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Light Microscope Usage Guide

This laboratory manual provides instructions on how to use a light microscope, including identifying its main parts like the objectives, revolving nosepiece, and coarse adjustment knob. The document explains that a light microscope uses lenses to magnify objects being observed, making their structures appear larger, and discusses the objectives of learning how to properly handle, focus, and use the microscope to study samples. Students are guided through the steps of obtaining a microscope, cleaning it, identifying its parts, preparing samples, focusing the microscope, and computing magnifications of observed objects.

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25 views2 pages

Light Microscope Usage Guide

This laboratory manual provides instructions on how to use a light microscope, including identifying its main parts like the objectives, revolving nosepiece, and coarse adjustment knob. The document explains that a light microscope uses lenses to magnify objects being observed, making their structures appear larger, and discusses the objectives of learning how to properly handle, focus, and use the microscope to study samples. Students are guided through the steps of obtaining a microscope, cleaning it, identifying its parts, preparing samples, focusing the microscope, and computing magnifications of observed objects.

Uploaded by

Zephie
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd

Laboratory Manual

Gen Sci Intensive Laboratory in Science Education

Name: Date Performed:


Subject: Date Finished:
Professor: Remarks:

How to Use the Light Microscope

I. Discussion/method/theory

The microscope is a tool which can help you see tiny objects and living
organisms. It makes them look bigger. This ability of the microscope is called
its magnifying power or magnification. The microscope also has the capacity
to distinguish small gaps between two separate points which humans cannot
distinguish. It is called its resolving power or resolution. The light microscope
uses diffused light from the sun or artificial light to illuminate the object to be
observed. From its source, visible light passes through the small or thin
specimen to be observed through the glass lenses. As light passes through
the lenses, it is bent so specimen appears bigger when it is projected to the
eye. The form and structure of the specimen can then be seen because some
of their parts reflect light.

II. Objectives

After performing this activity, you should be able to:

1. handle the microscope properly;


2. identify the parts of the microscope;
3. describe what parts of the microscope can do;
4. prepare materials for microscope study;
5. focus the microscope properly;
6. compare the image of the object seen by the unaided eye and under the
microscope; and
7. compute for the magnification of objects observed under the microscope.

III. Material & Apparatus

lens paper pencil


light microscope dropper
tissue paper or old t-shirt scissors
newspaper page tap water
glass slide and cover slips forceps or tweezer
IV. Data Procedure

1. Get the microscope from its box or the cabinet. Do this by grasping the curved
arm with one hand and supporting the base with the other hand.
2. Carry it to your table or working place. Remember to always use both hands
when carrying the microscope.
3. Put the microscope down gently on the laboratory table with its arm facing you.
Place it about 7 centimeters away from the edge of the table.
4. Wipe with tissue paper or old t-shirt the metal parts of the microscope.
5. Figure 1 shows a light microscope that most schools have. Study and use this to
locate different parts of the microscope.
6. Look for the revolving nosepiece. Note that objectives are attached it. You should
know that there are lenses inside the objectives.
7. Find the coarse adjustment. Slowly turn it upwards, then downwards.

V. Data and Results/Observations


VI. Computations/Analysis
VII. Generalization/conclusion

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