the buyer in which this product or its components was employed, provided that neither this product nor Unit Definition
any of its components was used in the manufacture of such product. If the purchaser is not willing to accept the
limitations of this limited use statement, Life Technologies is willing to accept return of the product with a full One unit incorporates 1 nmole of dTTP into acid-precipitable material in
refund. For information about purchasing a license to use this product or the technology embedded in it for any use 10 min. at 37°C using poly(A)•oligo(dT)25 as template-primer (3).
other than for research use please contact Out Licensing, Life Technologies, 5791 Van Allen Way, Carlsbad,
California 92008 ; Phone (760) 603-7200 or e-mail: outlicensing@[Link] Intended Use
©2010 Life Technologies Corporation. All rights reserved. For research use only. Not intended for any animal or human therapeutic or
diagnostic use.
Part no. [Link] MAN0001342 Rev. Date: 20 May 2010

For technical support, email tech_support@[Link].

For country-specific contact information, visit [Link].

Page 4 SuperScript™ II Reverse Transcriptase

Additional Products Cat. No. 18064-022 Size: 2,000 units
RNaseOUT™ Recombinant Ribonuclease Inhibitor (40 units/μL) is available Cat. No. 18064-014 Size: 10,000 units
separately from Invitrogen (Cat. no. 10777-019). Cat. No. 18064-071 Size: 4 × 10,000 units
Conc. 200 U/μL Store at –20°C (non-frost-free)
References
1. Kotewicz, M.L., D'Alessio, J.M., Driftmier, K.M., Blodgett, K.P., and Gerard, Description
G.F. (1985) Gene 35, 249. SuperScript™ II Reverse Transcriptase (RT) is an engineered version of M-
2. Gerard, G.F., D'Alessio, J.M., Kotewicz, M.L., and Noon, M.C. (1986) DNA 5, MLV RT with reduced RNase H activity and increased thermal stability. The
271. enzyme is purified to near homogeneity from E. coli containing the modified
3. Houts, G.E., Miyagi, M., Ellis, C., Beard, A., and Beard, J.W. (1979) J. Virol. pol gene of Moloney Murine Leukemia Virus (1,2). The enzyme can be used to
29, 517. synthesize first-strand cDNA at higher temperatures than conventional M-
4. Kotewicz, M.L., Sampson, C.M., D'Alessio, J.M., and Gerard, G.F. (1988) Nuc. MLV RT, providing increased specificity, higher yields of cDNA, and more
Acids Res. 16, 265. full-length product. It can generate cDNA up to 12.3 kb.
Limited Use Label Licenses Components
Limited Use Label License No. 5: Invitrogen Technology
The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the SuperScript™ II RT, 5X First-Strand Buffer (250 mM Tris-HCl, pH 8.3 at room
product and components of the product in research conducted by the buyer (whether the buyer is an academic or temperature; 375 mM KCl; 15 mM MgCl2), 0.1 M DTT
for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made
using this product or its components to a third party or otherwise use this product or its components or materials
made using this product or its components for Commercial Purposes. The buyer may transfer information or Storage Buffer
materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01%
Commercial Purpose, and that such collaborator agrees in writing (a) not to transfer such materials to any third
party, and (b) to use such transferred materials and/or information solely for research and not for Commercial (v/v) NP-40, 50% (v/v) glycerol
Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited
to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a Storage Conditions
service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic
purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for Store all components at –20°C in a non-frost-free freezer.
use in research. For products that are subject to multiple limited use label licenses, the terms of the most restrictive Thaw 5X First-Strand Buffer and 0.1 M DTT at room temperature just prior to
limited use label license shall control. Life Technologies Corporation will not assert a claim against the buyer of
infringement of patents owned or controlled by Life Technologies Corporation which cover this product based upon use and refreeze immediately.
the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic product developed in
research by the buyer in which this product or its components was employed, provided that neither this product nor Unit Definition
any of its components was used in the manufacture of such product. If the purchaser is not willing to accept the
limitations of this limited use statement, Life Technologies is willing to accept return of the product with a full One unit incorporates 1 nmole of dTTP into acid-precipitable material in
refund. For information about purchasing a license to use this product or the technology embedded in it for any use 10 min. at 37°C using poly(A)•oligo(dT)25 as template-primer (3).
other than for research use please contact Out Licensing, Life Technologies, 5791 Van Allen Way, Carlsbad,
California 92008 ; Phone (760) 603-7200 or e-mail: outlicensing@[Link] Intended Use
©2010 Life Technologies Corporation. All rights reserved. For research use only. Not intended for any animal or human therapeutic or
diagnostic use.
Part no. [Link] MAN0001342 Rev. Date: 20 May 2010
 Page 2 Page 3

