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Microscope Operation and Wet-Mount Guide

The document summarizes the basic parts and use of a microscope. It lists the main components as the ocular lens, body tube, revolving nosepiece, arm, objective lenses, stage, stage clips, coarse adjustment knob, fine adjustment knob, light, and base. It instructs on how to carry a microscope safely and calculate magnification by multiplying the ocular and objective powers. Finally, it provides directions for examining slides using different objectives and making a simple wet mount slide.

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uytud6tid67
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9 views7 pages

Microscope Operation and Wet-Mount Guide

The document summarizes the basic parts and use of a microscope. It lists the main components as the ocular lens, body tube, revolving nosepiece, arm, objective lenses, stage, stage clips, coarse adjustment knob, fine adjustment knob, light, and base. It instructs on how to carry a microscope safely and calculate magnification by multiplying the ocular and objective powers. Finally, it provides directions for examining slides using different objectives and making a simple wet mount slide.

Uploaded by

uytud6tid67
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

T.

Trimpe 2005
[Link]
Ocular lens
(Eyepiece)
Body Tube

Nosepiece
Arm
Objectives

Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light

Base

Always carry a microscope with one hand holding the arm and
one hand under the base.
Ocular lens
Body Tube

Revolving Nosepiece
Arm
Objective Lens

Stage
Stage Clips
Coarse adjustment knob
Diaphragm
Fine adjustment knob
Light

Base
What’s my power?
To calculate the power of magnification, multiply the power of the ocular lens by the
power of the objective.

What are the powers of magnification


for each of
the objectives we have on our
microscopes?
Fill in the table on
your worksheet.
Comparing Powers of Magnification

We can see better details with higher the powers of


magnification, but we cannot see as much of the image.

Which of these images would be


viewed at a higher power of
magnification?
Let’s give it a try ...
1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective
into place.

2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment
knob (large knob) to get it the image into view and then use the fine adjustment knob (small
knob) to make it clearer.

3 – Once you have the image in view, rotate the nosepiece to view it under different powers.
Draw what you see on your worksheet!

Be careful with the largest objective! Sometimes there is not enough room
and you will not be able to use it!

4 – When you are done, turn off the microscope and put up the slides you used.
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water
will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.

4 - Slowly lower the cover slip on top of the drop.

Cover Lower slowly


Slip

5 – Place the slide on the stage and view it first with the red-banded objective. Once you
see the image, you can rotate the nosepiece to view the slide with the different objectives.

You do not need to use the stage clips when


viewing wet-mount slides!

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