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Phytochemical Screening of Ruellia tuberosa

The phytochemical screening of the Ruellia tuberosa stem ethanol extract revealed the presence of alkaloids, flavonoids, glycosides, saponins, and terpenoids but no presence of tannins. Testing showed the ethanol extract had antibacterial activity against E. coli, with a mean zone of inhibition of 13.67mm. Streptomycin, used as a positive control, had the highest antibacterial activity with a mean zone of 33.33mm, while ethanol as a negative control showed no activity.

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51 views2 pages

Phytochemical Screening of Ruellia tuberosa

The phytochemical screening of the Ruellia tuberosa stem ethanol extract revealed the presence of alkaloids, flavonoids, glycosides, saponins, and terpenoids but no presence of tannins. Testing showed the ethanol extract had antibacterial activity against E. coli, with a mean zone of inhibition of 13.67mm. Streptomycin, used as a positive control, had the highest antibacterial activity with a mean zone of 33.33mm, while ethanol as a negative control showed no activity.

Uploaded by

Apuya Calle Rielle
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Chapter 4

Results and Discussion

Phytochemical Screening

The phytochemical screening of the Ruellia tuberosa stem ethanol extract is

shown in the Table 1. The result of the phytochemical screening reveals the presence of

alkaloids, flavonoids, glycosides, saponins, and terpenoids but no presence of tannins.

Table 1. Phytochemicals of Ruellia tuberosa stem.

Phytochemicals Results
Alkaloids Present
Flavonoids Present
Glycosides Present
Saponins Present
Tannins Absent
Terpenoids Present

Antibacterial Testing

The ethanol extract of Ruellia tuberosa stem showed antibacterial activity against

E. [Link] result is presented in Table 2. Streptomycin showed the highest antibacterial

activity against [Link] (33.33mm diameter of zone of inhibition. Ethanol stem extract of

Ruellia tuberosa showed a high antibacterial activity against E. coli (13.67mm diameter

of zone of inhibition). Ethanol did not show antibacterial activity against [Link].
Table 2. Diameter zone of inhibition of R. tuberosa stem ethanol extract against E. coli.

Sample Diameter Zone of Inhibition


Replicate 1 Replicate 2 Replicate 3 Mean
T1 = R. tuberosa extract 13.0 14.5 13.5 13.67b
T2 = Streptomycin (+) 34.0 33.5 32.5 33.33a
T3 = Ethanol (-) 0 0 0 0.00c

T1
T3

T2

Figure 1. Plate assay of Ruellia tuberosa stem extract against E. coli.

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