™ First-Strand cDNA Synthesis Using SuperScript™ II RT, Continued

First-Strand cDNA Synthesis Using SuperScript II RT The cDNA can now be used as a template for amplification in PCR. However,
A 20-μL reaction volume can be used for 1 ng–5 μg of total RNA or 1–500 ng amplification of some PCR targets (>1 kb) may require the removal of RNA
of mRNA. complementary to the cDNA. To remove RNA complementary to the cDNA,
1. Add the following components to a nuclease-free microcentrifuge tube: add 1 μL (2 units) of E. coli RNase H and incubate at 37°C for 20 min.
Oligo(dT)12-18 (500 μg/mL) or 1 μL
50–250 ng random primers or PCR
2 pmole gene-specific primer (GSP) The following is intended as a guideline and starting point when using first-
1 ng to 5 μg total RNA or x μL strand cDNA in PCR with Taq DNA polymerase. The optimal concentration of
1–500 ng of mRNA Mg++ will vary depending on the template and primer pair.
1 μL dNTP Mix (10 mM each) 1 μL Use only 10% of the first-strand reaction for PCR. Higher volumes may not
Sterile, distilled water to 12 μL increase amplification and may result in decreased amounts of PCR product.
2. Heat mixture to 65°C for 5 min and quick chill on ice. Collect the 1. Add the following to a PCR tube:
contents of the tube by brief centrifugation and add: 10X PCR Buffer [200 mM Tris-HCl (pH 8.4), 500 mM KCl] 5 μL
5X First-Strand Buffer 4 μL 50 mM MgCl2 1.5 μL
0.1 M DTT 2 μL 10 mM dNTP Mix 1 μL
RNaseOUT™ (40 units/μL) (optional)* 1 μL Forward primer (10 μM) 1 μL
Reverse primer (10 μM) 1 μL
*RNaseOUT™ (Cat. No. 10777-019) is required if using <50 ng starting RNA.
Taq DNA polymerase (5 U/μL) 0.4 μL
3. Mix contents of the tube gently. If you are using oligo(dT)12-18 or GSP, cDNA from first-strand reaction 2 μL
incubate at 42°C for 2 min. If you are using random primers, incubate at autoclaved, distilled water to 50 μL
25°C for 2 min. 2. Mix gently and layer with 1–2 drops (~50 μL) of silicone oil. (Note:
4. Add 1 μL (200 units) of SuperScript™ II RT and mix by pipetting gently silicone oil is unnecessary in thermal cyclers equipped with a heated lid.)
up and down.
3. Heat reaction to 94°C for 2 min to denature.
If you are using less than 1 ng of RNA, reduce the amount of
SuperScript™ II RT to 0.25 μL (50 units) and add sterile, distilled water to 4. Perform 15 to 40 cycles of PCR. Use the recommended annealing and
a 20 μL final volume. extension conditions for your Taq DNA polymerase.

If you are using random primers, incubate tube at 25°C for 10 min. Product Qualification
5. Incubate at 42°C for 50 min. The Certificate of Analysis provides detailed quality control information for
each product. Certificates of Analysis are available at
6. Inactivate the reaction by heating at 70°C for 15 min. [Link]/support.

Page 2 Page 3

™ First-Strand cDNA Synthesis Using SuperScript™ II RT, Continued

First-Strand cDNA Synthesis Using SuperScript II RT The cDNA can now be used as a template for amplification in PCR. However,
A 20-μL reaction volume can be used for 1 ng–5 μg of total RNA or 1–500 ng amplification of some PCR targets (>1 kb) may require the removal of RNA
of mRNA. complementary to the cDNA. To remove RNA complementary to the cDNA,
1. Add the following components to a nuclease-free microcentrifuge tube: add 1 μL (2 units) of E. coli RNase H and incubate at 37°C for 20 min.
Oligo(dT)12-18 (500 μg/mL) or 1 μL
50–250 ng random primers or PCR
2 pmole gene-specific primer (GSP) The following is intended as a guideline and starting point when using first-
1 ng to 5 μg total RNA or x μL strand cDNA in PCR with Taq DNA polymerase. The optimal concentration of
1–500 ng of mRNA Mg++ will vary depending on the template and primer pair.
1 μL dNTP Mix (10 mM each) 1 μL Use only 10% of the first-strand reaction for PCR. Higher volumes may not
Sterile, distilled water to 12 μL increase amplification and may result in decreased amounts of PCR product.
2. Heat mixture to 65°C for 5 min and quick chill on ice. Collect the 1. Add the following to a PCR tube:
contents of the tube by brief centrifugation and add: 10X PCR Buffer [200 mM Tris-HCl (pH 8.4), 500 mM KCl] 5 μL
5X First-Strand Buffer 4 μL 50 mM MgCl2 1.5 μL
0.1 M DTT 2 μL 10 mM dNTP Mix 1 μL
RNaseOUT™ (40 units/μL) (optional)* 1 μL Forward primer (10 μM) 1 μL
Reverse primer (10 μM) 1 μL
*RNaseOUT™ (Cat. No. 10777-019) is required if using <50 ng starting RNA.
Taq DNA polymerase (5 U/μL) 0.4 μL
3. Mix contents of the tube gently. If you are using oligo(dT)12-18 or GSP, cDNA from first-strand reaction 2 μL
incubate at 42°C for 2 min. If you are using random primers, incubate at autoclaved, distilled water to 50 μL
25°C for 2 min. 2. Mix gently and layer with 1–2 drops (~50 μL) of silicone oil. (Note:
4. Add 1 μL (200 units) of SuperScript™ II RT and mix by pipetting gently silicone oil is unnecessary in thermal cyclers equipped with a heated lid.)
up and down.
3. Heat reaction to 94°C for 2 min to denature.
If you are using less than 1 ng of RNA, reduce the amount of
SuperScript™ II RT to 0.25 μL (50 units) and add sterile, distilled water to 4. Perform 15 to 40 cycles of PCR. Use the recommended annealing and
a 20 μL final volume. extension conditions for your Taq DNA polymerase.

If you are using random primers, incubate tube at 25°C for 10 min. Product Qualification
5. Incubate at 42°C for 50 min. The Certificate of Analysis provides detailed quality control information for
each product. Certificates of Analysis are available at
6. Inactivate the reaction by heating at 70°C for 15 min. [Link]/support.
 Page 2 Page 3

™ First-Strand cDNA Synthesis Using SuperScript™ II RT, Continued

First-Strand cDNA Synthesis Using SuperScript II RT The cDNA can now be used as a template for amplification in PCR. However,
A 20-μL reaction volume can be used for 1 ng–5 μg of total RNA or 1–500 ng amplification of some PCR targets (>1 kb) may require the removal of RNA
of mRNA. complementary to the cDNA. To remove RNA complementary to the cDNA,
1. Add the following components to a nuclease-free microcentrifuge tube: add 1 μL (2 units) of E. coli RNase H and incubate at 37°C for 20 min.
Oligo(dT)12-18 (500 μg/mL) or 1 μL
50–250 ng random primers or PCR
2 pmole gene-specific primer (GSP) The following is intended as a guideline and starting point when using first-
1 ng to 5 μg total RNA or x μL strand cDNA in PCR with Taq DNA polymerase. The optimal concentration of
1–500 ng of mRNA Mg++ will vary depending on the template and primer pair.
1 μL dNTP Mix (10 mM each) 1 μL Use only 10% of the first-strand reaction for PCR. Higher volumes may not
Sterile, distilled water to 12 μL increase amplification and may result in decreased amounts of PCR product.
2. Heat mixture to 65°C for 5 min and quick chill on ice. Collect the 1. Add the following to a PCR tube:
contents of the tube by brief centrifugation and add: 10X PCR Buffer [200 mM Tris-HCl (pH 8.4), 500 mM KCl] 5 μL
5X First-Strand Buffer 4 μL 50 mM MgCl2 1.5 μL
0.1 M DTT 2 μL 10 mM dNTP Mix 1 μL
RNaseOUT™ (40 units/μL) (optional)* 1 μL Forward primer (10 μM) 1 μL
Reverse primer (10 μM) 1 μL
*RNaseOUT™ (Cat. No. 10777-019) is required if using <50 ng starting RNA.
Taq DNA polymerase (5 U/μL) 0.4 μL
3. Mix contents of the tube gently. If you are using oligo(dT)12-18 or GSP, cDNA from first-strand reaction 2 μL
incubate at 42°C for 2 min. If you are using random primers, incubate at autoclaved, distilled water to 50 μL
25°C for 2 min. 2. Mix gently and layer with 1–2 drops (~50 μL) of silicone oil. (Note:
4. Add 1 μL (200 units) of SuperScript™ II RT and mix by pipetting gently silicone oil is unnecessary in thermal cyclers equipped with a heated lid.)
up and down.
3. Heat reaction to 94°C for 2 min to denature.
If you are using less than 1 ng of RNA, reduce the amount of
SuperScript™ II RT to 0.25 μL (50 units) and add sterile, distilled water to 4. Perform 15 to 40 cycles of PCR. Use the recommended annealing and
a 20 μL final volume. extension conditions for your Taq DNA polymerase.

If you are using random primers, incubate tube at 25°C for 10 min. Product Qualification
5. Incubate at 42°C for 50 min. The Certificate of Analysis provides detailed quality control information for
each product. Certificates of Analysis are available at
6. Inactivate the reaction by heating at 70°C for 15 min. [Link]/support.

Page 2 Page 3

™ First-Strand cDNA Synthesis Using SuperScript™ II RT, Continued

First-Strand cDNA Synthesis Using SuperScript II RT The cDNA can now be used as a template for amplification in PCR. However,
A 20-μL reaction volume can be used for 1 ng–5 μg of total RNA or 1–500 ng amplification of some PCR targets (>1 kb) may require the removal of RNA
of mRNA. complementary to the cDNA. To remove RNA complementary to the cDNA,
1. Add the following components to a nuclease-free microcentrifuge tube: add 1 μL (2 units) of E. coli RNase H and incubate at 37°C for 20 min.
Oligo(dT)12-18 (500 μg/mL) or 1 μL
50–250 ng random primers or PCR
2 pmole gene-specific primer (GSP) The following is intended as a guideline and starting point when using first-
1 ng to 5 μg total RNA or x μL strand cDNA in PCR with Taq DNA polymerase. The optimal concentration of
1–500 ng of mRNA Mg++ will vary depending on the template and primer pair.
1 μL dNTP Mix (10 mM each) 1 μL Use only 10% of the first-strand reaction for PCR. Higher volumes may not
Sterile, distilled water to 12 μL increase amplification and may result in decreased amounts of PCR product.
2. Heat mixture to 65°C for 5 min and quick chill on ice. Collect the 1. Add the following to a PCR tube:
contents of the tube by brief centrifugation and add: 10X PCR Buffer [200 mM Tris-HCl (pH 8.4), 500 mM KCl] 5 μL
5X First-Strand Buffer 4 μL 50 mM MgCl2 1.5 μL
0.1 M DTT 2 μL 10 mM dNTP Mix 1 μL
RNaseOUT™ (40 units/μL) (optional)* 1 μL Forward primer (10 μM) 1 μL
Reverse primer (10 μM) 1 μL
*RNaseOUT™ (Cat. No. 10777-019) is required if using <50 ng starting RNA.
Taq DNA polymerase (5 U/μL) 0.4 μL
3. Mix contents of the tube gently. If you are using oligo(dT)12-18 or GSP, cDNA from first-strand reaction 2 μL
incubate at 42°C for 2 min. If you are using random primers, incubate at autoclaved, distilled water to 50 μL
25°C for 2 min. 2. Mix gently and layer with 1–2 drops (~50 μL) of silicone oil. (Note:
4. Add 1 μL (200 units) of SuperScript™ II RT and mix by pipetting gently silicone oil is unnecessary in thermal cyclers equipped with a heated lid.)
up and down.
3. Heat reaction to 94°C for 2 min to denature.
If you are using less than 1 ng of RNA, reduce the amount of
SuperScript™ II RT to 0.25 μL (50 units) and add sterile, distilled water to 4. Perform 15 to 40 cycles of PCR. Use the recommended annealing and
a 20 μL final volume. extension conditions for your Taq DNA polymerase.

If you are using random primers, incubate tube at 25°C for 10 min. Product Qualification
5. Incubate at 42°C for 50 min. The Certificate of Analysis provides detailed quality control information for
each product. Certificates of Analysis are available at
6. Inactivate the reaction by heating at 70°C for 15 min. [Link]/support.
 Page 4 SuperScript™ II Reverse Transcriptase
Additional Products Cat. No. 18064-022 Size: 2,000 units
RNaseOUT™ Recombinant Ribonuclease Inhibitor (40 units/μL) is available Cat. No. 18064-014 Size: 10,000 units
separately from Invitrogen (Cat. no. 10777-019). Cat. No. 18064-071 Size: 4 × 10,000 units
Conc. 200 U/μL Store at –20°C (non-frost-free)
References
1. Kotewicz, M.L., D'Alessio, J.M., Driftmier, K.M., Blodgett, K.P., and Gerard, Description
G.F. (1985) Gene 35, 249. SuperScript™ II Reverse Transcriptase (RT) is an engineered version of M-
2. Gerard, G.F., D'Alessio, J.M., Kotewicz, M.L., and Noon, M.C. (1986) DNA 5, MLV RT with reduced RNase H activity and increased thermal stability. The
271. enzyme is purified to near homogeneity from E. coli containing the modified
3. Houts, G.E., Miyagi, M., Ellis, C., Beard, A., and Beard, J.W. (1979) J. Virol. pol gene of Moloney Murine Leukemia Virus (1,2). The enzyme can be used to
29, 517. synthesize first-strand cDNA at higher temperatures than conventional M-
4. Kotewicz, M.L., Sampson, C.M., D'Alessio, J.M., and Gerard, G.F. (1988) Nuc. MLV RT, providing increased specificity, higher yields of cDNA, and more
Acids Res. 16, 265. full-length product. It can generate cDNA up to 12.3 kb.
Limited Use Label Licenses Components
Limited Use Label License No. 5: Invitrogen Technology
The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the SuperScript™ II RT, 5X First-Strand Buffer (250 mM Tris-HCl, pH 8.3 at room
product and components of the product in research conducted by the buyer (whether the buyer is an academic or temperature; 375 mM KCl; 15 mM MgCl2), 0.1 M DTT
for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made
using this product or its components to a third party or otherwise use this product or its components or materials
made using this product or its components for Commercial Purposes. The buyer may transfer information or Storage Buffer
materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01%
Commercial Purpose, and that such collaborator agrees in writing (a) not to transfer such materials to any third
party, and (b) to use such transferred materials and/or information solely for research and not for Commercial (v/v) NP-40, 50% (v/v) glycerol
Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited
to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a Storage Conditions
service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic
purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for Store all components at –20°C in a non-frost-free freezer.
use in research. For products that are subject to multiple limited use label licenses, the terms of the most restrictive Thaw 5X First-Strand Buffer and 0.1 M DTT at room temperature just prior to
limited use label license shall control. Life Technologies Corporation will not assert a claim against the buyer of
infringement of patents owned or controlled by Life Technologies Corporation which cover this product based upon use and refreeze immediately.
the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic product developed in
research by the buyer in which this product or its components was employed, provided that neither this product nor Unit Definition
any of its components was used in the manufacture of such product. If the purchaser is not willing to accept the
limitations of this limited use statement, Life Technologies is willing to accept return of the product with a full One unit incorporates 1 nmole of dTTP into acid-precipitable material in
refund. For information about purchasing a license to use this product or the technology embedded in it for any use 10 min. at 37°C using poly(A)•oligo(dT)25 as template-primer (3).
other than for research use please contact Out Licensing, Life Technologies, 5791 Van Allen Way, Carlsbad,
California 92008 ; Phone (760) 603-7200 or e-mail: outlicensing@[Link] Intended Use
©2010 Life Technologies Corporation. All rights reserved. For research use only. Not intended for any animal or human therapeutic or
diagnostic use.
Part no. [Link] MAN0001342 Rev. Date: 20 May 2010

Page 4 SuperScript™ II Reverse Transcriptase

Additional Products Cat. No. 18064-022 Size: 2,000 units
RNaseOUT™ Recombinant Ribonuclease Inhibitor (40 units/μL) is available Cat. No. 18064-014 Size: 10,000 units
separately from Invitrogen (Cat. no. 10777-019). Cat. No. 18064-071 Size: 4 × 10,000 units
Conc. 200 U/μL Store at –20°C (non-frost-free)
References
1. Kotewicz, M.L., D'Alessio, J.M., Driftmier, K.M., Blodgett, K.P., and Gerard, Description
G.F. (1985) Gene 35, 249. SuperScript™ II Reverse Transcriptase (RT) is an engineered version of M-
2. Gerard, G.F., D'Alessio, J.M., Kotewicz, M.L., and Noon, M.C. (1986) DNA 5, MLV RT with reduced RNase H activity and increased thermal stability. The
271. enzyme is purified to near homogeneity from E. coli containing the modified
3. Houts, G.E., Miyagi, M., Ellis, C., Beard, A., and Beard, J.W. (1979) J. Virol. pol gene of Moloney Murine Leukemia Virus (1,2). The enzyme can be used to
29, 517. synthesize first-strand cDNA at higher temperatures than conventional M-
4. Kotewicz, M.L., Sampson, C.M., D'Alessio, J.M., and Gerard, G.F. (1988) Nuc. MLV RT, providing increased specificity, higher yields of cDNA, and more
Acids Res. 16, 265. full-length product. It can generate cDNA up to 12.3 kb.
Limited Use Label Licenses Components
Limited Use Label License No. 5: Invitrogen Technology
The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the SuperScript™ II RT, 5X First-Strand Buffer (250 mM Tris-HCl, pH 8.3 at room
product and components of the product in research conducted by the buyer (whether the buyer is an academic or temperature; 375 mM KCl; 15 mM MgCl2), 0.1 M DTT
for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made
using this product or its components to a third party or otherwise use this product or its components or materials
made using this product or its components for Commercial Purposes. The buyer may transfer information or Storage Buffer
materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01%
Commercial Purpose, and that such collaborator agrees in writing (a) not to transfer such materials to any third
party, and (b) to use such transferred materials and/or information solely for research and not for Commercial (v/v) NP-40, 50% (v/v) glycerol
Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited
to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a Storage Conditions
service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic
purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for Store all components at –20°C in a non-frost-free freezer.
use in research. For products that are subject to multiple limited use label licenses, the terms of the most restrictive Thaw 5X First-Strand Buffer and 0.1 M DTT at room temperature just prior to
limited use label license shall control. Life Technologies Corporation will not assert a claim against the buyer of
infringement of patents owned or controlled by Life Technologies Corporation which cover this product based upon use and refreeze immediately.
the manufacture, use or sale of a therapeutic, clinical diagnostic, vaccine or prophylactic product developed in
research by the buyer in which this product or its components was employed, provided that neither this product nor Unit Definition
any of its components was used in the manufacture of such product. If the purchaser is not willing to accept the
limitations of this limited use statement, Life Technologies is willing to accept return of the product with a full One unit incorporates 1 nmole of dTTP into acid-precipitable material in
refund. For information about purchasing a license to use this product or the technology embedded in it for any use 10 min. at 37°C using poly(A)•oligo(dT)25 as template-primer (3).
other than for research use please contact Out Licensing, Life Technologies, 5791 Van Allen Way, Carlsbad,
California 92008 ; Phone (760) 603-7200 or e-mail: outlicensing@[Link] Intended Use
©2010 Life Technologies Corporation. All rights reserved. For research use only. Not intended for any animal or human therapeutic or
diagnostic use.
Part no. [Link] MAN0001342 Rev. Date: 20 May 2010